scholarly journals Heat-killed Salmonella Typhimurium protects mice against carbon ion radiation

2020 ◽  
Vol 48 (10) ◽  
pp. 030006052092425
Author(s):  
Zebin Liao ◽  
Zhe Liu ◽  
Zhenyu Gong ◽  
Xuguang Hu ◽  
Yuanyuan Chen ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Outer Space ◽  
Organ Damage ◽  
Heavy Ion ◽  
Radioprotective Effect ◽  
Carbon Ion ◽  
Multiple Organs ◽  
Ion Accelerator ◽  
Heavy Ion Radiation ◽  
Apoptotic Proteins

Objective Patients receiving carbon-ion radiation therapy and astronauts exploring outer space are inevitably exposed to heavy ion radiation. The aim of this study was to develop radioprotectors to minimize the injuries induced by carbon ion radiation. Methods Heat-killed Salmonella Typhimurium (HKST) was administered to mice by gavage prior to irradiation with a 12C6+ heavy ion accelerator. Hematoxylin and eosin staining and immunofluorescence TdT-mediated dUTP Nick-End Labeling staining were used to assess the radioprotective effect of HKST on organ damage and levels of apoptosis, respectively, in mice. To investigate the mechanism underlying the radioprotective effect of HKST, levels of the pro-apoptotic proteins BAX and caspase 3 as well as interferon-regulatory factor (IRF) 3/7 in the femur, testis and intestine were assessed using immunofluorescence. Results Injuries induced by carbon ion radiation were significantly eased by pretreatment with HKST. Both apoptosis and high expression levels of pro-apoptotic proteins induced by heavy ion radiation were inhibited by HKST pretreatment. The radioprotective effect of HKST was associated with stimulation of Toll-like receptor signaling mediated by enhanced IRF3 and IRF7 signaling. Conclusion HKST was an effective radioprotector alleviating damage to multiple organs caused by heavy ion radiation.

scholarly journals Akt Inhibition Enhanced the Growth Inhibition Effects of Low-Dose Heavy-Ion Radiation via the PI3K/Akt/p53 Signaling Pathway in C6 Glioblastoma Cells

2021 ◽  
Vol 11 ◽  
Author(s):  
Ke Huang ◽  
Wei Zhao ◽  
Xuqiao Wang ◽  
Yingfei Qiu ◽  
Zelin Liu ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Signaling Pathway ◽  
Low Dose ◽  
Ion Irradiation ◽  
Heavy Ion ◽  
C6 Cells ◽  
Carbon Ion ◽  
P53 Signaling ◽  
Heavy Ion Radiation ◽  
P53 Signaling Pathway

BackgroundGlioma has one of the highest mortality rates of all tumors of the nervous system and commonly used treatments almost always fail to achieve tumor control. Low-dose carbon-ion radiation can effectively target cancer and tumor cells, but the mechanisms of growth inhibition induced by heavy-ion radiation via the PI3K/Akt signaling pathway are unknown, and inhibition by heavy-ion radiation is minor in C6 cells.MethodsCarbon-ion radiation was used to investigate the effects of heavy-ion radiation on C6 cells, and suppression of Akt was performed using perifosine. MTT assays were used to investigate optimal perifosine treatment concentrations. Clone formation assays were used to investigate the growth inhibition effects of carbon-ion radiation and the effects of radiation with Akt inhibition. Lactate dehydrogenase release, superoxide dismutase activity, and malondialdehyde content were assessed to investigate oxidative stress levels. Expression levels of proteins in the PI3K/Akt/p53 signaling pathway were assessed via western blotting.ResultsThe 10% maximum inhibitory concentration of perifosine was 19.95 μM. In clone formation assays there was no significant inhibition of cell growth after treatment with heavy-ion irradiation, whereas perifosine enhanced inhibition. Heavy-ion radiation induced lactate dehydrogenase release, increased the level of malondialdehyde, and reduced superoxide dismutase activity. Akt inhibition promoted these processes. Heavy-ion radiation treatment downregulated Akt expression, and upregulated B-cell lymphoma-2 (Bcl-2) expression. p53 and Bcl-2 expression were significantly upregulated, and Bcl-2-associated X protein (Bax) expression was downregulated. The expression profiles of pAkt, Bcl-2, and Bax were reversed by perifosine treatment. Caspase 3 expression was upregulated in all radiation groups.ConclusionsThe growth inhibition effects of low-dose heavy-ion irradiation were not substantial in C6 cells, and Akt inhibition induced by perifosine enhanced the growth inhibition effects via proliferation inhibition, apoptosis, and oxidative stress. Akt inhibition enhanced the effects of heavy-ion radiation, and the PI3K/Akt/p53 signaling pathway may be a critical component involved in the process.

scholarly journals Inhibitory Effect of Ionizing Radiation on Echinococcus granulosus Hydatid Cyst

Diseases ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 23
Author(s):  
Yumin Zhao ◽  
Weifeng Gui ◽  
Yishu Zhang ◽  
Gang Mo ◽  
Dayu Li ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Ionizing Radiation ◽  
Hydatid Cyst ◽  
Biological Effects ◽  
Heavy Ion ◽  
Hydatid Cysts ◽  
Carbon Ion ◽  
X Ray ◽  
Heavy Ion Therapy ◽  
Heavy Ion Radiation

Background: Heavy ion radiation has more advantages than traditional radiation therapy in the treatment of cancer, mainly because of its superior biological effects. However, there is currently no reliable evidence that heavy ion radiation can induce cell death in hydatid cysts at the cellular and molecular level. In addition, we believe heavy ion therapy could be a potential alternative approach for the treatment of hydatid cysts. Methodology/Principal Finding: The hydatid cysts and protoscolices were obtained from an experimentally infected KunMing mice. LD50 was used to evaluate the death of the protoscolex. The cellular and ultrastructure of the parasites were observed under light and electron microscopes, the damage and copy numbers of mitochondrial DNA (mtDNA) were decided by QPCR. The apoptosis was evaluated by the expression and activity of caspase3. Dose-dependent ionizing radiation induced damage to the initial mtDNA. Echinococcosis cyst after ionizing radiation showed sparse cytoplasm, disorganized and clumped organelles, huge vacuoles, and villus deletions. The kinetic of DNA repair activity after X-ray irradiation was faster than those after carbon-ion irradiation. High doses of carbon ion radiation caused irreversible attenuation of mitochondrial DNA. Cysts showed obvious reduction in size after radiation. Carbon ion radiation was more effective than X-ray radiation in inhibiting hydatid cysts. Conclusions: These studies provide evidence that heavy-ion radiation can cause the extinction of hydatid cysts in vitro. The carbon-ion radiation is more advantageous than X-ray radiation in suppress hydatid cyst.

Heavy ion radiation damage annealing models-a new interpretation

1989 ◽  
Vol 107 (2-4) ◽  
pp. 167-173 ◽  
Author(s):  
R. K. Bhatia ◽  
H. S. Virk
Keyword(s):  
Radiation Damage ◽  
Heavy Ion ◽  
Heavy Ion Radiation ◽  
New Interpretation

A 100 keV heavy ion accelerator for the study of irradiation damage

1962 ◽  
Vol 4 (2) ◽  
pp. 89-93 ◽  
Author(s):  
R W Barnfield ◽  
B W Farmery ◽  
L C W Hobbis ◽  
R S Nelson ◽  
M W Thompson
Keyword(s):  
Heavy Ion ◽  
Irradiation Damage ◽  
Heavy Ion Accelerator ◽  
Ion Accelerator

Plasma lens for the heavy ion accelerator at ITEP

2008 ◽  
Vol 5 (7) ◽  
pp. 582-585 ◽  
Author(s):  
M. M. Basko ◽  
A. A. Drozdovskii ◽  
A. A. Golubev ◽  
K. L. Gubskii ◽  
D. D. Iosseliani ◽  
...  
Keyword(s):  
Heavy Ion ◽  
Heavy Ion Accelerator ◽  
Ion Accelerator

Status of the VICKSI Heavy Ion Accelerator

1975 ◽  
Vol 22 (3) ◽  
pp. 1643-1646 ◽  
Author(s):  
W. Busse ◽  
B. Efken ◽  
D. Hilscher ◽  
H. Homeyer ◽  
H. Lettau ◽  
...  
Keyword(s):  
Heavy Ion ◽  
Heavy Ion Accelerator ◽  
Ion Accelerator

HIRFL Environment Temperature and Humidity Monitoring System Research and Design

2014 ◽  
Vol 651-653 ◽  
pp. 436-439
Author(s):  
Yun Jie Li ◽  
Yan Yu Wang ◽  
Jia Yin ◽  
Cheng Quan Pei ◽  
Xiao Li Ma
Keyword(s):  
Monitoring System ◽  
Basic Structure ◽  
Heavy Ion ◽  
Safe Operation ◽  
Ion Accelerator ◽  
Environment Temperature ◽  
Temperature And Humidity ◽  
Control And Monitoring System ◽  
Research And Design ◽  
Humidity Monitoring

HIRFL is an all-powerful heavy ion accelerator which is built by IMP of Chinese Academy of Science. It bears a lot of research tasks for china. Its control and monitoring system is very complex and very important, directly determines whether HIRFL safe operation. This paper is to study its much control and monitoring system of temperature and humidity monitoring system. Began to study the basic structure of the hardware, a specific architecture and specific HMI is designed, as well as the situation in the field installation deployment.

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