scholarly journals Some Physiological Factors Affecting the Binding of Cortisol by Human Plasma Proteins

1977 ◽  
Vol 14 (1-6) ◽  
pp. 35-38 ◽  
Author(s):  
J. D. Few ◽  
J. R. Haspineall
Keyword(s):  
Steady State ◽  
Human Plasma ◽  
Plasma Proteins ◽  
Gel Filtration ◽  
Physiological Factors ◽  
Mean Values ◽  
Factors Affecting ◽  
Free Cortisol ◽  
The Mean

Steady-state gel filtration has been used to study the binding of cortisol to human plasma proteins in vitro. Raising the temperature from 37°C to 41°C results in the mean proportion of free (non-protein-bound) cortisol rising approximately from 7% to 11%. Addition of cortisol to plasma ≡ 275 nmol/l) also increased the proportion of free cortisol by approximately 50%. Cortisone is less strongly bound to plasma proteins than cortisol. The mean values (±S.D.) for five samples were free cortisol 8.4 ± 1.1% and free cortisone 26.0±3.8%.

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

scholarly journals Survey of Phenolic Acids, Flavonoids and In Vitro Antioxidant Potency Between Fig Peels and Pulps: Chemical and Chemometric Approach

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2574
Author(s):  
Lahcen Hssaini ◽  
Francisca Hernandez ◽  
Manuel Viuda-Martos ◽  
Jamal Charafi ◽  
Rachid Razouk ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Radical Scavenging ◽  
Fruit Peel ◽  
Mean Values ◽  
Local Cultivar ◽  
Ic50 Values ◽  
The Mean ◽  
Lipid Peroxidation Inhibition ◽  
Almost All

In the present study, chromatic coordinates, phenolic acids, flavonoids and antioxidant capacity assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS) and lipid peroxidation inhibition capacity (LPIC) essays and their relative IC50 were investigated in 25 fig cultivars growing in Morocco. The aims of this study were to determine (i) the variation in these compounds among light and dark-colored cultivars, (ii) their partitioning between fruit peel and pulp and (iii) to display network connections among these variables. Twelve phenolic compounds (PCs) were isolated in peel extract versus eight in pulp samples. Anthocyanins, mainly cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the predominant compounds in peels, where the mean concentrations were 75.90 ± 18.76 and 77.97 ± 18.95 µg/g dw, respectively. On the other hand, (−)-epicatechin and cyanidin-3-O-rutinoside were the major compounds in the pulp extracts, where the mean values were 5.23 ± 4.03 and 9.01 ± 5.67 µg/g dw, respectively. A two-dimensional hierarchically clustered heatmap was applied to the dataset to explore correlations in the dataset and similarities between cultivars, without dimensionality reduction. Results showed that anthocyanins, particularly pelargonidin-3-O-rutinoside, cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the main contributors to the peels’ free radical scavenging capacity. This capacity was particularly higher in the peel of dark-colored figs compared to the fruit pulp. The local cultivar “INRA 1301” showed the most promising phenolic profile due to its very high levels of almost all detected PCs, especially (−)-epicatechin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, cyanidine-3,5-diglucoside, cyanidine-3-O-rutinoside and pelargonidin-3-O-rutinoside (54.66, 141.08, 35.48, 494.08, 478.66, 12.56 µg/g dw, respectively). Having the darkest figs in the collection (L* = 25.72, c* = 22.09 and h° = 20.99), this cultivar has also combined promising IC50 values, which were of 19.85, 40.58 and 124.78 µg/mL for DPPH, ABTS and LPIC essays, respectively.

scholarly journals In vitro factors affecting the concentration of gamma-LpE (γ-LpE) in human plasma

1998 ◽  
Vol 39 (4) ◽  
pp. 861-872 ◽  
Author(s):  
Larbi Krimbou ◽  
Michel Tremblay ◽  
Hélène Jacques ◽  
Jean Davignon ◽  
Jeffrey S. Cohn
Keyword(s):  
Human Plasma ◽  
Factors Affecting

The binding of the antimalarial arteether to human plasma proteins in-vitro

1992 ◽  
Vol 44 (11) ◽  
pp. 940-942 ◽  
Author(s):  
S. Wanwimolruk ◽  
G. Edwards ◽  
S. A. Ward ◽  
A. M. Breckenridge
Keyword(s):  
Human Plasma ◽  
Plasma Proteins

Physiological and biophysical limits to work in the heat for clothed men and women

1978 ◽  
Vol 44 (6) ◽  
pp. 918-925 ◽  
Author(s):  
E. Kamon ◽  
B. Avellini ◽  
J. Krajewski
Keyword(s):  
Steady State ◽  
Vapor Pressure ◽  
Physiological Responses ◽  
Men And Women ◽  
Mean Values ◽  
Air Temperatures ◽  
Significant Difference ◽  
The Mean ◽  
Evaporative Heat Transfer ◽  
The One

Heat-acclimated, lightly clothed men and women (four of each) walked on a treadmill at 25% and 43% VO2 max, respectively, (M =194 W.m-2), under seven air temperatures (Ta) ranging from 36 to 52 degrees C. Each experiment involved 1 h of fixed and a 2nd h of progressively increasing ambient vapor pressure (Pa). The relative steady state of rectal temperature (Tre), mean skin temperature (Tsk), and heart rate (HR) reached in the 1st h were forced upward during the 2nd h by the rising Pa. The critical air vapor pressure (Pcrit) was identified by the Tre point of inflection for each Ta. One man did not fully reach steady state, but inflection could be determined for his physiological responses. The mean values of all points of inflection were calculated for Tre, Tsk, and HR. Significant sex difference in HR was found only by excluding the results of the one man. Tre and Tsk showed no significant difference between men and women. The coefficient for evaporative heat transfer (he), which could be derived using the Pcrit for the low Ta range, was 14.5 +/- 2.2 W.m-1 Torr-1.

scholarly journals The early changes in retinol-binding protein and prealbumin concentrations in plasma of protein–energy malnourished children after treatment with retinol and an improved diet

1980 ◽  
Vol 43 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Suzanne Large ◽  
G. Neal ◽  
J. Glover ◽  
O. Thanangkul ◽  
R. E. Olson
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Nutritional Status ◽  
Plasma Proteins ◽  
Binding Protein ◽  
Vitamin A Deficiency ◽  
Plasma Concentrations ◽  
Retinol Binding Protein ◽  
Mean Values ◽  
The Mean

1. Changes in total retinol-binding protein (RBP), the holoprotein (holoRBP) and prealbumin (PA) concentrations have been monitored in plasma of thirty protein- and vitamin A-deficient preschool children from within a few hours up to 7 weeks after treatment with retinol and a good-quality protein diet.2. The children were classified into groups according to nutritional status as having either kwashiorkor, marasmus-kwashiorkor or marasmus, and given formula diets whose protein and energy contents increased stepwise from 1 g and 105 kJ/kg body-weight respectively up to 4 g and 733 kJ/kg body-weight after 4 weeks. Retinol was administered in the forms of retinyl palmitate either orally or intramuscularly.3. PA and total RBP were determined by electroimmunoassay procedures and the holoRBP by its fluorescence after separation from other plasma proteins.4. RBP in plasma of the vitamin A-deficient child is largely denatured and incapable of binding administered retinol, which must first be taken up by the liver before native holoRBP is released. An increased pool of native apoprotein accumulates in the liver during vitamin A deficiency which is released into plasma quickly after retinol uptake to form peak concentrations of total and holoRBP approximately 3 h after dosing intramuscularly and 6 h orally.5. The accumulated pool of RBP was highest in livers from the marasmus group and lowest in those from the kwashiorkor group, reflecting their relative capacities to synthesize plasma proteins.6. The mean plasma concentrations of total and holoRBP for the various groups were minimal 24–48 h after dosing with retinol and then improved almost linearly over the following week.7. Mean plasma PA concentrations of the various groups on admission were also in order of the severity of their malnutrition. There was little or no change in this protein concentration over the first 24 h after dosing with retinol, but thereafter the mean values rose almost linearly over 2 weeks. Albumin on the other hand changed little during the first week. The results show that PA is the more sensitive measurement of protein nutritional status.

Factors Affecting Internationalization Degree of Higher Education Institutions in Portugal and Spain

2018 ◽  
pp. 348-377
Author(s):  
Marina Amorim Sousa ◽  
Tomás Bañegil Palacios ◽  
Beatriz Corchuelo Martínez-Azúa
Keyword(s):  
Higher Education ◽  
Data Collection ◽  
Higher Education Institutions ◽  
Organizational Level ◽  
Levels Of Analysis ◽  
Internationalization Process ◽  
Mean Values ◽  
Factors Affecting ◽  
The Mean ◽  
Degree Of Internationalization

The aim of this study is to evaluate the degree of internationalization of Iberian Higher Education Institutions (HEIs) and the factors that influence their internationalization process. The study begins with the contextualization of the HEI internationalization process through a brief historical synthesis and the establishment of the levels of analysis of this process, to focus, in more detail, the organizational level. To this end, it is supported in an organization dimensions model to define the components of the internationalization process and the data collection by questionnaire. The results were processed for each of its components, and the degree of internationalization was obtained by calculating the mean values of the components total. The study concludes that the Iberian HEIs have an interesting level of internationalization, which is higher for institutions with more than 5.000 students, with simultaneous focus on teaching and research, conferring the master's and doctor's degrees.

Biomechanical study of thoracolumbar junction fixation devices with different diameter dual-rod systems

2006 ◽  
Vol 4 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Ung-Kyu Chang ◽  
Jesse Lim ◽  
Daniel H. Kim
Keyword(s):  
Biomechanical Testing ◽  
Load Sharing ◽  
Biomechanical Study ◽  
Compression Force ◽  
Mean Values ◽  
Fixation Devices ◽  
Thoracolumbar Region ◽  
Rod System ◽  
The Mean

Object Advances in the design of a smaller-diameter rod system for use in the thoracolumbar region prompted the authors to undertake this biomechanical study of two different thoracolumbar implants. Methods In vitro biomechanical testing was performed using human cadaveric spines. All specimens were loaded to a maximum moment of 5 Nm with 300-N axial preload in six modes of motion. Two types of anterior implants with different rod diameters were applied to intact T10–12 specimens in two groups. The loading was repeated and the range of motion (ROM) was measured. A T-11 corpectomy was then performed and a strain gauge–mounted carbon fiber stackable cage was implanted. The ROM and compression force on the cage were measured, and the mean values were compared between these two groups. With stabilization of the intact spine, ROM decreased least in extension and greatest in bending compared with the intact specimens. After corpectomy and stabilization, ROM increased in extension by 104.89 ± 53.09% in specimens with a 6.35-mm rod insertion and by 83.81 ± 16.96% in those with a 5.5-mm rod, respectively; in flexion, ROM decreased by 26.98 ± 27.43% (6.35 mm) and by 9.59 ± 15.42% (5.5 mm), respectively; and in bending and rotation, both groups each showed a decrease in ROM. The load sharing of the cage was similar between the two groups (the 6.35-mm compared with 5.5-mm rods): 47.44 and 44.73% (neutral), 49.16 and 39.02% (extension), 61.90 and 56.88% (flexion), respectively. Conclusions There were no statistical differences in the ROM and load sharing of the cage when either the 6.35-or 5.5-mm-diameter dual-rod was used.

Granulokinetics in normal dogs

1964 ◽  
Vol 206 (1) ◽  
pp. 83-88 ◽  
Author(s):  
S. O. Raab ◽  
J. W. Athens ◽  
O. P. Haab ◽  
D. R. Boggs ◽  
H. Ashenbrucker ◽  
...  
Keyword(s):  
Whole Blood ◽  
Turnover Rate ◽  
Half Time ◽  
Total Blood ◽  
Mean Values ◽  
Hexadimethrine Bromide ◽  
The Mean ◽  
Blood Granulocyte ◽  
Number Of Cells

Dog granulocytes were labeled in vitro with radioactive diisopropylfluorophosphate (DFP32) and then returned to the circulation of the donor. Granulocytes were separated from whole blood by utilizing hexadimethrine bromide as the sedimenting agent and saponin as a lysing agent. The labeled granulocytes disappeared from the circulation in an exponential fashion with a mean (±1 sd) half-time disappearance of 5.6 ± 0.95 hr. The size of the total blood granulocyte ( TBGP), circulating granulocyte ( CGP), and marginal granulocyte ( MGP) pools, and the granulocyte turnover rate ( GTR) were measured in 31 normal, unanesthetized dogs. The mean values ± 1 sd, expressed as number of cells x107/kg body wt., were as follows: TBGP, 102 ± 34.8; CGP, 54 ± 20.7; MGP, 48 ± 23.4; and GTR, 305 ± 111.5 cells/kg day. The values observed in anesthetized and in unanesthetized, splenectomized dogs were not significantly different from the above values.

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