Analysis of Major Chemical Constituents in Luan-Pao-Prescription Using Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrometry

Luan-Pao-Prescription is a famous Chinese herbal formula, which is commonly used for the treatment of female sterility in clinical practice in China. In the present paper, a reliable method based on liquid chromatography coupled with electrospray ionization tandem mass spectrometry in both positive and negative ion modes has been established for the analysis of major chemical constituents in Luan-Pao-Prescription. A total of 34 compounds were either identified or tentatively characterized. These compounds include flavonoids, anthraquinones, iridoids, xanthones and organic acids. Flavonoids were the major constituents of the formula. The results profiled the chemical composition of Luan-Pao-Prescription comprehensively for the first time.

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Monitoring of N-nitrosodiethanolamine in cosmetic products by ion-pair complex liquid chromatography and identification with negative ion electrospray ionization mass spectrometry

Journal of Chromatography A ◽

10.1016/j.chroma.2007.12.045 ◽

2008 ◽

Vol 1185 (1) ◽

pp. 43-48 ◽

Cited By ~ 22

Author(s):

Alireza Ghassempour ◽

Monireh Abbaci ◽

Zahra Talebpour ◽

Bernhard Spengler ◽

Andreas Römpp

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Ion Pair ◽

Negative Ion ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Cosmetic Products ◽

Complex Liquid

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Analysis of Flavonoids in Dalbergia odorifera by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry

Molecules ◽

10.3390/molecules25020389 ◽

2020 ◽

Vol 25 (2) ◽

pp. 389 ◽

Cited By ~ 3

Author(s):

Xiangsheng Zhao ◽

Shihui Zhang ◽

Dan Liu ◽

Meihua Yang ◽

Jianhe Wei

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Electrospray Ionization ◽

Negative Ion ◽

Ionization Mass ◽

Tandem Mass ◽

Relative Standard ◽

Limit Of Quantitation ◽

Dalbergia Odorifera

Dalbergia odorifera, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in D. odorifera. In this paper, a rapid and sensitive ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 17 flavonoids in D. odorifera. Quantification was performed by multiple reaction monitoring using electrospray ionization in negative ion mode. Under the optimum conditions, calibration curves for the 17 analytes displayed good linearity (r2 > 0.9980). The intra- and inter-day precisions (relative standard deviations) were lower than 5.0%. The limit of quantitation ranged from 0.256 to 18.840 ng/mL. The mean recovery range at three spiked concentrations was 94.18–101.97%. The validated approach was successfully applied to 18 samples of D. odorifera. Large variation was observed for the contents of the 17 analytes. Sativanone and 3′-O-methylviolanone were the dominant compounds. The fragmentation behaviors of six flavonoids were investigated using UPLC with quadrupole time-of-flight tandem mass spectrometry. In negative ion electrospray ionization mass spectrometry, all the flavonoids yielded prominent [M − H]− ions. Fragments for losses of CH3, CO, and CO2 were observed in the mass spectra. Formononetin, liquiritigenin, isoliquiritigenin, sativanone, and alpinetin underwent retro-Diels–Alder fragmentations. The proposed method will be helpful for quality control of D. odorifera.

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