scholarly journals A COMPARATIVE STUDY OF RED CELL VOLUMES IN HUMAN SUBJECTS WITH RADIOACTIVE PHOSPHORUS TAGGED RED CELLS AND T-1824 DYE 12

1950 ◽  
Vol 29 (2) ◽  
pp. 258-264 ◽  
Author(s):  
Herman M. Nachman ◽  
G. Watson James ◽  
John W. Moore ◽  
Everett Idris Evans ◽  
Evelyn Hayes ◽  
...  
Keyword(s):  
Comparative Study ◽  
Human Subjects ◽  
Red Cells ◽  
Red Cell ◽  
Radioactive Phosphorus ◽  
Cell Volumes

scholarly journals Determinants of Erythrocyte Size in Chronic Liver Disease

Blood ◽  
1969 ◽  
Vol 34 (6) ◽  
pp. 739-746 ◽  
Author(s):  
THOMAS M. KILBRIDGE ◽  
PAUL HELLER
Keyword(s):  
Bone Marrow ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
Peripheral Blood ◽  
Hepatic Cirrhosis ◽  
Alcoholic Cirrhosis ◽  
Clinical Findings ◽  
Red Cells ◽  
Red Cell ◽  
Cell Volumes

Abstract Serial determinations of red cell volumes were made with an electronic sizing device in 30 patients with hepatic cirrhosis. Variations in red cell volumes were correlated with other hematologic and clinical findings. The results of these studies suggest that volume macrocytosis in patients with alcoholic cirrhosis is either due to megaloblastosis of the bone marrow or to an accelerated influx of young red cells into the peripheral blood.

Effect of Hemorrhage on Red Cell and Plasma Volume of Various Organs of the Dog

1958 ◽  
Vol 195 (1) ◽  
pp. 69-72 ◽  
Author(s):  
J. R. Dunn ◽  
S. Deavers ◽  
R. A. Huggins ◽  
E. L. Smith
Keyword(s):  
Protein Content ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cells ◽  
Red Cell ◽  
Well Control ◽  
Volume Calculations ◽  
Initial Blood ◽  
Cell Volumes

Dogs were anesthetized with a morphine-pentobarbital combination. Red cell volumes were measured by radioactive Cr51 and plasma volumes with radioactive I131. The dogs were killed by bleeding and the organs were digested. Aliquot samples of the organs were counted. Twenty minutes after a hemorrhage averaging 46% of the initial blood volume, 7.0 cc/kg of fluid with a mean protein content of 3.2 gm/100 cc entered the circulation. There were 6.7 cc/kg of fluid lost from the organs with 67% of the total coming from the stomach, intestine and skin. After the hemorrhage 2.4 cc/kg of red cells were trapped according to the blood volume calculations, but the cells were not recovered from the organs. The control dogs had 14.1 cc/kg of residual (‘extra’) plasma and after hemorrhage 7.3 cc/kg from the blood volume calculations. Calculation of residual plasma from the organ data agreed well (control 13.8 cc/kg and after hemorrhage 6.1 cc/kg) with the blood volume calculations. Residual plasma remained essentially the same percentage of the blood volume in control and bled dogs.

scholarly journals The Effect of Enhanced Fibrinolytic Activity on the Red Cell Membrane Transport

1977 ◽  
Author(s):  
S.B. Ulutin ◽  
N. B. Emekli ◽  
T. U. Yardimici
Keyword(s):  
Active Transport ◽  
Fibrinolytic Activity ◽  
Time Course ◽  
Degradation Products ◽  
Human Subjects ◽  
Red Cells ◽  
Fibrinolytic System ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Before And After

In dogs and in human subjects, using hepatic vein catheterization before and after the activation of the fibrinolytic system, the blood samples were obtained and the red cell amino acid transport was investigated. The time course accumulation of radioactive histidine in isolated red cells was followed together with the measurements of the fibrinolytic activity.A decrease in the active transport of histidine was observed in the red cells after the stimulation of the fibrinolytic system. Also a correlation between the decrease of active transport and the increase of fibrin-fibrinogen degradation products was seen.

scholarly journals On the Distribution of Red Cell Volumes

Blood ◽  
1968 ◽  
Vol 31 (4) ◽  
pp. 503-515 ◽  
Author(s):  
BRIAN S. BULL
Keyword(s):  
Red Cells ◽  
Volume Distribution ◽  
Red Cell ◽  
Sodium Potassium ◽  
Normal Distributions ◽  
Potassium Tartrate ◽  
Particle Population ◽  
Cell Subpopulations ◽  
Log Normal ◽  
Cell Volumes

Abstract A large number of red cell volume distribution curves can be generated on the same sample of blood by manipulating the length of the Coulter Counter aperture and the shape of the red cell. Curves derived from sphered red cells flowing through an aperture 70 x 98µ long, or longer appear to be relatively free from artefacts and therefore the most likely to be "real." The use of long apertures to size sphered red cells will give a volume distribution curve that is approximately Gaussian and with a mean that is large relative to the variance. Whether red cells are Gaussian or log normal in their volume distribution is very difficult to determine. On a particle population where volumes are controlled as closely as on red cells (i.e. with such a low variance), the normal and the log normal distributions become practically indistinguishable. If in the future this decision can be reached by some independent means, then the volume distributions using the Coulter volume transducer can be easily and simply fitted to the shape desired by appropriate manipulation of aperture length. These ideal volume distribution curves are not likely to differ markedly from those obtained under the conditions suggested in this paper. For the present, use of a 70 x 98µ aperture to size red cells sphered by suspension in isotonic Sodium Potassium Tartrate will contribute to considerably greater accuracy in identification of red cell subpopulations in abnormal blood.

scholarly journals Multiple red cell volumes (RCV) can be independently determined in sheep in vivo using red cells (RBCs) labeled at 4 biotin densities

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Donald M Mock ◽  
Nell I Matthews ◽  
Ronald G Strauss ◽  
Leon F Burmeister ◽  
Robert Schmidt ◽  
...  
Keyword(s):  
Red Cells ◽  
Red Cell ◽  
Cell Volumes

Measurement of rapidly and slowly circulating red cell volumes in hemorrhagic shock

1962 ◽  
Vol 202 (6) ◽  
pp. 1179-1182 ◽  
Author(s):  
William C. Shoemaker
Keyword(s):  
Cell Volume ◽  
Red Cells ◽  
Blue Dye ◽  
Red Cell ◽  
Compartment System ◽  
Red Cell Volume ◽  
Circulating Cells ◽  
Final Volume ◽  
The Difference ◽  
Cell Volumes

Equilibration of injected Cr51-labeled red cells and Evan's blue dye (T-1824) in dogs occurred usually within 10 min in control conditions. Delayed equilibration of labeled red cells but not T-1824 was observed after hemorrhage and retransfusion of the withdrawn blood. Delayed equilibration of injected labeled red cells may be not only evidence for the presence of a slowly circulating red cell volume, but also may be used as a measurement of this volume. If a two-compartment system is assumed, then the initial volume of dilution of labeled red cells represents a relatively uniform mixing within a volume of rapidly circulating cells. The final volume of dilution of labeled red cells, beyond which no further dilution occurs, is assumed to represent total circulating red cell volume. The difference between these two volumes may represent a slowly circulating red cell volume. It is postulated that the so-called "sequestered" blood volume may, in part, be reflected in this slowly moving red cell volume. If so, the proposed approach under certain conditions may provide an index to its quantitation.

scholarly journals DIFFRACTOMETRIC MEASUREMENTS OF THE TONICITY-VOLUME RELATIONS OF HUMAN RED CELLS IN HYPOTONIC SYSTEMS

1951 ◽  
Vol 34 (5) ◽  
pp. 567-571 ◽  
Author(s):  
Eric Ponder
Keyword(s):  
Strong Evidence ◽  
Linear Relation ◽  
High Speed ◽  
Diffraction Method ◽  
Red Cells ◽  
Red Cell ◽  
Comparison Of The Results ◽  
Cell Volumes ◽  
Human Red Cells

Red cell volumes in media sufficiently hypotonic to produce partial hemolysis have been measured with a high speed hematocrit and by an improved diffraction method. A comparison of the results shows that the upward departures from the linear relation based on the van't Hoff-Mariotte law are not observed when the volumes are measured diffractometrically. Downward departures are observed at low tonicities by both methods. These results provide strong evidence that the upward departures are due to the inclusion of semirigid ghosts in the column of packed red cells which is measured when the volumes are found with the hematocrit.

Dose-response relationships of human urinary erythropoietin

1959 ◽  
Vol 14 (2) ◽  
pp. 233-236 ◽  
Author(s):  
Joseph F. Garcia ◽  
Donald C. Van Dyke
Keyword(s):  
Dose Response ◽  
Red Cells ◽  
Red Cell ◽  
Simulated Altitude ◽  
Incorporation Assay ◽  
Cell Volumes ◽  
Fasted Rats

The response to graded doses of human urinary erythropoietin was determined in normal, hypophysectomized and fasted rats by use of the Fe59 red cell incorporation assay. In all three cases a highly significant correlation between the red cell incorporation of radioiron and the dose of erythropoietin administered was observed. Also, total red cell volumes and total circulating hemoglobins determined after 14 daily injections of erythropoietin to normal rats showed the presence of polycythemia. At the highest dose administered, the degree of polycythemia was similar to that obtained in rats exposed to a simulated altitude of 20,000 feet for 14 days. The value of the Fe59 red cell incorporation assay as an assay for erythropoietin was substantiated by the high correlation observed between the increase in total circulating hemoglobin and radioiron incorporation into red cells. Submitted on August 18, 1958

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