Epstein-Barr Virus in Nasopharyngeal Carcinoma of Guatemalan and Brazilian Patients

2016 ◽  
Vol 25 (4) ◽  
pp. 304-309 ◽  
Author(s):  
Alicia Rumayor Piña ◽  
Harim Tavares dos Santos ◽  
Roman Carlos ◽  
Albina Altemani ◽  
Oslei Paes de Almeida
Keyword(s):  
In Situ Hybridization ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Background. Nasopharyngeal carcinoma (NPC) is highly associated with Epstein-Barr virus (EBV), particularly the undifferentiated nonkeratinizing subtype. Prevalence of EBV in NPC in countries such as Guatemala and Brazil has not been studied. Methods. We analyzed 19 cases of NPC, 11 from Guatemala and 8 from Brazil, for the presence of EBV by in situ hybridization and immunohistochemistry. Additionally, 19 hyperplastic adenoids from children were analyzed for EBV by in situ hybridization, 12 from Guatemala and 7 from Brazil. Results. All the NPC cases from Guatemala and 5 from Brazil were of the undifferentiated nonkeratinizing type. EBV-negative cases comprised 2 keratinizing NPC and 1 differentiated nonkeratinizing NPC. All undifferentiated nonkeratinizing NPC from both samples showed intense positivity for EBER, while LMP-1 only focally and scarcely expressed. EBER was positive in 75% and 43% of the adenoids from Guatemala and Brazil, respectively. Conclusions. All undifferentiated nonkeratinizing NPC irrespective of origin from Guatemala or Brazil were highly associated with EBV.

Analysis of Prognostic Factors of Nasopharyngeal Carcinoma: Impact of in Situ Hybridization for Epstein-Barr Virus Encoded Small RNA 1

2006 ◽  
Vol 134 (4) ◽  
pp. 639-645 ◽  
Author(s):  
Kazunari Nakao ◽  
Masato Mochiki ◽  
Ken-Ichi Nibu ◽  
Masashi Sugasawa ◽  
Hiroshi Uozaki
Keyword(s):  
Prognostic Factors ◽  
In Situ Hybridization ◽  
Nasopharyngeal Carcinoma ◽  
Small Rna ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Detection of Epstein-Barr Virus in Nasopharyngeal Carcinoma by In Situ Hybridization and Polymerase Chain Reaction

1997 ◽  
Vol 107 (4) ◽  
pp. 523-526 ◽  
Author(s):  
Shigeyuki Murono ◽  
Tomokazu Yoshizaki ◽  
Saichiro Tanaka ◽  
Hajime Takeshita ◽  
Chang-Soo Park ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

scholarly journals Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection

2012 ◽  
Vol 43 (1) ◽  
pp. 393-404 ◽  
Author(s):  
Marcos Antonio Pereira de Lima ◽  
Márcia Valéria Pitombeira Ferreira ◽  
Marcos Aurélio Pessoa Barros ◽  
Maria Inês de Moura Campos Pardini ◽  
Adriana Camargo Ferrasi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

The impact of Epstein-Barr virus status on clinical outcome in diffuse large B-cell lymphoma

Blood ◽  
2007 ◽  
Vol 110 (3) ◽  
pp. 972-978 ◽  
Author(s):  
Sarah Park ◽  
Jeeyun Lee ◽  
Young Hyeh Ko ◽  
Arum Han ◽  
Hyun Jung Jun ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
B Cell ◽  
Epstein Barr Virus ◽  
Progression Free Survival ◽  
B Cell Lymphoma ◽  
Free Survival ◽  
Barr Virus ◽  
Large B Cell Lymphoma ◽  
Epstein Barr

AbstractTo define prognostic impact of Epstein-Barr virus (EBV) infection in diffuse large B-cell lymphoma (DLBCL), we investigated EBV status in patients with DLBCL. In all, 380 slides from paraffin-embedded tissue were available for analysis by EBV-encoded RNA-1 (EBER) in situ hybridization, and 34 cases (9.0%) were identified as EBER-positive. EBER positivity was significantly associated with age greater than 60 years (P = .005), more advanced stage (P < .001), more than one extranodal involvement (P = .009), higher International Prognostic Index (IPI) risk group (P = .015), presence of B symptom (P = .004), and poorer outcome to initial treatment (P = .006). The EBER+ patients with DLBCL demonstrated substantially poorer overall survival (EBER+ vs EBER− 35.8 months [95% confidence interval (CI), 0-114.1 months] vs not reached, P = .026) and progression-free survival (EBER+ vs EBER− 12.8 months [95% CI, 0-31.8 months] vs 35.8 months [95% CI, 0-114.1 months], respectively (P = .018). In nongerminal center B-cell–like subtype, EBER in situ hybridization positivity retained its statistical significance at the multivariate level (P = .045). Nongerminal center B-cell–like patients with DLBCL with EBER positivity showed substantially poorer overall survival with 2.9-fold (95% CI, 1.1-8.1) risk for death. Taken together, DLBCL patients with EBER in situ hybridization+ pursued more rapidly deteriorating clinical course with poorer treatment response, survival, and progression-free survival.

Detection of Epstein–Barr virus in children's adenoids by in situ hybridization

2003 ◽  
Vol 1257 ◽  
pp. 157-160 ◽  
Author(s):  
L.H. Endo ◽  
E. Sakano ◽  
L.A. Camargo ◽  
D.R. Ferreira ◽  
G.A. Pinto ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr
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