The Association of Human Papillomavirus Infection with Balanoposthitis: A Description of Five Cases with Proposals for Treatment

1994 ◽  
Vol 5 (2) ◽  
pp. 139-141 ◽  
Author(s):  
H D L Birley ◽  
G A Luzzi ◽  
M M Walker ◽  
B Ryait ◽  
D Taylor-Robinson ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Hpv Infection ◽  
Topical Steroids ◽  
Treatment Needs ◽  
Hpv Dna ◽  
Papillomavirus Infection ◽  
Oral Isotretinoin ◽  
Polymerase Chain

The features and clinical course of chronic balanitis in 5 patients are presented. In each case, histological examination of a cutaneous biopsy sample showed pronounced features of human papillomavirus (HPV) infection. In addition, HPV DNA was demonstrated in each biopsy specimen by a polymerase chain reaction and was found to be type 6 by Southern blot hybridization in 4 of the cases. Although the association of histological features of HPV infection with balanitis does not prove that HPV is causal, the failure to find other causes, the prolonged and distressing symptoms, and the ineffectiveness of topical steroids in improving symptoms, all suggest the importance of HPV infection. While effective treatment needs to be sought and developed, the response of one patient to oral isotretinoin suggests that this agent may be appropriate for a larger trial.

Verucciform Xanthoma of the Penis Not Associated With Human Papillomavirus Infection

2005 ◽  
Vol 129 (3) ◽  
pp. e62-e64 ◽  
Author(s):  
Çağatay Erşahin ◽  
Anna M. Szpaderska ◽  
Kimberly Foreman ◽  
Sherri Yong
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Nested Polymerase Chain Reaction ◽  
Hpv Dna ◽  
Negative Results ◽  
Papillomavirus Infection ◽  
Rare Lesion ◽  
Hpv Types ◽  
Polymerase Chain ◽  
Primer Sets

Abstract Verruciform xanthoma (VX) is a rare lesion with a predilection for oral mucosa. Only 16 cases of VX of the penis have been reported. Histologically, VX lesions in different locations are identical; however, the etiology is controversial. Previous studies have reported the presence of human papillomavirus (HPV) in VX of the skin. The purpose of this study was to determine whether HPV is a causative agent in this rare case of VX of the penis. Microscopically, the lesion demonstrated prominent verrucoid squamous hyperplasia with hyperkeratosis, parakeratosis, and acanthosis. Histiocytes, a hallmark of VX, were identified in the elongated dermal papillae. Nested polymerase chain reaction was performed on the DNA with the commonly used primer sets MY9/MY11 and GP5+/GP6+, which identify more than 40 HPV types. The results failed to identify HPV DNA in the sample, although HPV could be readily detected in genomic DNA extracted from paraffin-embedded condyloma acuminatum, a known HPV-associated lesion. Additionally, we tested a VX lesion of the palate for HPV DNA and obtained negative results. Our results indicate that VX can arise without HPV infection and suggest other possible origins may be involved.

Detection of Human Papillomavirus Infection in Diseased and Nondiseased Sites of the Respiratory Tract in Recurrent Respiratory Papillomatosis Patients by DNA Hybridization

1992 ◽  
Vol 101 (5) ◽  
pp. 408-412 ◽  
Author(s):  
Shirley Pignatari ◽  
Elaine M. Smith ◽  
Cynthia Shive ◽  
Steven D. Gray ◽  
Lubomir P. Turek
Keyword(s):  
Human Papillomavirus ◽  
Respiratory Tract ◽  
Dna Hybridization ◽  
Hpv Infection ◽  
Recurrent Respiratory Papillomatosis ◽  
Hpv Dna ◽  
Clinically Normal ◽  
Papillomavirus Infection ◽  
Hybridization Procedure ◽  
Cervical Trachea

We examined 12 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their disease, for the presence of human papillomavirus (HPV). Biopsies were obtained from their respiratory papillomas and nondiseased sites (NDS) of the respiratory tract: the nasopharynx, posterior tonsillar pillar, aryepiglottic fold, cervical trachea, intrathoracic trachea, and bronchi. The presence of HPV DNA was determined by using the ViraPap/ViraType DNA hybridization procedure. Two thirds of the patients were infected with HPV 6/11 in either the diseased papilloma or NDS: 50% of patients with papilloma specimens typed positive (6 of 12), and 40% of the patients typed HPV-positive in one or more biopsies from the NDS (4 of 10). No single NDS was more likely to be infected with HPV than any other. No oncogenic HPV types 16/18 or 31/33/35 were detected in the papillomas or NDS of these patients. Only patients with multiple, and not isolated, papilloma involvement were found to harbor HPV DNA in NDS; and 80 % of those with infected NDS, compared to 20 % of those without infected NDS, required a shorter (no more than 3 months) surgical treatment interval. Our results show that HPV infection frequently persists in adjacent, clinically normal sites, and suggest that the extent of NDS involvement may predict both the extent of disease and the likelihood of recurrence.

scholarly journals The Prevalence of Human Papillomavirus between the Neonates and Their Mothers

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Mariusz Skoczyński ◽  
Anna Goździcka-Józefiak ◽  
Anna Kwaśniewska
Keyword(s):  
Human Papillomavirus ◽  
Pregnant Women ◽  
Significant Risk ◽  
Hpv Infection ◽  
Hormonal Status ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Polymerase Chain ◽  
Medical University ◽  
The Impact

The impact of human papillomavirus (HPV) infection on pregnancy is a major problem of medicine. The transmission of the virus from mother to fetus is a process yet unresolved. The immune response and changed hormonal status of pregnant women might facilitate infection. A research on the prevalence of HPV infection was conducted at the Clinic of Obstetrics, Medical University of Lublin (Poland). The studied group included 152 randomly selected women. The material was tested for the presence of HPV DNA by means of polymerase chain reaction (PCR). The aim of the research was to assess the relation between HPV infections detected in the buccal smears of the neonates and the incidence of such infections in the cervical/buccal smears of their mothers. In the group of 152 infants HPV was found in 16 (10.53%). Among the cervical/buccal smears, HPV was isolated, respectively, in 24 (15.79%) and in 19 (12.5%) pregnant women. Statistically significant differences in the prevalence of HPV swabs from the newborns and the cervical/buccal smears of their mothers were found (p< 0.001). The identification of mothers in whose buccal smears HPV was detected can help develop a group of children who run a relatively significant risk of being infected.

Pitfalls in the epidemiologic classification of human papillomavirus types associated with cervical cancer using polymerase chain reaction: driver and passenger

2008 ◽  
Vol 18 (5) ◽  
pp. 1042-1050 ◽  
Author(s):  
T. Matsukura ◽  
M. Sugase
Keyword(s):  
Cervical Cancer ◽  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Large Scale ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Cervical Lesions ◽  
Chain Reaction ◽  
Polymerase Chain

Cervical cancer is a common malignancy in women worldwide, and it has now been established that the human papillomavirus (HPV) is both necessary and causal for these lesions. HPV itself is both ubiquitous and markedly heterogeneous but can nevertheless be classified as either a high-risk type or a low-risk type based upon its frequency of detection in cervical cancer. Given that the association between HPV and cervical cancer is causal, the classification of this virus has been strengthened by large-scale epidemiologic studies and is widely accepted across many disciplines. It is evident, however, that cervical cancer is frequently associated with multiple HPV types. Therefore, it is crucial to distinguish causal types of HPV (drivers) from noncausal types (passengers) in cervical lesions. In this review, we highlight the current pitfalls of using polymerase chain reaction methods instead of Southern blot hybridization for detecting HPV and discuss the distinction between driver and passenger HPVs with regard to the viral type, the length of the viral genome, and the levels of viral DNA associated with cervical cancer. Finally, we newly propose three categories of HPV instead of two risk groups, based on similarities between viral genes

Human Papillomavirus (HPV) Cervical Lesions: Results from 300 Italian Women Studied with Dna Hybridization Techniques and Morphology

1988 ◽  
Vol 74 (6) ◽  
pp. 745-749 ◽  
Author(s):  
Fiorella Nuzzo ◽  
Vittorio Tison ◽  
Antonella Castagnoli ◽  
Maurizio Tiboni ◽  
Ethel-Michele De Villiers ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Southern Blot ◽  
Dna Hybridization ◽  
Blot Hybridization ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cervical Infection

Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HPV-DNA in cervical biopsy specimens). The prevalence of HPV cervical infection diagnosed by PAP smears was 11.6 %. Despite disadvantages, filter in situ hybridization was confirmed to be particularly useful for screening purposes to detect HPV in cervical scrapings. In 3 cases it was the only applicable method for diagnosing « high-risk » HPV infection. Southern blot hybridization of tissue DNA with HPV 16-DNA revealed the presence of this virus in 8 cases, and HPV 31-DNA and HPV 42-DNA in 1 case each.

scholarly journals Human papillomavirus infection in nasal polyps in a Chinese population

2011 ◽  
Vol 92 (8) ◽  
pp. 1795-1799 ◽  
Author(s):  
Fei Pei ◽  
Xiao-Ping Chen ◽  
Yi Zhang ◽  
Yan Wang ◽  
Qun Chen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nasal Polyps ◽  
Hpv Infection ◽  
Clinicopathological Characteristics ◽  
Low Risk ◽  
Future Research ◽  
Genotype Distribution ◽  
Healthy Controls ◽  
Hpv Dna ◽  
Papillomavirus Infection

In order to determine the prevalence and genotype distribution of human papillomavirus (HPV) infection in patients with nasal polyps, a total of 204 patients with nasal polyps and 36 healthy controls were recruited for this study. Genomic DNA was extracted from paraffin-embedded tissue sections. HPV DNA genotyping was achieved by a flow-through hybridization and gene-chip method. HPV-positive infection was identified in 82 of 204 (40.2 %) patients, while HPV DNA was not found in healthy controls (P<0.05). Genotyping analysis showed that low-risk HPV genotype 11 was the most prevalent type of HPV in nasal polyps (45.28 %). Both single and multiple HPV genotype infections were found in these HPV-positive cases, although most (74.39 %) were infected with a single genotype. In addition, there was no correlation between HPV infection or HPV subtypes and the clinicopathological characteristics of patients, such as age, gender, number of surgery and disease course. The data from our study clearly demonstrated that HPV infection was associated with nasal polyps. Both high-risk HPV and low-risk HPV (LR-HPV) genotypes were identified in nasal polyp tissues, and LR-HPV-11 was the most prevalent type. Future research will explore the association of HPV infection with the development and progression of nasal polyps.

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