Sequential Evaluation of Immunoreactivity in Patients with Melanoma Undergoing Surgery and Adjuvant Therapy

1980 ◽  
Vol 66 (1) ◽  
pp. 59-76 ◽  
Author(s):  
Renzo Dionigi ◽  
Lorenzo Dominioni ◽  
Francesco Gnes ◽  
Alberto Bonera ◽  
Ubaldo Prati ◽  
...  
Keyword(s):  
Adjuvant Therapy ◽  
T Lymphocyte ◽  
Cellular Immunity ◽  
Postoperative Period ◽  
Phagocytic Activity ◽  
Early Postoperative Period ◽  
Factor B ◽  
Total Lymphocyte ◽  
Blastogenic Response

The immunologic profile of 15 patients undergoing surgery and adjuvant chemoimmunotherapy for cutaneous melanoma was studied for a mean period of 18 months. In vivo cellular immunity was assayed by evaluation of delayed hypersensitivity response (DHR) to primary antigen and a panel of recall antigens. In vitro cellular immunity was evaluated by means of total and T-lymphocyte counts in peripheral blood and by the lymphocyte blastogenic response to phytohemagglutinin stimulation. Humoral immunity was assayed by determining the serum levels of IgG, IgA and IgM and of complement components C3c, C4 and Factor B. Phagocytic activity was studied by testing leukocyte chemotaxis, neutrophil phagocytosis and leukocyte random migration. The in vitro parameters were determined preoperatively at diagnosis, 6 times during the first 2 postoperative weeks, and then every month during adjuvant therapy. No correlation was found between DHR and clinico-pathologic stage of tumor, or with subsequent clinical course. Significant depression of total lymphocyte and T-lymphocyte count and blastogenic response of lymphocytes was found at diagnosis. The lymphocyte response to PHA decreased significantly in the early postoperative period but returned to preoperative levels one week after surgery. Periodic fluctuations of lymphocyte blastogenic response and progressive decrease of total lymphocyte counts and T-lymphocyte counts were observed during the 18-month follow-up. No significant alterations of immunoglobulin levels were recorded at diagnosis or during the postoperative period. Complement levels were within normal values preoperatively; in the early postoperative period a transient increase of C3c, C4 and Factor B was recorded, then complement levels progressively decreased. Parameters of phagocytic activity were normal at diagnosis and fluctuated within the normal range throughout the whole period of study.

Patients with abnormal proportions of T-lymphocyte subsets have reduced in vitro cellular immunity

1983 ◽  
Vol 26 (1) ◽  
pp. 126-136 ◽  
Author(s):  
David S. Chudwin ◽  
Morton J. Cowan ◽  
Diane W. Wara ◽  
Arthur J. Ammann
Keyword(s):  
T Lymphocyte ◽  
Cellular Immunity ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets

scholarly journals ECTOPIC PREGNANCY AND ACUTE APPENDICITIS DURING INTRAUTERIN PREGNANCY AFTER IN VITRO FERTILIZATION

2018 ◽  
Vol 2 (5) ◽  
pp. 169-172
Author(s):  
Наталья Протопопова ◽  
Natalya Protopopova ◽  
Елена Дружинина ◽  
Elena Druzhinina ◽  
Альбина Лабыгина ◽  
...  
Keyword(s):  
Acute Appendicitis ◽  
Ectopic Pregnancy ◽  
Postoperative Period ◽  
Clinical Symptoms ◽  
Uterine Cavity ◽  
Early Postoperative Period ◽  
Chlamydia Infection ◽  
Surgical Department ◽  
Ultrasound Study

The paper presents a clinical case of the Patient T., 34 years old, who underwent the treatment in the Department of ART of the Regional Perinatal Center for tubal peritoneal infertility for seven years. In the anamnesis: one spontaneous uterine pregnancy, culminating in medical abortion for up to 12 weeks, treatment for chronic salpingo-oophoritis, tu- bectomy on the right side for a progressing ectopic pregnancy. The patient denied the presence of Chlamydia infection in the anamnesis. Studies on sexually transmitted infections before the IVF program were negative. During laparo- scopic tubectomy, no endometriotic foci were found. There were four programs of ART in anamnesis: in 2009, 2012, 2014, 2016. During the fourth ART program in 2016, two embryos were transferred to the uterine cavity, resulting in a uterine pregnancy in combination with an ectopic pregnancy diagnosed in the ultrasound study at 4 to 5 weeks of gestation, in the absence of clinical symptoms. The patient underwent emergency laparoscopic tubectomy on the left, the course of the postoperative period proceeded without complications. Later, in the period of uterine pregnancy of 7–8 weeks, with a clinical picture of acute appendicitis, the woman was hospitalized in the surgical department where she underwent laparoscopic appendectomy. Later the pregnancy proceeded without complications, at 39 weeks the planned cesarean section was performed, a girl weighing 3480 grams, 51 cm long with an Apgar score of 9–10 points was extracted on the operation. The course of the early postoperative period without complications, the patient was discharged with the child on the 4th day.

ОПЫТ ИССЛЕДОВАНИЯ РАЗЛИЧНЫХ СВОЙСТВ МЕСТНЫХ КРОВООСТАНАВЛИВАЮЩИХ СРЕДСТВ

Innova ◽  
2021 ◽  
Vol 22 (1) ◽  
pp. 20-24
Author(s):  
Вячеслав Александрович Липатов ◽  
Дмитрий Андреевич Северинов ◽  
Елена Леонидовна Пучкова
Keyword(s):  
Postoperative Period ◽  
Personal Experience ◽  
Early Postoperative Period ◽  
Hemostatic Agent ◽  
Present Stage ◽  
Hemostatic Agents ◽  
In Vivo Experiments

Bleeding during surgery or in the early postoperative period remains one of the main problems of surgery, in particular surgery of parenchymal organs. At the present stage, various local hemostatic agents are widely used. To select a specific hemostatic agent in certain operations, it is necessary to have an understanding of the properties of various application hemostatic agents, their effectiveness, features of biodegradation, etc. For this purpose, methods for studying the properties of hemostatic agents in in vitro and in vivo experiments are being developed. Purpose of research. Description of the stages and main methods of research of various properties of spongy hemostatic agents based on the authors ' personal experience.

scholarly journals Treatment of retroauricular keloids: Revision of cases treated at the ENT service of HC/UFPR

2012 ◽  
Vol 16 (02) ◽  
pp. 195-200 ◽  
Author(s):  
Annelyse Ballin ◽  
Renata Becker ◽  
Talita Ribeiro ◽  
Juliana Cavichiolo ◽  
Carlos Ballin ◽  
...  
Keyword(s):  
Adjuvant Therapy ◽  
Treatment Option ◽  
Postoperative Period ◽  
Early Postoperative Period ◽  
Benign Tumors ◽  
Prospective Analysis ◽  
Service Center ◽  
New Techniques ◽  
Corticosteroid Administration ◽  
Nose And Throat

Summary Introduction: Keloids are benign tumors arising from abnormal healing of the skin, and there are several procedures available for their treatment. Objective: The objective of this study was to evaluate the outcomes of patients undergoing treatment of keloids after ear, nose, and throat (ENT) surgeries at our service center. Method: We conducted thorough, retrospective and prospective analysis of records of patients undergoing treatment of retroauricular keloids at our center. Results: Nine patients were evaluated, and 6 underwent resection and adjuvant beta-therapy, 2 underwent resection with local application of corticosteroids, and only 1 underwent resection without adjuvant therapy. There was no recurrence of keloids in patients that were treated with beta-therapy in the early postoperative period. One patient had relapsed despite corticosteroid administration and late beta-therapy. Discussion: Several techniques have been used for the treatment of retroauricular keloids, and beta-therapy is thought to yield the best results, followed by the use of intralesional corticosteroids. Conclusion: Treatment of retroauricular keloids remains a challenge. While new techniques are being developed, resection followed by early beta-therapy is still the best treatment option.

scholarly journals Optimization technique for stenting of ostial vertebral artery stenosis

2020 ◽  
Vol 34 (4) ◽  
pp. 34-44
Author(s):  
Yu.V. Cherednychenko ◽  
A.Yu. Miroshnychenko ◽  
M.O. Zorin ◽  
L.A. Dzyak ◽  
O.S. Tsurkalenko
Keyword(s):  
Vertebral Artery ◽  
Postoperative Period ◽  
Optimization Technique ◽  
Carotid Stenting ◽  
Early Postoperative Period ◽  
Long Term Results ◽  
In Vitro Tests ◽  
Complex Method ◽  
Vertebral Arteries

Objective ‒ to develop a technique for endovascular treatment of symptomatic ostial stenosis of the vertebral arteries, which allows to minimize risks of delayed stent breakage and restenosis. Materials and methods. This is analysis of prospectively collected data from patients presenting from 2016 to 2019 in the endovascular center of the Dnepropetrovsk Regional Clinical Hospital named after I.I. Mechnikov. One hundred four stents were placed in 99 patients using the author’s complex method, which is based on our modification of Szabo technique. The principles of the method were developed based on a literature review and in vitro tests using 7 silicone models of the initial segments of the subclavian and vertebral arteries with different angles of divergence of the vertebral arteries (30, 45, 60, 90, 120, 135, 150°) and 9 balloon mounted drug-eluting stents with open-cell design Resolute (Medtronic).Results. There were no cases of displacement of the stent proximally or distally during implantation. In all cases, stents were implanted in the affected segment exactly and did not prolapse more than 1 mm beyond the ostium of the vertebral artery into the subclavian artery. There were no «clinical» ischemic complications in the early postoperative period. In 5 cases, isolated subclinical ischemic lessions in the carotid circulation were revealed during one-session stenting of ostial stenosis of the vertebral arteries and carotid stenting. In the posterior circulation, ischemic lessions on MRI in the early postoperative period were not detected in any observations.Conclusions. Developed complex stenting method based on our modification of Szabo technique allows us to achieve optimal long-term results of stenting of symptomatic ostial stenosis of the vertebral arteries.

Cellular immune defect caused by postsurgical radiation therapy in patients with head and neck cancer

1998 ◽  
Vol 119 (4) ◽  
pp. 412-417 ◽  
Author(s):  
Matthias Tisch ◽  
Florίan Heimlich ◽  
Volker Daniel ◽  
Gerhard Opelz ◽  
Heinz Maier
Keyword(s):  
Radiation Therapy ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
T Lymphocyte ◽  
Neck Cancer ◽  
Cellular Immunity ◽  
Lymphocyte Count ◽  
Radiation Treatment ◽  
Lymphocyte Stimulation

The effects of locoregional postoperative radiation therapy (60 Gy on average) on cellular immunity were investigated in 11 patients with squamocellular carcinomas of the oral cavity, pharynx, or larynx. During radiation treatment, the total lymphocyte counts, CD8+ T-lymphocyte count, and especially CD4+ T-lymphocyte count decreased significantly. The mean CD4+ T-lymphocyte counts dropped from an average of 739/μl to 183/μl ( p <0.001), and the CD4+/CD8+ quotient also decreased significantly. Not only the lymphocyte counts but also the in vitro lymphocyte stimulation responses to several mitogens decreased, with reductions averaging 10% to 50% of normal responses by the end of radiation therapy. Within 3 to 4 weeks after radiation therapy, the CD4+ T-lymphocyte counts and the in vitro lymphocyte stimulation responses showed a tendency toward normalization. This study shows that postoperative locoregional radiation therapy in patients with head and neck cancer induces a severe generalized impairment of cellular immunity.

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