In Vitro Determination of the Antimicrobial Properties of Two Cyanoacrylate Preparations

1975 ◽  
Vol 54 (3) ◽  
pp. 500-503 ◽  
Author(s):  
Gary N. Blum ◽  
William A. Nolte ◽  
Paul B. Robertson
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Lactobacillus Casei ◽  
Antimicrobial Properties ◽  
Plate Technique ◽  
Inhibition Of Growth ◽  
And Staphylococcus Aureus

Isobutyl and trifluoro cyanoacrylates showed varying degrees of inhibition for Lactobacillus casei and Staphylococcus aureus when tested by the spread plate technique. Candida albicans and Pseudomonas aeruginosa were resistant. The results tend to support the view that inhibition of growth was due to the vapor effect and not the diffusibility of the cyanoacrylates.

Use of internally validated in vitro biofilm models to assess antibiofilm performance of silver-containing gelling fibre dressings

2020 ◽  
Vol 29 (3) ◽  
pp. 154-161 ◽  
Author(s):  
Louise Suleman ◽  
Liam Purcell ◽  
Hannah Thomas ◽  
Samantha Westgate
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Antimicrobial Properties ◽  
Single Species ◽  
In Vitro Models ◽  
Wound Dressings ◽  
In Vitro Test ◽  
Multispecies Biofilms

Objective: To assess the efficacy of five silver-containing gelling fibre wound dressings against single-species and multispecies biofilms using internally validated, UKAS-accredited in vitro test models. Method: Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans single- and multispecies biofilms were cultured using Centres for Disease Control (CDC) biofilm reactors and colony drip flow reactors (CDFR). Following a 72 hour incubation period, the substrates on which biofilms were grown were rinsed to remove planktonic microorganisms and then challenged with fully hydrated silver-containing gelling fibre wound dressings. Following dressing application for 24 or 72 hours, remaining viable organisms from the treated biofilms were quantified. Results: In single-species in vitro models, all five antimicrobial dressings were effective in eradicating Staphylococcus aureus and Pseudomonas aeruginosa biofilm bacteria. However, only one of the five dressings (Hydrofiber technology with combination antibiofilm/antimicrobial technology) was able to eradicate the more tolerant single-species Candida albicans biofilm. In a more complex and stringent CDFR biofilm model, the hydrofiber dressing with combined antibiofilm/antimicrobial technology was the only dressing that was able to eradicate multispecies biofilms such that no viable organisms were recovered. Conclusion: Given the detrimental effects of biofilm on wound healing, stringent in vitro biofilm models are increasingly required to investigate the efficacy of antimicrobial dressings. Using accredited in vitro biofilm models of increasing complexity, differentiation in the performance of dressings with combined antibiofilm/antimicrobial technology against those with antimicrobial properties alone, was demonstrated.

scholarly journals Aktivitas Antimikroba Ekstrak Etanol dan Fraksi Organik Rimpang Wualae (Etlingera elatior (Jack) R.M. Smith)

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Wa Ode Sitti Musnina ◽  
W Wahyuni ◽  
Fadhliyah Malik ◽  
Yusni Oktaviani Timung ◽  
Carla Wulandari Sabandar
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
E Coli ◽  
Plate Technique ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) merupakan tanaman rempah yang digunakan oleh masyarakat sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui metabolit sekunder, aktivitas antibakteri dan aktivitas antijamur ekstrak etanol dan fraksi organik  rimpang wualae. Rimpang wualae diekstraksi dengan metode maserasi menggunakan etanol kemudian di fraksinansi menggunakan pelarut n-heksana, etil asetat, dan metanol. Fraksi n-heksana, etil asetat, dan metanol diuji secara in vitro dengan metode Cup-plate technique, yang dilakukan terhadap bakteri Gram positif Staphylococcus aureus ATCC 25923, Bacillus subtilis FNCC 0060 dan Streptococcus mutans ATCC 2517 dan bakteri Gram negatif Escherichia coli ATCC 35218, Pseudomonas aeruginosa, Salmonella enteric dan jamur Candida albicans ATCC 10231. Kandungan senyawa metabolit sekunder rimpang wualae ditentukan dengan metode skrining fitokima menggunakan perekasi warna. Hasil skrining fitokimia ekstrak etanol fraksi n-heksana, etil asetat, metanol dan etanol rimpang wualae memiliki kandungan alkaloid, flavonoid, saponin, tannin dan terpenoid. Ekstrak etanol mengandung alkaloid, flavonoid, saponin, tannin, dan terpenoid, fraksi n-heksana mengandung terpenoid, fraksi etil asetat mengandung flavonoid, tannin dan terpenoid, fraksi metanol mengandung alkaloid, saponin, tannin, fraksi etanol mengandung flavonoid, saponin, tannin, dan terpenoid.  Hasil pengujian aktivitas antibakteri menunjukkan bahwa ekstrak etanol, fraksi n-heksana, etil asetat, metanol dan etanol rimpang wualae tidak aktif terhadap bakteri S. aureus, B. subtilis, S. mutans, E. coli, P, aeruginosa dan S. enterica pada konsentrasi 100-12,5 mg/mL. Pengujian antijamur menunjukkan bahwa fraksi etil asetat aktif terhadap jamur C. albicans  pada konsentrasi 100, 50, 25, dan 12,5 mg/mL dengan nilai DDH masing-masing sebesar nilai 9,75; 9,5; 8,75; dan 8 mmKata kunci: wualae, Etlingera, antimikroba, obat tradisional, Sulawesi TenggaraJurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tadulako, Jl. Soekarno Hatta Km. 9 Palu

scholarly journals Polyurethane Microstructures-a Good or Bad in vitro Partner for the Isoflavone Genistein?

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Corina Danciu ◽  
Florin Borcan ◽  
Codruta Soica ◽  
Istvan Zupko ◽  
Erzsébet Csányi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Salmonella Enteritidis ◽  
Practical Application ◽  
Preliminary Study ◽  
Micro Structures

In recent years polyurethane microstructures (PM) have gained increasing attention in the pharmaceutical field due to the importance of their practical application. Since finding that such a formulation with genistein could improve its applications, we have conducted a preliminary study regarding the in vitro antiproliferative (MCF7, MDA-MB-231 and T47D) and antimicrobial ( Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis (D), Bacillus subtilis, B. cereus, and Candida albicans) activity in order to test whether polyurethane micro structuresre present a good option for further modulation of genistein's bioavailability. It was concluded that the polyurethane micro structures are a bad in vitro partner for the isoflavone genistein.

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