scholarly journals Increased Soluble Fibrin in Plasma of Patients with Disseminated Intravascular Coagulation

2003 ◽  
Vol 9 (3) ◽  
pp. 233-240 ◽  
Author(s):  
Hideo Wada ◽  
Tomohiro Sase ◽  
Takeshi Matsumoto ◽  
Fumihiko Kushiya ◽  
Miho Sakakura ◽  
...  
Keyword(s):  
Disseminated Intravascular Coagulation ◽  
Plasma Levels ◽  
Operating Characteristic ◽  
Degradation Products ◽  
Characteristic Analysis ◽  
Soluble Fibrin ◽  
Intravascular Coagulation ◽  
Hemostatic Markers ◽  
Plasmin Inhibitor

Plasma levels of soluble fibrin (SF) were measured in 1184 patients with disseminated intravascular coagulation (DIC) according to Japanese Ministry of Health and Welfare (JMHW) criteria. The usefulness of SF for the diagnosis of DIC was compared with other hemostatic molecular markers. Most hemostatic markers were significantly increased in patients with DIC than in those without DIC. Plasma levels of fibrin and fibrinogen degradation products, thrombin-antihtrombin complex, plasmin-plasmin inhibitor complex, D-dimer, thrombomodulin, and SF levels were also significantly higher in those with pre-DIC than in those without DIC. In classification of overt DIC by International Society of Thrombosis and Haemostasis (ISTH) criteria, most hemostatic markers were significantly increased in patients with overt DIC than in those without overt DIC. Plasma levels of SF 'in patients with DIC were significantly higher than those in patients with pre-DIC, which were significantly higher than in those without DIC. Plasma levels of SF were also significantly higher in patients with overt DIC than in those with non-overt DIC. The correlation between plasma SF levels and DIC score according to JMHW criteria or ISTH criteria was good. Receiver operating characteristic analysis shows that SF was the best marker for the diagnosis of DIC or overt DIC. These findings suggest that plasma SF might be useful marker for the diagnosis of DIC or overt DIC.

Measurement of soluble fibrin monomer-fibrinogen complex in plasmas derived from patients with various underlying clinical situations

2003 ◽  
Vol 89 (05) ◽  
pp. 832-836 ◽  
Author(s):  
Yumiko Kazahaya ◽  
Yuichi Shintani ◽  
Kensuke Yamazumi ◽  
Yutaka Eguchi ◽  
Shin Koga ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disseminated Intravascular Coagulation ◽  
Degradation Products ◽  
Research Society ◽  
Distinct Entity ◽  
Soluble Fibrin ◽  
Intravascular Coagulation ◽  
Fibrin Degradation Products ◽  
Fibrin Monomer ◽  
D Dimer

SummaryWe previously reported a monoclonal antibody named IF-43 that specifically recognizes thrombin-modified fibrinogen (desAA- and desAABB- fibrin monomer) bound with fibrinogen or other D1 domain-containing plasmic fragments such as fragments X, Y, and D1, but not intact fibrinogen or cross-linked fibrin degradation products (XDP). Here, we tentatively named such complexes, soluble fibrin monomer (FM) -fibrinogen complex.By utilizing IF-43, we have developed a kit to measure soluble FM-fibrinogen complex and compared the profiles with those of two established molecular markers for thrombo-embolic disorders: i.e. the thrombin-antithrombin complex (TAT) and the D-dimer in plasma of patients who underwent surgery without any thrombo-embolic complications. The result indicated that soluble FM-fibrinogen complex is a distinct entity from the two established molecular markers. We have also attempted to observe their profiles in patients with the disseminated intravascular coagulation syndrome (DIC). Although the profiles of soluble FM-fibrinogen complex in individual patients appeared to vary from one patient to the other, the plasma level of soluble FM-fibrinogen complex was found to be increased at the initial phase of disseminated intravascular coagulation syndrome. Thus, the soluble FM-fibrinogen complex may serve as an independent molecular marker for the detection of thrombin generation and the diagnosis of thrombosis. The soluble FM-fibrinogen complex may also serve as a risk factor for thrombosis, because it may precipitate as insoluble complexes beyond its threshold in plasma, or when it is modified by thrombin.Part of this paper was originally presented at the 17th International Fibrinogen Workshop of the International Fibrinogen Research Society (IFRS) held in Munich, Germany, September, 2002.

scholarly journals A Pathological Clarification of Sepsis-Associated Disseminated Intravascular Coagulation Based on Comprehensive Coagulation and Fibrinolysis Function

2020 ◽  
Vol 120 (09) ◽  
pp. 1257-1269 ◽  
Author(s):  
Tomoko Onishi ◽  
Keiji Nogami ◽  
Takashi Ishihara ◽  
Satoki Inoue ◽  
Masahiko Kawaguchi ◽  
...  
Keyword(s):  
Disseminated Intravascular Coagulation ◽  
Degradation Products ◽  
Waveform Analysis ◽  
Peak Ratio ◽  
Intravascular Coagulation ◽  
Fibrin Degradation Products ◽  
Functional Dynamics ◽  
Underlying Diseases ◽  
Plasmin Inhibitor ◽  
Coagulation And Fibrinolysis

Abstract Background The functional dynamics of coagulation and fibrinolysis in patients with disseminated intravascular coagulation (DIC) vary due to the pathology and severity of various underlying diseases. Conventional measurements of hemostasis such as thrombin–antithrombin complex, plasmin-α2-plasmin-inhibitor complex, and fibrinogen-fibrin degradation products may not always reflect critical pathophysiologic mechanisms in DIC. This article aims to clarify the pathology of sepsis-associated DIC using assessment of comprehensive coagulation and fibrinolysis. Methods Plasma samples were obtained from 57 patients with sepsis-associated DIC at the time of initial diagnosis. Hemostasis parameters were quantified by clot-fibrinolysis waveform analysis (CFWA) and thrombin/plasmin generation assays (T/P-GA). The results were expressed as ratios relative to normal plasma. Results CFWA demonstrated that the maximum coagulation velocity (|min1|) ratio modestly increased to median 1.40 (min − max: 0.10 − 2.60) but the maximum fibrinolytic velocity (|FL-min1|) ratio decreased to 0.61 (0 − 1.19). T/P-GA indicated that the peak thrombin (Th-Peak) ratio moderately decreased to 0.71 (0.22 − 1.20), whereas the peak plasmin (Plm-Peak) ratio substantially decreased to 0.35 (0.02 − 1.43). Statistical comparisons identified a correlation between |min1| and Th-Peak ratios (ρ = 0.55, p < 0.001), together with a strong correlation between |FL-min1| and Plm-Peak ratios (ρ = 0.71, p < 0.001), suggesting that CFWA reflected the balance between thrombin and plasmin generation. With |min1| and |FL-min1| ratios, DIC was classified as follows: coagulation-predominant, coagulation/fibrinolysis-balanced, fibrinolysis-predominant, and consumption-impaired coagulation. The majority of patients in our cohort (80.7%) were coagulation-predominant. Conclusion A pathological clarification of sepsis-associated DIC based on the assessment of coagulation and fibrinolysis dynamics may be useful for the hemostatic monitoring and management of optimal treatment in these individuals.

scholarly journals Clinical Features of Disseminated Intravascular Coagulation According to the French-American-British Classification in Patients With Acute Leukemia and Thrombomodulin Alfa Treatment—A Cohort Study Using a Postmarketing Surveillance Database

2021 ◽  
Vol 27 ◽  
pp. 107602962110540
Author(s):  
Seki Yoshinobu ◽  
Goichi Honda ◽  
Noriaki Kawano ◽  
Toshimasa Uchiyama ◽  
Kazuo Kawasugi ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Clinical Features ◽  
Disseminated Intravascular Coagulation ◽  
Lymphoblastic Leukemia ◽  
Postmarketing Surveillance ◽  
Intravascular Coagulation ◽  
Plasmin Inhibitor ◽  
Surveillance Database ◽  
French American British

The aims of this study were to analyze the clinical features of a large number of cases with disseminated intravascular coagulation (DIC) associated with acute leukemia and to assess the safety and efficacy of thrombomodulin alfa (TM-α) using the French-American-British (FAB) classification of hematological malignancies. We retrospectively examined 644 patients with acute leukemia in postmarketing surveillance for TM-α. M3, M2, M4, M1, and M5 subtypes of acute myeloid leukemia (AML) and L2 and L1 subtypes of acute lymphoblastic leukemia (ALL) have been found more frequently among patients with DIC. Bleeding symptoms at baseline were more frequent in M3 and M7 subtypes. Fibrinogen concentrations were lower, and plasmin-plasmin inhibitor complex values were higher in M3 and Philadelphia-positive (Ph+) ALL. Overall DIC resolution rate was 60.2%, higher in L1 and Ph+ ALL, lower in M1, and generally higher in ALL than in AML. Overall survival rate was generally high, at 79.8%, with higher rates in L3, Ph+ ALL, and M3. Regardless of FAB subgroup, TM-α showed improved bleeding symptoms and DIC scores in clinical practice for DIC patients with acute leukemia.

Outcome of Disseminated Intravascular Coagulation in Relation to the Score when Treatment was Begun

1995 ◽  
Vol 74 (03) ◽  
pp. 848-852 ◽  
Author(s):  
Hideo Wada ◽  
Yoshihiro Wakita ◽  
Tutomu Nakase ◽  
Minori Shimura ◽  
Katsuyo Hiyoyama ◽  
...  
Keyword(s):  
Disseminated Intravascular Coagulation ◽  
Plasma Levels ◽  
Early Stage ◽  
Coagulation Factors ◽  
Underlying Disease ◽  
Blood Coagulation Factors ◽  
Intravascular Coagulation ◽  
The Relationship ◽  
Plasmin Inhibitor ◽  
D Dimer

SummaryWe examined 395 patients with disseminated intravascular coagulation (DIC) divided into two groups: non-leukemic and leukemic. In 58% of the patients as a whole, treatment of DIC resulted in complete or partial remission, while exacerbation and death occurred in 31%. The efficacy of DIC treatment in the non-leukemic group was less than that in the leukemic group, indicating that the outcome of DIC depended, in part, on the underlying disease. We examined hemostatic indicators in relation to DIC score: prothrombin time (PT) ratio, FDP, platelet count, and fibrinogen levels were found to be important indicators for the diagnosis of DIC, but not for Pre-DIC. Plasma levels of fibrin-D-dimer, thrombin-antithrombin complex (TAT), and plasmin- plasmin inhibitor complex (PPIC) were significantly increased in pre-DIC. The efficacy of treatment in relation to the DIC score when the treatment was begun showed that greater efficacy was achieved in pre-DIC than in DIC patients. The outcome was poorer with increasing DIC score, suggesting that early diagnosis and early treatment are important. On examining the relationship between outcome and hemostatic indicators, we found that the PT ratio and the levels of antithrombin, plasminogen, PPIC, the PPIC/TAT ratio, and thrombomodulin were related to outcome, suggesting that very high consumption of blood coagulation factors, liver dysfunction, hypofibrinolysis, or organ failure caused a poor outcome. Although the outcome in DIC patients may not depend substantially on plasma levels of TAT and fibrin-D- dimer, we can use these indicators to treat DIC patients at an early stage.

scholarly journals ШОК ТА ДВЗ-СИНДРОМ ЯК ПАТОГЕНЕТИЧНА ВІСЬ ЗА БАБЕЗІОЗУ СОБАК

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 70-74
Author(s):  
O.A. Dubova
Keyword(s):  
Cause Of Death ◽  
Disseminated Intravascular Coagulation ◽  
Degradation Products ◽  
Clinical Signs ◽  
Vicious Circle ◽  
Consumption Coagulopathy ◽  
Soluble Fibrin ◽  
Intravascular Coagulation ◽  
Determining Factors ◽  
Morphological Picture

The article is devoted to research of complication of dogs babesiosis and the elucidation of their pathogenetic mechanisms. Babesiosis of dogs is extremely common in Polissya region of Ukraine, as created ideal conditions for enzootic focus. Agents have considerable virulence, so the disease is often accompanied by extremely severe complications. In our researches it is established that the main pathogenetic axis represent the shock and syndrome of disseminated intravascular coagulation of blood. These two processes are the secondary and create a "vicious circle" mutually causing and utilise each other. They are the determining factors of the death of the body.We studied clinical signs, laboratory indicators and pathological and morphological picture of complication. Clearly the stage of hypercoagulability with the defeat of the shock bodies, of consumption coagulopathy with bleeding on the background of thrombosis, fibrinolysis with ongoing bleeding and further irreversible terminal stage of shock. The main involving laboratory criteria are the concentration of degradation products of fibrinogen/fibrin and the presence of soluble fibrin-monomer complexes defined in the ethanol test, and hematocrit. Other indicators and signs allow you to identify the stage of complications.Thus, when dogs babesiosis main complications are disseminated intravascular coagulation and shock that affect the vital organs and systems and they are the main determining cause of death of animals.

Plasma Thrombin Assay using a Chromogenic Substrate in Disseminated Intravascular Coagulation due to Snake Bite Envenomation

1979 ◽  
Vol 41 (03) ◽  
pp. 544-552 ◽  
Author(s):  
R P Herrmann ◽  
P E Bailey
Keyword(s):  
Disseminated Intravascular Coagulation ◽  
Degradation Products ◽  
Snake Bite ◽  
Chromogenic Substrate ◽  
Coagulation Parameters ◽  
Fibrinogen Degradation Products ◽  
Intravascular Coagulation

SummaryUsing the chromogenic substrate, Tos-Gly-Pro-Arg-pNA-HCL (Chromozym TH, Boehringer Mannheim) plasma thrombin was estimated in six cases of envenomation by Australian elapid snakes. All patients manifested findings chracteristic of defibrination due to envenomation by these snakes. Fibrin-fibrinogen degradation products were grossly elevated, as was plasma thrombin in all cases.Following treatment with antivenene, all abnormal coagulation parameters returned rapidly towards normal by 24 hours and plasma thrombin disappeared.

Platelet Function in Experimentally Induced Pancreatitis in the Dog

1986 ◽  
Vol 55 (02) ◽  
pp. 197-200 ◽  
Author(s):  
R M Jacobs ◽  
R J Murtaugh ◽  
R H Fertel
Keyword(s):  
Platelet Function ◽  
Disseminated Intravascular Coagulation ◽  
Degradation Products ◽  
Plasma Concentrations ◽  
Adenosine Diphosphate ◽  
Intravascular Coagulation ◽  
Fibrin Degradation Products ◽  
Functional Defect ◽  
And Function ◽  
Experimentally Induced

SummaryEvidence suggests that changes in prostaglandins and disseminated intravascular coagulation accompany pancreatitis. Both may induce changes in platelet function. We wished to determine if experimentally induced pancreatitis in the dog was associated with altered platelet number and function, and whether there were concomitant changes in prostaglandins. Evidence for disseminated intravascular coagulation in the dogs with pancreatitis were red blood cell fragmentation, increased platelet turnover indicated by macro-platelets and the transient presence of fibrin degradation products in urine. There were no significant changes in platelet count. The platelets from dogs with pancreatitis showed a functional defect characterized by significantly decreased aggregation in response to adenosine diphosphate, arachidonic acid, and collagen. Release of adenosine triphosphate from platelets was reduced in collagen-stimulated aggregation. There were no changes in the plasma concentrations of thromboxane B2, 6-Keto-PGF1a, and PGE2. This defect may have been due to the generation of fibrin degradation products and platelet “exhaustion”.

Changes in Plasma Levels of Prothrombin Fragment F1+2 in Cases of Disseminated Intravascular Coagulation

1993 ◽  
Vol 89 (1) ◽  
pp. 22-25 ◽  
Author(s):  
Hidesaku Asakura ◽  
Yoshimune Shiratori ◽  
Hiroshi Jokaji ◽  
Masanori Saito ◽  
Chika Uotani ◽  
...  
Keyword(s):  
Disseminated Intravascular Coagulation ◽  
Plasma Levels ◽  
Prothrombin Fragment ◽  
Intravascular Coagulation
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