HISTOCHEMICAL COMPARISON OF FROG AND RAT MAST CELLS

Histochemical procedures were used to compare the constituents of frog and rat mast cells. On the basis of staining with acidified toluidine blue and the Alcian Blue-safranin combination, a substance characterized as heparin in rat cells was identified in frog mast cells. In contrast to their presence in rat cells, no histamine, serotonin or catecholamines could be observed in frog mast cells with the o-phthalaldehyde and paraformaldehyde reactions. A search for activities of two proteolytic enzymes, chymotrypsin-like and trypsin-like esterases, in frog mast cells was unsuccessful. β-Glucuronidase, N-acetyl-β-d-glucosaminidase and nonspecific esterase activities were demonstrated in both frog and rat mast cells; acid phosphatase activity was detected in frog but not rat mast cells.

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DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY

The Journal of Cell Biology ◽

10.1083/jcb.25.1.23 ◽

1965 ◽

Vol 25 (1) ◽

pp. 23-41 ◽

Cited By ~ 40

Author(s):

Lois Withrow Tice ◽

Russell J. Barrnett

Keyword(s):

Electron Microscope ◽

Acid Phosphatase ◽

Phosphatase Activity ◽

Alcian Blue ◽

Azo Dyes ◽

Acid Phosphatase Activity ◽

Fixed Material

This paper reports the synthesis of 14 diazophthalocyanins containing Mg, Cu, or Pb as the chelated metal. To assess the usefulness of these compounds for fine structural cytochemistry, the relative coupling rates with naphthols were tested as well as the solubility of the resulting azo dyes. Three of the diazotates were reacted with tissue proteins in aldehyde-fixed material, and the density increases thus produced were compared in the electron microscope with those produced by staining similarly fixed material with the phthalocyanin dye, Alcian Blue. Finally, one of the diazotates was used as a capture reagent for the demonstration of the sites of acid phosphatase activity with the electron microscope.

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Acid Phosphatase Activity of Intramuscular Mast Cells from Cattle and Sheep

Zentralblatt für Veterinärmedizin Reihe A ◽

10.1111/j.1439-0442.1978.tb00956.x ◽

2010 ◽

Vol 25 (7) ◽

pp. 556-561 ◽

Cited By ~ 1

Author(s):

H. J. Swatland

Keyword(s):

Acid Phosphatase ◽

Mast Cells ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Cattle And Sheep

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Muscle biopsy in Pompe disease

Arquivos de Neuro-Psiquiatria ◽

10.1590/0004-282x20130022 ◽

2013 ◽

Vol 71 (5) ◽

pp. 284-289 ◽

Cited By ~ 17

Author(s):

Lineu Cesar Werneck ◽

Paulo José Lorenzoni ◽

Cláudia Suemi Kamoi Kay ◽

Rosana Herminia Scola

Keyword(s):

Acid Phosphatase ◽

Phosphatase Activity ◽

Muscle Biopsy ◽

Pompe Disease ◽

Acid Phosphatase Activity ◽

Glycogen Storage ◽

Nonspecific Esterase ◽

Adult Form ◽

Almost All

Pompe disease (PD) can be diagnosed by measuring alpha-glucosidase levels or by identifying mutations in the gene enzyme. Muscle biopsies can aid diagnosis in doubtful cases.Methods:A review of muscle biopsy from 19 cases of PD (infantile, 6 cases; childhood, 4 cases; and juvenile/adult, 9 cases).Results:Vacuoles with or without glycogen storage were found in 18 cases. All cases had increased acid phosphatase activity. The vacuole frequency varied (almost all fibers in the infantile form to only a few in the juvenile/adult form). Atrophy of type 1 and 2 fibers was frequent in all forms. Atrophic angular fibers in the NADH-tetrazolium reductase and nonspecific esterase activity were observed in 4/9 of the juvenile/adult cases.Conclusion:Increased acid phosphatase activity and vacuoles were the primary findings. Most vacuoles were filled with glycogen, and the adult form of the disease had fewer fibers with vacuoles than the infantile or childhood forms.

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Erythrophagocytosis in the Liver in Hemolytic Anemias

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100664 ◽

1968 ◽

Vol 26 ◽

pp. 184-185

Author(s):

O. T. Minick ◽

E. Orfei ◽

F. Volini ◽

G. Kent

Keyword(s):

Acid Phosphatase ◽

Oxidative Damage ◽

Phosphatase Activity ◽

Kupffer Cells ◽

Acid Phosphatase Activity ◽

Common Type ◽

Red Cell ◽

Cell Fragments ◽

Reticulo Endothelial System ◽

Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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