Correlation of Nasal Fluid Biomarkers and Symptoms in Patients with Persistent Allergic Rhinitis

2020 ◽  
Vol 129 (6) ◽  
pp. 542-547
Author(s):  
Su Il Kim ◽  
Oh Eun Kwon ◽  
Jung Min Park ◽  
Jeon Gang Doo ◽  
Seok Hyun Kim ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Inverse Correlation ◽  
Lavage Fluid ◽  
Nasal Symptom ◽  
Clara Cell ◽  
Cell Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Prick Test ◽  
Persistent Allergic Rhinitis ◽  
Nasal Fluid

Objectives: This study investigated whether the biomarkers present in nasal fluid reflect the severity of symptoms in patients with persistent allergic rhinitis (PAR). Methods: We enrolled 29 PAR patients complaining of nasal symptoms and testing positive to skin prick test. Patients’ total nasal symptom score (TNSS) was measured and their nasal lavage fluid (NALF) was collected. The levels of biomarkers including Clara cell protein 16 (CC16), tryptase, and interleukin 5 (IL-5) in NALF were determined via enzyme-linked immunosorbent assay (ELISA). Results: PAR patients were classified into persistent mild and persistent moderate-to-severe groups according to the Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines. The CC16 alone was significantly negatively correlated with TNSS ( P < .05). Further, the CC16 level was significantly lower in persistent moderate-to-severe group than persistent mild group of patients ( P < .05). Conclusions: The levels of CC16 alone among several NALF biomarkers showed an inverse correlation with symptoms of PAR patients.

scholarly journals Eosinophil Chemokines and Clara Cell Protein 16 Production in Nasal Mucosa of Patients with Persistent Allergic Rhinitis

2017 ◽  
Vol 49 (3) ◽  
pp. 178-182 ◽  
Author(s):  
Aleksandar Peric ◽  
Cveta Spadijer Mirkovic ◽  
Biserka Vukomanovic Durdevic ◽  
Aneta V. Peric ◽  
Danilo Vojvodic
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Clara Cell ◽  
Cell Protein ◽  
Clara Cell Protein ◽  
Persistent Allergic Rhinitis

Measurement of Tryptase and CC16/Albumin in Nasal Lavage Fluid as a Screening Tool of Allergic Rhinitis

2021 ◽  
pp. 194589242199826
Author(s):  
Oh Eun Kwon ◽  
Young Chan Lee ◽  
Jung Min Park ◽  
Sung Wan Kim ◽  
Young-Gyu Eun ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Diagnostic Tool ◽  
Screening Tool ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Cell Protein ◽  
Diagnostic Tools ◽  
Enzyme Linked Immunosorbent Assay ◽  
Albumin Ratio ◽  
Nasal Lavage Fluid

Background There is no trial to make a diagnostic tool of allergic rhinitis (AR) utilizing biomarkers from nasal fluid. Base on previous studies, we selected following five biomarkers in nasal fluids that represent the characteristics of allergic reactions: tryptase, eosinophil cationic protein (ECP), interleukin 5 (IL-5), Clara cell protein 16 (CC16) and CC16-to-albumin ratio. Objective This study aimed to identify biomarkers in nasal discharge that may be used in biosensors to diagnose AR as an additional diagnostic tool. Methods Patients showed rhinorrhea and tested positive on allergic skin and specific immunoglobulin E (IgE) tests were included in the AR group. The non-AR group included individuals no dominant nasal symptoms and tested negative on allergy tests. Nasal lavage fluid samples were collected from all participants. Biomarkers in the samples were quantified using enzyme-linked immunosorbent assay. Results Forty-five patients with AR and 28 non-AR subjects were enrolled in this study. Comparing the concentrations of biomarkers, the concentrations of tryptase and IL-5 were significantly higher in the AR group than in the NAR group. And CC16 level and CC16-to-albumin ratio were significantly lower in the AR group. In the combination of tryptase or CC16-to-albumin ratio, the sensitivity was 90.7% and the specificity was 64.3% ( p = 0.013). Conclusion The combination of “tryptase or CC16-to-albumin” could be used as a screening tool for AR. Although this diagnostic method could not replace conventional diagnostic tools, we could consider the method we proposed as an additional screening tool for patients who could not undergo allergy tests.

scholarly journals Study of causal aeroallergens in allergic rhinitis

2019 ◽  
Vol 5 (4) ◽  
pp. 916
Author(s):  
Divya Aggarwal ◽  
Abhilash S. ◽  
Sunil Kapur ◽  
Divya Gupta
Keyword(s):  
Allergic Rhinitis ◽  
Skin Prick Test ◽  
Symptom Score ◽  
Eosinophil Count ◽  
Severe Disease ◽  
Nasal Symptom ◽  
Total Serum ◽  
Serum Ige ◽  
Prick Test ◽  
Absolute Eosinophil Count

<p class="abstract"><strong>Background:</strong> The diagnosis of allergic rhinitis requires a detailed history, thorough clinical examination and identification of aeroallergens by skin prick test. The diagnosis cannot be confirmed on the basis of symptoms alone because both allergic and non-allergic rhinitis can present with similar symptoms. The present study was undertaken to identify the common aeroallergens causing allergic rhinitis and to find any relationship of the severity of patient’s symptoms with raised levels of absolute eosinophil count, percentage of eosinophils in the peripheral blood and total serum IgE.</p><p class="abstract"><strong>Methods:</strong> It is cross sectional study was conducted among 100 patients including both the genders attending out-patient department of ENT. The patients were divided into mild, moderate and severe disease categories based on nasal symptom score (NSS) which was calculated taking into account the severity of four symptoms namely sneezing, watery rhinorrhoea, itching sensation in nose and nasal obstruction.  </p><p class="abstract"><strong>Results:</strong> 76% of patients had moderate nasal symptom score and 42% had symptoms of allergic rhinitis for less than 3 years. The prevalence of skin prick test was strongly positive for pollens (46.19%), followed by dust (16.4%), dust mites (15.7%), fungus (9.7%), insects (9.24%) and epithelia (2.77%). But, among 68 aeroallergens, most common offending allergen was D-farinae (30%). There was no significant relationship found to exist between Eosinophilia, Absolute eosinophil count and serum IgE level with that of nasal symptom score.</p><p class="abstract"><strong>Conclusions:</strong> Skin prick test is the gold standard investigation of choice for definitive diagnosis of allergic rhinitis.</p><p class="abstract"> </p>

scholarly journals Honeysuckle extract relieves ovalbumin-induced allergic rhinitis by inhibiting AR-induced inflammation and autoimmunity

2019 ◽  
Vol 39 (7) ◽  
Author(s):  
Bin Lin ◽  
Bijuan Cai ◽  
Huige Wang
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Inflammatory Reaction ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Autoimmune Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Levels ◽  
Nasal Lavage Fluid ◽  
Ifn Γ

Abstract Honeysuckle has antiviral, antioxidative and anti-inflammatory properties. Allergic rhinitis (AR) is induced by immunoglobulin E (IgE)-mediated inflammatory reaction. Our study investigates whether honeysuckle extract (HE) has therapeutic effect on AR. An AR model of mice was established by ovalbumin (OVA). Hematoxylin–Eosin staining was used to assess nasal mucosa damage. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum histamine, IgE and interleukin (IL)-2, IL-4, IL-17 and interferon-γ (IFN-γ) from nasal lavage fluid. Western blot was carried out to analyze the protein level from nasal mucosa tissue. We found that HE not only decreased nasal rubbing and sneezing in AR mice, but also reduced AR-induced damage to nasal mucosa. Moreover, HE lowered the levels of serum IgE and histamine and inhibited IL-4 and IL-17 levels from AR mice but raised IL-2 and IFN-γ levels in AR-induced nasal lavage fluid. Our results also showed that HE elevated the protein levels of forkhead box P3 (Foxp3) and T-box transcription factor (T-bet) in AR-induced nasal mucosa tissue, whereas it inhibited signal transducer and activator of transcription (STAT) 3 and GATA binding protein 3 (GATA-3) protein levels. By regulating AR-induced inflammatory reaction and autoimmune response, HE also relieved OVA-induced AR. Thus, HE could be used as a potential drug to treat AR.

scholarly journals Cytokine profiles in nasal fluid of patients with seasonal or persistent allergic rhinitis

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Katrin König ◽  
Christine Klemens ◽  
Katharina Eder ◽  
Marion San Nicoló ◽  
Sven Becker ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Cytokine Profiles ◽  
Persistent Allergic Rhinitis ◽  
Nasal Fluid

scholarly journals An Evaluation of Oxidative Stress With Thiol/Disulfide Homeostasis in Patients With Persistent Allergic Rhinitis

2020 ◽  
pp. 014556132092633
Author(s):  
Ayse E. Göker ◽  
Maide H. Alagöz ◽  
Tolgar L. Kumral ◽  
Semih Karaketir ◽  
Ayça B. Yilmazer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Allergic Rhinitis ◽  
Prospective Observational Study ◽  
Allergen Exposure ◽  
The Other ◽  
Prick Test ◽  
Total Thiol ◽  
Persistent Allergic Rhinitis ◽  
The Mean ◽  
Sinonasal Symptoms

Background: We evaluated the efficacy of medical treatment on thiol–disulfide balance despite ongoing allergic stimulation. Methods: The research design was a prospective observational study that included 35 persistent allergic rhinitis (AR) patients. All patients who were diagnosed with persistent AR were included. A skin prick test was applied to all patients, and the Sino-nasal Outcome Test-22 was used to evaluate sinonasal symptoms. Thiol/disulfide homeostasis balance parameters were measured using a novel automatic and spectrophotometric method and compared statistically. Serum total thiol (TT), native thiol (SH), disulphide (SS), disulphide/native thiol (SS/SH), disulphide/total thiol (SS/TT), and native thiol/total thiol (SH/TT) ratios were measured after the second month of the treatment. Results: The 35 patients included 20 (58%) females and 15 (42%) males. The mean age of the patients was 33.17 ± 9.9 years. Disulphide, SS/SH, and SS/TT ratios decreased significantly after the treatment ( P < .05), while SH and SH/TT increased significantly ( P < .05). The mean SH measurement increased significantly in the second month ( P = .001), but TT mean measurements showed no difference after the treatment ( P = .058). The mean SS measurements, on the other hand, decreased significantly in the second month ( P = .003). Conclusion: Thiol/disulfide homeostasis may be used as a marker to evaluate the efficacy of persistent AR treatments. After the treatment, the increase in SH levels suggested the decrease in oxidative stress, even though allergen exposure continued.

Time and pressure dependence of transvascular Clara cell protein, albumin, and IgG transport during ventilator-induced lung injury in mice

2004 ◽  
Vol 286 (3) ◽  
pp. L604-L612 ◽  
Author(s):  
Sawako Yoshikawa ◽  
Judy A. King ◽  
Susan D. Reynolds ◽  
Barry R. Stripp ◽  
James C. Parker
Keyword(s):  
Lung Injury ◽  
Molecular Size ◽  
Dry Weight ◽  
Lavage Fluid ◽  
Secretory Protein ◽  
Clara Cell ◽  
Ultrastructural Changes ◽  
Cell Protein ◽  
Ventilation Time ◽  
Clara Cell Secretory Protein

We compared the transport of three proteins with different hydrodynamic radii with ultrastructural changes in lungs of intact mice ventilated at peak inflation pressures (PIP) of 15, 35, 45, and 55 cmH2O for 2 h and PIP of 55 cmH2O for 0.5 and 1 h. After 2 h of ventilation, significant increases were observed in plasma Clara cell secretory protein (1.9 nm radius) at 35 cmH2O PIP and in bronchoalveolar lavage fluid albumin (3.6 nm radius) at 45 cmH2O PIP and IgG (5.6 nm radius) at 55 cmH2O PIP. Increased concentrations of all three proteins and lung wet-to-dry weight ratios were significantly correlated with PIP and ventilation time. Clara cell secretory protein and albumin increased significantly after 0.5 h of 55 cmH2O PIP, but IgG increased only after 2 h. Separation of endothelium or epithelium to form blebs was apparent only in small vessels (15-30 μm diameter) at 45 cmH2O PIP and after 0.5 h at 55 cmH2O PIP but became extensive after 2 h of ventilation at 55 cmH2O PIP. Junctional gaps between cells were rarely observed. Ultrastructural lung injury and protein clearances across the air-blood barrier were related to ventilation time and PIP levels. Protein clearances increased in relation to molecular size, consistent with increasing dimensions and frequency of transmembrane aqueous pathways.

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