Parastar insecticide induced changes in reproductive parameters and testicular oxidative stress biomarkers in Wistar male rats

2018 ◽  
Vol 34 (7) ◽  
pp. 499-506 ◽  
Author(s):  
Edouard Akono Nantia ◽  
Antoine S Kada ◽  
Faustin PT Manfo ◽  
Nehemiah N Tangu ◽  
Kaghou M Mbifung ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reproductive Function ◽  
Male Rats ◽  
Reproductive Parameters ◽  
Oxidative Stress Biomarkers ◽  
Testosterone Levels ◽  
Stress Biomarkers ◽  
Male Reproductive Function ◽  
Sperm Density ◽  
Wistar Male Rats

Parastar is an insecticide formulation of lambda-cyhalothrin and imidacloprid, and it is largely used for crop production improvement in Santa, North West Region of Cameroon. This study aimed at evaluating the effects of Parastar on reproductive parameters and testicular oxidative stress in adult albino Wistar male rats. Twenty rats (154 g ± 28 g) were divided into four groups of five animals each and treated daily with either distilled water (10 mL/kg), 1.25, 2.49 or 6.23 mg/kg of Parastar, respectively, for 35 days. After treatment, animal reproductive function was evaluated through fertility tests, sperm characteristics, testosterone levels and organ weights, while oxidative stress biomarkers were determined on testicular homogenates. Parastar administration resulted into increased seminal vesicle and prostate weights, while body weight remained unaffected. Parastar dose-dependently reduced sperm density and mobility, and the highest dose decreased serum testosterone levels. Parastar also modulated stress biomarkers with increased thiobarbituric acid reactive substances levels, decreased glutathione levels and inhibition of catalase and superoxide dismutase activities. In conclusion, Parastar negatively affected male reproductive function through alteration of testosterone levels, sperm parameters and induction of oxidative stress in rats.

Associations between oxidative stress biomarkers in different body fluids and reproductive parameters in male partners of subfertile couples

2016 ◽  
Vol 14 (2) ◽  
pp. 46-52
Author(s):  
María Melgarejo ◽  
Jaime Mendiola ◽  
Miriam Moñino-García ◽  
Juan Cabezas-Herrera ◽  
Isabel Tovar-Zapata ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Fluids ◽  
Reproductive Parameters ◽  
Male Partners ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers

scholarly journals Protective Effects of Orlistat on Lipid Profile, Cardiac Oxidative Stress Biomarkers and Histology in High-fat Diet-induced Obese Rats

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Othman ZA ◽  
Wan ghazali WS ◽  
Noordin L ◽  
Omar N ◽  
Mohd. Yusof NA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Male Rats ◽  
Protective Effects ◽  
Sprague Dawley ◽  
High Fat ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Obesity Index ◽  
Obese Rats

Introduction: Orlistat is a widely used drug in treating obesity as it promotes weight reduction. The aim of this study was to determine the protective effects of orlistat (10 mg/kg/day) on cardiovascular parameters and oxidative stress biomarkers in high-fat diet (HFD)-induced obese rats. Methods: Twenty-four male rats Sprague Dawley rats were divided into three groups and fed with normal diet (N), HFD and HFD with orlistat (HFD+O). Orlistat was administered daily by oral gavage and after six weeks, all rats were sacrificed. Results: Administration of orlistat along with HFD (HFD+O) has brought significant decreases in Lee obesity index and LDL level compared to HFD group. Activities of cardiac superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were significantly higher, whereas level of oxidised LDL was significantly lower in HFD+O group compared to HFD group. HFD group had significantly higher necrotic patch area in myocardium while minimal histological changes were seen in HFD+O group. Conclusion: This study may suggest that administration of orlistat at 10 mg/kg/day for 6 weeks may have protective effects against the changes on Lee obesity index, lipid profiles, cardiac oxidative stress biomarkers and histology of myocardium in HFD-induced obese rats possibly through its hypolipidaemic and antioxidant actions.

scholarly journals Silymarin reduced cisplatin-induced hyperalgesia bysuppressing oxidative stress in male rats

2021 ◽  
Vol 25 (2) ◽  
pp. 146-153
Author(s):  
Alireza Mohajjel Nayebi ◽  
◽  
Ali Hashemian ◽  
Hassan Rezazadeh ◽  
Mohammad Charkhpour ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Single Dose ◽  
Antineoplastic Agent ◽  
Total Antioxidant Status ◽  
Male Rats ◽  
Tail Flick ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
The Impact ◽  
And Oxidative Stress

Introduction: Cisplatin is an antineoplastic agent which is used in treatment of various cancers. However its clinical use is associated with oxidative stress-mediated neuropathic pain. This research aimed to explore the effect of silymarin on cisplatin-induced hyperalgesia (CIH) and oxidative stress biomarkers in male rats. Methods: Fifty-six male rats were allocated into seven equal groups. Hyperalgesia was caused by intraperitoneal single dose administration of cisplatin (1mg/kg) and assessed by utilizing tail-flick method. The impact of silymarin (25, 50 and 100 mg/kg/day for 15 days) on CIH was investigated on days 1, 5, 10 and 15. Blood samples were collected to assess malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant status (TAS) on day fifteen. Results: Single dose injection of cisplatin (1mg/kg) could cause a significant hyperalgesia on days 5, 10 and 15. CIH was abolished by daily administration of silymarin (50 and 100mg/kg) on days 10 and 15. Serum MDA level was decreased in cisplatin and silymarin (100 mg/kg) co-treated rats, while there was an increase in GPx, SOD as well as TAS parameters. Conclusion: The results of this study revealed that silymarin prevents from CIH possibly by improving lipid peroxidation and oxidative stress biomarkers. Other clinical studies should be performed to establish possible use of silymarin for treatment of CIH in susceptible individuals.

Oxidative Stress Response in Normal and Antioxidant Supplemented Rats to a Downhill Run: Changes in Blood and Skeletal Muscles

2005 ◽  
Vol 30 (6) ◽  
pp. 677-689 ◽  
Author(s):  
Tongjian You ◽  
Allan H. Goldfarb ◽  
Richard J. Bloomer ◽  
Linh Nguyen ◽  
Xin Sha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscles ◽  
Male Rats ◽  
Protein Carbonyls ◽  
Oxidized Glutathione ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Glutathione Status ◽  
Vastus Intermedius ◽  
Fast Twitch

The purpose of this study was to determine if changes in oxidative stress biomarkers in blood and skeletal muscles are similar in normal and antioxidant supplemented rats after a downhill run. Sixty-six male Sprague-Dawley rats were pretreated with a normal rat diet or diet + antioxidants (2,000 mg vitamin C + 1,000 IU vitamin E/kg diet) for 2 weeks. Exercised rats ran 90 min on a rodent treadmill at a speed of 16 m/min at-16°grade. Rats were sacrificed either at rest, immediately, 2 hrs, or 48 hrs postexercise. Malondialdehyde (MDA) and protein carbonyl (PC) concentrations and glutathione status in blood, vastus lateralis (white fast-twitch), vastus intermedius (red fast-twitch), and soleus (slow-twitch) muscles were determined. A significant increase from rest in PC occurred in plasma, vastus intermedius and soleus muscle 2 hrs after the downhill run (p <  0.05), with no changes observed at any other times postexercise. Antioxidant supplementation significantly decreased PC concentrations in both vastus intermedius and soleus muscles at all times combined (p <  0.05). MDA and glutathione status in blood and muscles were unaffected by either the downhill run or antioxidant treatment. For PC and MDA, the concentrations were lower in blood as compared to skeletal muscle, with the opposite finding for oxidized glutathione; however, the pattern of response postexercise was similar. These data indicate that (a) PC, but not MDA or oxidized glutathione, is elevated transiently following downhill running in male rats; (b) the elevation in PC postexercise occurs in plasma, vastus intermedius, and soleus muscles; (c) antioxidant therapy can attenuate PC in vastus intermedius, and soleus muscles; and (d) while the concentrations of oxidative stress biomarkers differ between blood and the various skeletal muscles, the pattern of response postexercise is similar. Key words: protein carbonyls, lipid peroxidation, eccentric exercise

scholarly journals Administration of Purwoceng (Pimpinella alpina Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

2016 ◽  
Vol 16 (3) ◽  
pp. 115
Author(s):  
Taufiqurrachman Nasihun ◽  
Eni Widayati
Keyword(s):  
Oxidative Stress ◽  
Male Rats ◽  
Aging Male ◽  
Sprague Dawley ◽  
Oxidative Stress Biomarkers ◽  
Frap Assay ◽  
Stress Biomarkers ◽  
Post Hoc ◽  
Stress Biomarker ◽  
Oxidative Stress Biomarker

<strong>Introduction:</strong> UVC may induce oxidative stress mediated by ROS. <em>Pimpinella alpina</em> Molk (PaM) have been utilized by aging male as rejuvenation remedy. <strong>Objective:</strong> To elucidate the PaM effect on oxidative stress biomarker in rats. <strong>Methods:</strong> 25 of Sprague-Dawley male rats were divided into 5 groups, PaM with a dosage of 50, 100, and 150 mg were administered during 15 days to evaluate the biomarker of oxidative stress following UVC irradiation for 30 minutes beforehand. The oxidative stress biomarkers were measured by TBARS and FRAP assay respectively. <strong>Results:</strong> Post Hoc analysis demonstrated that MDA and 8-OHdG concentration in PaM group were significantly lower (p &lt; 0.001), otherwise the concentration of TAC in PaM group were significantly higher, p &lt; 0.001. <strong>Conclusion:</strong> Administration of PaM with 50, 100, and 150 mg dosage capable of improving oxidative stress biomarker in Sprague-Dawley male rats.

The mechanisms involved in obesity-induced male infertility

2020 ◽  
Vol 16 ◽  
Author(s):  
Hamed Heydari ◽  
Rafighe Ghiasi ◽  
Saber Ghaderpour ◽  
Rana Keyhanmanesh
Keyword(s):  
Oxidative Stress ◽  
Metabolic Disease ◽  
Male Infertility ◽  
Male Fertility ◽  
Reproductive Function ◽  
Disease Development ◽  
Significant Evidence ◽  
Male Reproductive Function ◽  
Negative Effect ◽  
And Oxidative Stress

Introduction: Obesity resulted by imbalance between the intake of energy and energy consumption can lead to growth and metabolic disease development in people. Both in obese men and animal models, several studies indicate that obesity leads to male infertility. Objective: This review has discussed some mechanisms involved in obesity-induced male infertility. Method: Online documents were searched through Science Direct, Pubmed, Scopus, and Google Scholar websites dating from 1959 to recognize studies on obesity, kisspeptin, leptin, and infertility. Results: Obesity induced elevated inflammatory cytokines and oxidative stress can affect male reproductive functions including spermatogenesis disorders, reduced male fertility power and hormones involved in hypothalamus-pituitarygonadal axis. Conclusion: There is significant evidence that obesity resulted in male infertility. obesity has negative effect on male reproductive function via several mechanisms such as inflammation and oxidative stress.

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