Succinyl rotundic acid inhibits growth and promotes apoptosis in the HeLa cervical cancer cell line

2020 ◽  
Vol 10 (4) ◽  
pp. 571-577
Author(s):  
Yufang He ◽  
Jiaming Sun ◽  
Minlun Nan ◽  
Xue Wang ◽  
He Lin ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Hela Cells ◽  
Water Solubility ◽  
B Cell Lymphoma ◽  
Lipid Lowering ◽  
Cervical Cancer Cell Line ◽  
Pentacyclic Triterpene ◽  
Rotundic Acid

Rotundic acid (RA) is a kind of pentacyclic triterpene saponins, which widely exists in holly and other plants. It has anti-tumor and lipid-lowering effects, and has been widely used in the prevention and treatment of vascular diseases. Succinyl RA (SRA) is a kind of circular acid derivative with strong water solubility. However, SRA’s effect and mechanism about the therapy of cervical cancer is still not clarified. The inhibitory effect of SRA on Hela cells and its molecular mechanism were investigated in this study. Hela cell line was used to establish an in vitro model to study the effect of SRA. The effects of SRA on cell proliferation, cell cycle and apoptosis were analyzed by flow cytometry and detected by 3-(4,5-dimethyl thiazole-2-yl)-2, 5-diphenyltetrazole bromide. The experimental results show that SRA can inhibit the growth of Hela and diffusion, inducing cell apoptosis. SRA can also reduce the expression of CyclinD 1 and p21. It was also decreased the expression of apoptosis inhibitor B cell lymphoma (Bcl-2), and increased the expression of Bcl-2-related X-protein and Caspase-3. This study provides evidence for the progress of SRA in inhibiting Hela cells and presents potential applications of SRA as an alternative therapy for cervical cancer.

scholarly journals An in vitro Evaluation of Apigenin and Apigenin-7-O-glucoside Against HeLa Human Cervical Cancer Cell Line

2020 ◽  
Vol 71 (2) ◽  
pp. 140-144
Author(s):  
Daliana Minda ◽  
Stefana Avram ◽  
Ioana Zinuca Pavel ◽  
Brigitta Kis ◽  
Alexandra Ghitu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Experimental Conditions ◽  
Human Cervical Cancer Cell ◽  
Cervical Cancer Cells ◽  
Apoptotic Effects ◽  
Human Cervical Cancer

pigenin (API) is a phytocompound belonging to the subclass of flavones that can be found in both functional foods as well as medicinal plants. Recent studies have assigned API antioxidant, anti-inflammatory, anti-spasmodic, anti-viral, anti-thrombotic, anti-angiogenic and chemopreventive potential in vitro on various cell lines and/or in experimental animal models. Apigenin-7-O-glucoside (API-7) is one of its main glycosides and can be commonly found in chamomile flowers, parsley, celery. The aim of this study was to evaluate the in vitro anti-proliferative and pro-apoptotic effects of API and its glycoside (apigenin-7-O-glucoside) against HeLa human cervical cancer cells. Results have shown that in the set experimental conditions both the aglycone as well as the heteroside elicit antiproliferative and pro-apoptotic potential against the screened cell line, the aglycone being more active than the heteroside.

scholarly journals Eugenol Exerts Apoptotic Effect and Modulates the Sensitivity of HeLa Cells to Cisplatin and Radiation

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3979 ◽  
Author(s):  
Moustafa Fathy ◽  
Michael Atef Fawzy ◽  
Henning Hintzsche ◽  
Toshio Nikaido ◽  
Thomas Dandekar ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Caspase 3 ◽  
Synergistic Effects ◽  
Interleukin 1 ◽  
B Cell Lymphoma ◽  
Cervical Cancer Cell Line ◽  
Dependent Manner ◽  
X Rays ◽  
Human Cervical Cancer

Eugenol is a phytochemical present in different plant products, e.g., clove oil. Traditionally, it is used against a number of different disorders and it was suggested to have anticancer activity. In this study, the activity of eugenol was evaluated in a human cervical cancer (HeLa) cell line and cell proliferation was examined after treatment with various concentrations of eugenol and different treatment durations. Cytotoxicity was tested using lactate dehydrogenase (LDH) enzyme leakage. In order to assess eugenol’s potential to act synergistically with chemotherapy and radiotherapy, cell survival was calculated after eugenol treatment in combination with cisplatin and X-rays. To elucidate its mechanism of action, caspase-3 activity was analyzed and the expression of various genes and proteins was checked by RT-PCR and western blot analyses. Eugenol clearly decreased the proliferation rate and increased LDH release in a concentration- and time-dependent manner. It showed synergistic effects with cisplatin and X-rays. Eugenol increased caspase-3 activity and the expression of Bax, cytochrome c (Cyt-c), caspase-3, and caspase-9 and decreased the expression of B-cell lymphoma (Bcl)-2, cyclooxygenase-2 (Cox-2), and interleukin-1 beta (IL-1β) indicating that eugenol mainly induced cell death by apoptosis. In conclusion, eugenol showed antiproliferative and cytotoxic effects via apoptosis and also synergism with cisplatin and ionizing radiation in the human cervical cancer cell line.

scholarly journals CYTOTOXICITY AND APOPTOSIS INDUCTION BY KAFFIR LIME LEAVES EXTRACT (Citrus hystrix DC.) IN HeLa CELLS CULTURE (HUMAN CERVICAL CANCER CELL LINE)

2015 ◽  
Vol 2 (1) ◽  
pp. 631 ◽  
Author(s):  
Nastiti Wijayanti ◽  
W.A.S. Tunjung ◽  
Y. Setyawati
Keyword(s):  
Cervical Cancer ◽  
Ethyl Acetate ◽  
Cell Line ◽  
Cancer Cell ◽  
Hela Cells ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela

<p>Kaffir lime (Citrus hystrix) is a native plant of Indonesia. Nowadays the use of plants is limited as ingredients in Indonesia cuisine and aromatherapy oils for industry. Leaves of C. hystrix contain of polyphenols and essential oils thus they were possibly cytotoxic to cancer cell line. The objective of this research was to determine the cytotoxicity effect and apoptosis induced by leaves extract C. hystrix to cervical cancer cell line (HeLa cells). Methods used in this study included sampling of kaffir lime leaves, extractionusing three different solvents (ethanol, ethyl acetate, and hexane), detection of metabolite secondary compound (alkaloids, flavonoids, terpenoids, saponins, and tannins) using thin layer chromatography (TLC), cytotoxicity assay via MTT assay, and apoptosis test with double staining method (ethidium bromide-acrydine orange). Result showed kaffir lime crude extract dose dependently inhibitHeLa cells proliferation. lC50of ethanolicand ethyl acetate extract was 82,034 µg/mL and 57,845 µg/mL, respectively means cytotoxic to HeLa cells. On the other hand lC50 of hexane extract was 203,992 µg/mL which was not cytotoxic. Furthermore ethanolic and ethyl acetate extract were able to induce apoptosis of HeLa cells by increasing the number of apoptotic cells. In conclusion, kaffir lime leaves extract had cytotoxic effect and induced apoptosis. Moreover ethyl acetate extractof kaffir lime was the most potential to induce apoptosis in HeLa cells. </p><p><strong>Keywords</strong>: kaffir lime leaves (Citrus hystrix), HeLa, MTT, cytotoxicity, apoptosis.</p>

scholarly journals CELLULAR UPTAKE STUDY AND CYTOTOXICITY STUDY OF RESVERATROL-GOLD-PEG-FOLATE (RSV-AU-PEG-FA) NANOPARTICLES ON HELA HUMAN CERVICAL CANCER CELL LINE

2020 ◽  
pp. 113-118
Author(s):  
DARA A. PUTRI ◽  
SUTRIYO SUTRIYO ◽  
FADLINA C. SAPUTRI
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cellular Uptake ◽  
Hela Cells ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Uptake Study ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in resveratrol (RSV) targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line. Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bis-amine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Cellular uptake study has shown that RSV-Au-PEG-FA nanoparticles are potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA nanoparticles IC50 67.06±2.14 mM and RSV IC50 9.66±1.44 mM on HeLa cells Conclusion: RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively.

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