A New Bioactive Secondary Metabolite from Artocarpus elasticus

Detailed phytochemical investigation has been carried out on the bark of Artocarpus elasticus Reinw. ex Blume, which led to the isolation of artonin E (1), a new dihydrobenzoxanthone derivative named elastixanthone (2), cycloartobiloxanthone (3) and artobiloxanthone (4). Structures of these compounds were elucidated on the basis of various spectroscopic (UV, IR, 1D-NMR and 2D-NMR) and MS data. Compounds 1-3 displayed outstanding scavenging activity for 1,1-diphenylpicrylhydrazyl (DPPH) with IC50 values of 11.5, 21.6 and 40.0 μg/mL, respectively. In addition, compounds 1-3 displayed broad spectrum antimicrobial activities against thirteen different bacterial strains when tested using the disc diffusion assay. Cytotoxic screening revealed that artonin E (1) constantly exhibited strong cytotoxic activity against human estrogen receptor (ER+) positive breast cancer (MCF-7) and human estrogen receptor (ER-) negative (MDA-MB 231) cells in comparison with the other two, with IC50 values of 2.6 and 13.5 μg/mL, respectively, without being toxic towards the WRL68 (human normal liver) cell line (IC50 value more than 30 μg/mL). However, the compound was inactive against HepG2 (human liver carcinoma) cancer cells.

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Two highly acetylated sterols from the marine sponge Dysidea sp.

Zeitschrift für Naturforschung B ◽

10.1515/znb-2016-0156 ◽

2017 ◽

Vol 72 (1) ◽

pp. 49-52 ◽

Cited By ~ 4

Author(s):

Yu Lu ◽

Ming Zhao

Keyword(s):

High Resolution ◽

Mass Spectroscopy ◽

2D Nmr ◽

Antimicrobial Activities ◽

Marine Sponges ◽

Bacterial Strains ◽

Chemical Structures ◽

Spectroscopic Analyses ◽

Impact Mass

AbstractTwo new highly acetylated steroids, named dysiroid A (1) and dysiroid B (2), together with a known compound (3) were isolated from the marine sponges Dysidea sp. The chemical structures of 1 and 2 were elucidated by spectroscopic analyses including 1D and 2D NMR experiments and high-resolution electrospray impact mass spectroscopy. Their in vitro antimicrobial activities against a panel of bacterial strains were evaluated. Compounds 1 and 2 showed potent activity against some of the strains with minimum inhibitory concentrations ranging from 4 to 8 μg mL−1.

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Antimicrobial and Antioxidant Polyketides from a Deep-Sea-Derived Fungus Aspergillus versicolor SH0105

Marine Drugs ◽

10.3390/md18120636 ◽

2020 ◽

Vol 18 (12) ◽

pp. 636

Author(s):

Lu-Jia Yang ◽

Xiao-Yue Peng ◽

Ya-Hui Zhang ◽

Zhi-Qing Liu ◽

Xin Li ◽

...

Keyword(s):

Deep Sea ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

2D Nmr ◽

Antimicrobial Activities ◽

Aspergillus Versicolor ◽

Bacterial Strains ◽

Antioxidant Power ◽

Norsolorinic Acid ◽

Ferric Reducing Antioxidant Power

Fifteen polyketides, including four new compounds, isoversiol F (1), decumbenone D (2), palitantin B (7), and 1,3-di-O-methyl-norsolorinic acid (8), along with 11 known compounds (3–6 and 9–15), were isolated from the deep-sea-derived fungus Aspergillus versicolor SH0105. Their structures and absolute configurations were determined by comprehensive spectroscopic data, including 1D and 2D NMR, HRESIMS, and ECD calculations, and it is the first time to determine the absolute configuration of known decumbenone A (6). All of these compounds were evaluated for their antimicrobial activities against four human pathogenic microbes and five fouling bacterial strains. The results indicated that 3,7-dihydroxy-1,9-dimethyldibenzofuran (14) displayed obvious inhibitory activity against Staphylococcus aureus (ATCC 27154) with the MIC value of 13.7 μM. In addition, the antioxidant assays of the isolated compounds revealed that aspermutarubrol/violaceol-I (15) exhibited significant 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity with the IC50 value of 34.1 μM, and displayed strong reduction of Fe3+ with the ferric reducing antioxidant power (FRAP) value of 9.0 mM under the concentration of 3.1 μg/mL, which were more potent than ascorbic acid.

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Exon deletions and variants of human estrogen receptor mRNA in endometrial hyperplasia and adenocarcinoma

International Journal of Gynecological Cancer ◽

10.1046/j.1525-1438.2000.00009.x ◽

2000 ◽

Vol 10 (2) ◽

pp. 128-136 ◽

Cited By ~ 16

Author(s):

G. Horvath ◽

G. Leser ◽

M. Hahlin ◽

M. Henriksson

Keyword(s):

Estrogen Receptor ◽

Endometrial Hyperplasia ◽

Receptor Mrna ◽

Estrogen Receptor Mrna ◽

Human Estrogen Receptor

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Antimicrobial Activity and Chemical Characterization of a Non-Polar Extract of Saffron Stamens in Food Matrix

Foods ◽

10.3390/foods10040703 ◽

2021 ◽

Vol 10 (4) ◽

pp. 703

Author(s):

Severino Zara ◽

Giacomo L. Petretto ◽

Alberto Mannu ◽

Giacomo Zara ◽

Marilena Budroni ◽

...

Keyword(s):

Chemical Composition ◽

Fatty Acid Content ◽

Chemical Characterization ◽

Antimicrobial Activities ◽

Added Value ◽

Gas Chromatography Mass Spectrometry ◽

Food Matrix ◽

Bacterial Strains ◽

By Products

The production of saffron spice generates large quantities of plant by-products: over 90% of the plant material collected is discarded, and a consideration fraction of this waste is plant stamens. This work investigated the chemical composition and the antimicrobial activities of the non-polar fraction extracted from four different saffron flower stamens. The chemical composition of ethereal extracts of the saffron stamens was qualitatively assessed by means of gas–chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses. These analyses revealed ethereal extracts to possess a high polyunsaturated fatty acid content. In vitro antibacterial activity of stamen extracts showed no large differences between Gram-positive and Gram-negative bacteria in terms of minimal inhibitory concentration (MIC). In food matrix microbial analysis of the bacterial strains belonging to the main foodborne pathogen species, including Staphylococcus aureus DSM 20231, Escherichia coli DSM 30083, and Listeria monocytogenes DSM 20600, using low-fat UHT milk, revealed a statistically significant reduction in the number of cells (particularly for E. coli and S. aureus with a complete elimination of the population of the two target bacteria following incubation in diethyl ether extracts of saffron stamen (DES) at high concentrations tested, both at 37 °C and 6 °C (for 48 h and 7 days, respectively). A synergic effect was observed when the pathogens were incubated at 6 °C with DES. This work shows these by-products to be excellent sources of bioactive compounds, which could be exploited in high-added-value products, such as food, cosmetics, and drugs.

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Mutagenesis of cysteines in the hormone binding domain of the human estrogen receptor. Alterations in binding and transcriptional activation by covalently and reversibly attaching ligands

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)99101-5 ◽

1991 ◽

Vol 266 (17) ◽

pp. 10880-10887 ◽

Cited By ~ 2

Author(s):

J.C. Reese ◽

B.S. Katzenellenbogen

Keyword(s):

Estrogen Receptor ◽

Transcriptional Activation ◽

Binding Domain ◽

Hormone Binding ◽

Human Estrogen Receptor ◽

Hormone Binding Domain

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Selective Anti-Cancer Effects of Plasma-Activated Medium and Its High Efficacy with Cisplatin on Hepatocellular Carcinoma with Cancer Stem Cell Characteristics

International Journal of Molecular Sciences ◽

10.3390/ijms22083956 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3956

Author(s):

Yan Li ◽

Tianyu Tang ◽

Hae June Lee ◽

Kiwon Song

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Death ◽

Cell Lines ◽

Primary Liver Cancer ◽

Atmospheric Pressure Plasma ◽

Normal Liver ◽

Normal Liver Cell ◽

Ample Evidence ◽

Huh7 Cells ◽

Anti Cancer

Hepatocellular carcinoma (HCC) is a major histological subtype of primary liver cancer. Ample evidence suggests that the pathological properties of HCC originate from hepatic cancer stem cells (CSCs), which are responsible for carcinogenesis, recurrence, and drug resistance. Cold atmospheric-pressure plasma (CAP) and plasma-activated medium (PAM) induce apoptosis in cancer cells and represent novel and powerful anti-cancer agents. This study aimed to determine the anti-cancer effect of CAP and PAM in HCC cell lines with CSC characteristics. We showed that the air-based CAP and PAM selectively induced cell death in Hep3B and Huh7 cells with CSC characteristics, but not in the normal liver cell line, MIHA. We observed both caspase-dependent and -independent cell death in the PAM-treated HCC cell lines. Moreover, we determined whether combinatorial PAM therapy with various anti-cancer agents have an additive effect on cell death in Huh7. We found that PAM highly increased the efficacy of the chemotherapeutic agent, cisplatin, while enhanced the anti-cancer effect of doxorubicin and the targeted-therapy drugs, trametinib and sorafenib to a lesser extent. These findings support the application of CAP and PAM as anti-cancer agents to induce selective cell death in cancers containing CSCs, suggesting that the combinatorial use of PAM and some specific anti-cancer agents is complemented mechanistically.

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