Antiproliferation and anti-migration induced by gypenosides in human colon cancer SW620 and esophageal cancer Eca-109 cells

Gypenosides (Gyp), found in Gynostemma pentaphyllum Makino, has attracted more attention owing to its wide bioactivities. However, the effects of Gyp on esophageal cancer cells and colon cancer cells are still unknown. The present study was to investigate the possible anti-proliferative and anti-migration activity of Gyp on human colon cancer cells SW620 and esophageal cancer cells Eca-109. Cell viability was evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell membrane integrity was evaluated using flow cytometry following propidium iodide staining. Apoptotic cell death was determined by nuclear 4′-6-diamidino-2-phenylindole staining. Generation of intracellular reactive oxygen species (ROS) and changes in mitochondrial membrane potential (Δ ψm) was analyzed by flow cytometry using 2′,7′-dichlorofluorescein–diacetate and rhodamine 123 staining, respectively. Wound healing assay was carried out to investigate Gyp-inhibited migration of SW620 and Eca-109 cells. The results indicated that Gyp inhibited cell proliferation and migration in SW620 and Eca-109 cells in dose- and time-dependent manner. Gyp elevated intracellular ROS level, decreased the Δ ψm, and induced apoptotic morphology such as cell shrinkage and chromatin condensation, suggesting oxidative stress and mitochondria-dependent cell apoptosis that might be involved in Gyp-induced cell viability loss in SW620 and Eca-109 cells. The findings indicate Gyp may have valuable application in clinical colon cancer and esophageal cancer treatments.

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Chk1 and DNA-PK mediate TPEN-induced DNA damage in a ROS dependent manner in human colon cancer cells

Cancer Biology & Therapy ◽

10.1080/15384047.2016.1235658 ◽

2016 ◽

Vol 17 (11) ◽

pp. 1139-1148 ◽

Cited By ~ 9

Author(s):

Omar Nasser Rahal ◽

Maamoun Fatfat ◽

Carla Hankache ◽

Bassam Osman ◽

Hala Khalife ◽

...

Keyword(s):

Colon Cancer ◽

Dna Damage ◽

Cancer Cells ◽

Human Colon ◽

Dependent Manner ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Human Colon Cancer Cells

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Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells

Scientific Reports ◽

10.1038/s41598-021-84970-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Heping Cao ◽

Kandan Sethumadhavan ◽

Fangping Cao ◽

Thomas T. Y. Wang

Keyword(s):

Colon Cancer ◽

Cell Viability ◽

Cancer Cells ◽

Molecular Mechanisms ◽

Human Colon ◽

Mrna Levels ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Number Of Genes ◽

Human Colon Cancer Cells

AbstractPlant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene expression in cancer cells. In this study, human colon cancer cells (COLO 225) were treated with gossypol. MTT assay showed significant inhibitory effect under high concentration and longtime treatment. We analyzed the expression of 55 genes at the mRNA level in the cells; many of them are regulated by gossypol or ZFP36/TTP in cancer cells. BCL2 mRNA was the most stable among the 55 mRNAs analyzed in human colon cancer cells. GAPDH and RPL32 mRNAs were not good qPCR references for the colon cancer cells. Gossypol decreased the mRNA levels of DGAT, GLUT, TTP, IL families and a number of previously reported genes. In particular, gossypol suppressed the expression of genes coding for CLAUDIN1, ELK1, FAS, GAPDH, IL2, IL8 and ZFAND5 mRNAs, but enhanced the expression of the gene coding for GLUT3 mRNA. The results showed that gossypol inhibited cell survival with decreased expression of a number of genes in the colon cancer cells.

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Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) fromTaiwanofungus camphoratus

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/750230 ◽

2011 ◽

Vol 2011 ◽

pp. 1-13 ◽

Cited By ~ 14

Author(s):

Chi-Tai Yeh ◽

Chih-Jung Yao ◽

Jiann-Long Yan ◽

Shuang-En Chuang ◽

Liang-Ming Lee ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Human Colon ◽

Dependent Manner ◽

Apoptosis Induction ◽

Colon Cancer Cells ◽

Active Fraction ◽

Human Colon Cancer ◽

Taiwanofungus Camphoratus ◽

Human Colon Cancer Cells

Aberrant activation of Wnt/β-catenin signaling plays an important role in the development of colon cancer. HS7 is an active fraction extracted fromTaiwanofungus camphoratus, which had been widely used as complementary medicine for Taiwan cancer patients in the past decades. In this study, we demonstrated the effects of HS7 on the growth inhibition, apoptosis induction, and Wnt/β-catenin signaling suppression in human colon cancer cells. HS7 significantly inhibited proliferation of HT29, HCT116, and SW480 colon cancer cells in a dose- and time-dependent manner. The apoptosis induction was evidenced by DNA fragmentation and subG1 accumulation, which was associated with increased Bax/Bcl-2 ratio, activation of caspase-3 and cleavage of PARP. By using Tcf-dependent luciferase activity assay, HS7 was found to inhibit theβ-catenin/Tcf transcriptional activities. In addition, HS7 strongly suppressed the binding of Tcf complexes to its DNA-binding site shown in electrophoretic mobility shift assay. This inhibition was further confirmed by the decreased protein levels of Tcf-4 andβ-catenin. Theβ-catenin/Tcf downstream target genes, such assurvivin,c-myc,cyclin D1,MMP7, andMT1-MMPinvolved in apoptosis, invasion, and angiogenesis were also diminished as well. These results indicate thatTaiwanofungus camphoratusmay provide a benefit as integrative medicine for the treatment of colon cancer.

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Regulation of CYP24 splicing by 1,25-dihydroxyvitamin D3 in human colon cancer cells

Journal of Endocrinology ◽

10.1530/joe-11-0305 ◽

2011 ◽

Vol 212 (2) ◽

pp. 207-215 ◽

Cited By ~ 8

Author(s):

Xinjian Peng ◽

Nishant Tiwari ◽

Sarbani Roy ◽

Liang Yuan ◽

Genoveva Murillo ◽

...

Keyword(s):

Colon Cancer ◽

Vitamin D ◽

Cancer Cells ◽

Rna Splicing ◽

Human Colon ◽

Dependent Manner ◽

Colon Cancer Cells ◽

Selective Treatment ◽

Human Colon Cancer ◽

Dihydroxyvitamin D3

CYP24is a well-established vitamin D receptor (VDR) target gene. The active VDR ligand 1,25(OH)2D3regulates its own catabolism by increasingCYP24expression. It is well known that in the presence of 1,25(OH)2D3, VDR binds to VDREs in the promoter region ofCYP24and initiatesCYP24transcription. However, little is known about the role of 1,25(OH)2D3in the posttranscriptional modulation ofCYP24. In this study, we investigated the functional significance of 1,25(OH)2D3inCYP24RNA splicing in colon cancer cells. Using RT-PCR, we found that 1,25(OH)2D3actively inducesCYP24splicing in a time-dependent manner andCYP24splicing pattern could be cell type or tissue specific. The induction of RNA splicing by 1,25(OH)2D3was mainlyCYP24selective. Treatment of cells with parathyroid hormone inhibited basalCYP24splicing, but failed to inhibit 1,25(OH)2D3-inducedCYP24splicing. Further experiments demonstrated that new RNA synthesis was required for the induction ofCYP24splicing by vitamin D. In addition, alteration of multiple signaling pathways also affectedCYP24splicing and cellular sensitivity in response to vitamin D appeared to correlate with the induction ofCYP24splicing. These results suggest that 1,25(OH)2D3not only regulates CYP24transcription, but also plays an important role in posttranscriptional modulation ofCYP24by inducing its splicing. Our findings reveal an additional regulatory step that makes the vitamin D mediated action more prompt and efficient.

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Zedoary Turmeric Oil Induces Senescence and Apoptosis in Human Colon Cancer HCT116 Cells

Natural Product Communications ◽

10.1177/1934578x1801300731 ◽

2018 ◽

Vol 13 (7) ◽

pp. 1934578X1801300

Author(s):

Meng-Qi Su ◽

Yi-Ran Zhou ◽

Cheng-Qin Li ◽

Zhou Wang ◽

Yue-Liang Wang ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Western Blotting ◽

Human Colon ◽

Dependent Manner ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Related Proteins ◽

Human Colon Cancer Cells ◽

Hct116 Cells

Zedoary turmeric oil (ZTO) is a volatile oil that is extracted from the dry rhizome of Curcuma zedoaria with a variety of biological activities, including anti-tumor activity. However, there is a lack of knowledge about the effect and mechanism of ZTO in human colon cancer cells. The aim of this study was to examine the potential efficacy of ZTO against human colon cancer cells (HCT116) and to uncover the molecular mechanisms of its anti-tumor effects. The anti-proliferative activity of ZTO was determined by the MTT assay, cell counts and colony formation assay. Senescent cells were detected using SA-β-Gal staining, while apoptosis and the CD44+ subpopulation were evaluated by flow cytometry. The expression levels of senescence- and apoptosis-related proteins were examined using western blotting. The results showed that treatment with ZTO significantly inhibited the growth of HCT116 cells and caused senescence and apoptosis in a dose- and time-dependent manner. Western blotting revealed that ZTO significantly increased the expression of senescence- and apoptosis-related proteins p16, p21, and p53 and the phosphorylation of ERK. Moreover, ZTO treatment reduced the cancer stem-like CD44 positive cell population. These findings suggest that ZTO inhibits human colon cancer cells by inducing senescence and apoptosis.

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Corrigendum to “Association of Ozone with 5-Fluorouracil and Cisplatin in Regulation of Human Colon Cancer Cell Viability: In Vitro Anti-Inflammatory Properties of Ozone in Colon Cancer Cells Exposed to Lipopolysaccharides”

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/3297906 ◽

2018 ◽

Vol 2018 ◽

pp. 1-2 ◽

Cited By ~ 1

Author(s):

Vincenzo Simonetti ◽

Vincenzo Quagliariello ◽

Pierangela Giustetto ◽

Marianno Franzini ◽

Rosario Vincenzo Iaffaioli

Keyword(s):

Colon Cancer ◽

Cell Viability ◽

Cancer Cells ◽

Cancer Cell ◽

Human Colon ◽

Colon Cancer Cell ◽

Human Colon Cancer Cell ◽

Colon Cancer Cells ◽

Human Colon Cancer

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An in vitro investigation of photodynamic efficacy of FosPegⓇ on human colon cancer cells

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545815500273 ◽

2015 ◽

Vol 08 (05) ◽

pp. 1550027 ◽

Cited By ~ 4

Author(s):

Ricky W. K. Wu ◽

Ellie S. M. Chu ◽

Zheng Huang ◽

Malini C. Olivo ◽

David C. W. Ip ◽

...

Keyword(s):

Colon Cancer ◽

Cell Death ◽

Cancer Cells ◽

Human Colon ◽

Light Dose ◽

Dependent Manner ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Human Colon Cancer Cells

Photodynamic therapy (PDT) is a novel therapeutic approach for combating various cancers. PDT involves the administration of a photosensitizer which generates singlet oxygen after light activation. FosPegⓇ is the liposomal formulation of mTHPC. In this in vitro study, the photodynamic efficacy of FosPegⓇ on a human colon cancer cell line (HT29) was investigated via studying the cellular uptake of FosPegⓇ, FosPegⓇ PDT mediated photocytotoxicity and the cell death mechanism were triggered. FosPegⓇ PDT demonstrated its antitumor effect in a drug and light dose-dependent manner in HT-29 cells. Lethal dose (LD50) was achieved with 0.4 μg/mL of drug and 3 J/cm-2 of light dose. FosPegⓇ PDT triggered apoptotic cell death via activating caspase cascade and regulating cell cycle progression. In conclusion, FosPegⓇ-PDT is an effective measure to combat human colon cancer cells.

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