scholarly journals Composition and Antibacterial Activity of Essential Oils from Leaf, Stem and Root of Chrysanthemum parthenium (L.) Bernh. from Iran

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat ◽  
Hajar Sadeghi ◽  
Khodamali Oji
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Salmonella Typhi ◽  
Inhibitory Effects ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Microdilution Broth

The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-β-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were α-pinene (50.0%), trans-β-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the microdilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.

scholarly journals Antibacterial Activity and Composition of Essential Oils from Flower, Leaf and Stem of Chaerophyllum Macropodum Boiss. from Iran

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Inhibitory Effects ◽  
Leaf Oil ◽  
Main Components ◽  
Microdilution Broth

The hydrodistilled essential oils from flower, leaf and stem of Chaerophyllum macropodum Boiss. were analyzed by GC and GC/MS. The flower oil was characterized by high amounts of myristicin (42.5%) and trans-β-ocimene (41.0%). In the leaf oil eighteen constituents, representing 99.3% of the total, were identified of which trans-β-ocimene (24.9%), myristicin (15.7%), terpinolene (14.5%), fenchyl acetate (13.9%), cis-β-ocimene (6.3%) and sabinene (6.1%) were major components. The main components of the stem oil were trans-β-ocimene (54.2%), myristicin (22.4%) and sabinene (8.9%). Antibacterial activities of the flower, leaf and stem oils were evaluated using the microdilution broth method. Inhibitory effects on Escherichia coli and Salmonella typhi were recorded.

scholarly journals ANTIBACTERIAL ACTIVITY OF AGASTACHE AURANTIACA ESSENTIAL OILS

2017 ◽  
pp. 63-70 ◽  
Author(s):  
Наталья (Natal'ja) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽  
Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽  
Татьяна (Tat'jana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽  
Анна (Anna) Геннадьевна (Gennad'evna) Шутова (Shutova) ◽  
Виктор (Viktor) Николаевич (Nikolaevich) Леонтьев (Leontiev)
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Gas Liquid Chromatography ◽  
Oil Components ◽  
The Republic ◽  
Main Components

The essential oil obtained by hydrodistillation method from plants of three varieties Agastache aurantiaca, cultivated on the Republic of Belarus were investigated. Some morphobiometric parameters of plants 'Tango', 'Apricot Sprite' and 'Fragrant Delight' and oil yields were determined. Using the technique of gas-liquid chromatography essential oil components were identified and determined. The main components of the essential oil from 'Tango' and 'Fragrant Delight' plants were menton (~ 53 and ~ 65 v/v % respectively) and pulegon (~ 36 and ~ 25 v/v % respectively). The samples of 'Apricot' Sprite' essential oil were rich in isomentone (~ 46 v/v %) and pulegon (~ 41 v/v %). All tested essential oils contained (+)-menton and (+)-pulegon enantiomeric forms only. The antibacterial activity of dimethylsulfoxide and ethanolic essential oil solutions against Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruginosa was proved. Ethanol solutions of essential oils at the concentrations 0.001–0.1 v/v % had significant bactericidal activity. A correlation between the composition of the essential oil and their antibacterial properties was established.

scholarly journals Nepetalactone Content and Antibacterial Activity of the Essential Oils from Different Parts of Nepeta persica

2010 ◽  
Vol 5 (4) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ali Shafaghat ◽  
Khodamali Oji
Keyword(s):  
Essential Oils ◽  
Antibacterial Property ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
The Other ◽  
Inhibitory Effects ◽  
Leaf Oil ◽  
Main Component ◽  
Microdilution Broth ◽  
Different Parts

The essential oils from the flower, leaf, stem and root of Nepeta persica Boiss., analyzed by GC and GC/MS, were shown to contain 4aβ,7α,7aβ-nepetalactone (58.5%, 62.3%, 66.2% and 27.1%, respectively), and 4aα,7α,7aβ-nepetalactone (33.0%, 28.3%, 24.9% and 7.6%, respectively). The other main component of the flower and stem oils was α-pinene (3.6% and 4.4%) and of the leaf oil β-ocimene (3.6%). In the root oil, other main constituents were α-pinene (40.4%), α-amorphene (5.3%), γ-cadinene (2.9%), and cis-calamenene (2.5%). Nepetalactone was the major component of the flower, leaf and stem oils, which are thus important sources of nepetalactone. Antibacterial activities of the flower, leaf, stem and root oils were evaluated using the microdilution broth method. Inhibitory effects on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Enterococcus faecalis were recorded. The flower, leaf, stem, and root oils had difference activities against the test microorganisms. The antibacterial property of the essential oils might be ascribed to their high content of nepetalactone isomers.

scholarly journals Chemical Composition and Antibacterial Activity of Cupressus dupreziana A. Camus

2007 ◽  
Vol 2 (9) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Messaoud Ramdani ◽  
Oualida Rached ◽  
Hocine Laouer ◽  
Meriem El Koli ◽  
Takia Lograda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Diffusion Method ◽  
Germacrene D ◽  
And Staphylococcus Aureus

The essential oils obtained by hydrodistillation from the green branchlets of Cupressus dupreziana A. Camus growing wild in Tassili n'Ajjer (Algeria) were analyzed by GC and GC/MS. The main constituents of the essential oils were α-pinene (27.2% - 44.2%), germacrene D (16.2% - 27.2%) and Δ3-carene (14.2% - 26.7%). The oils showed insignificant activity on the growth of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923), when investigated by the diffusion method.

scholarly journals Chemical Composition and In Vitro Antibacterial Activity of the Essential Oil of Phthirusa adunca from Venezuelan Andes

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Flor D. Mora ◽  
Nurby Ríos ◽  
Luis B. Rojas ◽  
Tulia Díaz ◽  
Judith Velasco ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Characterization ◽  
Salmonella Typhi ◽  
Germacrene D ◽  
In Vitro Antibacterial Activity

In this paper, preliminary studies on the chemical characterization of Phthirusa adunca Meyer essential oil, obtained by hydrodistillation, is presented. The separation of the components was performed by GC-MS. Twenty-three compounds (94.5% of the sample) were identified of which the three major ones (76% of the sample) were β-phellandrene (38.1%), germacrene D (26.8%) and β-pinene (11.5%). The essential oil showed a broad spectrum of activity against Salmonella Typhi CDC 57 (100 μg/mL), Staphylococcus aureus ATCC 25923 (200 μg/mL), Enterococcus faecalis ATCC 29212 (250 μg/mL), Escherichia coli ATCC 25922 y Klebsiella pneumoniae ATCC 23357 (500 μg/mL). This is the first report on the composition and activity of the essential oil of this species.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Composition of the Essential Oils and Antibacterial Activities of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha Three Labiatae Herbs Growing Wild in Iran

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Shiva Masoudi ◽  
Abdolhossein Rustaiyan ◽  
Razieh Mohebat ◽  
Mohammad Hossein Mosslemin
Keyword(s):  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Inhibitory Zone ◽  
Growth Inhibitory ◽  
Germacrene D ◽  
Leaf Oil ◽  
Main Components ◽  
Total Oil ◽  
Stem Leaf

Water-distilled essential oils from leaves of Hymenocrater yazdianus Rech.f., flowers of Stachys obtusicrena Boiss., and stems and flowers of Nepeta asterotricha Rech.f, which are endemic to Iran, were analyzed by GC and GC/MS. Fifty-five components of the leaf oil of H. yazdianus were characterized, representing 95.1% of the total components detected. The major constituents were identified as 1,8-cineole (17.6%), β-caryophyllene (13.9%), α-pinene (10.6%) and caryophyllene oxide (10.4%). Germacrene-D (37.5%) and α-bisabolol (23.5%) were the main components among the twenty constituents characterized in the flower oil of S. obtusicrena, representing 90.8% of the total components detected. Thirty-five compounds representing 93.0% of the stem oil of N. asterotrica were identified among which terpinen-4-ol (22.8%) and γ-terpinene (14.1%) were the major ones. The flower oil of the species was characterized by higher amounts of terpinen-4-ol (24.8%), 4aα, 7aβ-nepetalactone (18.2%) and 1,8-cineole (11.6%) among the thirty-three components comprising 98.5% of the total oil detected. The antibacterial activity of the stem, leaf and flower oils of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha against seven Gram-positive and Gram-negative bacteria were determined using the MIC method. The growth inhibitory zone (mm) was also measured.

scholarly journals Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils

2019 ◽  
pp. 1-11
Author(s):  
Mahamat Ismail ◽  
Veronique Penlap ◽  
Wilfred F. Mbacham ◽  
Sylvain Leroy Sado Kamdem
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Essential Oils ◽  
Salmonella Typhi ◽  
World Health ◽  
Partial Substitution ◽  
Piper Nigrum ◽  
Panel Test ◽  
One Year

Aim: This work was carried out in order to reduce the isopropanol contain of a World Health Organization (WHO) basic alcohol based hand rub (ABHR) formulation by substituting part of it with ethanol and essential oils. Study Design: A quasi-experimental design was used, based on WHO basic formulation modification, antimicrobial assessment followed by a panel test and challenge test of the best formulation. Place and Duration of the Study: The study was carried out in different laboratory of the University of Yaoundé 1 during March 2017 to July 2018. Methodology: This study was performed by producing different formulations nested from the WHO basic formulation and testing their antimicrobial capacity on selected strains based on the microbial percentage reduction. Following this, the best formulation was compared to two commercial products trough a panel test and later challenged with selected organism (Staphylococcus aureus SR196, Salmonella Typhi 15SA, Escherichia coli ATTC25922 and Pseudomonas aeruginosa PA01) inoculated in the product after different storage periods for one year and the antimicrobial stability assessed on the same strain for the same length of conservation. Results: The partial substitution of isopropanol with ethanol and Piper nigrum and Syzygium aromaticum essential oils proved to possess more antimicrobial properties than the original WHO formulation. The best formulation caused a 6 Log cells/ml reduction of the initial population compared to the 4 Log cells/ml of the WHO formulation. The product also proved to maintain its activity for one year and to be able to deactivate possible contaminations by Salmonella Typhi 15SA, Staphylococcus aureus NCTC10652, Escherichia coli ATCC25922, Pseudomonas aeruginosa PA01. Conclusion: The present work is a contribution to the improvement of ABHRs and could permit the reduction of hand hygiene associated infections in industries and health care facilities.

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