Burr Red Blood Cells (BC) In Patients with Disseminated Intravascular Coagulation (DIC)

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 5141-5141
Author(s):  
Vipul R. Patel ◽  
Jatinder Khokhar ◽  
Hemant S Murthy ◽  
Albert S. Braverman
Keyword(s):  
Renal Insufficiency ◽  
Red Blood Cells ◽  
Disseminated Intravascular Coagulation ◽  
Blood Cells ◽  
Conflicts Of Interest ◽  
Hypertonic Medium ◽  
Intravascular Coagulation ◽  
Acute Renal Insufficiency ◽  
Hb S ◽  
The Mean

Abstract Abstract 5141 Burr red blood cells (BC) in patients with disseminated intravascular coagulation (DIC). Background: Angiopathic hemolysis with schistocytes occurs in a minority of DIC patients. Burr cells (BC), are morphologically homogeneous, with a serrated membrane resembling that of red cells (RBC) in a hypertonic medium. They are associated with acute renal insufficiency, and have been observed in DIC. Methods: Criteria for patient inclusion were sepsis, often with multi-organ failure and hypotension. Of the 39 patients studied, 20 had DIC, based on platelet counts <100,000, and an International Society of Hemostasis and Thrombosis score of ≥5, derived from D-dimer or fibrin split product levels, prothrombin time elevations, and fibrinogen levels. The percentages of BC and schistocytes were determined by 1000 RBC counts, without knowledge of data concerning DIC. Patients' [Hb]'s, serum creatinine and blood urea nitrogen (BUN), were recorded. Results: Five of the 20 DIC patients succumbed to their acute illness, while 15 recovered without developing renal insufficiency. The BC%'s of the 20 DIC patients ranged from 0–80%, with a median of 18% and a mean of 27%; in the 19 patients without DIC the range was 0–45%, the median 6.4% and the mean 11% (p=0.046). The schistocyte percentages in patients with and without DIC ranged from 0.20–17, and 0–78; the respective medians and means were 1.1% and 1.0%, and 3.0–5.8%. The median and mean [Hb]'s of the patients with and without DIC were 9.3 and 11, and 9.6 and 10.3, respectively. BC% did not correlate with the [Hb]. The median creatinine levels of patients with and without DIC were 0.92 and 0.93 mg/dl, though the DIC patients' BUN's were higher (medians 31 vs 15). The BC% correlated with neither the BUN nor the BUN/creatinine ratio. The median and mean serum sodium levels in patients with and without DIC were 137. Conclusion: BC's are significantly more frequent in septic patients with DIC than in those without it. BC% did not correlate with anemia, implying that BC do not cause significant hemolysis, though there was a trend to lower [Hb] in the DIC patients. BC% was much higher than that of schistocytes in both groups, and schistocyte percentages did not correlate with DIC or BC%. Because of the DIC patients' higher BUN's and BUN/creatinine ratio's, we cannot exclude a role for the factors, which caused pre-renal azotemia in BC formation. BC formation may prove to be a criterion for DIC. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Pathogenetic therapy of vascular-platelet hemostasis disorders in canine acute spontaneous babesiosis

2021 ◽  
Vol 23 (103) ◽  
pp. 96-102
Author(s):  
O. A. Dubova ◽  
A. O. Rudchenko ◽  
D. V. Feshchenko ◽  
A. A. Dubovyi ◽  
I. V. Chala ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Red Blood Cells ◽  
Disseminated Intravascular Coagulation ◽  
Blood Cells ◽  
Vascular Wall ◽  
Crystalloid Solution ◽  
Intravascular Coagulation ◽  
Plasma Substitute ◽  
Pathogenetic Therapy ◽  
Significant Deficit

The article presents the results of a study of the vascular-platelet hemostasis disorders processes in complications of canine acute spontaneous babesiosis, as well as a clinical trial of plasma substitute infusion for the purpose of identified disorders pathogenetic therapy. It was found that acute spontaneous Babesiosis is accompanied by complications in the form of subcompensated shock and a thrombogenic link of disseminated intravascular coagulation syndrome (DIC). This determines the potential risk of complications with a cautious prognosis. The basis for the diagnosis of complications is the establishment of the following changes: a significant deficit in the volume of circulating blood (a decrease in the hematocrit value, the volume of circulating plasma, the volume of circulating red blood cells, the specific volume of circulating blood), as well as significant changes in the functioning of the vascular-platelet link of hemostasis – thrombocytopenia against the background of an increase in the spontaneous aggregation ability of platelets and red blood cells, an increase in the wetting index of the vascular wall, which determines the thrombogenic state, and pronounced thrombocytopenia indicates the consumption of these shaped elements in blood clots. The described changes indicate the development of subcompensated shock and the thrombogenic component of DIC syndrome. Given the prognostic danger of established complications, there is a need for pathogenetic therapy of severe conditions. Infusions of plasma substitute solutions have been proposed to eliminate shock phenomena and the thrombogenic state of disseminated intravascular coagulation syndrome. A clinical trial of intravenous administration of Rheosorbylact solution and a mixture of Rheosorbylact with Dipyridamole was conducted in a comparative aspect. It is shown that a mixture of Rheosorbylact 100 ml and Dipyridamole solution 0.5 % 4 ml in the form of infusions at a dose of 5 ml/kg of animal body weight per day for 3 days can bring hemodynamic parameters and parameters of vascular-platelet hemostasis to physiological ones within 48 hours compared to an infusion of Rheosorbylact solution in its pure form. The synergy of the crystalloid solution of Rheosorbylact and the disaggregating vasodilator Dipyridamole enhances the disaggregating effect of both drugs, and the crystalloid solution itself is able to restore the lost volume of circulating blood.

Disseminated intravascular coagulation syndrome

2020 ◽  
pp. 22-40
Author(s):  
A. Kulikov
Keyword(s):  
Clinical Practice ◽  
Disseminated Intravascular Coagulation ◽  
Blood Cells ◽  
Physical State ◽  
Clinical Manifestations ◽  
Vascular Wall ◽  
Stages Of Development ◽  
Intravascular Coagulation ◽  
Hemostatic Disorder

Presented material reveals main links in the pathogenesis of hemostatic disorder. In particular, attention is paid to the role of the lungs, liver and other organs in the development of this process. Role of vascular wall and blood cells in regulation of the physical state of blood is described in detail. The most frequent factors leading to hypercoagulation are indicated. Difference between hypercoagulation and thrombophilia is shown. The latter is found in clinical practice quite often, but at the same time, it is poorly diagnosed. Such a terrible complication of hemostatic disorder as disseminated intravascular coagulation is described. Its classification, stages of development, clinical manifestations are offered to the readers.

A Study of the Metabolism of Fibrinogen after Surgical Operations

1971 ◽  
Vol 41 (2) ◽  
pp. 141-152 ◽  
Author(s):  
J. A. Hickman
Keyword(s):  
Venous Thrombosis ◽  
Disseminated Intravascular Coagulation ◽  
Half Life ◽  
Plasma Fibrinogen ◽  
Intravascular Coagulation ◽  
Plasma Loss ◽  
The Mean ◽  
Chronic Disorders ◽  
Contrast Group ◽  
Surgical Operations

1. The turnover of fibrinogen was studied in twenty-eight patients after moderate and major surgical operations and in a contrast group of thirteen patients suffering from miscellaneous chronic disorders. 2. After operation the mean biological half-life of fibrinogen was 60.1 h (SD 16.4) and the mean plasma loss of fibrinogen was 40.1% (SD 12.6) of the intravascular fibrinogen pool each 24 h. In the contrast group, respective values of 96.2 h (SD 13.1) and 22.5% (SD 2.0) were obtained, which are in agreement with published values for the normal catabolism of fibrinogen. 3. When the post-operative increase in the plasma fibrinogen pool was taken into account, a massive increase in the absolute plasma loss of fibrinogen was observed with values approaching those reported for albumin turnover. 4. Possible sites of loss and the relevance of this investigation to the detection of disseminated intravascular coagulation and venous thrombosis are discussed.

Measuring tissue factor (factor III) activity in plasma.

1989 ◽  
Vol 35 (9) ◽  
pp. 1897-1900 ◽  
Author(s):  
C Fukuda ◽  
K Iijima ◽  
K Nakamura
Keyword(s):  
Tissue Factor ◽  
Disseminated Intravascular Coagulation ◽  
Coagulation Factors ◽  
High Plasma ◽  
Mean Value ◽  
Chromogenic Substrate ◽  
Intravascular Coagulation ◽  
Endogenous Inhibitors ◽  
Tissue Thromboplastin ◽  
The Mean

Abstract This is a method for measuring tissue factor (TF, Factor III, tissue thromboplastin) activity in plasma by using a chromogenic substrate. As pretreatment, the euglobulin fraction of plasma was prepared by removing endogenous inhibitors and heated at 60 degrees C for 3 min to remove fibrinogen. This allowed us to measure the low TF activity in plasma that could not otherwise be measured. Neither phospholipids nor coagulation factors VII, IX, X, or Xa in the samples interfere. Within-run and day-to-day reproducibility were both good. The mean value obtained by this method for normal persons was 1.02 (SD 0.91) arbitrary units/L. A markedly high plasma TF activity of 20 arb. units/L or more was observed in patients with some types of disseminated intravascular coagulation.

scholarly journals The Quality Assessment of Stored Red Blood Cells Probed Using Atomic-Force Microscopy

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
I. M. Lamzin ◽  
R. M. Khayrullin
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Characteristics ◽  
High Increase ◽  
Mean Values ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
The Moment

At the moment the suitability of stored red blood cells (sRBC) for transfusion is checked by routine methods such as haemoglobin estimation and the level of haemolysis. These methods cannot characterize directly the quality of the membranes of sRBC. The aim of this work is to assess the quality of sRBC based on such criteria as the membrane’s stiffness and the size and the form of sRBC. Materials and Methods. We have investigated 5 series of dry cytosmears of the sRBC which had been kept in blood bank in a period from 1 to 35 days. After AFM imaging, in every specimen, 5 RBC were chosen at random; the diameter, the height, and the stiffness were measured on each of them. Results. The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter. Conclusion. Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC. This parameter along with the morphological characteristics can be used as criterion for assessment of applicability of the sRBC for blood transfusion.

Erythroplateletapheresis: A New Strategy in the Global Apheresis Program

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 17-19
Author(s):  
G. Menichella ◽  
M. Ciarli ◽  
R. Serafini ◽  
L. Pierelli ◽  
M. Vittori ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Plasma Volume ◽  
Blood Cells ◽  
Storage Time ◽  
Blood Donation ◽  
Mean Values ◽  
Packed Red Blood Cells ◽  
New Strategy ◽  
The Mean

Blood donation allowed by cell separators can offer higher performance and higher yield to guarantee better quality and pureness of collected products. New systems for the collection of platelet concentrate (PC) and packed red blood cells (PRBC) are currently available. The aim of our work was to test the possibility of preparing PC routinely from normal apheresis donors in a minimum amount of time while providing a second product. Over a 3-month period we performed 40 procedures using the Hemonetics MCS3P blood cell separator and the Dideco Excel. The mean values of platelet yield were 2.8 x 1011 (range 1.4-4.1) with the MCS3P and 3.49 x 1011 (range 2.9-3.9) with the Excel, in a plasma volume of 240 ml and 215 ml respectively; the PRBC units were added with SAG-Mannitol allowing a storage time of 42 days. Collection times were 71’ and 48’ respectively. Donor tolerance was analogous to phateletapheresis or plasmapheresis.

scholarly journals The Nature of the Hemorrhagic Disorder Accompanying Hemolytic Transfusion Reactions in Man

Blood ◽  
1957 ◽  
Vol 12 (9) ◽  
pp. 834-843 ◽  
Author(s):  
JULIUS R. KREVANS ◽  
DUDLEY P. JACKSON ◽  
C. LOCKARD CONLEY ◽  
ROBERT C. HARTMANN
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Fibrinolytic Activity ◽  
Hemorrhagic Diathesis ◽  
Intravascular Coagulation ◽  
Transfusion Reactions

Abstract A hemorrhagic diathesis has been observed in 2 patients who received 500 ml. of incompatible whole blood. In both, hypofibrinogenemia, hypoprothrombinemia and thrombocytopenia were observed and there was no evidence of increased fibrinolytic activity. In one, accelerin activity was reduced and there was transient evidence of a low-titered circulating anticoagulant. The most likely explanation for the observed changes is intravascular coagulation in the recipient, presumably initiated by the thromboplastin-like activity of the hemolyzed red blood cells.

Catechol O-Methyltransferase in Red Blood Cells of Schizophrenic, Depressed, and Normal Human Subjects

1976 ◽  
Vol 128 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Helen L. White ◽  
Malcolm N. McLeod ◽  
Jonathan R. T. Davidson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Normal Subjects ◽  
Enzyme Preparations ◽  
Human Red Blood Cells ◽  
Schizophrenic Patients ◽  
Normal Human ◽  
The Mean ◽  
Isoelectric Ph

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

A survey of the potassium concentration in the red blood cells of British breeds of sheep

1957 ◽  
Vol 48 (4) ◽  
pp. 433-437 ◽  
Author(s):  
John V. Evans ◽  
M. S. Mounib
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Potassium Concentration ◽  
High Potassium ◽  
The Mean ◽  
High Level ◽  
Mean Concentration ◽  
Representative Samples

The concentrations of potassium in the whole blood of representative samples of sixteen British breeds of sheep have been studied.The proportion of sheep with a high level of potassium in the whole blood (high potassium or HK type) was found to differ significantly between breeds. It ranged from 0% in the English Leicester to 73% in the Rough Fell.There were significant differences between breeds in the mean concentration of potassium in the whole blood of both the LK and HK sheep.

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