scholarly journals Simultaneous Feulgen densitometry and autoradiographic grain counting with the Quantimet 720D image-analysis system. I. Estimation of nuclear DNA content in 3H-thymidine-labeled cells.

1982 ◽  
Vol 30 (1) ◽  
pp. 35-48 ◽  
Author(s):  
R J Sklarew
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Image Analysis System ◽  
Feulgen Densitometry ◽  
Analysis System

scholarly journals An image analysis system for cervical cytology automation using nuclear DNA content.

1979 ◽  
Vol 27 (1) ◽  
pp. 613-620 ◽  
Author(s):  
J H Tucker
Keyword(s):  
Image Analysis ◽  
Measurement Accuracy ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Cervical Cytology ◽  
Image Analysis System ◽  
Cell Nuclei ◽  
Computer Image Analysis ◽  
Integrated Optical ◽  
Analysis System

An experimental computer/image analysis system has been used to investigate cytology automation techniques based on nuclear DNA measurement and morphological artefact rejector tests. The system automatically measures and normalizes the integrated optical density of cell nuclei in specially prepared cervical cytology specimens, and selects any objects with abnormally high values for further analysis. These are then analyzed by morphological and densitometric tests designed to eliminate false positive signals caused by non-nuclear artefacts. The coordinates of the remaining abnormal nuclei are recorded so that they can subsequently be relocated and examined by a cytotechnician. Preliminary results are given showing the measurement accuracy of the system and the performance of the artefact rejection tests.

scholarly journals Simultaneous Feulgen densitometry and autoradiographic grain counting with the Quantimet 720D image-analysis system. III. Improvements in Feulgen densitometry.

1983 ◽  
Vol 31 (10) ◽  
pp. 1224-1232 ◽  
Author(s):  
R J Sklarew
Keyword(s):  
Image Analysis ◽  
Imaging System ◽  
Rat Kidney ◽  
Light Sources ◽  
Kidney Cells ◽  
Image Analysis System ◽  
Replication Error ◽  
Feulgen Densitometry ◽  
Optical Configuration ◽  
Analysis System

A method has been developed for densitometric estimation of the Feulgen-stained DNA content of 3H-labeled nuclei in autoradiographs in conjunction with automated grain counting using a Quantimet Imaging System. Refinements in the methodology are reported which include 1) the incorporation of an Image-Editor Module into the Quantimet module configuration; 2) the optimization of incident illumination based upon evaluation of various light sources; 3) changes in the optical configuration which reduce glare and minimize the level of monitor shading correction; 4) the optimization of scanner sensitivity; and 5) the evaluation of cell-flattening and staining with respect to densitometry resolution and sensitivity. These refinements resulted in a CV of less than 6.4% in the G-1 and G-2 DNA peaks of rat kidney cells in autoradiographs compared to the previous CV of 10.5%, and a G-2 to G-1 ratio of 2.025. For a fixed field position the CV was 5.1% and the replication error less than 1.0%.

Central values and variation of measured nuclear DNA content in imprints of normal tissues determined by image analysis

Cytometry ◽  
1989 ◽  
Vol 10 (4) ◽  
pp. 382-387 ◽  
Author(s):  
Suzanne R. Taylor ◽  
Linda Titus-Ernstoff ◽  
Suzette Stitely
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Central Values ◽  
Normal Tissues

Evaluation of a simple method for measuring the cellular DNA content of mouse oocytes and embryos, human fibroblasts and parthenogenetically activated human oocytes using a computerised image analysis system(Seescan)

Zygote ◽  
1995 ◽  
Vol 3 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Alison S. Taylor ◽  
Peter R. Braude
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Lung Fibroblasts ◽  
Short Exposure ◽  
Image Analysis System ◽  
Simple Method ◽  
Cytoplasmic Staining ◽  
Human Oocytes ◽  
Computerised Image Analysis ◽  
Analysis System

SummaryWe report the use of a simple, reproducible, photocytometric method for measuring nuclear DNA content of DAPI-stained cells, using a computerised image analysis system: Seescan. As this technique is non-destructive and uses very short exposure to ultraviolet light, it can be used for either fixed or vital material. After correcting for any background cytoplasmic staining, the intensity of nuclear stain was measured by the Seescan and compared with that of control cells of known ploidy. Fixed material was found to stain more intensely than live material initially, but demonstrated a rapid loss of nuclear intensity over the first 90 min following removal from DAPI, after which the level plateaued. In contrast, live cells showed no change in nuclear intensity with time. The system was validated by measuring the DNA content of carefully timed mouse blastomeres, human fetal lung fibroblasts and parthenogenetically activated human oocytes. The results obtained were appropriate for the developmental stage or phenotypic appearance of each of the cell types measured.

scholarly journals IMAGE ANALYSIS OF CHROMATIN IN CELLS OF PREIMPLANTATION MOUSE EMBRYOS

1974 ◽  
Vol 63 (1) ◽  
pp. 227-233 ◽  
Author(s):  
Wojciech Sawicki ◽  
Jan Rowínski ◽  
Jan Abramczuk
Keyword(s):  
Cell Cycle ◽  
Image Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Mouse Embryos ◽  
Stage Of Development ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
The Mean

Mouse two-celled embryos and blastulae were Feulgen stained and the DNA content of their nuclei was measured with an integrating microdensitometer. The cells considered on the basis of their nuclear DNA content to be in G1, S, and G2 phases of the cell cycle were selected and their total chromatin area and chromatin areas at different gray levels were measured by the image analyzing computer, Quantimet. The measurements were aimed at quantitation of several features of the chromatin morphology of cells in different functional states. The total area of chromatin was found to increase, and the mean density of chromatin to decrease, from the G1 to the G2 phase of the cell cycle in both two-celled embryos and blastulae. The area of chromatin decreased, and the mean density of chromatin increased, as embryos developed from two-celled to blastula stage. It was concluded that nuclear morphology in preimplantation mouse embryos depends on both the phase of the cell cycle and the stage of development. The method of image analysis described was found to be useful for quantitation of changes in chromatin morphology.

scholarly journals Detection of suspicious cells and rejection of artefacts in cervical cytology using the Leyden Television Analysis System.

1979 ◽  
Vol 27 (1) ◽  
pp. 629-634 ◽  
Author(s):  
I Al ◽  
J S Ploem
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Detection Method ◽  
Nuclear Dna Content ◽  
Cervical Cytology ◽  
Detection Methods ◽  
Preliminary Results ◽  
Chromatin Pattern ◽  
Analysis System ◽  
Blind Trials

In slide based automation of cervical cytology the first stage of analysis involves finding possibly suspicious cells, or areas on the slide with these types of cells. By using a television based system such as the Leyden Television Analysis System (LEYTAS), a number of detection methods can be applied to rapidly screen a large number of fields automatically for suspicious cells. In this paper, results using a parameter based on increased nuclear DNA content of cells are given and a second detection method based on a chromatin pattern feature, called chromatin contrast, is discussed. Two blind trials on 41 positive and 22 negative cervical slides, using the Leyden Television Analysis System to detect suspicious cells with an increased nuclear DNA content, were promising. In 1 of the 41 positive cases no suspicious cells were found. In the negative specimens, suspicious cells were detected in 1 of 9 cases and 1 of 13 cases, with the two detection parameters investigated. These findings are discussed and some automatic artefact rejection procedures with preliminary results are given.

Chemical Classification and Particle Sizing of Foundry Sands

1985 ◽  
Vol 43 ◽  
pp. 388-389
Author(s):  
D.S. DeMiglio
Keyword(s):  
Image Analysis ◽  
Energy Dispersive Spectrometer ◽  
Image Analysis System ◽  
Representative Sampling ◽  
Foundry Sands ◽  
Large Atomic Number ◽  
Backscattered Electron ◽  
Sand Particles ◽  
Analysis System ◽  
Reclamation System

Much progress has been made in recent years towards the development of closed-loop foundry sand reclamation systems. However, virtually all work to date has determined the effectiveness of these systems to remove surface clay and metal oxide scales by a qualitative inspection of a representative sampling of sand particles. In this investigation, particles from a series of foundry sands were sized and chemically classified by a Lemont image analysis system (which was interfaced with an SEM and an X-ray energy dispersive spectrometer) in order to statistically document the effectiveness of a reclamation system developed by The Pangborn Company - a subsidiary of SOHIO.The following samples were submitted: unreclaimed sand; calcined sand; calcined & mechanically scrubbed sand and unused sand. Prior to analysis, each sample was sprinkled onto a carbon mount and coated with an evaporated film of carbon. A backscattered electron photomicrograph of a field of scale-covered particles is shown in Figure 1. Due to a large atomic number difference between sand particles and the carbon mount, the backscattered electron signal was used for image analysis since it had a uniform contrast over the shape of each particle.

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