REFINEMENT OF THE BIS-(THIOACETOXY) AURATE (I) METHOD FOR THE ELECTRON MICROSCOPIC LOCALIZATION OF ACETYLCHOLINESTERASE AND NONSPECIFIC CHOLINESTERASE

The bis-(thioacetoxy) aurate (I) histochemical method has been refined to permit reliable electron microscopic localization of acetylcholinesterase and nonspecific cholinesterase in autonomic ganglia and other mammalian and submammalian tissues. The detailed procedure is presented, along with illustrations of its specificity by light microscopy and high degree of resolution by electron microscopy.

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The electron microscopic localization of acetylcholinesterase and pseudocholinesterase in autonomic ganglia

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)32129-8 ◽

1974 ◽

Vol 24 ◽

pp. 11-12

Author(s):

George B. Koelle

Keyword(s):

Autonomic Ganglia ◽

Electron Microscopic ◽

Microscopic Localization ◽

Electron Microscopic Localization

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Inorganic trimetaphosphatase as a histochemical marker for lysosomes in light and electron microscopy.

Journal of Histochemistry & Cytochemistry ◽

10.1177/25.12.200672 ◽

1977 ◽

Vol 25 (12) ◽

pp. 1381-1384 ◽

Cited By ~ 73

Author(s):

S B Doty ◽

C E Smith ◽

A R Hand ◽

C Oliver

Keyword(s):

Electron Microscopy ◽

Incubation Medium ◽

Soft Tissues ◽

Light And Electron Microscopy ◽

Electron Microscopic ◽

Cytochemical Method ◽

Microscopic Localization ◽

Histochemical Marker ◽

Electron Microscopic Localization

A new cytochemical method is presented for the light and electron microscopic localization of lysosomes in mineralized and soft tissues. Inorganic trimetaphosphate is used as substrate in a lead chelate incubation medium at pH 3.9. Lysosomes in several tissues are strongly reactive, and reaction product is frequently present in Golgi saccules and GERL. The reaction can be differentiated from acid glycerophosphatase activity, is relatively insensitive to fixation and demineralization procedures, and the reaction is often complete after short incubation times.

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Electron Microscopic Localization of Russell Bodies in the Human Plasma Cell

Blood ◽

10.1182/blood.v16.3.1307.1307 ◽

1960 ◽

Vol 16 (3) ◽

pp. 1307-1312 ◽

Cited By ~ 17

Author(s):

RONALD A. WELSH

Keyword(s):

Electron Microscopy ◽

Endoplasmic Reticulum ◽

Human Plasma ◽

Plasma Cell ◽

Plasma Cells ◽

Electron Microscopic ◽

Degenerative Process ◽

Microscopic Localization ◽

Electron Microscopic Localization

Abstract The location of Russell bodies in the human plasma cell was shown by electron microscopy to be within the intracisternal space of the endoplasmic reticulum. The significance of this finding was discussed from the standpoint of possible intracellular function of the endoplasmic reticulum. The appearance of the affected plasma cells tended to negate a degenerative process, and the suggestion was offered that the Russell body results from a condensation of intracisternal secretion.

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Immunocytochemistry. A Review of Methodology for Electron Microscopic Applicaiton

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051657 ◽

1974 ◽

Vol 32 ◽

pp. 328-329

Author(s):

Joseph E. Mazurkiewicz

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Ultrastructural Level ◽

Electron Microscopic ◽

Biological Interest ◽

Investigative Approach ◽

Immunologic Activity ◽

Dense Label

Immunocytochemistry is a powerful investigative approach in which one of the most exacting examples of specificity, that of the reaction of an antibody with its antigen, isused to localize tissue and cell specific molecules in situ. Following the introduction of fluorescent labeled antibodies in T950, a large number of molecules of biological interest had been studied with light microscopy, especially antigens involved in the pathogenesis of some diseases. However, with advances in electron microscopy, newer methods were needed which could reveal these reactions at the ultrastructural level. An electron dense label that could be coupled to an antibody without the loss of immunologic activity was desired.

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A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060672 ◽

1967 ◽

Vol 25 ◽

pp. 132-133

Author(s):

R. Stephens ◽

G. Schidlovsky ◽

S. Kuzmic ◽

P. Gaudreau

Keyword(s):

Electron Microscopy ◽

Tissue Culture ◽

Light Microscopy ◽

Cell Sheet ◽

Culture Cell ◽

Tissue Culture Cell ◽

Electron Microscopic ◽

Cell Sheets ◽

Morphological Alterations ◽

Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

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