Selection of potential probiotics with cholesterol-lowering properties for probiotic yoghurt production

2021 ◽  
pp. 108201322110122
Author(s):  
Saranya Wongrattanapipat ◽  
Anchukorn Chiracharoenchitta ◽  
Budsarin Choowongwitthaya ◽  
Prapatson Komsathorn ◽  
Orawan La-ongkham ◽  
...  
Keyword(s):  
Antibiotic Resistance Genes ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Bile Salt Hydrolase ◽  
Cholesterol Lowering ◽  
Sensory Evolution ◽  
Probiotic Strains ◽  
Total Bacteria ◽  
Selection Of ◽  
Bsh Activity

From 61 lactic acid bacteria (LAB) isolates, three had good cholesterol-lowering properties, with Limosilactobacillus fermentum KUB-D18 having the highest cholesterol assimilation (68.75%) (51 µg/109 CFU). In addition, Lactiplantibacillus pentosus HM04-25 and L. pentosus HM04-3 had the two highest levels of bile salt hydrolase (BSH) activity (22.60 and 21.45 U/mL, respectively). These three strains could resist four antibiotics (aztreonam, vancomycin, teicoplanin, and nalidixic). However, fortunately, they contained no mobile antibiotic resistance genes. To evaluate the influence of probiotic strains in yoghurt production, L. fermentum KUB-D18, L. pentosus HM04-25, or L. pentosus HM04-3 were simultaneously cultured with commercial yoghurt starter (YF-L812) and incubated at 43 °C for 6 h. During yoghurt fermentation, the total bacteria in the yoghurt tended to increase from 7.39 to 8.90 log CFU/mL. The growth rates of two probiotic strains ( L. pentosus HM04-25 and L. pentosus HM04-3) were stable at 6.06 to 6.62 log CFU/mL. Only the rate for L. fermentum KUB-D18 increased (to 7.5 log CFU/mL). These three probiotics did not affect the physical characteristics of yoghurt. The total soluble solids, pH, and titratable acidity values of the probiotic yoghurts were similar to the control yoghurt at 30[Formula: see text]Brix, 4.91, and 0.90%, respectively. The firmness values of the probiotic yoghurts and the control were not significantly different (p > 0.05). Differentiation of the appearance of color, odor, flavor, and texture between the control yoghurt and the probiotic yoghurts was investigated using 56 volunteers and no significant differences were identified. Additionally, sensory evolution revealed that the acceptability of the probiotic yoghurts was higher than for the control (p ≤ 0.05). Therefore, the three probiotic strains with cholesterol-lowering properties had potential in future yoghurt production.

scholarly journals In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 674
Author(s):  
Jimmy G. Hernández-Gómez ◽  
Argelia López-Bonilla ◽  
Gabriela Trejo-Tapia ◽  
Sandra V. Ávila-Reyes ◽  
Antonio R. Jiménez-Aparicio ◽  
...  
Keyword(s):  
Bile Salt ◽  
High Performance ◽  
Probiotic Strain ◽  
Saccharomyces Boulardii ◽  
Bile Salt Hydrolase ◽  
Sodium Glycocholate ◽  
Probiotic Yeast ◽  
Probiotic Strains ◽  
Bsh Activity

Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.

scholarly journals Physicochemical Quality, Antioxidant Capacity and Nutritional Value in Tuberous Roots of Some Wild Dahlia Species

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Esteban A. RIVERA-ESPEJEL ◽  
Oscar CRUZ-ALVAREZ ◽  
José M. MEJÍA-MUÑOZ ◽  
María R. GARCÍA-MATEOS ◽  
María T.B. COLINAS-LEÓN ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Nutritional Value ◽  
Block Design ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Digital Object Identifier ◽  
Physicochemical Quality ◽  
Tuberous Roots ◽  
Randomized Complete Block Design ◽  
Selection Of

The aim of this research was to evaluate the physicochemical quality, antioxidant capacity and nutritional value in tuberous roots of some wild dahlia species. The experiment was carried out in the Department of Plant Science of the Autonomous University Chapingo, Mexico. Plants were established in a randomized complete block design with five replications. The total soluble solids (TSS), titratable acidity (TA), vitamin C (VC), total phenols (TP), antioxidant capacity (AC), inulin and its proximate composition were evaluated. Among the materials analyzed, the most outstanding wild species were Dahlia campanulata, D. coccinea and D. brevis, where D. campanulata stood out for its concentration of VC (0.05 mg 100 g-1), AC (1.88 mg VCEAC g-1), inulin, DM and TC (72.25, 24.38 and 88.37%, respectively), however, the inulin content was similar to D. coccinea (66.17%), which was also outstanding with respect to the content of TP (1.74 mg GA g-1). Likewise, D. brevis presented the highest values of RFi (5.49%) and A (78.42%). According to our results, the tuberous roots of wild dahlia species can be used as food, as well as being a source of selection of traits of nutraceutic interest for genetic improvement.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********  

scholarly journals Evaluation of viability of probiotic bacteria in mango (Mangifera indica L. Cv. “Tommy Atkins”) beverage

DYNA ◽  
2018 ◽  
Vol 85 (207) ◽  
pp. 84-92 ◽  
Author(s):  
Elizabeth Cristina Acevedo-Martínez ◽  
Carolina Gutiérrez Cortés ◽  
Maribel García Mahecha ◽  
Consuelo Díaz Moreno
Keyword(s):  
Storage Temperature ◽  
Mangifera Indica ◽  
Titratable Acidity ◽  
Probiotic Bacteria ◽  
Functional Beverages ◽  
Probiotic Strains ◽  
Three Stages ◽  
Technological Challenge ◽  
And Storage ◽  
Selection Of

The interest about probiotic bacteria supplementation in fruit beverages has been increased recently in order to develop functional beverages without lactose. That is a technological challenge since pH and storage temperature can affect their viability. The aim of this study was to evaluate the viability of three commercial probiotic strains (L. paracasei, L. casei and L. rhamnosus) in mango beverage stored at 4°C. This research was developed in three stages: the assessment of the acid strain tolerance, the selection of a prebiotic as cellular protectant against environmental stress, the evaluation of physiochemical and sensory stability during storage. L. casei was chosen as the most stable strain with FOS at 5% as a prebiotic substance that stimulated its growth. At the final stage, it was found that L. casei produced significant changes in pH and titratable acidity in mango beverages after one week at 4°C, which was also detected by consumers with good sensory acceptance

scholarly journals Stratified mass selection of promising Spondias mombin clones in a commercial crop

2011 ◽  
Vol 11 (2) ◽  
pp. 141-148 ◽  
Author(s):  
José Airton Rodrigues Nunes ◽  
Francisco Ferreira Santana ◽  
Regina Lúcia Ferreira Gomes ◽  
Ângela Celis de Almeida Lopes ◽  
Maria Marlúcia Gomes Pereira ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Pulp Yield ◽  
Mass Selection ◽  
Spondias Mombin ◽  
Growing Seasons ◽  
Commercial Crop ◽  
Selection Of ◽  
Private Farm

This study aimed to estimate genetic parameters and identify promising clones of Spondias mombin in ungrafted commercial crop by stratified mass selection, based on the average of two harvests, using mixed models. The base population growing on a private farm in Teresina/PI comprised 82 plants, which were evaluated in the growing seasons 2008 and 2009. Total titratable acidity (TTA), total soluble solids (Brix), Brix/TTA, fruit weight (FW), seed weight (SW), pulp yield (PuY), and processing yield (PrY) were measured. Significant genetic variation among plants was detected for all traits. The coefficients of repeatability were 0.97 (TTA), 0.39 (Brix), 0.86 (Brix/TTA), 0.84 (FW), 0.82 (SW), 0.35 (PuY), and 0.39 (PrY). The chemical were generally not correlated with the physical fruit traits. Ranking by predicted phenotypic values associated with PrY and Brix/TTA identified nine promising plants (PrY>6.9% and Brix/TTA>10).

scholarly journals Cholesterol-Lowering Potentials of Lactic Acid Bacteria Based on Bile-Salt Hydrolase Activity and Effect of Potent Strains on Cholesterol MetabolismIn VitroandIn Vivo

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Cheng-Chih Tsai ◽  
Pei-Pei Lin ◽  
You-Miin Hsieh ◽  
Zi-yi Zhang ◽  
Hui-Ching Wu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bile Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Hepg2 Cells ◽  
Bile Salt Hydrolase ◽  
Apo B ◽  
Probiotic Strains ◽  
Bile Salt Hydrolase Activity ◽  
Bsh Activity

This study collected different probiotic isolates from animal and plant sources to evaluate the bile-salt hydrolase activity of probioticsin vitro. The deconjugation potential of bile acid was determined using high-performance liquid chromatography. HepG2 cells were cultured with probiotic strains with high BSH activity. The triglyceride (TG) and apolipoprotein B (apo B) secretion by HepG2 cells were evaluated. Our results show that the BSH activity and bile-acid deconjugation abilities ofPediococcus acidilacticiNBHK002,Bifidobacterium adolescentisNBHK006,Lactobacillus rhamnosusNBHK007, andLactobacillus acidophilusNBHK008 were higher than those of the other probiotic strains. The cholesterol concentration in cholesterol micelles was reduced within 24 h. NBHK007 reduced the TG secretion by 100% after 48 h of incubation. NBHK002, NBHK006, and NBHK007 could reduce apo B secretion by 33%, 38%, and 39%, respectively, after 24 h of incubation. The product PROBIO S-23 produced a greater decrease in the total concentration of cholesterol, low-density lipoprotein, TG, and thiobarbituric acid reactive substance in the serum or livers of hamsters with hypercholesterolemia compared with that of hamsters fed with a high-fat and high-cholesterol diet. These results show that the three probiotic strains of lactic acid bacteria are better candidates for reducing the risk of cardiovascular disease.

scholarly journals Identification of Bile Salt Hydrolase and Bile Salt Resistance in a Probiotic Bacterium Lactobacillus gasseri JCM1131T

2021 ◽  
Vol 9 (5) ◽  
pp. 1011
Author(s):  
Hiroyuki Kusada ◽  
Kana Morinaga ◽  
Hideyuki Tamaki
Keyword(s):  
Bile Salt ◽  
Salt Resistance ◽  
Probiotic Bacterium ◽  
Lactobacillus Species ◽  
Bile Salt Hydrolase ◽  
Lactobacillus Gasseri ◽  
Enzymatic Function ◽  
Related Enzyme ◽  
Selection Of ◽  
Bsh Activity

Lactobacillus gasseri is one of the most likely probiotic candidates among many Lactobacillus species. Although bile salt resistance has been defined as an important criterion for selection of probiotic candidates since it allows probiotic bacteria to survive in the gut, both its capability and its related enzyme, bile salt hydrolase (BSH), in L. gasseri is still largely unknown. Here, we report that the well-known probiotic bacterium L. gasseri JCM1131T possesses BSH activity and bile salt resistance capability. Indeed, this strain apparently showed BSH activity on the plate assay and highly tolerated the primary bile salts and even taurine-conjugated secondary bile salt. We further isolated a putative BSH enzyme (LagBSH) from strain JCM1131T and characterized the enzymatic function. The purified LagBSH protein exhibited quite high deconjugation activity for taurocholic acid and taurochenodeoxycholic acid. The lagBSH gene was constitutively expressed in strain JCM1131T, suggesting that LagBSH likely contributes to bile salt resistance of the strain and may be associated with survival capability of strain JCM1131T within the human intestine by bile detoxification. Thus, this study first demonstrated the bile salt resistance and its responsible enzyme (BSH) activity in strain JCM1131T, which further supports the importance of the typical lactic acid bacterium as probiotics.

scholarly journals Field Performance of 20 `Pinot noir' Clones in the Willamette Valley of Oregon

2006 ◽  
Vol 16 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Steven P. Castagnoli ◽  
M. Carmo Vasconcelos
Keyword(s):  
Clonal Selection ◽  
Field Performance ◽  
Titratable Acidity ◽  
Pinot Noir ◽  
Soluble Solids ◽  
Willamette Valley ◽  
Climate Conditions ◽  
Solids Concentration ◽  
Anthocyanin Concentration ◽  
Selection Of

Clonal selection of European winegrapes (Vitis vinifera) allows the exploitation of an important source of genetic diversity. In the 1980s, many `Pinot noir' clones, previously unavailable in the U.S., were imported from France. To provide information on their performance under Oregon soil and climate conditions, 20 `Pinot noir' clones were established in a replicated trial in Alpine, Ore., planted in 1989. In this study, yield components, pruning weight, and juice composition of the 20 clones were measured for the 1995 through 1999 seasons. Skin anthocyanin concentration was measured for the 1996 through 1999 seasons. Clones included in the trial were Colmar (COL) 538, Dijon (DJN) 10/18, DJN 113, DJN 114, DJN 115, DJN 375, DJN 60, Espiguette (ESP) 236, ESP 374, Foundation Plant Services (FPS) 2A, FPS 4, FPS 10, FPS 16, FPS 17, FPS 22, FPS 23, FPS 29, FPS 31, FPS 32, and FPS 33. For all responses except juice pH and skin anthocyanin concentration, there were significant clone by year interactions. COL 538 had the highest 5-year mean yield (2.93 kg/vine); FPS 29 had the lowest (1.21 kg/vine). DJN 10/18, FPS 4, FPS 22, and FPS 31 were among the five highest-yielding clones. Other low-yielding clones included DJN 115, ESP 374, FPS 17, and FPS 23. Pruning weights were generally correlated with yields. COL 538 had the highest 5-year mean pruning weight (0.81 kg/vine) and FPS 17 had the lowest (0.48 kg/vine). Other clones with relatively high pruning weights were FPS 2A, FPS 4, and FPS 22. Other clones with low pruning weights were FPS 23 and FPS 29. FPS 22 and FPS 17 had the highest (1.13 g/berry) and lowest (0.93 g/berry) 5-year mean berry weights, respectively. Clones with 5-year mean cluster weights >100 g included DJN 10/18, ESP 236, and FPS 31. Those with cluster weights <80 g were DJN 115, FPS 17, and FPS 29. FPS 2A had the highest 5-year mean juice soluble solids concentration (SSC) at harvest (23.8%). FPS 10, FPS 29, DJN 113, and DJN 115 also had relatively high SSC. DJN 60 had the lowest 5-year mean SSC at harvest (22.0%). FPS 22, FPS 33, COL 538, and ESP 374 also had relatively low 5-year mean SSC at harvest. DJN 115 had the highest 5-year mean juice pH (3.15). DJN 113, FPS 29, and FPS 10 also had relatively high juice pH. FPS 22 had the lowest 5-year mean juice pH at harvest (2.97), and DJN 10/18, FPS 2A, and FPS 17 also had relatively low pH. Clones with higher SSC and pH generally had lower titratable acidity. FPS 2A had both high SSC and high titratable acidity. FPS 23 and FPS 17 had the highest skin anthocyanin concentration (2.10 and 2.07 mg·g-1 berry, respectively). The range of skin anthocyanin concentration among the other clones was relatively narrow (1.17-1.47 mg·g-1 berry). FPS 2A, FPS 4, and FPS 10 generally had above mean SSC and yield. FPS 29, DJN 113, DJN 114, and DJN 115 consistently had above mean SSC but below mean yield.

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