Stimulation of the proliferation of human bone cells in vitro by human monocyte products with interleukin-1 activity.

Kassem M, Mosekilde L, Eriksen EF. Effects of fluoride on human bone cells in vitro: differences in responsiveness between stromal osteoblast precursors and mature osteoblasts. Eur J Endocrinol 1994;130:381–6. ISSN 0804–4643 The cellular effects of sodium fluoride (NaF) on human bone cells in vitro have been variable and dependent on the culture system used. Variability could be attributed to differences in responsiveness to NaF among different populations of cells at various stages of differentiation in the osteoblastic lineage. In this study we compared the effects of NaF in serum-free medium on cultures of more differentiated human osteoblast-like (hOB) cells derived from trabecular bone explants and on osteoblast committed precursors derived from human bone marrow, i.e. human marrow stromal osteoblast-like (hMS(OB)) cells. Sodium fluoride (10−5 mol/l) increased proliferation of hMS(OB) cells (p<0.05, N = 10) but was not mitogenic to hOB cells (p>0.05, N= 10). Alkaline phosphatase (AP) production increased in both hMS(OB) (p<0.05, N=9) and hOB cells (p<0.05, N=9). No significant effects on procollagen type I propeptide production were obtained in either culture. In the presence of 1,25-dihydroxycholecalciferol (10−9 mol/l), NaF enhanced alkaline phosphatase (p<0.05, N=8), procollagen type I propeptide (p<0.05, N=7) and osteocalcin (p<0.05, N=7) production by hMS(OB) cells but not by hOB cells. Our results suggest that osteoblast precursors are more sensitive to NaF action than mature osteoblasts and that the in vivo effects of NaF on bone formation may be mediated by stimulating proliferation and differentiation of committed osteoblast precursors in bone marrow. M Kassem, Mayo Clinic, Endocrine Research Unit, W-Joseph 5-164, Rochester, MN 55904, USA

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Devices and Techniques for in Vitro Mechanical Stimulation of Bone Cells

Bone Mechanics Handbook ◽

10.1201/b14263-35 ◽

2001 ◽

pp. 723-744 ◽

Cited By ~ 1

Keyword(s):

Mechanical Stimulation ◽

Bone Cells ◽

Stimulation Of

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Stimulation of human monocyte and polymorphonuclear cell iodination and interleukin-1 production by epigallocatechin gallate

Journal of Leukocyte Biology ◽

10.1002/jlb.51.5.478 ◽

1992 ◽

Vol 51 (5) ◽

pp. 478-483 ◽

Cited By ~ 17

Author(s):

Hiroshi Sakagami ◽

Kazuhito Asano ◽

Yukihiko Hara ◽

Tadakatsu Shimamura

Keyword(s):

Interleukin 1 ◽

Epigallocatechin Gallate ◽

Human Monocyte ◽

Polymorphonuclear Cell ◽

Stimulation Of

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Cytokine production by primary bone marrow megakaryocytes

Blood ◽

10.1182/blood.v84.12.4151.bloodjournal84124151 ◽

1994 ◽

Vol 84 (12) ◽

pp. 4151-4156 ◽

Cited By ~ 56

Author(s):

S Jiang ◽

JD Levine ◽

Y Fu ◽

B Deng ◽

R London ◽

...

Keyword(s):

Bone Marrow ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Interleukin 1 ◽

Human Bone ◽

Human Bone Marrow ◽

Tnf Alpha ◽

Necrosis Factor Alpha ◽

Gm Csf

Primary human bone marrow megakaryocytes were studied for their ability to express and release cytokines potentially relevant to their proliferation and/or differentiation. The purity of the bone marrow megakaryocytes was assessed by morphologic and immunocytochemical criteria. Unstimulated marrow megakaryocytes constitutively expressed genes for interleukin-1 beta (IL-1 beta), IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-alpha (TNF-alpha), by the polymerase chain reaction (PCR) and Northern blot analysis. At the protein level, megakaryocytes secreted significant amounts of IL-1 beta (53.6 +/- 3.6 pg/mL), IL-6 (57.6 +/- 15.6 pg/mL), and GM-CSF (24 +/- 4 pg/mL) but not TNF-alpha. Exposure of human marrow megakaryocytes to IL-1 beta increased the levels of IL-6 (87.3 +/- 2.3 pg/mL) detected in the culture supernatants. Transforming growth factor- beta was also able to stimulate IL-6, IL-1 beta, and GM-CSF secretion, but was less potent than stimulation with phorbol-12-myristate-13- acetate (PMA). The secreted cytokines acted additively to maintain and increase the number of colony-forming unit-megakaryocytes colonies (approximately 35%). These studies demonstrate the production of multiple cytokines by isolated human bone marrow megakaryocytes constitutively or stimulated in vitro. The capacity of human megakaryocytes to synthesize several cytokines known to modulate hematopoietic cells supports the concept that there may be an autocrine mechanism operative in the regulation of megakaryocytopoiesis.

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Human bone cells in culture: application to the study of the biocompatibility in vitro

Bone ◽

10.1016/8756-3282(96)87939-7 ◽

1995 ◽

Vol 17 (6) ◽

pp. 596 ◽

Cited By ~ 1

Author(s):

A. Zarrinpourt ◽

Y. Josset ◽

N. . Lalun ◽

G. Hirnber ◽

J.J. Adnet ◽

...

Keyword(s):

Bone Cells ◽

Human Bone ◽

Cells In Culture

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Endothelin-1 Stimulates Monocytesin vitroto Release Chemotactic Activity Identified as Interleukin-8 and Monocyte Chemotactic Protein-1

Mediators of Inflammation ◽

10.1155/s0962935194000207 ◽

1994 ◽

Vol 3 (2) ◽

pp. 155-160 ◽

Cited By ~ 23

Author(s):

E. Helset ◽

T. Sildnes ◽

Z. S. Konopski

Keyword(s):

Human Monocyte ◽

Chemotactic Activity ◽

Dependent Manner ◽

Lipoxygenase Pathway ◽

Lipoxygenase Inhibitors ◽

Endothelin 1 ◽

Monocyte Chemotactic Protein ◽

Chemotactic Protein ◽

Stimulation Of

In the present study we examined whether endothelin-1 stimulation of human monocytes causes release of chemotactic factors. It was found that monocytes released neutrophil- and monocyte-chemotactic activity in a dose- and time-dependent manner in response to ET-1. ET-1 did not show any chemotactic activity by itself. NCA was detected in monocyte supernatants in response to ET-1 (0.01–100 nM) after 1, 4, 8 and 24 h stimulation. MCA was detected only after 24 h stimulation with ET-1 (0.1–100 nM). Preincubation of the monocyte cultures with the lipoxygenase inhibitors nordihydroguaiaretic acid (10−4M) or diethylcarbamazine (10−9M) completely abolished the appearance of NCA and MCA. NCA was neutralized by > 75% using a polyclonal antibody against human interleuktn-8. The ET-1 induced release of IL-8 was confirmed by IL-8 ELISA. A monoclonal antibody against human monocyte chemotactic protein-1 neutralized MCA by > 80%. It is concluded that ET-1 stimulation of monocytesin vitrocauses release of neutrophil- and monocyte-chemotactic activity identified as IL-8 and MCP-I respectively. An intact lipoxygenase pathway is crucial for this effect of ET-1 to occur.

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Defective stimulation of proliferation and collagen biosynthesis of human bone cells by serum from diabetic patients

Acta Endocrinologica ◽

10.1530/acta.0.1270509 ◽

1992 ◽

Vol 127 (6) ◽

pp. 509-514 ◽

Cited By ~ 20

Author(s):

Rolf E Brenner ◽

Bert Riemenschneider ◽

Werner Blum ◽

Martin Mörike ◽

Walter M Teller ◽

...

Keyword(s):

Metabolic Control ◽

Collagen Synthesis ◽

Bone Cells ◽

Cell Attachment ◽

Cell Number ◽

Human Bone ◽

Diabetic Patients ◽

Inhibitory Influence ◽

Control Serum

We studied the influence of fasting serum from nine insulin-dependent diabetic children and adolescents under insufficient metabolic control on normal human bone cells in vitro compared with serum from eight sex- and age-matched controls. Cell number 24 h after plating was significantly less under diabetic serum, indicating impaired cell attachment, spreading and initiation of cell proliferation. Cell number after five days was reduced by 1% diabetic serum, while higher serum concentrations had diverging effects on osteoblast proliferation. Collagen synthesis of human osteoblasts was significantly reduced by 8% diabetic serum compared to 8% control serum, while synthesis of non-collagenous proteins was not affected. Duration of diabetes (several weeks up to 12 years) had no influence on these parameters. The serum from one patient, which was studied a second time under excellent metabolic control three months later, however, had lost its inhibitory influence on collagen synthesis of osteoblasts. The pattern of the interstitial collagen types I, III and V was not altered by diabetic serum. These results indicate that defective regulation of proliferation and collagen synthesis of osteoblasts by components present in human diabetic serum may be an important factor in the development of diabetic osteopenia. The negative influence might be explained in part by reduced levels of IGF-I and elevated levels of IGF binding protein-1 in the diabetic sera.

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