Comparison of the effect of three intramuscular sedation protocols on packed cell volume and total protein in cats

2021 ◽  
pp. 1098612X2110647
Author(s):  
Alicia M Skelding ◽  
Alexander Valverde
Keyword(s):  
Clinical Study ◽  
Blood Sample ◽  
Cell Volume ◽  
Total Protein ◽  
Packed Cell Volume ◽  
Venous Blood ◽  
Blood Sampling ◽  
Venous Blood Sample ◽  
To Receive ◽  
Venous Blood Sampling

Objectives The aim of this study was to evaluate the change in packed cell volume (PCV) and total protein following intramuscular preanesthetic sedation with one of three drug combinations in cats. Methods Thirty client-owned cats were enrolled in this prospective, randomized, blinded, clinical study. A venous blood sample was obtained prior to administration of any sedation and PCV, total protein, electrolytes (Na+, K+, Cl–, iCa2+), glucose and lactate were measured. Cats were randomly assigned to receive one of three intramuscular sedation protocols (n = 10 cats/protocol): methadone 0.2 mg/kg + acepromazine 0.02 mg/kg (MA), methadone 0.2 mg/kg + dexmedetomidine 5 µg/kg (MD) or methadone 0.2 mg/kg + midazolam 0.2 mg/kg + alfaxalone 2 mg/kg (MMA). Twenty-five minutes later, cats were assessed for level of sedation followed by another venous blood sampling to evaluate the same variables as above. Results There were no significant differences in demographics (age, weight, sex) between groups. Level of sedation was significantly higher in MMA cats. Within groups, after premedication, PCV and hemoglobin significantly decreased in all groups, total protein significantly decreased in the MA and MMA groups and glucose significantly increased in the MD group. For electrolytes, statistical changes were not clinically relevant; Cl– mean difference was significantly different between MA and MD; in the MD group Na+ and Cl– significantly decreased and in the MMA group Cl– significantly increased. Conclusions and relevance All three sedation protocols caused significant decreases in PCV and hemoglobin in healthy cats.

scholarly journals Thyroid hormones, blood plasma metabolites and haematological parameters in relationship to milk yield in dairy cows

1983 ◽  
Vol 36 (1) ◽  
pp. 93-104 ◽  
Author(s):  
J. W. Blum ◽  
P. Kunz ◽  
H. Leuenberger ◽  
K. Gautschi ◽  
M. Keller
Keyword(s):  
Thyroid Hormones ◽  
Cell Volume ◽  
Milk Production ◽  
Milk Yield ◽  
Total Protein ◽  
Packed Cell Volume ◽  
Venous Blood ◽  
Blood Parameters ◽  
Plasma Metabolites ◽  
Dry Period

ABSTRACTTo study their relationship to milk yield, the concentrations, in jugular venous blood, of thyroxine iodine (T4I), thyroxine (T4), 3,5,3'-tri-iodothyronine (T3), glucose, non-esterified fatty acids (NEFA), triglycerides, phospholipids, cholesterol, total protein, albumin, urea, haemoglobin and packed cell volume (PCV) have been measured in 36 cows (Simmental, Swiss Brown, Holstein and Simmental × Holstein) of different ages during a full lactation, pregnancy, dry period, parturition and 150 days of the ensuing lactation. Thyroid hormones and triglycerides were negatively, and total protein, globulin, cholesterol and phospholipids were positively, correlated with uncorrected or corrected milk yield during several periods of lactation, whereas glucose, NEFA, albumin, urea, haemoglobin and packed cell volume were not correlated with milk yield. The 10 animals with the highest milk yield (18·9 to 23·5 kg/day) exhibited significantly lower values of T4I, T4, T3 and glucose, significantly higher levels of total protein and globulin and tended to have higher levels of NEFA than the 10 cows with the lowest milk yield (10·9 to 14·3 kg/day) throughout or during certain periods of lactation, whereas concentrations of triglycerides, phospholipids, cholesterol, albumin, haemoglobin and PCV did not differ. Changes in T4I, T4, T3, glucose and total protein during lactation were also influenced by age, presumably associated with an increase in milk production with age. T3 was consistently lowest and cholesterol and phospholipids, during later stages of lactation, were highest in Holsteins, which had the highest milk yields of all breeds. Changes of blood parameters were mainly caused by shifts in energy and protein metabolism in association with level of milk production

scholarly journals An Anti-Nucleocapsid Antigen Sars-Cov-2 Total Antibody Assay Finds Comparable Results in Edta-Anticoagulated Whole Blood Obtained from Capillary and Venous Blood Sampling

Data ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 105
Author(s):  
Martin Risch ◽  
Marc Kovac ◽  
Corina Risch ◽  
Dorothea Hillmann ◽  
Michael Ritzler ◽  
...  
Keyword(s):  
Whole Blood ◽  
Venous Blood ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Attractive Alternative ◽  
Antibody Assay ◽  
Antibody Assays ◽  
Antibody Titers ◽  
Antibody Levels ◽  
Venous Blood Sampling

Although SARS-CoV-2 antibody assays have been found to provide valid results in EDTA-anticoagulated whole blood, so far, they have not demonstrated that antibody levels in whole blood originating from capillary blood samples are comparable to antibody levels measured in blood from a venous origin. Here, blood is drawn simultaneously by capillary and venous blood sampling. Antibody titers are determined by an assay employing electrochemiluminescence (ECLIA) and SARS-CoV-2 total immunoglobulins are detected with specificity directed against the nucleocapsid antigen. Six individuals with confirmed COVID-19 and six individuals without COVID-19 are analyzed. Antibody titers in capillary venous whole blood did not show significant differences, and when corrected for hematocrit, they did not differ from the results obtained from serum. In conclusion, capillary sampled EDTA-anticoagulated whole blood seems to be an attractive alternative matrix for the evaluation of SARS-CoV-2 antibodies when employing ECLIA for detecting total antibodies directed against nucleocapsid antibodies.

scholarly journals Improving the quality of venous blood sampling procedure (phlebotomy): avoiding tourniquet use

2020 ◽  
Author(s):  
Francisco Freitas ◽  
Mónica Alves
Keyword(s):  
Venous Blood ◽  
Real Life ◽  
Sampling Procedure ◽  
T Test ◽  
Blood Sampling ◽  
Age Group ◽  
The Individual ◽  
Sampling Procedures ◽  
Without Tourniquet ◽  
Venous Blood Sampling

AbstractBackgroundGuidelines for venous blood sampling procedure (phlebotomy) discourage tourniquet use whenever possible. Here, we aimed to assess the Biomedical Scientists capability of not using the tourniquet in phlebotomy, which we hypothesized to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site.Materials and MethodsWe selected and assigned two (BMS) with the same age (41 years) and experience (20 years) to record ten phlebotomy days, the first with prioritized and the latter with non-prioritized patients. In a simple record form, each acquired daily data for the number of attended patients, age and gender, the frequency of non-tourniquet usage and the punctured vein. To test our work hypothesis we used the two-tailed single sample t-test (p < 0.05). Differences between age-group means and non-tourniquet use means by each BMS were tested by two-tailed t-test for independent means (p < 0.05).ResultsIn 10 phlebotomy days 683 patients were attended, with males representing 43,2% of the population. We found no statistically difference between age-group means. The combined capability of non-tourniquet use was 50,5%, which did not differ from our null hypothesis, but the individual group-means were statistically different, being 33% and 66.9% in the prioritized vs non-prioritized group. The medial cubital vein was the most prone to be punctured (77,7%).ConclusionsWe have shown that performing phlebotomies without tourniquet use is possible and desirable in at least half of the attended patients, though being more limited in specific group populations. Our results provide room for quality improvement in the laboratory pre-analytical phase.Key points summaryWe assessed the capability of Biomedical Scientists not using the tourniquet in real life blood sampling procedures for diagnostic purposes.Blood was collected from at least half of the attended patients without tourniquet use.Biomedical Scientists were able to prioritize the antecubital veins without tourniquet application (medial cubital vein the most prone to be punctured - 78% of attempts).

scholarly journals PENGARUH INFEKSI CACING Ascaridia galli TERHADAP GAMBARAN DARAH DAN ELEKTROLIT AYAM KAMPUNG (Gallus domesticus)

2015 ◽  
Vol 9 (1) ◽  
Author(s):  
Joko Prastowo ◽  
Bambang Ariyadi
Keyword(s):  
Cell Volume ◽  
Total Protein ◽  
Packed Cell Volume ◽  
Gallus Domesticus ◽  
Ascaridia Galli

Penelitian ini bertujuan mengetahui pengaruh infeksi telur cacing Ascaridia galli (A. galli) terhadap elektrolit dan gambaran darah ayam kampung (Gallus domesticus). Kelompok perlakuan dilakukan infeksi telur berembrio cacing A. galli sebanyak 500 telur cacing/ekor ayam. Sampel berupa feses ayam untuk pemeriksaan parasitologi dan darah untuk pemeriksaan elektrolit dan pemeriksaan darah rutin. Infeksi cacing A.galli menyebabkan penurunan kadar kalium serum pada hari ke-21 dan 28 setelah infeksi (P<0,05), kenaikan kadar magnesium serum pada hari ke-21 dan 28 setelah infeksi (P<0,05) dan tidak memberikan pengaruh terhadap kadar natrium serum setelah infeksi. Hasil penelitian ini menyebabkan penurunan terhadap jumlah eritrosit pada hari ke-7 dan 14 setelah infeksi (P<0,05), penurunan terhadap nilai packed cell volume (PCV) pada hari ke-14 setelah infeksi (P<0,05), kenaikan nilai total protein plasma pada hari ke-7 setelah infeksi (P<0,05), kenaikan nilai absolut sel eosinofil pada hari ke-14 setelah infeksi (P<0,05), tidak memberikan pengaruh terhadap kadar hemoglobin, jumlah leukosit, nilai absolut sel heterofil, limfosit, dan monosit. Pada hari ke-28 setelah infeksi, rerata cacing yang hidup yaitu 13 ekor cacing. Dari hasil penelitian disimpulkan bahwa, infeksi 500 telur cacing berembrio A. galli menyebabkan penurunan kadar kalium, kenaikan kadar magnesium, penurunan terhadap jumlah eritrosit, penurunan terhadap nilai PCV, kenaikan nilai total protein plasma, kenaikan nilai absolut sel eosinofil, dan tidak memberikan pengaruh terhadap natrium, kadar hemoglobin, jumlah leukosit, nilai absolut sel heterofil, nilai absolut limfosit, dan nilai absolut monosit.

scholarly journals How to Perform Umbilical Cord Arterial and Venous Blood Sampling in Neonatal Foals

2019 ◽  
Vol 73 ◽  
pp. 24-29 ◽  
Author(s):  
Sunita S. Jeawon ◽  
Lisa M. Katz ◽  
Noreen P. Galvin ◽  
Stephen D. Cahalan ◽  
Vivienne E. Duggan
Keyword(s):  
Umbilical Cord ◽  
Venous Blood ◽  
Blood Sampling ◽  
Venous Blood Sampling
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