Chemical Characterization of Three Hemoglobins G

Abstract Three abnormal hemoglobins identified as G by their electrophoretic mobility and performance on resin chromatography have been discovered in three healthy unrelated Negro individuals in Texas. These three G hemoglobins have been shown to belong to the same molecular species and to have a substitution in the 43rd residue of the β chain. Glutamic acid, which is present in this position in hemoglobin A, has been replaced by alanine in hemoglobin G. According to the recent suggestions on nomenclature of abnormal hemoglobins, hemoglobin GGalveston may be formulated as α24β243 Glu→ ala. Moreover, it has been shown that the following hemoglobins belong to the same molecular species: hemoglobin GGalveston, hemoglobin GPort Arthur, and hemoglobin GTexas. This alteration is the first to be described in the fifth tryptic peptide of the β chain of human hemoglobin and differs from all abnormal hemoglobins previously reported.

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Solution1H NMR Characterization of Axial Interactions of the Proximal and Distal His in the Cyanomet Complexes of the Isolated Chains and the 65 kDa Intact Tetramer of Human Hemoglobin A

Journal of the American Chemical Society ◽

10.1021/ja004168w ◽

2001 ◽

Vol 123 (18) ◽

pp. 4266-4274 ◽

Cited By ~ 6

Author(s):

Gerd N. La Mar ◽

Urszula Kolczak ◽

Anh-Tuyet T. Tran ◽

Ellen Y. T. Chien

Keyword(s):

Human Hemoglobin ◽

Hemoglobin A ◽

Nmr Characterization

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Chemical characterization of ‘Pecorino Di Farindola’ cheese during ripening

Italian Journal of Food Science ◽

10.15586/ijfs.v33i1.1964 ◽

2021 ◽

Vol 33 (1) ◽

pp. 46-51

Author(s):

Serena Niro ◽

Alessandra Fratianni ◽

Annacristina D'Agostino ◽

Ivan Notardonato ◽

Gianfranco Panfili

Keyword(s):

Amino Acids ◽

Glutamic Acid ◽

Free Amino Acids ◽

Free Amino ◽

Chemical Characterization ◽

Ripening Stages

This study evaluated the nutritional and sensorial characteristics of Pecorino di Farindola cheese at different commercial ripening stages. Moreover, in order to assess effectively the peculiar features of this product, the evolution of proteolysis and lipolysis, together with that of free amino acids (FAAs), was studied throughout ripening. A marked proteolysis of Pecorino di Farindola was found. At the end of ripening, FAAs with the highest content were glutamic acid, valine, leucine and lysine. Long-ripened cheeses had a light spicy feature that distinguishes them from other Italian Pecorino cheeses.

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Galactomannoproteins of Aspergillus fumigatus

Eukaryotic Cell ◽

10.1128/ec.4.7.1308-1316.2005 ◽

2005 ◽

Vol 4 (7) ◽

pp. 1308-1316 ◽

Cited By ~ 77

Author(s):

W. Morelle ◽

M. Bernard ◽

J.-P. Debeaupuis ◽

M. Buitrago ◽

M. Tabouret ◽

...

Keyword(s):

Monoclonal Antibody ◽

Aspergillus Fumigatus ◽

Phospholipase C ◽

Molecular Species ◽

Infected Patient ◽

Fungal Pathogens ◽

Chemical Characterization ◽

Major Molecular Species

ABSTRACT Galactofuranose-containing molecules have been repeatedly shown to be important antigens among human fungal pathogens, including Aspergillus fumigatus. Immunogenic galactofuran determinants have been poorly characterized chemically, however. We reported here the characterization of two glycoproteins of A. fumigatus with an N-glycan containing galactofuranose. These proteins are a phospholipase C and a phytase. Chemical characterization of the N-glycan indicates that it is a mixture of Hex5-13HexNAc2 oligosaccharides, the major molecular species corresponding to Hex6-8HexNAc2. The N-glycan contained one galactofuranose unit that was in a terminal nonreducing position attached to the 2 position of Man. This single terminal nonreducing galactofuranose is essential for the immunoreactivity of the N-glycans assessed either with a monoclonal antibody that recognizes a tetra-β-1,5-galactofuran chain of galactomannan or with Aspergillus-infected patient sera.

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Mechanical-Chemical Characterization of the Effects of Type, Dosage, and Treatment Methods of Rejuvenators in Aged Bituminous Materials

Transportation Research Record Journal of the Transportation Research Board ◽

10.1177/0361198120909110 ◽

2020 ◽

Vol 2674 (3) ◽

pp. 126-138 ◽

Cited By ~ 1

Author(s):

Gabriel Nsengiyumva ◽

Hamzeh F. Haghshenas ◽

Yong-Rak Kim ◽

Santosh Reddy Kommidi

Keyword(s):

Chemical Characterization ◽

Flow Number ◽

Recycled Asphalt ◽

Test Analysis ◽

Treatment Methods ◽

Bituminous Materials ◽

And Performance ◽

Performance Grading ◽

Binder Level

Proper use of rejuvenators can improve the properties and performance of aged asphalt mixtures; however, there is currently a lack of understanding on how rejuvenator treatment details, such as type-dosage of rejuvenators and blending-curing of rejuvenation, affect properties and performance characteristics. This study aims to investigate the effects of type, dosage, and treatment methods of rejuvenators when added into aged bituminous materials. To meet the goal, three rejuvenators, categorized by their production technology, were selected to improve a high-recycled asphalt pavement mixture by conducting various binder-level and mixture-level tests. For the binder-level testing, the performance grading (PG) method was used to primarily determine proper dosages targeting desired binder grades, and two chemical tests (Fourier-transform infrared spectroscopy and Saturate, Aromatic, Resin and Asphaltene) were conducted to examine chemical characteristics altered by rejuvenation and the further aging process. The selected dosage levels from the binder testing were then applied to mixture-level performance evaluation by conducting two tests: flow number for rutting and semicircular bending fracture for cracking. Test-analysis results indicated that although it can successfully determine the proper dosage range of rejuvenators, PG binder testing is limited to only assessing the effects of rejuvenators on mechanical properties; this can be better aided by integrating chemical characterization that provides a more in-depth material-specific rejuvenation process. Moreover, it appears that rejuvenation methods can alter the performance of aged mixtures. Therefore, the selection of rejuvenators and their implementation into practice should be carried out considering multiple aspects, not only its PG recovery.

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Preparation and Kinetic Characterization of a Series of βW37 Variants of Human Hemoglobin A: Evidence for High-Affinity T Quaternary Structures†

Biochemistry ◽

10.1021/bi970866q ◽

1998 ◽

Vol 37 (13) ◽

pp. 4325-4335 ◽

Cited By ~ 13

Author(s):

Laura D. Kwiatkowski ◽

Hilda L. Hui ◽

Anita Wierzba ◽

Robert W. Noble ◽

Roxanne Y. Walder ◽

...

Keyword(s):

Human Hemoglobin ◽

Kinetic Characterization ◽

High Affinity ◽

Hemoglobin A ◽

Quaternary Structures

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Chemical characterization of cyanogen bromide fragments from the β-chain of human complement factor C3

FEBS Letters ◽

10.1016/0014-5793(84)80289-6 ◽

1984 ◽

Vol 169 (1) ◽

pp. 57-62 ◽

Cited By ~ 14

Author(s):

Åke Lundwall ◽

Ulf Hellman ◽

Gösta Eggertsen ◽

John Sjöquist

Keyword(s):

Cyanogen Bromide ◽

Chemical Characterization ◽

Complement Factor ◽

Human Complement ◽

Complement Factor C3 ◽

Β Chain

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Hemoglobin Hirose: α2β237(C3) Tryptophan Yielding Serine

Blood ◽

10.1182/blood.v38.6.730.730 ◽

1971 ◽

Vol 38 (6) ◽

pp. 730-738 ◽

Cited By ~ 41

Author(s):

KOTARO YAMAOKA

Keyword(s):

Family Members ◽

Chemical Characterization ◽

The Third ◽

The Family ◽

Western Japan ◽

Slow Moving ◽

Clinically Significant ◽

Β Chain ◽

Screening Survey

Abstract During an electrophoretic screening survey for hemoglobinopathies in western Japan, a slow-moving variant of hemoglobin A, to be designated hemoglobin Hirose, was found in a family of Japanese origin. Chemical characterization of hemoglobin Hirose revealed that tryptophan at the 37th position of the β-chain was replaced by serine, the third residue of C-helix of the β-chain involving contacts between αl and β2 subunits. Even though the oxygen equilibrium of this hemoglobin was abnormal, none of the family members showed any clinically significant symptoms.

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