Titanium implants modified by laser microtexturing enhance the bioactivity of gastric epithelial cells and fibroblast cells

The clinical application of anastomotic instruments improves the efficiency of the digestive tract surgery. However, the stapler with titanium nails implanted is still controversial in terms of anastomotic complications, and further improvement and optimization are needed. The purpose of this study was to explore the optimal microtextured parameters that could enhance the bioactivity of titanium implants in vitro. Laser microtexturing technology was used to construct the groove-type microstructural surfaces with different parameters, and human gastric mucosal epithelial cells (GES-1 cells) and mouse fibroblasts (3T3 cells) were cultured on the surface of the titanium plates in vitro. The data of cell adhesion, cell proliferation and cell activity were obtained and statistically analyzed. The textured titanium plates meet the expected design. GES-1 and 3T3 cell adhesion were better in the surface of titanium plates in microstructural group than that in the polished group. GES-1 and 3T3 cells also showed higher proliferative activity in the microstructural group compared with the polished group. The laser textured titanium plates have good groove-type microstructure, which increase the surface roughness, change the surface wettability, promote the adhesion, proliferating and orderly growth of GES-1 and 3T3 cells, and show good biological properties.

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Tannins from Chestnut (Castanea sativa Mill.) leaves and fruits show promising in vitro antiinflammatory properties in gastric epithelial cells

10.1055/s-0039-3400094 ◽

2019 ◽

Author(s):

S Piazza ◽

E Sangiovanni ◽

U Vrhovsek ◽

M Fumagalli ◽

S Khalilpour ◽

...

Keyword(s):

Epithelial Cells ◽

Castanea Sativa ◽

Gastric Epithelial Cells ◽

Castanea Sativa Mill

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Rabeprazole inhibits inflammatory reaction by inhibition of cell pyroptosis in gastric epithelial cells

BMC Pharmacology and Toxicology ◽

10.1186/s40360-021-00509-7 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Jing Xie ◽

Long Fan ◽

Liya Xiong ◽

Peiyu Chen ◽

Hongli Wang ◽

...

Keyword(s):

Epithelial Cells ◽

Clinical Sample ◽

Critical Role ◽

Enzyme Linked Immunosorbent Assay ◽

Inflammasome Activation ◽

Peptic Ulcers ◽

Gastric Epithelial Cells ◽

Caspase 1 ◽

H Pylori

Abstract Background Helicobacter pylori (H. pylori) is a common pathogen in development of peptic ulcers with pyroptosis. Rabeprazole, a critical component of standard triple therapy, has been widely used as the first-line regimen for H. pylori infectious treatment. The aim of this study to explore the function of Rabeprazole on cell pyroptosis in vitro. Methods The clinical sample from patients diagnosed with or without H. pylori-infection were collected to analyze by Immunohistochemistry (IHC). Real-time quantitative PCR (qPCR), western blot (WB) and enzyme linked immunosorbent assay (Elisa) were performed to analyze the effect of Rabeprazole on cell pyroptosis, including LDH, IL-1β and IL-18. Results In this study, we showed that Rabeprazole regulated a phenomenon of cell pyroptosis as confirmed by lactate dehydrogenase (LDH) assay. Further results showed that Rabeprazole inhibited cell pyroptosis in gastric epithelial cells by alleviating GSDMD-executed pyroptosis, leading to decrease IL-1β and IL-18 mature and secretion, which is attributed to NLRP3 inflammasome activation inhibition. Further analysis showed that ASC, NLRP3 and Caspase-1, was significantly repressed in response to Rabeprazole stimulation, resulting in decreasing cleaved-caspase-1 expression. Most important, NLRP3 and GSDMD is significantly increased in gastric tissue of patients with H. pylori infection. Conclusion These findings revealed a critical role of Rabeprazole in cell pyroptosis in patients with H. pylori infection, suggesting that targeting cell pyroptosis is an alternative strategy in improving H. pylori treatment.

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In vitro differentiation of Runx3−/–p53−/–gastric epithelial cells into intestinal type cells

Cancer Science ◽

10.1111/j.1349-7006.2008.00732.x ◽

2008 ◽

Vol 99 (4) ◽

pp. 671-676 ◽

Cited By ~ 12

Author(s):

Hiroshi Fukamachi ◽

Ayako Mimata ◽

Issei Tanaka ◽

Kosei Ito ◽

Yoshiaki Ito ◽

...

Keyword(s):

Epithelial Cells ◽

Intestinal Type ◽

Gastric Epithelial Cells ◽

In Vitro Differentiation

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Helicobacter pylori Induces Gastric Epithelial Cell Apoptosis in Association with Increased Fas Receptor Expression

Infection and Immunity ◽

10.1128/iai.67.8.4237-4242.1999 ◽

1999 ◽

Vol 67 (8) ◽

pp. 4237-4242 ◽

Cited By ~ 106

Author(s):

Nicola L. Jones ◽

Andrew S. Day ◽

Hilary A. Jennings ◽

Philip M. Sherman

Keyword(s):

Cell Death ◽

Helicobacter Pylori ◽

Epithelial Cells ◽

Receptor Expression ◽

Gastric Epithelial Cells ◽

Ags Cells ◽

Fas Receptor ◽

Epithelial Cell Apoptosis ◽

H Pylori

ABSTRACT The mechanisms involved in mediating the enhanced gastric epithelial cell apoptosis observed during infection withHelicobacter pylori in vivo are unknown. To determine whether H. pylori directly induces apoptosis of gastric epithelial cells in vitro and to define the role of the Fas-Fas ligand signal transduction cascade, human gastric epithelial cells were infected with H. pylori for up to 72 h under microaerophilic conditions. As assessed by both transmission electron microscopy and fluorescence microscopy, incubation with acagA-positive, cagE-positive, VacA-positive clinical H. pylori isolate stimulated an increase in apoptosis compared to the apoptosis of untreated AGS cells (16.0% ± 2.8% versus 5.9% ± 1.4%, P < 0.05) after 72 h. In contrast, apoptosis was not detected following infection withcagA-negative, cagE-negative, VacA-negative clinical isolates or a Campylobacter jejuni strain. In addition to stimulating apoptosis, infection with H. pylorienhanced Fas receptor expression in AGS cells to a degree comparable to that of treatment with a positive control, gamma interferon (12.5 ng/ml) (148% ± 24% and 167% ± 24% of control, respectively). The enhanced Fas receptor expression was associated with increased sensitivity to Fas-mediated cell death. Ligation of the Fas receptor with an agonistic monoclonal antibody resulted in an increase in apoptosis compared to the apoptosis of cells infected with the bacterium alone (38.5% ± 7.1% versus 16.0% ± 2.8%,P < 0.05). Incubation with neutralizing anti-Fas antibody did not prevent apoptosis of H. pylori-infected cells. Taken together, these findings demonstrate that the gastric pathogen H. pylori stimulates apoptosis of gastric epithelial cells in vitro in association with the enhanced expression of the Fas receptor. These data indicate a role for Fas-mediated signaling in the programmed cell death that occurs in response toH. pylori infection.

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Overexpression of Bmi-1 Induces the Malignant Transformation of Gastric Epithelial Cells In Vitro

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics ◽

10.3727/096504013x13786659070316 ◽

2013 ◽

Vol 21 (1) ◽

pp. 33-41 ◽

Cited By ~ 10

Author(s):

Yinting Chen ◽

Guoda Lian ◽

Qiubo Zhang ◽

Linjuan Zeng ◽

Chenchen Qian ◽

...

Keyword(s):

Epithelial Cells ◽

Malignant Transformation ◽

Gastric Epithelial Cells

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Roles of SP-A, SP-B, and SP-C in modulation of lipid uptake by pulmonary epithelial cells in vitro

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1996.270.1.l69 ◽

1996 ◽

Vol 270 (1) ◽

pp. L69-L79 ◽

Cited By ~ 10

Author(s):

A. D. Horowitz ◽

B. Moussavian ◽

J. A. Whitsett

Keyword(s):

Epithelial Cells ◽

Lipid Vesicles ◽

Type Ii Cells ◽

Type Ii ◽

Lipid Uptake ◽

3T3 Cells ◽

Alveolar Cell ◽

Pulmonary Epithelial Cells ◽

Nih 3T3

The effects of the surfactant proteins (SP)-A, SP-B, and SP-C on binding and endocytosis of fluorescently labeled lipid vesicles were studied in rat type II epithelial cells and in MLE-12 cells, a pulmonary adenocarcinoma cell line with alveolar cell characteristics. Incorporation of SP-C in lipid vesicles markedly stimulated binding to the cell membrane at 4 degrees C and endocytosis of lipids at 37 degrees C. SP-C enhanced lipid uptake in MLE-12 cells, type II cells, and NIH 3T3 cells. SP-B stimulated lipid uptake in MLE-12 cells, but to a lesser degree. SP-B decreased the amount of lipid uptake stimulated by SP-C, SP-A did not increase endocytosis of lipids by MLE-12 cells or type II cells, but aggregates of lipid were observed associated with the cell surface in the presence of SP-A. Maintenance of active surfactant in the lung may be achieved through the selective uptake and degradation of surfactant subfractions depleted in SP-A and SP-B.

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In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

Applied Sciences ◽

10.3390/app10010325 ◽

2020 ◽

Vol 10 (1) ◽

pp. 325 ◽

Cited By ~ 2

Author(s):

Francisco Javier Rodríguez-Lozano ◽

Sergio López-García ◽

David García-Bernal ◽

Miguel R. Pecci-Lloret ◽

Julia Guerrero-Gironés ◽

...

Keyword(s):

Stem Cells ◽

Cell Adhesion ◽

Cell Viability ◽

Periodontal Ligament ◽

Biological Properties ◽

Periodontal Ligament Stem Cells ◽

Bioactive Materials ◽

Tukey Test ◽

Vitro Effect

New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (p < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (p < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material.

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In Vitro Assessment of the Functional Dynamics of Titanium with Surface Coating of Hydroxyapatite Nanoparticles

Materials ◽

10.3390/ma12050840 ◽

2019 ◽

Vol 12 (5) ◽

pp. 840 ◽

Cited By ~ 8

Author(s):

José Henrique de Lima Cavalcanti ◽

Patrícia Matos ◽

Cresus Vinícius Depes de Gouvêa ◽

Waldimir Carvalho ◽

José Luis Calvo-Guirado ◽

...

Keyword(s):

Cell Adhesion ◽

Adhesion Formation ◽

Titanium Implants ◽

Tissue Response ◽

Mesenchymal Progenitor Cells ◽

Machined Surface ◽

Implant Surface ◽

Sem Images ◽

Vitro Assay

Manipulation of implant surface characteristics constitutes a promising strategy for improving cell growth and tissue response on a variety of materials with different surface topographies. Mesenchymal progenitor cells with a capacity to respond to titanium surface stimuli and differentiate into osteoblasts were used to perform comparative tests between two different implant topographies, including their functional interaction with pre-osteoblasts directly seeded onto the implants. Functional analysis of nanostructured implant surfaces was performed by in vitro assay analysis. The machined surface of titanium implants (mach group) was used as a control and compared with a nanoparticle HA activated surface implant (nano group), developed by the deposition of pure crystalline hydroxyapatite. Cell culture on the nano group surface resulted in higher cell adhesion and cultured osteoblast viability compared with the mach group. Scanning electron microscope (SEM) images revealed a stable interaction, indicated by the presence of focal cell adhesion formation. These results together with positive mineralization assays showed the nano group to be an excellent scaffold for bone-implant integration.

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