The effect of Allium cepa extract on the chitosan/PLGA scaffolds bioactivity

Allium cepa extracts (AC) allow the fabrication of a biomaterial that, together with chitosan and PLGA, could be osteoconductive and promote a better and faster regeneration of bone tissue, with biocompatibility and biomineralization properties. In this work, scaffolds were developed by the thermally induced phase separation (TIPS) technique. An in vitro bioactivity analysis was performed using simulated body fluid (SBF). Scanning electron microscopy (SEM), energy dispersion spectroscopy, and infrared spectroscopy were used for the scaffolds characterization. The results showed a structure with a pore size distribution between 50 and 100 μm, which allowed the uniform formation of biological apatite crystals on the surface of the scaffolds. The chitosan/policaprolactone/ Allium cepa scaffold (ChPAC) showed the most promising results with a ratio of P/Ca between 1.6 and 1.7, a value very close to that of hydroxyapatite.

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In vitro characterization of hierarchical 3D scaffolds produced by combining additive manufacturing and thermally induced phase separation

Journal of Biomaterials Science Polymer Edition ◽

10.1080/09205063.2020.1841535 ◽

2020 ◽

pp. 1-23

Author(s):

Azizeh-Mitra Yousefi ◽

Joseph Powers ◽

Kaylie Sampson ◽

Katherine Wood ◽

Carter Gadola ◽

...

Keyword(s):

Phase Separation ◽

Additive Manufacturing ◽

Thermally Induced Phase Separation ◽

3D Scaffolds ◽

Thermally Induced ◽

In Vitro Characterization

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Improvement of Mechanical Strength of Tissue Engineering Scaffold Due to the Temperature Control of Polymer Blend Solution

Journal of Functional Biomaterials ◽

10.3390/jfb12030047 ◽

2021 ◽

Vol 12 (3) ◽

pp. 47

Author(s):

Azizah Intan Pangesty ◽

Mitsugu Todo

Keyword(s):

Heat Treatment ◽

Phase Separation ◽

Mechanical Strength ◽

Polymer Blend ◽

Temperature Control ◽

Thermally Induced Phase Separation ◽

Thermally Induced ◽

Polymeric Scaffolds ◽

Cell Study

Polymeric scaffolds made of PCL/PLCL (ratio 1:3, respectively) blends have been developed by using the Thermally Induced Phase Separation (TIPS) process. A new additional technique has been introduced in this study by applying pre-heat treatment to the blend solution before the TIPS process. The main objective of this study is to evaluate the influence of the pre-heat treatment on mechanical properties. The mechanical evaluation showed that the mechanical strength of the scaffolds (including tensile strength, elastic modulus, and strain) improved as the temperature of the polymer blend solution increased. The effects on the microstructure features were also observed, such as increasing strut size and differences in phase separation morphology. Those microstructure changes due to temperature control contributed to the increasing of mechanical strength. The in vitro cell study showed that the PCL/PLCL blend scaffold exhibited better cytocompatibility than the neat PCL scaffold, indicated by a higher proliferation at 4 and 7 days in culture. This study highlighted that the improvement of the mechanical strength of polymer blends scaffolds can be achieved using a very versatile way by controlling the temperature of the polymer blend solution before the TIPS process.

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Advance on Resveratrol Application in Bone Regeneration: Progress and Perspectives for Use in Oral and Maxillofacial Surgery

Biomolecules ◽

10.3390/biom9030094 ◽

2019 ◽

Vol 9 (3) ◽

pp. 94 ◽

Cited By ~ 13

Author(s):

Denise Murgia ◽

Rodolfo Mauceri ◽

Giuseppina Campisi ◽

Viviana De Caro

Keyword(s):

Biological Effects ◽

Maxillofacial Surgery ◽

Oral And Maxillofacial Surgery ◽

Positive Effects ◽

Natural Polyphenol ◽

Regeneration Of Bone Tissue ◽

Experimental Findings ◽

Regeneration Of Bone

The natural polyphenol Resveratrol (RSV) claims numerous positive effects on health due to the well documented biological effects demonstrating its potential as a disease-preventing agent and as adjuvant for treatment of a wide variety of chronic diseases. Since several studies, both in vitro and in vivo, have highlighted the protective bone aptitude of RSV both as promoter of osteoblasts’ proliferation and antagonist of osteoclasts’ differentiation, they could be interesting in view of applications in the field of dentistry and maxillofacial surgery. This review has brought together experimental findings on the use of RSV in the regeneration of bone tissue comprising also its application associated with scaffolds and non-transfusional hemocomponents.

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Assessment of Antimicrobial Activity of Onion Extract (Allium cepa) on Staphylococcus aureus; in vitro study

International Journal of Advances in Chemical Engineering and Biological Sciences ◽

10.15242/ijacebs.c1213068 ◽

2014 ◽

Vol 1 (1) ◽

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Allium Cepa ◽

In Vitro Study ◽

Vitro Study ◽

Onion Extract

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Regeneration of bone tissue under implantation of sapphire with titanium coating

ORTHOPAEDICS TRAUMATOLOGY and PROSTHETICS ◽

10.15674/0030-59872014324-29 ◽

2014 ◽

Vol 0 (3) ◽

pp. 24 ◽

Cited By ~ 1

Author(s):

Volodymyr Filipenko ◽

Inna Batura ◽

Anatoliy Andreyev ◽

Оleksey Marushchak

Keyword(s):

Bone Tissue ◽

Titanium Coating ◽

Regeneration Of Bone Tissue ◽

Regeneration Of Bone

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Temulawak (Curcuma xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v40i2.6383 ◽

1970 ◽

Vol 40 (2) ◽

Author(s):

Waras Nurcholis ◽

Edy Djauhari Purwakusumah ◽

Mono Rahardjo ◽

Latifah K. Darusman

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Brine Shrimp ◽

Clay Soil ◽

Herbal Medicines ◽

Environmental Condition ◽

In Vitro Bioactivity ◽

The Family ◽

Promising Line

Temulawak (Curcuma xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol

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Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water

Waste and Biomass Valorization ◽

10.1007/s12649-021-01477-z ◽

2021 ◽

Author(s):

Jaroslava Švarc-Gajić ◽

Víctor Cerdà ◽

Cristina Delerue-Matos ◽

Pavle Mašković ◽

Sabrina Clavijo ◽

...

Keyword(s):

Subcritical Water ◽

Chemical Characterization ◽

In Vitro Bioactivity

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Characterization of the SARS-CoV-2 coronavirus X4-like accessory protein

Egyptian Journal of Medical Human Genetics ◽

10.1186/s43042-021-00160-1 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Olanrewaju Ayodeji Durojaye ◽

Nkwachukwu Oziamara Okoro ◽

Arome Solomon Odiba

Keyword(s):

Rapid Development ◽

Protein A ◽

The Novel ◽

Quality Model ◽

A Value ◽

Model Protein ◽

Novel Coronavirus ◽

Global Threat

Abstract Background The novel coronavirus SARS-CoV-2 is currently a global threat to health and economies. Therapeutics and vaccines are in rapid development; however, none of these therapeutics are considered as absolute cure, and the potential to mutate makes it necessary to find therapeutics that target a highly conserved regions of the viral structure. Results In this study, we characterized an essential but poorly understood coronavirus accessory X4 protein, a core and stable component of the SARS-CoV family. Sequence analysis shows a conserved ~ 90% identity between the SARS-CoV-2 and previously characterized X4 protein in the database. QMEAN Z score of the model protein shows a value of around 0.5, within the acceptable range 0–1. A MolProbity score of 2.96 was obtained for the model protein and indicates a good quality model. The model has Ramachandran values of φ = − 57o and ψ = − 47o for α-helices and values of φ = − 130o and ψ = + 140o for twisted sheets. Conclusions The protein data obtained from this study provides robust information for further in vitro and in vivo experiment, targeted at devising therapeutics against the virus. Phylogenetic analysis further supports previous evidence that the SARS-CoV-2 is positioned with the SL-CoVZC45, BtRs-BetaCoV/YN2018B and the RS4231 Bat SARS-like corona viruses.

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