A New Monoterpenoid Glucoindole Alkaloid From Dipsacus asper

The monoterpenoid glucoindole alkaloids comprise a large class of structurally complex natural products that have attracted widespread attention owing to their biological activities and structural diversity. In the present study, we investigated the constituents of a methanol extract of Dipsacus asper roots and obtained a new monoterpenoid glucoindole alkaloid, (3 R,5 S)-5-carboxyvincosidic acid 22-loganin ester (1), together with the known alkaloid (3 S,5 S)-5-carboxystrictosidic acid 22-loganin ester (dipsaperine) (2). We herein report our structural elucidation of 1 by spectroscopic analysis and its anti-inflammatory activity as revealed by its inhibition of nitric oxide production in lipopolysaccharide-activated RAW264.7 cells.

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Anti-Inflammatory Activity of Methanol Extract and Fractions from Alchemilla kiwuensis Engl. on LPS Activated Macrophages

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i4.8117 ◽

2017 ◽

Vol 9 (4) ◽

Author(s):

Kamtchueng M.O. ◽

Balyan R. ◽

Mouokeu R S ◽

Tume C. ◽

...

Keyword(s):

Nitric Oxide ◽

Methanol Extract ◽

Bioactive Metabolites ◽

Nitric Oxide Production ◽

Activated Macrophages ◽

E Coli ◽

Oxide Production ◽

Whole Plant ◽

Tnf Α ◽

Anti Inflammatory

Medicinal plants have been used to treat different diseases over the world and they constitute an inexhaustible source of bioactive metabolites. Alchemilla kiwuensis is a Cameroonian medicinal plant used for the treatment of various diseases. The present study investigates the anti-inflammatory properties of the methanol extract and fractions on LPS activated macrophages. The extract was prepared by maceration of dry whole plant powder in methanol, following by fractioning using hexane and ethyl acetate. The activity was evaluated on RAW 246.7 cell and bone marrow differentiated macrophages by measuring their effect on nitric oxide production, phagocytosis, cytokine production and cell surface markers. The crude extract and its fractions significantly (P less than 0.05) inhibited nitric oxide production by LPS activated macrophages. As shown by RT-PCR, iNOS enzyme was inhibited in the presence of extract and its fractions. They also significantly (P less than 0.05) inhibited phagocytosis of E. coli and production of TNF-α and IL-6 by LPS activated macrophages. The extracts reduced the expression of the co-stimulatory molecule, CD80. The findings indicate that Alchemilla kiwuensis has potent anti-inflammatory properties.

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Dibenzocyclooctadiene Lignans from Artemisia sieversiana and Their Nitric Oxide Inhibitory Effects

10.3762/bxiv.2020.64.v1 ◽

2020 ◽

Author(s):

Xu-Dong Zhou ◽

Chao-Xi Chen ◽

Xi-Kang Zheng ◽

Li-Min Gong ◽

Ke-Wu Zeng ◽

...

Keyword(s):

Nitric Oxide ◽

Spectroscopic Analysis ◽

Positive Control ◽

Microglial Cells ◽

Moderate Activity ◽

Nitric Oxide Production ◽

Inhibitory Effects ◽

Oxide Production ◽

Aerial Parts ◽

Circular Dichroïsm

Two previously undescribed dibenzocyclooctadiene lignans, named sieverlignans D–E (1-2), as well as eight known ones (3-10), were isolated from the aerial parts of Artemisia sieversiana. Their structures were elucidated from extensive spectroscopic analysis, including HRMS, NMR and circular dichroism experiments. This study is the first to report dibenzocyclooctadiene lignans in the genus Artemisia and this plant. All the compounds were evaluated for their anti-neuroinflammatory effects on the lipopolysaccharides (LPS)-induced nitric oxide production in BV-2 murine microglial cells. Compounds 1 and 6 exhibited the moderate activity with their IC50 values of 47.7 and 21.9 μM, compared to a positive control quercetin with the IC50 value of 16.0 μM.

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