Detection of Epstein-Barr virus Genome in Squamous Cell Carcinomas of the Larynx

1995 ◽  
Vol 10 (4) ◽  
pp. 211-215 ◽  
Author(s):  
H. Kiaris ◽  
M. Ergazaki ◽  
J. Segas ◽  
D.A. Spandidos
Keyword(s):  
Squamous Cell ◽  
Normal Tissue ◽  
Epstein Barr Virus ◽  
Tumor Tissue ◽  
Virus Genome ◽  
Squamous Cell Carcinomas ◽  
Adjacent Normal Tissue ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

Epstein-Barr virus (EBV) is a B-lymphotropic virus with a tumorigenic potential. EBV infection has been recognized as the main cause of nasopharyngeal carcinoma and Burkitt's lymphoma. The aim of our study was to determine the incidence of EBV in squamous cell carcinomas of the larynx. We employed for our analysis a sensitive polymerase chain reaction (PCR) assay, followed by restriction fragment length polymorphism (RFLP) for further confirmation of the specificity of the PCR-amplification reaction. Our analysis revealed that 9 of 27 (33%) specimens harbored the EBV genome in the tumor tissue while only 4 (15%) specimens from adjacent normal tissue exhibited evidence of EBV infection. Three were EBV positive for both normal and tumor tissue. No association has been found with disease stage, histological differentiation and nodes at pathology. The relatively high incidence of EBV in the tumor tissue (33%) of patients with laryngeal cancer, as compared to the low (15%) incidence of the virus genome detected in the adjacent normal tissue of the patients, indicates a probable role of EBV in the development of the disease.

Epstein-Barr virus genome-positive T lymphocytes in a boy with chronic active EBV infection associated with Kawasaki-like disease

Nature ◽  
1988 ◽  
Vol 333 (6172) ◽  
pp. 455-457 ◽  
Author(s):  
Hideaki Kikuta ◽  
Yuichi Taguchi ◽  
Kazuhiro Tomizawa ◽  
Kimikazu Kojima ◽  
Nobuaki Kawamura ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Epstein Barr Virus ◽  
Virus Genome ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

scholarly journals Do Epstein–Barr Virus Mutations and Natural Genome Sequence Variations Contribute to Disease?

Biomolecules ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 17
Author(s):  
Paul J. Farrell ◽  
Robert E. White
Keyword(s):  
Genome Sequence ◽  
Epstein Barr Virus ◽  
Sequence Variation ◽  
Virus Genome ◽  
Barr Virus ◽  
Ebv Infection ◽  
Sequence Variations ◽  
The World ◽  
Epstein Barr ◽  
Different Parts

Most of the world’s population is infected by the Epstein–Barr virus (EBV), but the incidence of the diseases associated with EBV infection differs greatly in different parts of the world. Many factors may determine those differences, but variation in the virus genome is likely to be a contributing factor for some of the diseases. Here, we describe the main forms of EBV genome sequence variation, and the mechanisms by which variations in the virus genome are likely to contribute to disease. EBV genome deletions or polymorphisms can also provide useful markers for monitoring disease. If some EBV strains prove to be more pathogenic than others, this suggests the possible value of immunising people against infection by those pathogenic strains.

Epstein-Barr virus infection is strictly associated with the metastatic spread of sinonasal squamous-cell carcinomas

Oral Oncology ◽  
2015 ◽  
Vol 51 (10) ◽  
pp. 929-934 ◽  
Author(s):  
Johannes Doescher ◽  
Guido Piontek ◽  
Markus Wirth ◽  
Marcus Bettstetter ◽  
Juergen Schlegel ◽  
...  
Keyword(s):  
Virus Infection ◽  
Squamous Cell ◽  
Epstein Barr Virus ◽  
Squamous Cell Carcinomas ◽  
Metastatic Spread ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Epstein Barr ◽  
Barr Virus Infection

scholarly journals A case of Epstein Barr virus-associated primary squamous cell carcinoma of stomach

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yuki Katsura ◽  
Takehiro Okabayashi ◽  
Kazuhiro Ozaki ◽  
Yuichi Shibuya ◽  
Jun Iwata
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Ebv Infection ◽  
Aortic Lymph Node ◽  
Epstein Barr

Abstract Background Primary squamous cell carcinoma (SCC) of stomach is extremely rare. The pathogenesis of SCC of stomach remains unclear. There is only one report that Epstein Barr virus (EBV) infection may be involved in the pathogenesis of SCC arising in the stomach ever before. Here, we report a case of Epstein Barr virus infection-associated primary SCC of stomach in a 70-year-old woman. She was presented to the referring hospital with hematemesis. Initial endoscopy revealed a bleeding gastric ulcer in the upper part of gastric corpus and the coagulation therapy was followed. After a 3-month follow-up, endoscopy revealed a submucosal tumor-like protrusion instead of an ulcer. Computed tomography revealed a mass in the upper part of stomach and swollen lymph nodes along with the lesser curvature and para-aortic lymph node. Biopsy could not confirm the definitive diagnosis. We performed total gastrectomy with para-aortic lymph node sampling. Histological analysis revealed squamous cell carcinoma with EBV infection with lymph node metastases. Tumor cells were positive for EBV-encoded small RNA (EBER) by in situ hybridization. The postoperative course was uneventful and the patient was discharged on day 11 after the operation. CapeOX was started as adjuvant chemotherapy, and the patient remains alive without recurrence 7 months after surgery. Conclusion This is the first case report of EBV infection-associated primary SCC of the stomach diagnosed by in situ hybridization of EBER. EBV infection may be related to the pathogenesis of primary SCC. Further evidence and studies are required to establish optimal strategy for this rare disease.

Detection of Epstein-Barr virus genome and latent infection gene expression in normal epithelia, epithelial dysplasia, and squamous cell carcinoma of the oral cavity

Tumor Biology ◽  
2015 ◽  
Vol 37 (3) ◽  
pp. 3389-3404 ◽  
Author(s):  
Kentaro Kikuchi ◽  
Yoshihiro Noguchi ◽  
Michelle Wendoline Garcia-Niño de Rivera ◽  
Miyako Hoshino ◽  
Hideaki Sakashita ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epstein Barr Virus ◽  
Latent Infection ◽  
Virus Genome ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein–Barr Virus Infection of Oral Squamous Cells

2020 ◽  
Vol 8 (3) ◽  
pp. 419 ◽  
Author(s):  
Chukkris Heawchaiyaphum ◽  
Hisashi Iizasa ◽  
Tipaya Ekalaksananan ◽  
Ati Burassakarn ◽  
Tohru Kiyono ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epstein Barr Virus ◽  
Squamous Cells ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

The Epstein–Barr virus (EBV) is a human herpesvirus associated with various cancers. The number of reports that describe infection of EBV in oral squamous carcinoma cells is increasing. However, there is no available in vitro model to study the possible role of EBV in the development of oral squamous cell carcinoma. Herein, we report establishment of a latent EBV infection of well-differentiated HSC1 cells and poorly differentiated SCC25 cells. Viral copy numbers per cell in EBV-infected HSC1 and SCC25 cells are 2 and 5, respectively. Although the EBV copy number was small, spontaneous viral replication was observed in EBV-infected HSC1 cells. Contrarily, infectious viral production was not observed in EBV-infected SCC25 cells, despite containing larger number of EBV genomes. Chemical activation of cells induced expression of viral lytic BZLF1 gene in EBV-infected HSC1 cells, but not in EBV-infected SCC25 cells. EBV infection activated proliferation and migration of HSC1 cells. However, EBV-infection activated migration but not proliferation in SCC25 cells. In conclusion, EBV can infect squamous cells and establish latent infection, but promotion of cell proliferation and of lytic EBV replication may vary depending on stages of cell differentiation. Our model can be used to study the role of EBV in the development of EBV-associated oral squamous cell carcinoma.

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