Preventive Effect of Abietic Acid against Skin Cancer of Mice

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200
Author(s):  
Nianyun Yang ◽  
Lijuan Tian
Keyword(s):  
Skin Cancer ◽  
Immunohistochemical Staining ◽  
Acid Treatment ◽  
Abietic Acid ◽  
Tumor Formation ◽  
Morphological Alteration ◽  
Preventive Effect ◽  
Mice Model ◽  
Obvious Effect

The preventive effect of abietic acid on skin cancer in mice was investigated in this study. Skin cancer mice model was caused by the combination of UVC and DMBA, and abietic acid was meanwhile intervened. The histopathology features of skin dyed HE staining and immunohistochemical staining were observed to explore tumor formation and skin morphological alteration, and MMP-1, MMP-3, CK10 and CK5/6 were examined. Abietic acid treatment had an obvious effect on experimental mice, and decreasing the expression of the above MMPs and CKs might be one of the possible mechanisms of the therapy.

Preventive effect of chemical peeling on ultraviolet induced skin tumor formation

2010 ◽  
Vol 60 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Mohamed Abdel-Daim ◽  
Yoko Funasaka ◽  
Tsuneyoshi Kamo ◽  
Masahiko Ooe ◽  
Hiroshi Matsunaka ◽  
...  
Keyword(s):  
Tumor Formation ◽  
Skin Tumor ◽  
Preventive Effect ◽  
Chemical Peeling

scholarly journals Effect of Artemisia vulgaris Extract on Granzyme Expression and Tumor Mass Diameter (Study of Adriamycin Cyclophosphamide Chemotherapy in Adenocarcinoma Mammae C3H Mice Model)

2021 ◽  
Vol 31 (4) ◽  
pp. 1
Author(s):  
Gery Rifano Hardanto ◽  
Selamat Budijitno ◽  
Hardian Hardian
Keyword(s):  
Breast Cancer ◽  
Immunohistochemical Staining ◽  
Significant Negative Correlation ◽  
Diameter Growth ◽  
Artemisia Vulgaris ◽  
Mice Model ◽  
Tumor Mass ◽  
Therapeutic Modalities ◽  
C3h Mice ◽  
Apoptotic Effects

<p>Breast cancer incident continues to increase globally. The surgical management can be combined with other therapeutic modalities, including chemotherapy, radiation, and immunotherapy, such as Artemisia vulgaris (AV). This study aimed to determine the effect of AV extract on Granzyme expression and tumor mass diameter growth of C3H mice with adenocarcinoma mammae. Twenty-four female C3H mice were randomly divided into groups of K (control), P1 (AC chemotherapy), P2 (AV extract), and P3 (combination). Adenocarcinoma mammae were inoculated from donor mice. Two cycles of chemotherapy by Adriamycin 0.18 mg and Cyclophosphamide 1.8 mg were given intravenously, while Artemisia vulgaris 13 mg (0.2 ml) was given orally once per day. Granzyme expression was assessed using immunohistochemical staining, while tumor mass diameter growth was measured using tumor calipers. There was a significant negative correlation between and tumor mass diameter growth (p=0,001 and r=-0,911). Artemisia vulgaris increases the apoptotic effects of Adriamycin-Cyclophosphamide chemotherapy by increasing Granzyme expression and decreasing tumor mass diameter growth in adenocarcinoma mammae C3H mice.</p>

scholarly journals Opioid-induced hyperalgesia in a mice model of orthopaedic pain: preventive effect of ketamine †

2010 ◽  
Vol 104 (2) ◽  
pp. 231-238 ◽  
Author(s):  
V Minville ◽  
O Fourcade ◽  
J.-P. Girolami ◽  
I Tack
Keyword(s):  
Preventive Effect ◽  
Mice Model ◽  
Opioid Induced Hyperalgesia

scholarly journals p21-Activated Kinase 1 Is Required for Efficient Tumor Formation and Progression in a Ras-Mediated Skin Cancer Model

2012 ◽  
Vol 72 (22) ◽  
pp. 5966-5975 ◽  
Author(s):  
Hoi Yee Chow ◽  
Adrian M. Jubb ◽  
Jennifer N. Koch ◽  
Zahara M. Jaffer ◽  
Dina Stepanova ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Tumor Formation ◽  
Cancer Model ◽  
P21 Activated Kinase

scholarly journals Chemoprevention of Lung Carcinogenesis by Red Ginseng and Ginsenoside Rg3 in A/J mice

2020 ◽  
Author(s):  
Jie Xiong ◽  
Hongmei Yuan ◽  
Shihong Fei ◽  
Hongge Wu ◽  
Jing Cheng ◽  
...  
Keyword(s):  
Oral Bioavailability ◽  
Lung Tumor ◽  
Tumor Formation ◽  
Mass Spectrometry Analysis ◽  
Lung Carcinogenesis ◽  
Red Ginseng ◽  
Ginsenoside Rg3 ◽  
Preventive Effect ◽  
Korean Red Ginseng ◽  
P Glycoprotein

Abstract Background: Red ginseng has long been used as a traditional medicine for a variety of maladies. Ginsenosides are the active components of ginseng but are limited by their low oral bioavailability. Methods: We evaluated several types of red ginseng extracts for their ability to inhibit lung tumor formation and growth induced by the carcinogen benzo(a)pyrene [B(a)P] in A/J mice. The concentrations of various ginsenosides were quantified in these ginseng extracts using the methods of ultra-performance liquid chromatography tandem mass spectrometry analysis to identify the ginsenosides that may contribute to cancer prevention. We next explored whether inhibition of P-glycoprotein by verapamil could increase the oral bioavailability of ginsenoside by using CaCo-2 cell transcellular transport and in situ mouse intestinal perfusion models. The plasma and intestine concentration of ginsenoside and cancer preventive effect of ginsenoside combined with verapamil in A/J mice were also detected by using B(a)P-induced mouse cancer model.Results: We found that treatment with one type of red ginseng (Korean red ginseng B, KRGB) led to a significant reduction of tumor load compared with other types of red ginseng. KRGB contained the highest concentration of ginsenoside Rg3 among these red ginseng extracts, suggested that Rg3 may play an important role in its preventive efficacy. Our study showed that Rg3 had a relatively poor bioavailability, and co-administration of verapamil decreased the efflux ratio of Rg3 in Caco-2 cells, increased the absorption rate of Rg3 in rat small intestine, increased the plasma and intestine concentration of Rg3 in A/J mice, and enhanced the cancer preventive effect of Rg3 against B(a)P induced lung tumorigenesis. Conclusions: Ginsenosides Rg3 is one of the key components of read ginseng that may be responsible for its efficacy against lung carcinogenesis in mice. Rg3 appears to be the substrate of P-glycoprotein, and inhibition of P-glycoprotein could enhance its oral bioavailability.

Preventive effect of antioxidant on ultraviolet-induced skin cancer in mice

2000 ◽  
Vol 23 ◽  
pp. S45-S50 ◽  
Author(s):  
Masamitsu Ichihashi ◽  
Nazim U Ahmed ◽  
Arief Budiyanto ◽  
An Wu ◽  
Toshinori Bito ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Preventive Effect

Incidence of New Tumor Formation in Patients with Hereditary Retinoblastoma Treated with Primary Systemic Chemotherapy: Is There a Preventive Effect?

Ophthalmology ◽  
2007 ◽  
Vol 114 (11) ◽  
pp. 2077-2082.e1 ◽  
Author(s):  
Matthew W. Wilson ◽  
Barrett G. Haik ◽  
Catherine A. Billups ◽  
Carlos Rodriguez-Galindo
Keyword(s):  
Systemic Chemotherapy ◽  
Tumor Formation ◽  
Preventive Effect ◽  
Primary Systemic Chemotherapy

scholarly journals 5-Demethylnobiletin is more effective than nobiletin in preventing AOM/DSS-induced colorectal carcinogenesis in ICR mice

2018 ◽  
Vol 2 ◽  
Author(s):  
Jia-Ching Wu ◽  
Yen-Chen Tung ◽  
Yu-Nu Zheng ◽  
Mei-Ling Tsai ◽  
Ching-Shu Lai ◽  
...  
Keyword(s):  
Molecular Level ◽  
Biological Activities ◽  
Tumor Formation ◽  
Colorectal Carcinogenesis ◽  
Pcr Analysis ◽  
Mice Model ◽  
Chain Reaction ◽  
Icr Mice ◽  
Polymerase Chain ◽  
Anti Inflammation

Nobiletin and 5-demethylnobiletin as polymethoxyflavone and hydroxy polymethoxyflavone, especially, have been reported to exhibit various beneficial biological activities for human health. In this study, the effects of NOB and 5-demethylnobiletin (DMNB), on azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colorectal carcinogenesis in Institute for Cancer Research (ICR) mice were compared. We found that NOB and DMNB significantly alleviated the weight loss, colon length and reduced colon tumor formation in AOM/DSS-induced colorectal carcinogenesis mice. At the molecular level, our results from western blot and polymerase chain reaction (PCR) analysis showed that 0.025% of NOB and DMNB presented an anti-inflammation property by reducing cyclooxygenase- 2 (COX-2) and interleukin 6 (IL-6) expression. Taken together, these results demonstrate that DMNB had a better chemo-preventive efficacy than NOB in AOM/DSS-induced colorectal carcinogenesis in ICR mice model.

The Establishment of Mice Model with Myelodysplastic Syndrome Cell Line MUTZ-1 Cell.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4911-4911
Author(s):  
Jie Lu ◽  
Jie Jin ◽  
Weilai Xu ◽  
Zhimei Chen ◽  
Wei Ding
Keyword(s):  
Latent Period ◽  
Cell Line ◽  
Arsenic Trioxide ◽  
Tumor Cells ◽  
First Generation ◽  
Abdominal Cavity ◽  
Tumor Formation ◽  
Scid Mice ◽  
Mice Model

Abstract Objective To establish myelodysplastic syndrome cell line MUTZ-1 cell mice model. Methods 75 SCID mice and 10 BALB/CA-node mice were studied in this experiment. MDS-REBT cell line MUTZ-1 cells were cultured in vitro and 1 x108 /ml cell were subcutancously implanted in 4~6-week-old First-Generation SCID mice and BALB/CA-node mice respectively. The subcutancous tumor cells from First-Generation MDS-REBT cell line MUTZ1 cells mice model were respectively implanted in Second-Generation SCID mice and BALB/CA-node mice. The latent period and the rate of subcutancous tumor formation was observed, and tumor size was measured by the MTD twice a week. The biological characteristics of the subcutancous tumor cells were checked and evaluated by the methods of cell morphology and pathology and histopathology and immunology by flow cytometer, chromosome analysis and immunohistochemistry stain. It has been experimented tentatively that arsenic trioxide (AS2O3) (with 7.5mg/g mouse/d x 3d) was injected into abdominal cavity in 10 SCID mice to observe the drug effect on tumor formation in vivo. Results The rate of the subcutancous tumor formation was 98.6% (70/71)in SCID mice and 62.5%(5/8)in BALB/CA-nude micerespectively,(P=0.0027). The latent period of the subcutancous tumor formation were 10~17d (median 12d) in 61 SCID mice without injected arsenic trioxide and were 26~31d (median 29d) in 10 SCID mice with arsenic trioxide (with 7.5mg/g mouse/d x 3d) injected into abdominal cavity respectively. The latent period of the subcutancous tumor formation in the group of SCID mice injected by AS2O3 was significantly longer than that of non-injected by AS2O3(Z=5.339, P&lt;0.001), which showed that AS2O3 has probably a inhibition effect on the subcutancous tumor growth of SCID mice model in vivo. The results showed the subcutancous tumor cells of mice model have the biological characteristics of a Hum-MDS-REBT cell line MUTZ1 cell and were anthropo- source. The metastases in liver were found 8 weeks after implanted. HE staining on paraffin sections showed that the tumor cells were microscopic observed on liver and spleen and kidney and medullary cavity of bone and skin. Immunohistochemistry staining showed the subcutancous tumor cells of the mice model have the overexpression of P53 protein, which cued that these tumor cells has the P53 mutation and showed human tumor propert. Conclusion A human myelodysplastic syndromes-REBT cell line MUTZ1 cell-severe combined immunodeficiency (SCID) mice model was successfully established, which could be used for the advanced investigation of drug experiment as an animal model system in vivo.

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