scholarly journals Performance of Three Enzymic Methods for Filter Paper Glucose Determination

1984 ◽  
Vol 21 (5) ◽  
pp. 411-416 ◽  
Author(s):  
J M Burrin ◽  
C P Price
Keyword(s):  
Glucose Oxidase ◽  
Filter Paper ◽  
Time Course ◽  
Glucose Dehydrogenase ◽  
Home Monitoring ◽  
Diabetic Patients ◽  
Trichloracetic Acid ◽  
Elution Time ◽  
Glucose Determination ◽  
Blood Spots

Collection of blood spots on filter paper offers a practical alternative for home monitoring of diabetic patients. We have compared the merits of three protein precipitants, trichloracetic acid (TCA), perchloric acid (PCA) and sulphosalicylic acid (SSA) for the elution of glucose from the filter paper, and their subsequent effects on three enzymic methods, glucose dehydrogenase (GDH), hexokinase (HK), and glucose oxidase (GOD) for the determination of glucose using a microcentrifugal analyser. The combination of TCA elutant with the GDH method was superior with respect to time course of reaction and elution time from the filter paper, and was chosen for routine use. Within- and between-batch precision for this method was 2·7% and 3·2% respectively at normal glucose concentrations. Recovery of glucose added to whole blood was 110 ± 5%. Comparison with an automated glucose oxidase method for plasma glucose gave a slope of 1·1, intercept of −0·7 and a correlation coefficient of 0·9 ( n = 64). We conclude that the combination of TCA and glucose dehydrogenase provides a robust, precise and accurate method for the quantitation of glucose in filter-paper blood spots. The procedure offers increased sensitivity and better precision than GOD methods. The use of TCA as elutant gives a faster elution time and has the least effect on any of the enzymic methods.

scholarly journals A Comparison of Three Methods for the Estimation of Capillary Blood Glucose in Filter Paper Spots

1982 ◽  
Vol 19 (1) ◽  
pp. 22-25 ◽  
Author(s):  
R P Taylor ◽  
C A Pennock
Keyword(s):  
Blood Glucose ◽  
Filter Paper ◽  
Trichloroacetic Acid ◽  
Dried Blood Spots ◽  
Capillary Blood ◽  
Glucose Measurement ◽  
Diabetic Patients ◽  
Capillary Blood Glucose ◽  
Blood Spots ◽  
Heavy Workload

A comparison has been made between three methods for measuring capillary blood glucose collected on filter paper, including a new method using a Beckman glucose analyser 2. The methods are suitable for use with capillary blood spots collected onto filter paper for monitoring the control of diabetic patients. Their accuracy, precision, and recovery of glucose were similar. Glucose measurement with the Beckman glucose analyser 2 involves elution of the dried blood spots with 2% trichloroacetic acid and injection of a fraction of the eluate into the analyser. The method provides a simple and rapid alternative procedure for laboratories that do not have a heavy workload for this type of assay.

scholarly journals Glucose Determination by Means of Steady-state and Time-course UV Fluorescence in Free or Immobilized Glucose Oxidase

Sensors ◽  
2007 ◽  
Vol 7 (11) ◽  
pp. 2612-2625 ◽  
Author(s):  
Paola De Luca ◽  
Maria Lepore ◽  
Marianna Portaccio ◽  
Rosario Esposito ◽  
Sergio Rossi ◽  
...  
Keyword(s):  
Steady State ◽  
Glucose Oxidase ◽  
Time Course ◽  
Glucose Determination ◽  
Uv Fluorescence ◽  
Immobilized Glucose Oxidase

Alpha- l -iduronidase and arylsulfatase B in dried blood spots on filter paper: Biochemical parameters and time stability

2017 ◽  
Vol 50 (7-8) ◽  
pp. 431-435 ◽  
Author(s):  
Ana Carolina Breier ◽  
Jaqueline Cé ◽  
Jamila Mezzalira ◽  
Vanessa V. Daitx ◽  
Vitoria C. Moraes ◽  
...  
Keyword(s):  
Filter Paper ◽  
Biochemical Parameters ◽  
Dried Blood Spots ◽  
Time Stability ◽  
Blood Spots ◽  
Arylsulfatase B

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1

Longitudinal study on the use of dried blood spots for home monitoring in children after kidney transplantation

2017 ◽  
Vol 21 (6) ◽  
pp. e12983 ◽  
Author(s):  
A. Al-Uzri ◽  
K. A. Freeman ◽  
Jordan Wade ◽  
K. Clark ◽  
L. A. Bleyle ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Longitudinal Study ◽  
Home Monitoring ◽  
Dried Blood Spots ◽  
Blood Spots

Determination of Chloroquine in Dried Blood Spots on Filter Paper

1986 ◽  
Vol 8 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Y. Bergqvist ◽  
Ö. Ericsson ◽  
M. Rais
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

Upregulation of PKC genes and isozymes in cardiovascular tissues during early stages of experimental diabetes

2003 ◽  
Vol 12 (2) ◽  
pp. 139-146 ◽  
Author(s):  
Mingzhang Guo ◽  
Mack H. Wu ◽  
Ferenc Korompai ◽  
Sarah Y. Yuan
Keyword(s):  
Protein Expression ◽  
Cardiovascular System ◽  
Time Course ◽  
Experimental Diabetes ◽  
Pathological Condition ◽  
Expression Profiles ◽  
Diabetic Patients ◽  
Mrna Levels ◽  
Early Stages ◽  
Protein Expression Profiles

The protein kinase C (PKC) pathway has recently been recognized as an important mechanism in the development of diabetic complications including cardiomyopathy and angiopathy. Although an increase in PKC kinase activity has been detected in the cardiovascular system of diabetic patients and animals, it is unclear whether the same pathological condition alters PKC at the transcriptional and translational levels. In this study we assessed quantitatively the mRNA and protein expression profiles of PKC isozymes in the heart and vascular tissues from streptozotocin-induced diabetic pigs. Partial regions of the porcine PKCα, β1, and β2 mRNAs were sequenced, and real-time RT-PCR assays were developed for PKC mRNA quantification. The results showed a significant increase in the mRNA levels of PKCα, β1, and β2 in the heart at 4–8 wk of diabetes. In concomitance, the PKCβ1 and β2 genes, but not the PKCα gene, were upregulated in the diabetic aorta. Correspondingly, there was a significant increase in the protein expression of PKCα and β2 in the heart and PKCβ2 in the aorta with a time course correlated to that of mRNA expression. In summary, PKCβ2 was significantly upregulated in the heart and aorta at both the transcriptional and translational levels during early stages of experimental diabetes, suggesting that PKCβ2 may be a prominent target of diabetic injury in the cardiovascular system.

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