High-Throughput Screening Assay for Sphingosine Kinase Inhibitors in Whole Blood Using RapidFire® Mass Spectrometry
2011 ◽
Vol 16
(2)
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pp. 272-277
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Keyword(s):
To facilitate discovery of compounds modulating sphingosine-1-phosphate (S1P) signaling, the authors used high-throughput mass spectrometry technology to measure S1P formation in human whole blood. Since blood contains endogenous sphingosine (SPH) and S1P, mass spectrometry was chosen to detect the conversion of an exogenously added 17-carbon-long variant of sphingosine, C17SPH, into C17S1P. The authors developed procedures to achieve homogeneous mixing of whole blood in 384-well plates and for a method requiring minimal manipulations to extract S1P from blood in 96- and 384-well plates prior to analyses using the RapidFire® mass spectrometry system.
2016 ◽
Vol 6
(6)
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pp. 557-563
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2003 ◽
Vol 8
(2)
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pp. 198-204
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2008 ◽
Vol 627
(1)
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pp. 105-111
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2000 ◽
Vol 5
(6)
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pp. 463-470
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2020 ◽
Vol 25
(9)
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pp. 1064-1071
Keyword(s):
2012 ◽
Vol 18
(3)
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pp. 298-308
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