Pathology of Infectious Diarrhea of Infant Rats (IDIR) Induced by an Antigenically Distinct Rotavirus

1989 ◽  
Vol 26 (5) ◽  
pp. 376-385 ◽  
Author(s):  
A. C. Huber ◽  
R. H. Yolken ◽  
L. C. Mader ◽  
J. D. Strandberg ◽  
S. L. Vonderfecht
Keyword(s):  
Small Intestine ◽  
Post Inoculation ◽  
Diagnostic Features ◽  
Distal Small Intestine ◽  
Precursor Material ◽  
Viral Particles ◽  
Intestinal Segments ◽  
Villous Height ◽  
Small Intestinal ◽  
Group B

Suckling rats were inoculated with a group B rotavirus to determine the progression of the morphologic changes induced in the intestine by this virus. Several changes were observed by light microscopy 1 day after viral inoculation: shortening of small intestinal villi, villous epithelial necrosis, and villous epithelial syncytia. The lesions were most often present in the distal small intestine, although other small intestinal segments were affected to a lesser degree. By day 3 post-inoculation, epithelial necrosis, and syncytia were no longer present; however, the villous epithelium was disorganized and irregularly vacuolated, and intestinal crypt epithelium was hyperplastic. Alterations in villous height to crypt depth ratios were present in portions of the small intestine for the remainder of the 12-day study period. Epithelial syncytia appeared to form by the breakdown of the lateral interdigitating membranes of the absorptive villous epithelium. Viral particles, abundant in the syncytia, appeared to form from amorphous or reticular arrays of viral precursor material. Group B rotaviral antigens, as detected by indirect immunofluorescence, were present in large amounts in the small intestinal villous epithelium only on the first day after viral inoculation. These studies show that two important diagnostic features of group B rotaviral infections of rats, epithelial syncytia and viral antigen as determined by immunofluorescence, are present only on the first day of disease. These findings should be taken into consideration when attempting to diagnose disease induced by this agent.

scholarly journals Ultrastructural Changes in the Small Intestine of Neonatal Calves with Enteric Colibacillosis

1982 ◽  
Vol 19 (2) ◽  
pp. 190-201 ◽  
Author(s):  
G. R. Pearson ◽  
E. F. Logan
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Small Groups ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Cytoplasmic Inclusions ◽  
Distal Small Intestine ◽  
Enteropathogenic Strain ◽  
Neonatal Calves ◽  
Small Intestines

The small intestines of calves inoculated orally with the enteropathogenic strain of Escherichia coli 0101:K'B41′, K99 were examined by electron microscopy at 3, 6, 12, 16, 21, 36, 69, 70 and 72 hours after inoculation. The challenge organism adhered to the mucosa of the distal small intestine from six hours post-inoculation. Bacteria were separated from the microvillous brush border by a gap of 200 to 300 nm in which bacterial fimbriae and the microvillous glycocalyx were seen. Bacteria never were found in epithelial cells but were present in macrophages in the lamina propria from 12 hours. At three and six hours, cytopathic changes were not seen in the small intestine, but from 12 hours epithelial cells on affected villi had blunt and thick microvilli and contained cytoplasmic inclusions. Epithelial cells were seen frequently in the process of extrusion from the villi, either singly, in small groups, or as ribbons of cells. Intervillous bridges, characteristic of villous fusion, were seen frequently from 69 hours.

Role of 5-HT in cholera toxin-induced mucin secretion in the rat small intestine

1996 ◽  
Vol 270 (6) ◽  
pp. G1001-G1009 ◽  
Author(s):  
B. A. Moore ◽  
K. A. Sharkey ◽  
M. Mantle
Keyword(s):  
Small Intestine ◽  
Receptor Antagonist ◽  
Cholera Toxin ◽  
Receptor Subtypes ◽  
Rat Small Intestine ◽  
Mucin Secretion ◽  
Common Pathway ◽  
Distal Small Intestine ◽  
Intestinal Segments

We examined the role of 5-hydroxytryptamine (5-HT) in cholera toxin (CT)-induced mucin secretion in the proximal and distal regions of the rat small intestine. Neither the 5-HT2 receptor antagonist ketanserin nor the cyclooxygenase inhibitor indomethacin was capable of inhibiting choleraic mucin secretion. However, in the presence of the mixed 5-HT3/4 receptor antagonist tropisetron at doses that block both receptor subtypes, the secretory response was reduced to baseline levels in the proximal and distal small intestine. The selective 5-HT3 receptor antagonist ondansetron had no significant effect. These findings suggest that choleraic mucin secretion is mediated primarily through the activation of a 5-HT4-like receptor. Mucin secretion in response to the exogenous application of 5-HT occurs via two pathways: one is mediated by a 5-HT4-like receptor and is capsaicin sensitive but tetrodotoxin (TTX) insensitive, and one lacks the capsaicin-sensitive 5-HT4-mediated response but is TTX sensitive. Both converge on a common pathway that is cholinergic. No significant differences were observed between proximal and distal intestinal segments.

Evidence that neuronally released vasoactive intestinal polypeptide inhibits the release of serotonin from enterochromaffin cells of the guinea pig small intestine

1991 ◽  
Vol 124 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Kurt Racké ◽  
Harald Schwörer ◽  
Denis V. Agoston ◽  
Heinz Kilbinger
Keyword(s):  
Small Intestine ◽  
Guinea Pig ◽  
Vasoactive Intestinal Polypeptide ◽  
Inhibitory Effect ◽  
Good Evidence ◽  
Muscarine Receptors ◽  
Intestinal Segments ◽  
Hydroxyindoleacetic Acid ◽  
Small Intestinal ◽  
Intestinal Polypeptide

Abstract. Isolated small intestinal segments of the guinea pig were arterially perfused and the release of serotonin (5-hydroxytryptamine) and 5-hydroxyindoleacetic acid into the portal venous effluent was determined by HPLC with electrochemical detection. Test substances were intra-arterially applied. The muscarine receptor agonist oxotremorine (1 μmol/l inhibited the release of 5-hydroxytryptamine by about 50%. In the presence of the neurotoxin tetrodotoxin, oxotremorine enhanced the release of 5-hydroxytryptamine by 145%, indicating that the inhibitory effect of oxotremorine was mediated by the release of a neurotransmitter. Exogenous vasoactive intestinal polypeptide ( 1-100 pmol/l inhibited the release of 5-hydroxytryptamine by about 50%, an effect antagonized by a specific antibody to vasoactive intestinal polypeptide. This antibody to vasoactive intestinal polypeptide, on its own, had no effect on the release of 5-hydroxytryptamine. However, it prevented the inhibitory effect of oxotremorine. In the presence of the antibody to vasoactive intestinal polypeptide, unlike in the presence of tetrodotoxin, oxotremorine did not stimulate the release of 5-hydroxytryptamine. In conclusion, activation of neuronal muscarine receptors in the guinea pig small intestine enhances the release of several neurotransmitters which can inhibit the release of 5-hydroxytryptamine. The present experiments provide good evidence that vasoactive intestinal polypeptide is one of them.

Variation in Rotavirus Virulence: A Comparison of Pathogenesis in Calves between Two Rotaviruses of Different Virulence

1993 ◽  
Vol 30 (3) ◽  
pp. 223-233 ◽  
Author(s):  
G. A. Hall ◽  
J. C. Bridger ◽  
K. R. Parsons ◽  
R. Cook
Keyword(s):  
Small Intestine ◽  
Virulent Strain ◽  
Villus Height ◽  
Distal Small Intestine ◽  
Cytopathic Effects ◽  
Proximal Small Intestine ◽  
Viral Particles ◽  
Virulent Virus ◽  
The Mean ◽  
Spread Of Infection

Variation in virulence between two bovine rotaviruses was investigated using ten female and ten male 10-day-old gnotobiotic calves of five breeds or cross breeds that were inoculated with a virulent strain or a strain of low virulence. Similar numbers of infectious viral particles were detected in feces of calves inoculated with either virus, but diarrhea, xylose malabsorption, and reduction of villus height occurred only after inoculation with virulent virus. The mean percentage of the area of the villus epithelium per villus immunostained for rotavirus antigen was eight times greater in calves inoculated with virulent virus, and the mean percentage of villi on which immunostained enterocytes were detected was twice as large in calves inoculated with virulent virus than in calves inoculated with the virus of low virulence. Mean crypt death and mean crypt cell production rates were increased after inoculation with either virus. Virulence was associated with extensive spread of infection through the small intestine, preferential colonization of the proximal small intestine, and marked damage to enterocytes and villi. The virus of low virulence infected the proximal small intestine poorly, and although it infected more enterocytes in the mid and distal small intestine and replicated in them, causing cytopathic effects, it did not damage intestinal structure and affect function.

Glucagon-like peptide-2 acutely increases proximal small intestinal blood flow in TPN-fed neonatal piglets

2006 ◽  
Vol 290 (2) ◽  
pp. R283-R289 ◽  
Author(s):  
John Stephens ◽  
Barbara Stoll ◽  
Jeremy Cottrell ◽  
Xiaoyan Chang ◽  
Michael Helmrath ◽  
...  
Keyword(s):  
Blood Flow ◽  
Small Intestine ◽  
Venous Blood ◽  
Mucosal Blood Flow ◽  
Tissue Blood Flow ◽  
Venous Blood Flow ◽  
Distal Small Intestine ◽  
Gut Hormone ◽  
Glucagon Like Peptide ◽  
Small Intestinal

Glucagon-like peptide-2 (GLP-2) is a gut hormone that is secreted in response to enteral feeding and stimulates small intestinal mucosal growth. We have previously shown that GLP-2 infusion acutely increases portal venous blood flow in TPN-fed piglets. The aim of this study was to localize the vasoactive effect of GLP-2 within the gastrointestinal tissues and other visceral organs in TPN-fed piglets. Tissue blood flow rates were quantified using fluorescent microsphere deposition in anesthetized TPN-fed piglets given intravenous infusion of GLP-2 at either 500 pmol·kg−1·h−1 (low GLP-2, n = 7 pigs) or 2,000 pmol·kg−1·h−1 (high GLP-2, n = 8 pigs) for 2 h. Compared with baseline, the low and the high GLP-2 treatment significantly increased the blood flow rate in the duodenum (+77%) and jejunum (+40% and 80%), respectively, but blood flow to the distal small intestine and colon (−15%) was unchanged or slightly decreased. Baseline mucosal blood flow was five-fold higher than serosal blood flow; however, high GLP-2 treatment increased serosal (+140%) to a larger degree than mucosal blood flow (+73%). The high GLP-2 dose increased pancreatic flow (+34%) but decreased blood flow in the kidneys (−14%) and stomach (−12%), whereas the spleen and brain were unaffected. These findings suggest that the acute GLP-2-mediated stimulation of portal blood flow in TPN-fed piglets occurs principally via increased blood flow through the superior mesenteric artery to the proximal small intestine, a tissue region where the GLP-2R mRNA abundance and trophic GLP-2 effects are greatest.

Functional reentry and circus movement arrhythmias in the small intestine of normal and diabetic rats

2012 ◽  
Vol 302 (7) ◽  
pp. G684-G689 ◽  
Author(s):  
Wim J. E. P. Lammers ◽  
B. Stephen ◽  
S. M. Karam
Keyword(s):  
Small Intestine ◽  
Slow Wave ◽  
Diabetic Rats ◽  
Intestinal Wall ◽  
Distal Small Intestine ◽  
Circus Movement ◽  
Conduction Velocities ◽  
Intestinal Segments ◽  
Reentrant Arrhythmia ◽  
Reentrant Arrhythmias

In a few recent studies, the presence of arrhythmias based on reentry and circus movement of the slow wave have been shown to occur in normal and diseased stomachs. To date, however, reentry has not been demonstrated before in any other part of the gastrointestinal system. No animals had to be killed for this study. Use was made of materials obtained during the course of another study in which 11 rats were treated with streptozotocin and housed with age-matched controls. After 3 and 7 mo, segments of duodenum, jejunum, and ileum were isolated and positioned in a tissue bath. Slow wave propagation was recorded with 121 extracellular electrodes. After the experiment, the propagation of the slow waves was reconstructed. In 10 of a total of 66 intestinal segments (15%), a circus movement of the slow wave was detected. These reentries were seen in control ( n = 2) as well as in 3-mo ( n = 2) and 7-mo ( n = 6) diabetic rats. Local conduction velocities and beat-to-beat intervals during the reentries were measured (0.42 ± 0.15 and 3.03 ± 0.67 cm/s, respectively) leading to a wavelength of 1.3 ± 0.5 cm and a circuit diameter of 4.1 ± 1.5 mm. This is the first demonstration of a reentrant arrhythmia in the small intestine of control and diabetic rats. Calculations of the size of the circuits indicate that they are small enough to fit inside the intestinal wall. Extrapolation based on measured velocities and rates indicate that reentrant arrhythmias are also possible in the distal small intestine of larger animals including humans.

scholarly journals A comparative anatomical, histological and histochemical study of small intestine in Kestrel (Falco tunniculus) and white eared bulbul (Picnonotic leucotis) according to their food type

2017 ◽  
Vol 40 (2) ◽  
pp. 36-41
Author(s):  
Ahmed S. AL-A´araji
Keyword(s):  
Small Intestine ◽  
Histological Study ◽  
Goblet Cells ◽  
Food Type ◽  
Intestinal Length ◽  
Intestinal Segments ◽  
Blue Stain ◽  
Neutral Mucin ◽  
Small Intestinal ◽  
Anatomical Part

     This study was conducted on 30 birds (15 birds for each type) divided as 10 birds for each part of study. Anatomical part revealed that the small intestine in both birds kestrel (Falco tinniculus) and white eared bulbul (Picnonotic leucotis) formed from 3 segments; duodenum, jejunum and ileum with no clear demarcation line between them. In kestrel the Meckel's diverticulum appeared as small projection to separate between jejunum and ileum. Both ratio of intestinal length to body length and of intestinal weight to body weight was higher in bulbul than those in kestrel.  Histological study showed that the wall of all three parts of small intestine was composed of the same histological layers; these are mucosa, submucosa, muscularis and serosa. There was almost similarity in structure of these tunics but significant differences in several Histomorphometric measurements of each tunica. Goblet cells were more abundant in all parts of small intestine of bulbul than those in kestrel and there was a gradual increasing in the number of these cells toward the end of intestine of both birds. Histochemical part of this study appeared that in villi and crypts of all small intestinal segments of both birds the goblet cells secrete neutral mucin in nature because it showed negative reaction to Alcian blue stain and positive to PAS stain.

scholarly journals Expression of proposed methionine transporters along the gastrointestinal tract of pigs and their regulation by dietary methionine sources

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Stella Romanet ◽  
Jörg R. Aschenbach ◽  
Robert Pieper ◽  
Jürgen Zentek ◽  
John K. Htoo ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Protein Expression ◽  
Mrna Expression ◽  
Oral Mucosa ◽  
Protein Translation ◽  
Primary Role ◽  
Intestinal Segments ◽  
Small Intestinal

Abstract Background Given the key role of methionine (Met) in biological processes like protein translation, methylation, and antioxidant defense, inadequate Met supply can limit performance. This study investigated the effect of different dietary Met sources on the expression profile of various Met transporters along the gastrointestinal tract (GIT) of pigs. Methods A total of 27 pigs received a diet supplemented with 0.21% DL-Met, 0.21% L-Met, or 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA). Changes in mRNA expression of B0AT1, ATB0,+, rBAT, ASCT2, IMINO, LAT4, y+LAT1, LAT2, and SNAT2 were evaluated in the oral mucosa, cardia, fundus, pylorus, duodenum, proximal jejunum, middle jejunum, ileum, cecum, proximal colon, and distal colon, complemented by protein expression analysis of B0AT1, ASCT2, LAT2, and LAT4. Results Expression of all investigated transcripts differed significantly along the GIT. B0AT1, rBAT, y+LAT1, LAT2, and LAT4 showed strongest mRNA expression in small intestinal segments. ASCT2, IMINO, and SNAT2 were similarly expressed along the small and large intestines but expression differed in the oral mucosa and stomach. ATB0,+ showed highest mRNA expression in large intestinal tissues, cardia, and pylorus. In pigs fed DL-Met, mRNA expression of ASCT2 was higher than in pigs fed DL-HMTBA in small intestinal tissues and mRNA expression of IMINO was lower than in pigs fed L-Met in large intestinal tissues. Dietary DL-HMTBA induced a stronger mRNA expression of basolateral uptake systems either in the small (LAT2) or large (y+LAT1) intestine. Protein expression of B0AT1 was higher in the middle jejunum and ileum in pigs fed DL-Met when compared with the other Met supplements. LAT4 expression was higher in pigs fed DL-HMTBA when compared with DL-Met (small intestine) and L-Met (small intestine, oral mucosa, and stomach). Conclusion A high expression of several Met transporters in small intestinal segments underlines the primary role of these segments in amino acid absorption; however, some Met transporters show high transcript and protein levels also in large intestine, oral mucosa, and stomach. A diet containing DL-Met has potential to increase apical Met transport in the small intestine, whereas a diet containing DL-HMTBA has potential to increase basolateral Met transport in the small intestine and, partly, other gastrointestinal tissues.

Eosinophilic Gastroenteritis Presenting as Acute Intestinal Obstruction

1983 ◽  
Vol 28 (2) ◽  
pp. 183-184 ◽  
Author(s):  
R. J. C. Steele ◽  
R. G. Wright ◽  
H. M. Gilmore
Keyword(s):  
Small Intestine ◽  
Abdominal Pain ◽  
Intestinal Obstruction ◽  
Large Intestine ◽  
Eosinophilic Gastroenteritis ◽  
Acute Intestinal Obstruction ◽  
Clinical Feature ◽  
Distal Small Intestine ◽  
Uncommon Condition ◽  
Small Intestinal

Eosinophilic gastroenteritis is an uncommon condition which usually affects the antrum of the stomach and may occasionally involve the small or large intestine (1). The main clinical feature is usually that of chronic colicky abdominal pain. We report a case presenting as acute small intestinal obstruction due to isolated involvement of the distal small intestine.

Intestinal adaptation during lactation in the mouse. II. Altered intestinal processing of a dietary protein

1993 ◽  
Vol 264 (6) ◽  
pp. G1126-G1132
Author(s):  
P. R. Harmatz ◽  
P. W. Carrington ◽  
V. Giovino-Barry ◽  
R. A. Hatz ◽  
K. J. Bloch
Keyword(s):  
Small Intestine ◽  
Surface Area ◽  
Dietary Protein ◽  
Intestinal Adaptation ◽  
Intestinal Morphology ◽  
Prolonged Contact ◽  
Intestinal Segments ◽  
Wet Weight ◽  
Small Intestinal ◽  
And Control

We previously demonstrated in lactating mice a six- to eightfold increase in the intestinal uptake of the dietary protein, ovalbumin (OVA), administered by gavage. In this study, we tested the possibility that alterations in intestinal morphology, transit time, reduced luminal proteolysis, and enhanced association with the intestinal surface might account for the increased uptake of the protein observed in lactating mice. We found that these animals had a significant increase in length, wet weight, and surface area of the small intestine. No change in the number of Peyer's patches was noted. Intestinal transit was assessed by gavage administration of 125I-OVA and 10 mg OVA and localization of the peak of radioactivity 15, 30, and 60 min after feeding. Although motility (distance traveled per unit time) was not different in lactating and control mice at 15 and 30 min, the fraction of the small intestine traversed by the peak of radioactivity was less in lactating mice. Digestion of 125I-OVA administered by gavage with 10 mg unlabeled OVA was examined by trichloroacetic acid precipitation and gel permeation of the resulting fragments. Lactating and control mice did not show differences in digestion of 125I-OVA by either measurement. The association of 125I-OVA with small intestinal segments, however, was enhanced in lactating mice, especially in the second and third segments of the small intestine. Thus several factors including an increase in length and surface area of the small intestine, prolonged contact of protein with the small intestinal absorptive surface, and enhanced association of the protein with the intestinal surface contribute to increased uptake.(ABSTRACT TRUNCATED AT 250 WORDS)

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