Clinical Analysis of Human Papillomavirus Infection Types Distribution in Tissues of Cervical Squamous Cell Carcinoma

2020 ◽  
Vol 12 (7) ◽  
pp. 921-927
Author(s):  
Xiumei Zhang ◽  
Xianhai Zhu ◽  
Xiaolan Xu ◽  
Chengfu Sun ◽  
Jianxiang Geng ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Infection Rate ◽  
Squamous Cell ◽  
Eastern China ◽  
Cervical Squamous Cell Carcinoma ◽  
Hpv Infection ◽  
Squamous Cell Carcinomas ◽  
Hpv 16 ◽  
Tissue Samples

In this article, we investigated prevalence and distributed types of human papillomavirus (HPV) among female cervical squamous cell carcinoma patients in eastern China (mainly in Jiangsu Province). Tissue samples of total 1021 cases with cervical squamous cell carcinoma were incurred from female patients passing through biopsy or surgery. HPV DNA and genotypes of all subjects were examined using polymerase chain reaction (PCR) and gene-chip. There were 937 positive cases among 1021 cervical squamous cell carcinoma tissue samples with the total HPV infection rate of 91.77%. The HPV infection rate of single type was 75.42%. The predominant types of single infection with HPV were HPV 16 (52.60%), HPV 18 (5.19%), HPV 58 (4.51%), HPV 52 (2.84%), HPV 31 (2.74%), HPV 33 (2.45%), HPV 59 (1.76%) and HPV 45 (0.88%). The HPV infection rate of multiple types was 16.35%. The predominant types of multiple infection with HPV were HPV 16+18 (19.16%), 16+58 (6.59%),16+52 (5.99%), 16+33 (5.39%), 11+16 (2.99%), 16+31 (2.99%), 16+45 (2.99%), 16+42 (2.40%) and 16+59 (2.40%). Cervical squamous cell carcinomas had a close relationship with HPV infection. HPV 16, 18, 58, 52, 31, 33, 59, 45 with high prevalence in the cervical squamous cell carcinoma deserve great attention in eastern China (mainly in Jiangsu Province). The protection spectrum of divalent vaccine and ninevalent vaccine were, respectively, 68.52% (642/937) and 90.82% (851/937) in the cases of cervical squamous cell carcinomas.

Clinical Relevance of HPV 16/18 Testing Methods in Cervical Squamous Cell Carcinoma

2000 ◽  
Vol 8 (4) ◽  
pp. 300-309 ◽  
Author(s):  
Anton- H. Graf ◽  
Annie L.M. Cheung ◽  
Cornelia Hauser-Kronberger ◽  
Nadia Dandachi ◽  
Raymond R. Tubbs ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Clinical Relevance ◽  
Cervical Squamous Cell Carcinoma ◽  
Testing Methods ◽  
Hpv 16

scholarly journals Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian Patients

2020 ◽  
Vol 14 (1) ◽  
pp. 57-64
Author(s):  
Ashraf I. Khasawneh ◽  
Nisreen Himsawi ◽  
Jumana Abu-Raideh ◽  
Muna Salameh ◽  
Niveen Abdullah ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Prophylactic Measures

Background: In addition to smoking and alcohol consumption, human papillomavirus (HPV) is a leading etiology for Head and Neck Squamous Cell Carcinoma (HNSCC). However, this causal association is still understudied in Middle Eastern populations. Objective: The aim of this study was to determine the prevalence of HPV-associated infection in the Jordanian HNSCC patients and the associated HPV genotypes. Methods: Formalin-Fixed Paraffin-Embedded (FFPE) squamous cell carcinoma samples of the head and neck were collected from two referral centers in Amman, Jordan to determine the existence of HPV DNA. After DNA extraction HPV infection and genotyping were identified using real-time PCR. Results: HPV DNA was detected in 19 out of 61 (31.1%) HNSCC samples. Despite screening for 28 different genotypes, HPV 16 was the only genotype identified in all examined samples. Most HPV-positive samples were obtained from the oropharynx (41.7%), oral cavity (37%), and larynx (18.2%). No significant association between HPV 16 genotype and age, sex, tobacco use, anatomical location, or tumor grade was noticed. Conclusion: This study reported a high association between HPV 16 genotype and HNSCC in Jordanian patients. These data should facilitate the implementation of appropriate HPV awareness campaigns, and activate selective prophylactic measures against HPV infection.

scholarly journals Screening History and Diagnostic Characteristics of Cervical Adenocarcinoma: a Retrospective Study

2020 ◽  
Author(s):  
Jing Li ◽  
Minjuan Ye ◽  
Huixia Ye ◽  
Junxian He ◽  
Xiaomao Li ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cervical Screening ◽  
Participation Rate ◽  
Cervical Squamous Cell Carcinoma ◽  
Hpv Infection ◽  
Cervical Adenocarcinoma ◽  
Diagnostic Characteristics ◽  
Screening History

Abstract Background: An increasing trend in incidence of cervical adenocarcinoma has been observed in recent years. This research aims to study the screening history and diagnostic characteristics of cervical adenocarcinoma.Methods: Cervical cancer patients hospitalized in the Gynecology Department of the Third Affiliated Hospital of Sun Yat-sen University between 1st Jan 2017 and 31st Mar 2020 were included. Cervical screening history investigation and analysis were carried out by medical record data inquiry and case investigation.Results: (1)The chief complaint of 72.0% of cervical squamous cell carcinoma was vaginal bleeding. 75.6% of adenocarcinoma came in for abnormal vaginal discharge, p<0.001. (2)The HPV infection rate in adenocarcinoma(74.2%) was lower than that in squamous cell carcinoma(92.9%), p<0.001. (3)The participation rate of cervical screening before diagnosis of adenocarcinoma(21.2%) was higher than that of squamous cell carcinoma(2.8%), p＜0.01. (4) The proportion of early-stage in adenocarcinoma (46.3%) was larger than in SCC (28.3%), p<0.01. Conclusion: Compared to cervical squamous cell carcinoma, HPV infection was found to be less closely associated with adenocarcinoma of uterine cervix. The participation rate and frequency of cervical screening of patients with cervical adenocarcinom were more than squamous cell carcinoma. The screening methods at present may not be sensitive enough for precancerous lesion of adenocarcinoma, but regular and multiple screening are still of great significance for early diagnosis of adenocarcinoma of uterine cervix.

Clinical Relevance of HPV 16/18 Testing Methods in Cervical Squamous Cell Carcinoma

2000 ◽  
Vol 8 (4) ◽  
pp. 300-309
Author(s):  
Anton- H. Graf ◽  
Annie L.M. Cheung ◽  
Cornelia Hauser-Kronberger ◽  
Nadia Dandachi ◽  
Raymond R. Tubbs ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Clinical Relevance ◽  
Cervical Squamous Cell Carcinoma ◽  
Testing Methods ◽  
Hpv 16

scholarly journals Role of HPV-16 in Pathogenesis of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma and Correlation of p16INK4A Expression in HPV-16 Positive Cases: An Immunohistochemical Study

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Gaurav Pralhad Agrawal ◽  
Priya Shirish Joshi ◽  
Anshita Agrawal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Hpv 16 ◽  
Oral Epithelial Dysplasia ◽  
Tissue Sections ◽  
Oral Epithelial

The objective of current study is to evaluate the role of HPV-16 in the pathogenesis of oral epithelial dysplasias (OED) and oral squamous cell carcinoma (OSCC) by immunohistochemistry (IHC) and to know whether HPV-16 participates in disruption of the regulation of p16 INK4A suppressor protein in OED and OSCC by IHC. Histopathologically diagnosed 20 cases of OED and 20 cases of OSCC were selected from amongst the patients attending the OPD of Vasantdada Patil Dental College and Hospital, Sangli. Biopsy tissue section were then tested for HPV-16 by IHC. HPV-16 positive tissue sections were then again tested by p16 by IHC. Overall 22.5% of cases in our study were found to be positive for HPV 16 which includes 10% of cases of OED and 35% cases of OSCC. Amongst the HPV 16 positive cases, more than 60% of cells were positive for p16INK4A IHC in OED (50%) and OSCC (85.71%). Thus, HPV 16 participates in disruption of the regulation of p16INK4A suppressor protein and can be used as surrogate biomarker for detection of HPV infection in OED and OSCC.

scholarly journals Genetic aspects of HPV infection detection in tumor and adjacent tissues of patients with laryngeal squamous cell carcinoma

2016 ◽  
Vol 61 (6) ◽  
pp. 275-279
Author(s):  
O. Yu. Dvoryaninova ◽  
E. G. Nikitina ◽  
V. A. Bychkov ◽  
N. V. Litviakov
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Hpv Infection ◽  
Detection Methods ◽  
Quantitative Detection ◽  
Tissue Samples ◽  
Hpv Dna ◽  
Advantages And Disadvantages

The article describes methods for the human papillomavirus (HPV) detection in tumor and adjacent (morphologically intact) tissues of patients with laryngeal squamous cell carcinoma (LSSC) in terms of viral pathogenesis. Comparative evaluation of the principles and techniques for HPV detection was performed. Advantages and disadvantages of the HPV detection methods are described. Approaches for DNA and HPV oncoproteins E6-E7 identification are substantiated. The results of our research into the qualitative and quantitative detection of HPV in the tumor and adjacent tissues of patients with Lssc are described. The research was conducted using commercial test systems Amplisens HPV HR screen-titre-FL and Amplisens HPV HR genotype-FL. Based on these results we developed the algorithm of HPV detection in samples of tumor tissue of patients with Lssc. The need for typing HPV-positive tissue samples with low concentration of HPV DNA was discussed.

scholarly journals HPV Infection in Esophageal Squamous Cell Carcinoma and Its Relationship to the Prognosis of Patients in Northern China

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Fangli Cao ◽  
Hui Han ◽  
Fang Zhang ◽  
Baozhong Wang ◽  
Wei Ma ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Hpv Infection ◽  
Cox Proportional Hazards Model ◽  
Rank Test ◽  
Hpv 16 ◽  
Risk Of Death ◽  
Hpv Status

Purpose. Human papillomavirus (HPV) as a risk factor for esophageal squamous cell carcinoma (ESCC) has previously been studied, but importance of HPV status in ESCC for prognosis is less clear.Methods. A total of 105 specimens with ESCC were tested by in situ hybridization for HPV 16/18 and immunohistochemistry for p16 expression. The 5-year overall survival (OS) and progression-free survival were calculated in relation to these markers and the Cox proportional hazards model was used to determine the hazard ratio (HR) of variables in univariate and multivariate analysis.Results. HPV was detected in 27.6% (29) of the 105 patients with ESCC, and all positive cases were HPV-16. Twenty-five (86.2%) of the 29 HPV-positive tumors were stained positive for p16. HPV infected patients had better 5-year rates of OS (65.9% versus 43.4% among patients with HPV-negative tumors;P= 0.002 by the log-rank test) and had a 63% reduction in the risk of death (adjusted HR = 0.37, 95% CI = 0.16 to 0.82, andP= 0.01).Conclusions. HPV infection may be one of many factors contributing to the development of ESCC and tumor HPV status is an independent prognostic factor for survival among patients with ESCC.

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