N-Acetylcysteine Interference with a Glucose Dehydrogenase Linked Glucose Meter

2021 ◽  
pp. 193229682199941
Author(s):  
Martha E. Lyon ◽  
Andrew W. Lyon
Keyword(s):  
Linear Regression ◽  
Clinical Laboratory ◽  
Comparative Method ◽  
Glucose Dehydrogenase ◽  
Gas Analyzer ◽  
Laboratory Methods ◽  
Laboratory Results ◽  
Glucose Meter ◽  
Roche Diagnostics ◽  
Blood Gas Analyzer

Background: Our objective was to determine the effect of therapeutic concentrations of N-acetylcysteine, following intravenous infusion, on the measurement of blood glucose using a Roche Diagnostics glucose dehydrogenase-linked glucose meter compared to hospital laboratory methods. Methods: N-acetylcysteine was added to aliquots of blood, with glucose promptly measured by the glucose meter, blood gas analyzer (glucose oxidase comparative method) and following centrifugation, plasma glucose measured with a hexokinase spectrophotometric comparative method. Glucose results were evaluated with linear regression and Bland Altman plots. Results: In the presence of NAC, at concentrations greater than 5 mg/dL (0.31 mmol/L), positively biased glucose meter results were compared to the clinical laboratory results. Multivariate linear regression revealed that NAC-mediated meter results are influenced by NAC and glucose concentrations. Conclusions: The addition of therapeutic concentrations of NAC to blood produces statistically significant positive biases when measured with the glucose dehydrogenase linked glucose meter device.

Variability of total cholesterol concentrations in serum by repeated measurements in a large pediatric population--limitations of quality controls for laboratory analyses.

1981 ◽  
Vol 27 (12) ◽  
pp. 1988-1992 ◽  
Author(s):  
M C Sklov ◽  
S R Srinivasan ◽  
L S Webber ◽  
G S Berenson
Keyword(s):  
Disease Control ◽  
Total Cholesterol ◽  
Clinical Laboratory ◽  
Pediatric Population ◽  
Large Population ◽  
Repeated Measurements ◽  
Close Monitoring ◽  
Laboratory Methods ◽  
Laboratory Results ◽  
Centers For Disease Control

Abstract We evaluated the variability in total cholesterol concentrations in serum in a large population of children over a period of time, to help us discern the limitations in reliability of current clinical laboratory methods for its analysis. We analyzed sera from a population of approximately 4000 children over a four-year period, quality-control sera, pooled sera, and surveillance samples from the Centers for Disease Control over a seven-year period, with a Technicon AutoAnalyzer II. Two methods of correction were suggested to adjust deviations in yearly serum total cholesterol means: yearly screening data were corrected on the basis of monthly variability in pooled-serum determinations and on the basis of deviations from values suggested by the Centers for Disease Control. Both correction methods were insufficient. These changes occur very slowly, and unless there is close monitoring and frequent comparison with a known standard, changes in laboratory results still can occur.

scholarly journals Comparison of Arterial Blood Gas Analysis versus Central Laboratory Electrolyte and Hemoglobin Determination in Birat Medical College and Teaching Hospital

2020 ◽  
Vol 5 (1) ◽  
pp. 960-963
Author(s):  
Batsalya Arjyal ◽  
Lalit Kumar Rajbanshi ◽  
Kanak Khanal ◽  
Akriti Bajracharya
Keyword(s):  
Clinical Laboratory ◽  
Critically Ill Patient ◽  
Blood Gas ◽  
Central Laboratory ◽  
Gas Analyzer ◽  
Sodium Potassium ◽  
Arterial Blood Gas ◽  
Arterial Blood ◽  
Blood Gas Analyzer ◽  
Hemoglobin Measurement

Introduction: Electrolyte and hemoglobin measurement are the integral part of management of critically ill patient. There can be a wide variation in the electrolyte and hemoglobin measurement in critically ill patient between arterial blood gas analyzer and central laboratory auto analyzer. Objective: To compare the electrolytes (sodium, potassium and chloride) and hemoglobin level measured by arterial blood gas analyzer and laboratory analyzer. Methodology: This was a prospective cross-sectional comparative study comparing the electrolytes (sodium, potassium and chloride) and hemoglobin measurement between arterial blood gas analyzer and laboratory auto analyzer. The study included 124 paired blood samples from the patient admitted in intensive care unit of Birat Medical College Teaching Hospital in two months duration. The arterial sample and venous sample for electrolytes and hemoglobin measurement were taken simultaneously or not more than one hour apart and analysis was done by arterial blood gas analyzer and central laboratory auto analyzer accordingly. The values of electrolytes and hemoglobin measured by two different analyzers were finally compared for variation. Result: The mean difference calculated for sodium potassium and chloride in ABG machine and Auto-analyzers were 0.57 mmol/l.-0.04mmol/l and 1.71mmol/l respectively. These data were within the acceptable range of United States Clinical Laboratory Improvement Amendments(USCLIA). The mean difference derived for hemoglobin in ABG and Auto-analyzers was 0.16g/dl which was not consistent with the range of United States Clinical Laboratory Improvement Amendments (USCLIA) Conclusion: The measurement of electrolyte namely sodium, potassium and chloride in ABG machines and Auto-analyzers of central lab were comparable while hemoglobin was not comparable under the USCLIA guidelines.

scholarly journals Update and clinical management of anti-DNA auto-antibodies

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Concepción González Rodríguez ◽  
MªBelén Aparicio Hernández ◽  
Inmaculada Alarcón Torres
Keyword(s):  
Lupus Erythematosus ◽  
Analytical Methods ◽  
Clinical Laboratory ◽  
Meta Analysis ◽  
Laboratory Methods ◽  
Wide Variability ◽  
Diagnostic Usefulness ◽  
Dna Antibodies ◽  
Editorial Review

Abstract Objectives Anti-deoxyribonucleic acid (DNA) antibodies in the clinical laboratory are intimately linked to the diagnosis and monitoring of systemic lupus erythematosus (SLE); however, the characteristics of the analytical methods and the properties of the antibodies themselves are heterogeneous. To review the definition and properties of anti-double-stranded anti-DNA (anti-dsDNA) antibodies, the adequacy of analytical methods, and the clinical requirements for this biomarker. Content Through PubMed we searched the existing literature with the terms anti-dsDNA, editorial, review, guideline, meta-analysis and SLE. The last search, anti-dsDNA and SLE restricted to the last two years. Information was expanded through related articles and those published in official state bodies related to anti-dsDNA and SLE. Summary Clinical laboratory methods for anti-dsDNA analysis and their characteristics are analyze. The clinical utility of anti-dsDNA in its diagnostic, clinical association and follow-up aspects of SLE is reviewed. Outlook There is wide variability in analytical methods and deficits in standardization persist. They are part of the current SLE classification criteria and are used as markers in the follow-up of the disease. Their diagnostic usefulness improves when they are determined in antinuclear antibody (ANA)-positive patients. In follow-up, quantification is of interest, preferably with the same analytical method (given the deficits in standardization).

Use of a Sodium Chloride—Phosphate Buffer for pH Standardization in a New Blood-Gas Analyzer with an Isotonic Sodium Chloride Bridge

1973 ◽  
Vol 19 (11) ◽  
pp. 1243-1247 ◽  
Author(s):  
P A Drinker ◽  
D C Noonan ◽  
N Ramanaiah ◽  
J R Tole
Keyword(s):  
Sodium Chloride ◽  
Blood Gas ◽  
Gas Analyzer ◽  
Liquid Junction ◽  
Isotonic Sodium Chloride ◽  
Salt Depletion ◽  
The Stability ◽  
Conventional Scheme ◽  
Blood Gas Analyzer ◽  
Junction Potential

Abstract Two different blood-gas analyzers were tested to determine the effects on blood pH measurement of changing the reference bridge solution from saturated KCl to normal saline (0.16 mol of NaCl per liter). This change, which necessitated the preparation of modified buffers equimolal in NaCl with respect to blood, virtually eliminated salt depletion of the bridge solution and improved the stability of the liquid-junction potential between the bridge solution and the sample. The instruments we used were the Corning 165 pH Blood Gas Analyzer and the Radiometer E5021 pH Electrode with PHM72 Acid Base Analyzer. Comparison of results on clinical blood samples indicates that performance with the modified bufferbridge system is the same as that obtained with the conventional scheme. Analytical performances of the Corning and Radiometer instruments for PO2 and PCO2, as well as for pH, were comparable.

ENVIRONMENTAL COMPATIBILITY AND ACCURACY OF A PORTABLE BLOOD GAS ANALYZER IN THE PREHOSPITAL SETTING

1995 ◽  
Vol 23 (Supplement) ◽  
pp. A37
Author(s):  
Bartholomew Tortella ◽  
Robert Lavery ◽  
James Doran ◽  
John Seigel
Keyword(s):  
Prehospital Setting ◽  
Blood Gas ◽  
Gas Analyzer ◽  
Environmental Compatibility ◽  
Blood Gas Analyzer

scholarly journals Effect of Acute Ramadan Fasting on Muscle Function and Buffering System of Male Athletes

Healthcare ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 397
Author(s):  
Mohamad Fashi ◽  
Sajad Ahmadizad ◽  
Hadi Nobari ◽  
Jorge Pérez-Gómez ◽  
Rafael Oliveira ◽  
...  
Keyword(s):  
Muscle Function ◽  
Venous Blood ◽  
Average Power ◽  
Peak Torque ◽  
Buffering Capacity ◽  
Gas Analyzer ◽  
Borg Scale ◽  
Male Athletes ◽  
Ramadan Fasting ◽  
Blood Gas Analyzer

The aim of this study was to investigate the effect of acute Ramadan fasting (RF) on the muscle function and buffering system. Twelve male athletes with 8 years of professional sports experience (age, 23.2 ± 1.3 years, body mass index: 24.2 ± 2.2 kg/m2) participated in this study. The subjects were tested twice, 3 weeks after the beginning of RF and 2 weeks after the end RF. Muscle function, buffering capacity, and rating of perceived exertion (RPE) were measured during and after RF by using the Biodex isokinetic machine, blood gas analyzer, and RPE 6–20 Borg scale, respectively. Venous blood samples for pH and bicarbonate (HCO3−) were measured during and after RF by using the Biodex isokinetic machine, blood gas analyzer, and RPE 6–20 Borg scale, respectively. Venous blood samples for pH and bicarbonate (HCO3−) were taken immediately after 25 repetitions of isokinetic knee flexion and extension. Measures taken during isokinetic knee extension during RF were significantly lower than those after RF in extension peak torque (t = −4.72, p = 0.002), flexion peak torque (t = −3.80, p = 0.007), extension total work (t = −3.05, p = 0.019), extension average power (t = −4.20, p = 0.004), flexion average power (t = −3.37, p = 0.012), blood HCO3− (t = −2.02, p = 0.041), and RPE (Z = −1.69, p = 0.048). No influence of RF was found on the blood pH (t = 0.752, p = 0.476). RF has adverse effects on muscle function and buffering capacity in athletes. It seems that a low-carbohydrate substrate during RF impairs muscle performance and reduces the buffering capacity of the blood, leading to fatigue in athletes.

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