scholarly journals Studies of Autofluorescence in Experimentally Induced Cerebral Necrosis in Pigs

1980 ◽  
Vol 17 (2) ◽  
pp. 226-233 ◽  
Author(s):  
J. Y. S. Lee ◽  
P. B. Little

Lesions of cerebrocortical necrosis experimentally induced in pigs by transcalvarial freezing with liquid nitrogen were slightly fluorescent 48 hours after surgery. Fluorescence increased greatly thereafter and was most marked grossly under ultraviolet illumination with a wavelength of 366 nm at 7 days. Fluorescence of the necrotic focus persisted up to 35 days after surgery. Detection of cerebrocortical necrosis by ultraviolet illumination of fresh specimens during gross inspection is useful in determining the extent and distribution of lesions. This would aid more accurate selection of brain sections for histological examination in various encephalopathies.

2017 ◽  
Author(s):  
Siti Aminah Zakaria ◽  
Zuhailawati Hussain ◽  
Anasyida Abu Seman

1982 ◽  
Vol 35 (5) ◽  
pp. 545 ◽  
Author(s):  
DE Hollis ◽  
RE Chapman ◽  
JA Hemsley

Four medium- and six strong-wool Merino sheep were exposed to continuous simulated rain (4'5 mm/h) for periods ranging from 3 to 9 days. In response, the sheep developed characteristics typical of fleece-rot lesions such as exudative encrustation' and bacterial discoloration of the fleece. Skin samples were taken periodically for histological examination from the trunk region of the sheep prior to, during, and after wetting.


2007 ◽  
Vol 2 (3) ◽  
pp. 313-357 ◽  
Author(s):  
Koenraad Kuiper ◽  
Marie-Elaine van Egmond ◽  
Gerard Kempen ◽  
Simone Sprenger

Only relatively recently have theories of speech production concerned themselves with the part idioms and other multi-word lexical items (MLIs) play in the processes of speech production. Two theories of speech production which attempt to account for the accessing of idioms in speech production are those of Cutting and Bock (1997) and superlemma theory (Sprenger, 2003; Sprenger, Levelt, & Kempen, 2006). Much of the data supporting theories of speech production comes either from time course experiments or from slips of the tongue (Bock & Levelt, 1994). The latter are of two kinds: experimentally induced (Baars, 1992) or naturally observed (Fromkin, 1980). Cutting and Bock use experimentally induced speech errors while Sprenger et al. use time course experiments. The missing data type that has a bearing on speech production involving MLIs is that of naturally occurring slips. In this study the impact of data taken from naturally observed slips involving English and Dutch MLIs are brought to bear on these theories. The data are taken initially from a corpus of just over 1000 naturally observed English slips involving MLIs (the Tuggy corpus). Our argument proceeds as follows. First we show that slips occur independent of whether or not there are MLIs involved. In other words, speech production proceeds in certain of its aspects as though there were no MLI present. We illustrate these slips from the Tuggy data. Second we investigate the predictions of superlemma theory. Superlemma theory (Sprenger et al., 2006) accounts for the selection of MLIs and how their properties enter processes of speech production. It predicts certain activation patterns dependent on a MLI being selected. Each such pattern might give rise to slips of the tongue. This set of predictions is tested against the Tuggy data. Each of the predicted activation patterns yields a significant number of slips. These findings are therefore compatible with a view of MLIs as single units in so far as their activation by lexical concepts goes. However, the theory also predicts that some slips are likely not to occur. We confirm that such slips are not present in the data. These findings are further corroborated by reference a second smaller dataset of slips involving Dutch MLIs (the Kempen corpus). We then use slips involving irreversible binomials to distinguish between the predictions of superlemma theory which are supported by slips involving irreversible binomials and the Cutting and Bock model’s predictions for slips involving these MLIs which are not.


Author(s):  
A. Gulov ◽  
A. Laskin

Purpose: Conducting a honey diluent test for creeples of sperm of a drone honey bee.Materials and methods. The material for the research served a sperm of the milled drone drums of the "Prioksky" type of the Midway breed of bees. The selection of sperm was carried out in June-July 2020 g by the method of artificial stimulation of the turning of the endofalosha in half-armed drones aged 25-30 days. The rock type "Prioksky" of the middle Russian breed of bees. Before freezing, the sperm was stored in glass capillaries in the cooled state at 3 ° C for 2 months. The following composition of the diluent was tested - 10% honey, lactose, sucrose, egg yolk and dimethyl sulfoxide.Results. Studies have shown the viability of sperm at 64.0 ± 1.8% (41.5-83.7), and a total mobility of 2.2 ± 0.6% (0-11.5). To evaluate the fertilizing ability of sperm, carried out artificial insemination of 10 bee modules. In 4 seeded bees dykens, the presence of sperm in a seed-hearter with a concentration of sperm from 0.22-4.4 million / μl is revealed. In paired eggs of three other seeded matters, the presence of sperm and the complete absence of spermatozoa in the seed-receptionist are recorded.Conclusion. Tests of the honey diluent for deep freezing sperm of the drone honey bees in liquid nitrogen confirmed its cryophylactic properties.


2008 ◽  
Vol 13 (3) ◽  
pp. 259-264 ◽  
Author(s):  
Yoshikazu Tanzawa ◽  
Hiroyuki Tsuchiya ◽  
Norio Yamamoto ◽  
Kenshi Sakayama ◽  
Hiroshi Minato ◽  
...  

2020 ◽  
Author(s):  
Andrew J. Boria ◽  
Carlos Perez-Torres

Abstract Background: Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy results. Our goal was to determine how the train and sex of the mice selection would affect the development of radiation necrosis in our murine model of radiation-induced cerebral necrosis. Methods: We generated this model by using a preclinical irradiator to irradiate a sub-hemispheric portion of the brain of mice with single-fraction doses of 80 Gy. Eight possible combinations of mice made up of two different with two substrains each(BALB/cN, BALB/cJ, C57BL/6N, and C57BL/6J) and both sexes were irradiated in this study. Radiation necrosis development was tracked up to eight weeks with a 7T Bruker MRI utilizing T2-weighted and post-contrast T1-weighted imaging. MRI results were compared to and validated with the use of histology which utilized a scale from 0-3 in ascending order of damage. Results: Both time post-irradiation and strain (BALB/c vs C57BL/6) were significant factors affecting radiation necrosis development. Sex was in general not a statistically significant parameter in terms of radiation necrosis development. Conclusion: Mouse strain thus need to be considered when evaluating the results of necrosis models. However, sex does not appear to be a variable needing major consideration.


Author(s):  
Ana Cojocaru ◽  
◽  
Veronica Svet ◽  

Organ-сonserving operations in breast cancer include performing extended sectoral resections with regional lymphadenectomy. In the case of these interventions, immediate histological examination of the safe margins and subareolar area will be mandatory. Overly economical tactics are associated with the risk of breast cancer recurrence. Organ-preserving breast cancer surgeries, supplemented with radiation therapy, are a safe alternative to mastectomy with the correct selection of patients with early forms.


2020 ◽  
Author(s):  
Andrew J. Boria ◽  
Carlos Perez-Torres

Abstract Background: Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy results. Our goal was to determine how the train and sex of the mice selection would affect the development of radiation necrosis in our murine model of radiation-induced cerebral necrosis.Methods: We generated this model by using a preclinical irradiator to irradiate a sub-hemispheric portion of the brain of mice with single-fraction doses of 80 Gy. Eight possible combinations of mice made up of two different with two substrains each(BALB/cN, BALB/cJ, C57BL/6N, and C57BL/6J) and both sexes were irradiated in this study. Radiation necrosis development was tracked up to eight weeks with a 7T Bruker MRI utilizing T2-weighted and post-contrast T1-weighted imaging. MRI results were compared to and validated with the use of histology which utilized a scale from 0-3 in ascending order of damage.Results: Both time post-irradiation and strain (BALB/c vs C57BL/6) were significant factors affecting radiation necrosis development. Sex was in general not a statistically significant parameter in terms of radiation necrosis development.Conclusion: Mouse strain thus need to be considered when evaluating the results of necrosis models. However, sex does not appear to be a variable needing major consideration.


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