scholarly journals Protective Effects of Orlistat on Lipid Profile, Cardiac Oxidative Stress Biomarkers and Histology in High-fat Diet-induced Obese Rats

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Othman ZA ◽  
Wan ghazali WS ◽  
Noordin L ◽  
Omar N ◽  
Mohd. Yusof NA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Male Rats ◽  
Protective Effects ◽  
Sprague Dawley ◽  
High Fat ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Obesity Index ◽  
Obese Rats

Introduction: Orlistat is a widely used drug in treating obesity as it promotes weight reduction. The aim of this study was to determine the protective effects of orlistat (10 mg/kg/day) on cardiovascular parameters and oxidative stress biomarkers in high-fat diet (HFD)-induced obese rats. Methods: Twenty-four male rats Sprague Dawley rats were divided into three groups and fed with normal diet (N), HFD and HFD with orlistat (HFD+O). Orlistat was administered daily by oral gavage and after six weeks, all rats were sacrificed. Results: Administration of orlistat along with HFD (HFD+O) has brought significant decreases in Lee obesity index and LDL level compared to HFD group. Activities of cardiac superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were significantly higher, whereas level of oxidised LDL was significantly lower in HFD+O group compared to HFD group. HFD group had significantly higher necrotic patch area in myocardium while minimal histological changes were seen in HFD+O group. Conclusion: This study may suggest that administration of orlistat at 10 mg/kg/day for 6 weeks may have protective effects against the changes on Lee obesity index, lipid profiles, cardiac oxidative stress biomarkers and histology of myocardium in HFD-induced obese rats possibly through its hypolipidaemic and antioxidant actions.

scholarly journals Effects of Vitamin D and Simvastatin on Inflammatory and Oxidative Stress Markers of High-Fat Diet-Induced Obese Rats

2021 ◽  
Vol 2 (3) ◽  
pp. 39-50
Author(s):  
Abdel-Moniem A. Makhlouf ◽  
Atef M. Mahmoud ◽  
Rania G. Ibrahim ◽  
Yasmeen S. Abdel Aziz
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
High Fat Diet ◽  
Male Rats ◽  
Antioxidant Defenses ◽  
Control Group ◽  
High Fat ◽  
Stress Markers ◽  
Obese Rats ◽  
Markers Of Oxidative Stress

Purpose: This study was aimed to evaluate the antioxidant and anti-inflammatory effects of vitamin D and Simvastatin (SIM) on a high-fat diet (HFD) induced-obese rats. Methods: 40 adult male rats were divided into four groups: control group, HFD, HFD + vitamin D, and HFD + SIM for 14 weeks. Vitamin D or SIM supplementation was done for the last 6 weeks. Vitamin D dosage was 500 IU/kg, while SIM dosage was 10 mg/kg. Interleukin-6 (IL-6) concentration and markers of oxidative stress including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione(GSH) concentrations in serum were determined using ELISA kits and spectrophotometry methods, respectively. Results: Treatment with vitamin D or SIM could significantly reduce IL-6 and MDA and increases SOD, GPx activities, and GSH levels. Oxidative stress can result not only from increased ROS production but also from dysfunctional antioxidant defenses. Conclusion: From the experimental results, it was observed that SIM and vitamin D could attenuate oxidative stress and inflammation markers associated with obesity.

Effects of Hypericum Scabrum extract on anxiety and oxidative stress biomarkers in rats fed a long-term high-fat diet

2016 ◽  
Vol 32 (2) ◽  
pp. 503-511 ◽  
Author(s):  
Ahmad Ganji ◽  
Iraj Salehi ◽  
Abdolrahman Sarihi ◽  
Siamak Shahidi ◽  
Alireza Komaki
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
High Fat ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Hypericum Scabrum ◽  
And Oxidative Stress

scholarly journals Protective Effects of Echinacea purpurea Ethanol Extract on Male Reproductive Dysfunction in Obese Rats

2021 ◽  
Vol 11 (5) ◽  
pp. 2392
Author(s):  
Zwe-Ling Kong ◽  
Athira Johnson ◽  
Tzu-Ling Ting ◽  
Po-Jen Cheng ◽  
Chien-Feng Mao
Keyword(s):  
High Fat Diet ◽  
Antioxidant Activities ◽  
Regulatory Protein ◽  
Adenosine Monophosphate ◽  
Echinacea Purpurea ◽  
Peroxisome Proliferator ◽  
Protective Effects ◽  
Sprague Dawley ◽  
High Fat ◽  
Obese Rats

Obesity is characterized by excessive fat accumulation and it may affect reproductive health by altering the sperm parameters, hormonal profiles, and gonadal functions. Echinacea purpurea is a medicinal herb and is well-known for its anti-inflammatory and antioxidant activities. The purpose of this study was to examine the beneficial effects of E. purpurea ethanol (EPE) extract on the high-fat diet (HFD)-induced reproductive damage. The obese condition in male Sprague–Dawley rats was induced by feeding with a high-fat diet (45%) and later three different doses of EPE (93, 186, 465 mg/kg per day) were tested for 5 weeks. The results showed that the level of antioxidant enzymes was increased, whereas lipid peroxidation, blood glucose level, nitric oxide production, IL-6 level, and TNF-α level were decreased in the presence of EPE extract. In addition to this, the sperm counts, motility, morphology, DNA damage, and mitochondrial membrane potential were also improved. Additionally, the protein expression of Peroxisome Proliferator-Activated Receptor Alpha (PPAR-α), and Phospho-Adenosine Monophosphate-Activated Protein Kinase Alpha ½ (AMPKα1/2) phosphorylation in liver tissue and Steroidogenic Acute Regulatory Protein (StAR) and 17β-Hydroxysteroid Dehydrogenase 3 (17β-HSD3) expressions in testes were increased. In conclusion, the administration of EPE extracts beneficially regulated the inflammation and reproductive damage in obese rats.

scholarly journals Anthocyanins from black wolfberry (Lycium ruthenicum Murr.) prevent inflammation and increase fecal fatty acid in diet-induced obese rats

RSC Advances ◽  
2017 ◽  
Vol 7 (75) ◽  
pp. 47848-47853 ◽  
Author(s):  
Jinjin Yin ◽  
Tao Wu
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid ◽  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Lycium Ruthenicum ◽  
Obese Rats ◽  
Lycium Ruthenicum Murr ◽  
Obese Male

This study aimed to determine whether black wolfberry (Lycium ruthenicumMurr.) anthocyanin (BWA) consumption can alleviate oxidative stress and reduce inflammation in high-fat diet-induced obese male Sprague-Dawley rats.

scholarly journals Histidine supplementation alleviates inflammation in the adipose tissue of high-fat diet-induced obese rats via the NF-κB- and PPARγ-involved pathways

2014 ◽  
Vol 112 (4) ◽  
pp. 477-485 ◽  
Author(s):  
Xiaowei Sun ◽  
Rennan Feng ◽  
Yanchuan Li ◽  
Song Lin ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Low Grade ◽  
Obese Women ◽  
Sprague Dawley ◽  
High Fat ◽  
Tnf Α ◽  
Obese Rats ◽  
And Oxidative Stress

Obesity is considered to be accompanied by a chronic low-grade inflammatory state that contributes to the occurrence of many chronic diseases. Our previous study has demonstrated that histidine supplementation significantly ameliorates inflammation and oxidative stress in obese women. However, the in vivo potential mechanisms are not known. The present study was conducted to investigate the mechanisms underlying the effects of histidine on inflammation in a high-fat diet (HFD)-induced female obese rat model. An obese model was established in female Sprague–Dawley rats by HFD feeding for 8 weeks and followed by histidine supplementation for another 4 weeks. The results revealed that HFD-increased body weight and HFD-lowered serum histidine concentrations were significantly reversed by histidine supplementation (P< 0·05). In addition, the serum concentrations of TNF-α, IL-6, C-reactive protein (CRP) and malondialdehyde were significantly reduced and those of superoxide dismutase (SOD) were significantly increased by histidine supplementation when compared with those in obese rats (P< 0·05). Correspondingly, the mRNA expressions of TNF-α, IL-6 and CRP in the adipose tissue were significantly down-regulated and that of CuZnSOD was significantly up-regulated by histidine supplementation (P< 0·05). Histidine supplementation significantly reduced the HFD-induced translocation of NF-κB p65 into the nucleus (P= 0·032) by reducing the phosphorylation of the inhibitor of κBα in the adipose tissue. The results also revealed that the expression of adiponectin was markedly increased both in the serum and in the adipose tissue after histidine supplementation, accompanied by the activation of PPARγ (P= 0·021). These findings indicate that histidine is an effective candidate for ameliorating inflammation and oxidative stress in obese individuals via the NF-κB- and PPARγ-involved pathways.

scholarly journals Protective Effects of Fluvastatin on Reproductive Function in Obese Male Rats Induced by High-Fat Diet through Enhanced Signaling of mTOR

2017 ◽  
Vol 41 (2) ◽  
pp. 598-608 ◽  
Author(s):  
Xiangrong Cui ◽  
Chunlan Long ◽  
Jing Zhu ◽  
Jie Tian
Keyword(s):  
High Fat Diet ◽  
Reproductive Function ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
Protective Effects ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Obese Male

Background: Statins can reduce reproductive damage induced by obesity or high-fat diet (HFD), but the specific regulatory mechanisms are largely unknown. Since mTOR/p70s6k sinaling promotes spermatogonia proliferation and spermatogenesis, we hypothesized that this pathway will be involved in the protective effects of statin in HFD-induced reproductive dysfunction. Methods: Male Sprague Dawley rats (3 weeks old) were randomly divided into a control group (standard diet), HFD group, and a fluvastatin group (HFD + fluvastatin at 6mg/kg, once daily by oral gavage). After 8 weeks, body weight was obtain and rats were sacrificed. Weights of the testes, gross morphology, sperm parameters, circulating levels of sex hormones, lipid levels, and tissue mTOR, p-P70s6k were measured. Another set of male rats were treated with rapamycin or vehicle. Flow cytometry was used to detect the spermatogonia marker c-kit and cell cycle. p-P70s6k expression was analyzed by Western blot. Results: HFD not only results in rat obesity but also leads to spermatogenetic damage and fluvastatin was able to partially block the effects of HFD. Fluvastatin also partially reversed the suppression of mTOR and p-p70s6k expresson. Conclusion: Our data suggest that fluvastatin has protective effects on reproductive function in obese male rats most probably through enhanced signaling of mTOR.

scholarly journals Ameliorative Effect of Spinach on Non-Alcoholic Fatty Liver Disease Induced in Rats by a High-Fat Diet

2019 ◽  
Vol 20 (7) ◽  
pp. 1662 ◽  
Author(s):  
Laura Elvira-Torales ◽  
Gala Martín-Pozuelo ◽  
Rocío González-Barrio ◽  
Inmaculada Navarro-González ◽  
Francisco-José Pallarés ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Fatty Liver ◽  
High Fat Diet ◽  
High Fat ◽  
Oxidative Stress Biomarkers ◽  
Alcoholic Fatty Liver ◽  
Stress Biomarkers ◽  
Expression Of Genes ◽  
And Oxidative Stress

The purpose of this work was to evaluate the effect of dietary carotenoids from spinach on the inflammation and oxidative stress biomarkers, liver lipid profile, and liver transcriptomic and metabolomics profiles in Sprague–Dawley rats with steatosis induced by a high-fat diet. Two concentrations of spinach powder (2.5 and 5%) were used in two types of diet: high-fat (H) and standard (N). Although rats fed diet H showed an accumulation of fat in hepatocytes, they did not show differences in the values of adiponectin, tumor necrosis factor alpha (TNF-α), and oxygen radical absorption (ORAC) in plasma or of isoprostanes in urine compared with animals fed diet N. The consumption of spinach and the accumulation of α and β carotenes and lutein in the liver was inversely correlated with serum total cholesterol and glucose and the content of hepatic cholesterol, increasing monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) and reducing cholesterol in the livers of rats fed diet H and spinach. In addition, changes in the expression of genes related to the fatty liver condition occurred, and the expression of genes involved in the metabolism of fatty acids and cholesterol increased, mainly through the overexpression of peroxisome proliferator activated receptors (PPARs). Related to liver metabolites, animals fed with diet H showed hypoaminoacidemia, mainly for the glucogenic aminoacids. Although no changes were observed in inflammation and oxidative stress biomarkers, the consumption of spinach modulated the lipid metabolism in liver, which must be taken into consideration during the dietary treatment of steatosis.

scholarly journals Administration of Purwoceng (Pimpinella alpina Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Spargue-Dawley Male Rats

2016 ◽  
Vol 16 (3) ◽  
pp. 115
Author(s):  
Taufiqurrachman Nasihun ◽  
Eni Widayati
Keyword(s):  
Oxidative Stress ◽  
Male Rats ◽  
Aging Male ◽  
Sprague Dawley ◽  
Oxidative Stress Biomarkers ◽  
Frap Assay ◽  
Stress Biomarkers ◽  
Post Hoc ◽  
Stress Biomarker ◽  
Oxidative Stress Biomarker

<strong>Introduction:</strong> UVC may induce oxidative stress mediated by ROS. <em>Pimpinella alpina</em> Molk (PaM) have been utilized by aging male as rejuvenation remedy. <strong>Objective:</strong> To elucidate the PaM effect on oxidative stress biomarker in rats. <strong>Methods:</strong> 25 of Sprague-Dawley male rats were divided into 5 groups, PaM with a dosage of 50, 100, and 150 mg were administered during 15 days to evaluate the biomarker of oxidative stress following UVC irradiation for 30 minutes beforehand. The oxidative stress biomarkers were measured by TBARS and FRAP assay respectively. <strong>Results:</strong> Post Hoc analysis demonstrated that MDA and 8-OHdG concentration in PaM group were significantly lower (p &lt; 0.001), otherwise the concentration of TAC in PaM group were significantly higher, p &lt; 0.001. <strong>Conclusion:</strong> Administration of PaM with 50, 100, and 150 mg dosage capable of improving oxidative stress biomarker in Sprague-Dawley male rats.

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