scholarly journals Effect of Phytohemagglutinin on Enzymes of Thymidine Salvage Pathway of Cultured Chronic Lymphatic Leukemic Lymphocytes

Blood ◽  
1970 ◽  
Vol 35 (2) ◽  
pp. 236-242 ◽  
Author(s):  
YALE RABINOWITZ ◽  
POLLY WONG ◽  
BETTY A. WILHITE
Keyword(s):  
Cell Culture ◽  
Dna Polymerase ◽  
Enzyme Activities ◽  
Kinase Activity ◽  
Cell Formation ◽  
Polymerase Activity ◽  
Blast Cell ◽  
Salvage Pathway ◽  
Culture Cells ◽  
Cells Cultured

Abstract N-L and CLL-L prior to cell culture gave evidence of high activities of TdR phosphorylase, TMP and TDP kinases, while TdR kinase and phosphatase activities were relatively low. Cells cultured without PHA showed no appreciable alteration in enzyme activities during three to five days of culture. Response to PHA with blast-cell formation in cultures of N-L or CLL-L (low-count cases) was associated with increased TdR kinase activity which became prominent at the time of increased DNA polymerase activity and DNA synthesis during the second to third day of culture. Cells from cases of CLL-L with high WBC counts which failed to respond to PHA showed no increase in TdR kinase activity but did show increased phosphatase activity. Increased TdR activity and increased DNA polymerase activity may both be needed for increased synthesis of DNA to occur in lymphocytes responding to PHA.

Effect of cell culture models on the evaluation of anticancer activity and mechanism analysis of the potential bioactive compound, iturin A, produced by Bacillus subtilis

2019 ◽  
Vol 10 (3) ◽  
pp. 1478-1489 ◽  
Author(s):  
Haobin Zhao ◽  
Xixi Zhao ◽  
Shuzhen Lei ◽  
Yawen Zhang ◽  
Dongyan Shao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bacillus Subtilis ◽  
3D Culture ◽  
Bioactive Compound ◽  
Mechanism Analysis ◽  
Iturin A ◽  
Culture Cells ◽  
Culture Models ◽  
Cell Culture Models ◽  
Cells Cultured

Compared to 2D culture, cells cultured in 3D culture were more resistant to iturin A from Bacillus.

Hormonal dependency of cerebral meningiomas

1990 ◽  
Vol 73 (5) ◽  
pp. 750-755 ◽  
Author(s):  
Eric F. Adams ◽  
Uwe M. H. Schrell ◽  
Rudolf Fahlbusch ◽  
Paul Thierauf
Keyword(s):  
Cell Culture ◽  
Dna Polymerase ◽  
Cell Cultures ◽  
Polymerase Activity ◽  
Proliferative Potential ◽  
Content Type ◽  
Androgen Antagonists ◽  
Epidermal Growth ◽  
Meningioma Cell

✓ Cell culture and biochemical techniques have been employed to examine the effects of steroids, bromocriptine, and epidermal growth factor (EGF) on the growth and proliferative potential of meningiomas. In cell culture, the growth of meningiomas was not altered by progestogens, antiprogestogens, or 17β-estradiol. The progestogen, norethisterone, had no effect on the uptake by meningioma cell cultures of 3H-thymidine. Furthermore, cytosolic deoxyribonucleic acid (DNA) polymerase activity of meningiomas did not correlate with the progesterone receptor status of the same tumors. In contrast, the androgen antagonists, cyproterone acetate and 11-α-hydroxyprogesterone, and the dopamine agonist, bromocriptine, all inhibited the in vitro growth of meningioma cells. The growth of meningioma cell cultures was stimulated by EGF, and there was a positive correlation between the EGF content and DNA polymerase activity in meningioma cytosols. These results demonstrate that female sex steroids do not influence growth of meningiomas in vitro, whereas antiandrogens and bromocriptine have an antiproliferative effect. Consequently, bromocriptine and antiandrogens may have a role in the medical treatment of meningiomas. In addition, these results suggest that EGF may be involved in the genesis and/or progression of meningiomas.

Facile and Sensitive Fluorescence Assay of DNA Polymerase Activity Using Cu2+ and Ascorbate as Signal Developers

2019 ◽  
Vol 4 (8) ◽  
pp. 2398-2403 ◽  
Author(s):  
Xingxing Zhang ◽  
Qiang Liu ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Fluorescence Assay

Suggestive Evidence for an Oncorna-Virus-Specific DNA Polymerase from C-Type Particles of Bovine Leukosis

1974 ◽  
Vol 29 (1-2) ◽  
pp. 72-75 ◽  
Author(s):  
B. Dietzschold ◽  
O.R. Kaaden ◽  
S. Ueberschaer ◽  
F. Weiland ◽  
O. C. Straub
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity ◽  
Friend Virus ◽  
Leukaemia Virus ◽  
Virus Particles ◽  
Virus Rna ◽  
Feline Leukaemia Virus ◽  
Bovine Leukosis ◽  
Bovine Lymphocytes ◽  
Viral Preparation

Abstract Typical C-type oncorna virus particles as shown by electron microscopy have been purified from the supernatant of cultured lymphocytes from bovine leukosis. In the purified C-particle fraction a DNA-polymerase activity was detected. Using several synthetic RNA-or DNA-homopolymers and 70S Friend virus RNA the template response of this bovine leukosis cell particle DNA polymerase was compared with those of feline leukaemia virus DNA polymerase and DNA polymerase from normal bovine lymphocytes. The DNA polymerase detected in the viral preparation of bovine leukosis is suggested to be an oncorna-virus-specific enzyme.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

scholarly journals Human Cytomegalovirus Inhibitor AL18 Also Possesses Activity against Influenza A and B Viruses

2012 ◽  
Vol 56 (11) ◽  
pp. 6009-6013 ◽  
Author(s):  
Giulia Muratore ◽  
Beatrice Mercorelli ◽  
Laura Goracci ◽  
Gabriele Cruciani ◽  
Paul Digard ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Dna Polymerase ◽  
Human Cytomegalovirus ◽  
Influenza A ◽  
Molecular Model ◽  
Polymerase Activity

ABSTRACTAL18, an inhibitor of human cytomegalovirus DNA polymerase, was serendipitously found to also block the interaction between the PB1 and PA polymerase subunits of influenza A virus. Furthermore, AL18 effectively inhibited influenza A virus polymerase activity and the overall replication of influenza A and B viruses. A molecular model to explain the binding of AL18 to both cytomegalovirus and influenza targets is proposed. Thus, AL18 represents an interesting lead for the development of new antivirals.

DNA polymerase activity of cultured normal and leukemic lymphocytes *1Response to phytohemagglutinin

1969 ◽  
Vol 57 (2-3) ◽  
pp. 257-262 ◽  
Author(s):  
Y RABINOWITZ ◽  
I MCCLUSKEY ◽  
P WONG ◽  
B WILHITE
Keyword(s):  
Dna Polymerase ◽  
Polymerase Activity
Sign in / Sign up

Export Citation Format

Share Document