Highly Sensitive Gold Nanoparticle Polymerase Chain Reaction in the Detection of Anaplastic Lymphoma Kinase-Positive Gastric Cancer from a Biopsy Specimen

2020 ◽  
Vol 12 (4) ◽  
pp. 498-505
Author(s):  
Dan Zhang ◽  
Zhaoxi Lu ◽  
Bing Sun
Keyword(s):  
Gastric Cancer ◽  
Polymerase Chain Reaction ◽  
Detection Rate ◽  
Target Gene ◽  
Malignant Tumors ◽  
Chain Reaction ◽  
Nano Gold ◽  
Biopsy Tissue ◽  
Pcr Technology ◽  
Polymerase Chain

Gastric cancer is a malignant tumor that is originated from the epithelia of the gastric mucosa. Although the gastroscopic biopsy of suspicious gastric areas can provide better early-warning opportunities for patients with malignant tumors that result in achieving early diagnosis and treatment, many malignant tumors are still excluded due to atypical histology, sampling errors, nonspecific antibodies, and other reasons, which pose a threat to the physical and mental health of patients. Therefore, more sensitive and specific detection methods of gastric cancer are needed to improve the screening efficiency of gastroscopic biopsy. The sensitivity of nano PCR was 10 times higher than that of conventional PCR. In comparison with the conventional RT-PCR method, nano PCR technology can amplify brighter bands and identify higher gene expression levels for ALK weak positive gastric cancer in IHC, and improve the detection rate of clinical specimens with lower ALK staining. Therefore, nano-gold polymerase chain reaction used gold nanoparticle (nano-gold PCR) has high sensitivity and positive detection rate for ALK-positive gastric cancer identified by gastroscopy. When a low concentration was observed for the amplified gene, especially when the biopsy tissue was too small to carry out IHC staining, the target gene could be amplified more effectively. Therefore, nano PCR technology is proposed to be widely used in target gene detection of biopsy tissue to achieve better tumor screening.

scholarly journals Development of specific primers for the detection of HVA1 from barley in transgenic durum wheat by polymerase chain reaction (PCR) technology

2014 ◽  
Vol 13 (4) ◽  
pp. 581-592 ◽  
Author(s):  
Melloul Marouane ◽  
Iraqi Driss ◽  
M Udupa Sripada ◽  
Abdelaziz El Alaoui My ◽  
Amine Alaoui Sanaa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Durum Wheat ◽  
Chain Reaction ◽  
Specific Primers ◽  
Pcr Technology ◽  
Polymerase Chain

scholarly journals Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy

2020 ◽  
Vol 8 (3) ◽  
pp. 324
Author(s):  
Sonia Toracchio ◽  
Rosario Alberto Caruso ◽  
Silvia Perconti ◽  
Luciana Rigoli ◽  
Enrico Betri ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
High Risk ◽  
Intraepithelial Neoplasia ◽  
Northern Italy ◽  
Chain Reaction ◽  
Risk Area ◽  
High Risk Area ◽  
Polymerase Chain

Helicobacter pylori (Hp) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious Hp strains. Focusing on a high GC risk area of Northern Italy (Cremona, Lombardy) and using archived paraffin-embedded biopsies, we investigated the associations between the Hp vacA and cagA genotype variants and gastric intraepithelial neoplasia (GIN, 33 cases) versus non-neoplastic gastroduodenal lesions (NNGDLs, 37 cases). The glmM gene and the cagA and vacA (s and m) genotypes were determined by polymerase chain reaction (PCR) and sequencing. Hp was confirmed in 37/37 (100%) NNGDLs and detected in 9/33 GINs (27%), consistently with the well-known Hp loss in GC. CagA was detected in 4/9 Hp-positive GINs and in 29/37 NNGDLs. The vacA s1a and m1 subtypes were more common in GINs than in NNGDLs (6/7 vs. 12/34, p=0.014, for s1a; 7/7 vs. 18/34, p=0.020 for m1), with significant vacA s genotype-specific variance. The GIN-associated vacA s1a sequences clustered together, suggesting that aggressive Hp strains from a unique founder contribute to GC in the high-risk area studied.

scholarly journals Performance of a Multiplex Nested Polymerase Chain Reaction in Detecting 7 Pathogens Containing DNA in Their Genomes Associated With Congenital Infections

2019 ◽  
Vol 144 (1) ◽  
pp. 99-106
Author(s):  
Lidia Yamamoto ◽  
Antonio G. Amorim Filho ◽  
Joelma A. Queiroz ◽  
Mario H. B. de Carvalho ◽  
Jonatas C. Rodrigues ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nested Pcr ◽  
Detection Rate ◽  
Simultaneous Detection ◽  
Immunoglobulin M ◽  
Fisher Exact Test ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Congenital Infections ◽  
Polymerase Chain

Context.— Infections are the leading cause of perinatal and infant mortality in low-income and low-resource countries, which have a higher prevalence of infections. Definitive diagnosis of congenital and perinatal infections is largely dependent upon the results of laboratory tests. Objective.— To develop a multiplex nested polymerase chain reaction (PCR) technique for the simultaneous detection of 7 pathogens containing DNA in their genomes in suspected cases of congenital infection. Design.— Eligible participants were pregnant women with positive immunoglobulin M antibodies raised to one of the pathogens in the prenatal serologic screening, associated or not with fetal ultrasound abnormalities or positive fetal serology. Neonates whose mothers did not attend prenatal care were included when they presented with symptomatology and laboratory parameters suggestive of infection. The detection rate of the multiplex nested PCR was compared with maternal, fetal, and neonatal serology, as well as placental immunohistochemistry and noncommercial amplifications. Results.— Of 161 suspected cases, the multiplex nested PCR detected 60 (37.3%), whereas the tests available in hospital laboratories detected 13 of 60 (21.7%) of the cases detected by the multiplex nested PCR, demonstrating a 4.6 times higher detection rate for the multiplex nested PCR (Fisher exact test, P < .001). Positive amplifications were to Toxoplasma gondii (32 cases), cytomegalovirus (14 cases), parvovirus B19 (5 cases), and adenovirus (5 cases). In 4 cases, 2 pathogens were simultaneously detected. All types of biological matrices were suitable for amplification. Sequencing of multiplex nested PCR products confirmed the molecular findings. Conclusions.— The multiplex nested PCR significantly increased the number of diagnosed congenital infections. Given the scarcity of DNA recovered from amniotic fluid and some neonatal samples, this multiplex nested PCR allows the simultaneous detection of 7 pathogens associated with congenital infections in a reliable, faster, cost-effective, and more sensitive way.

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