scholarly journals Histological examination of frozen autograft treated by liquid nitrogen removed 6 years after implantation

2008 ◽  
Vol 13 (3) ◽  
pp. 259-264 ◽  
Author(s):  
Yoshikazu Tanzawa ◽  
Hiroyuki Tsuchiya ◽  
Norio Yamamoto ◽  
Kenshi Sakayama ◽  
Hiroshi Minato ◽  
...  
1980 ◽  
Vol 17 (2) ◽  
pp. 226-233 ◽  
Author(s):  
J. Y. S. Lee ◽  
P. B. Little

Lesions of cerebrocortical necrosis experimentally induced in pigs by transcalvarial freezing with liquid nitrogen were slightly fluorescent 48 hours after surgery. Fluorescence increased greatly thereafter and was most marked grossly under ultraviolet illumination with a wavelength of 366 nm at 7 days. Fluorescence of the necrotic focus persisted up to 35 days after surgery. Detection of cerebrocortical necrosis by ultraviolet illumination of fresh specimens during gross inspection is useful in determining the extent and distribution of lesions. This would aid more accurate selection of brain sections for histological examination in various encephalopathies.


Author(s):  
Roslizawaty Roslizawaty ◽  
Syafruddin Syafruddin ◽  
Tongku Nizwan Siregar ◽  
Azrin . ◽  
Zuhrawati Zuhrawati ◽  
...  

This research aimed to determine Aceh cattle ovarian follicle morphological integrity after vitrified by various cryoprotectants. Cryoprotectants used in this research were 30% ethylene glycol (EG), 30% dimethyl suphocide (DMSO), and combination of 15% EG + 15% DMSO. Prior to vitrification process, ovaries were cleansed by phosphate buffered saline (PBS) and then cut into ±1 mm³. Ovaries were consecutively submerged into the following liquid for 5 minutes each: PBS+ 0.25 M sucrose; PBS+ 0.5 M sucrose; PBS+ 0.5 M sucrose + 10% cryoprotectants; and PBS+ 0.5 M sucrose + 30% cryoprotectants. Then, ovaries were packed into straws with ±7 cm in length and ± 6 mm in diameter. Before kept in liquid nitrogen, ovaries were first exposed to nitrogen fume for 10 second. After being stored for 1 day, the ovaries were proceed for histological examination. The result showed that Aceh cattle ovarian follicle after vitrification using 30% EG yields the best morphological integrity. Cumulus oophorus, zona pellucida, granulose cell arrangement, theca interna, and theca externa cells were observed clearer in ovary which was vitrified with 30 % EG than those with 30% DMSO and combination of 15% EG + 15% DMSO. As conclusion, 30% EG was able to protect ovary morphological integrity better than 15 % EG + 15% DMSO and 30% DMSO. Furthermore, combination of 15% EG+ 15 % DMSO was relatively better in protecting ovary follicle morphological integrity compared to 30% DMSO.


2009 ◽  
Vol 14 (6) ◽  
pp. 761-768 ◽  
Author(s):  
Yoshikazu Tanzawa ◽  
Hiroyuki Tsuchiya ◽  
Toshiharu Shirai ◽  
Katsuhiro Hayashi ◽  
Zen Yo ◽  
...  

Author(s):  
Claude Lechene

Electron probe microanalysis of frozen hydrated kidneysThe goal of the method is to measure on the same preparation the chemical elemental content of the renal luminal tubular fluid and of the surrounding renal tubular cells. The following method has been developed. Rat kidneys are quenched in solid nitrogen. They are trimmed under liquid nitrogen and mounted in a copper holder using a conductive medium. Under liquid nitrogen, a flat surface is exposed by sawing with a diamond saw blade at constant speed and constant pressure using a custom-built cryosaw. Transfer into the electron probe column (Cameca, MBX) is made using a simple transfer device maintaining the sample under liquid nitrogen in an interlock chamber mounted on the electron probe column. After the liquid nitrogen is evaporated by creating a vacuum, the sample is pushed into the special stage of the instrument. The sample is maintained at close to liquid nitrogen temperature by circulation of liquid nitrogen in the special stage.


Author(s):  
Louis T. Germinario

A liquid nitrogen stage has been developed for the JEOL JEM-100B electron microscope equipped with a scanning attachment. The design is a modification of the standard JEM-100B SEM specimen holder with specimen cooling to any temperatures In the range ~ 55°K to room temperature. Since the specimen plane is maintained at the ‘high resolution’ focal position of the objective lens and ‘bumping’ and thermal drift la minimized by supercooling the liquid nitrogen, the high resolution capability of the microscope is maintained (Fig.4).


Author(s):  
O. T. Inal ◽  
L. E. Murr

When sharp metal filaments of W, Fe, Nb or Ta are observed in the field-ion microscope (FIM), their appearance is differentiated primarily by variations in regional brightness. This regional brightness, particularly prominent at liquid nitrogen temperature has been attributed in the main to chemical specificity which manifests itself in a paricular array of surface-atom electron-orbital configurations.Recently, anomalous image brightness and streaks in both fcc and bee materials observed in the FIM have been shown to be the result of surface asperities and related topographic features which arise by the unsystematic etching of the emission-tip end forms.


Author(s):  
T. G. Naymik

Three techniques were incorporated for drying clay-rich specimens: air-drying, freeze-drying and critical point drying. In air-drying, the specimens were set out for several days to dry or were placed in an oven (80°F) for several hours. The freeze-dried specimens were frozen by immersion in liquid nitrogen or in isopentane at near liquid nitrogen temperature and then were immediately placed in the freeze-dry vacuum chamber. The critical point specimens were molded in agar immediately after sampling. When the agar had set up the dehydration series, water-alcohol-amyl acetate-CO2 was carried out. The objectives were to compare the fabric plasmas (clays and precipitates), fabricskeletons (quartz grains) and the relationship between them for each drying technique. The three drying methods are not only applicable to the study of treated soils, but can be incorporated into all SEM clay soil studies.


Author(s):  
Shaul Barkan

Cooling down solid state detecors, with other different way then liquid Nitrogen, is a goal of many vendors and customers since the invention of these detectors. THe disadvantage of the common way of liquid Nitrogen is first the inavailibility of the LN in many uses (like space military and any other applications that are not done inside a well organize Laboratory). The use of LN also considers as a Labor consumer in addition to the big dewar that has to be added to any detector for storing the LN, the boiling of the LN, may cause microphonics problesm and the refiling of the dewar in many Labs is a complicated process due to inconvenience location of the microscope.In this paper I will show a spectra result of 10mm2 SiLi detector for microanalysis use, cooled by peltier cooler. The peltier cooler has the advantage of non-microphonics and non-labor needed (like adding LN to the dewar).


Author(s):  
M.K. Lamvik ◽  
D.A. Kopf ◽  
S.D. Davilla ◽  
J.D. Robertson

Last year we reported1 that there is a striking reduction in the rate of mass loss when a specimen is observed at liquid helium temperature. It is important to determine whether liquid helium temperature is significantly better than liquid nitrogen temperature. This requires a good understanding of mass loss effects in cold stages around 100K.


Author(s):  
John G. Sheehan

The goal is to examine with high resolution cryo-SEM aqueous particulate suspensions used in coatings for printable paper. A metal-coating chamber for cryo-preparation of such suspensions was described previously. Here, a new conduction-cooling system for the stage and cold-trap in an SEM specimen chamber is described. Its advantages and disadvantages are compared to a convection-cooling system made by Hexland (model CT1000A) and its mechanical stability is demonstrated by examining a sample of styrene-butadiene latex.In recent high resolution cryo-SEM, some stages are cooled by conduction, others by convection. In the latter, heat is convected from the specimen stage by cold nitrogen gas from a liquid-nitrogen cooled evaporative heat exchanger. The advantage is the fast cooling: the Hexland CT1000A cools the stage from ambient temperature to 88 K in about 20 min. However it consumes huge amounts of liquid-nitrogen and nitrogen gas: about 1 ℓ/h of liquid-nitrogen and 400 gm/h of nitrogen gas. Its liquid-nitrogen vessel must be re-filled at least every 40 min.


Sign in / Sign up

Export Citation Format

Share Document