Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs

Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of Hh requires its oligomerization. Here we show that Hh is secreted in the form of exovesicles. These are derived by the endocytic delivery of cell surface Hh to multivesicular bodies (MVBs) via an endosomal sorting complex required for transport (ECSRT)–dependent process. Perturbations of ESCRT proteins have a selective effect on long-range Hh signaling in Drosophila wing imaginal discs. Of importance, oligomerization-defective Hh is inefficiently incorporated into exovesicles due to its poor endocytic delivery to MVBs. These results provide evidence that nanoscale organization of Hh regulates the secretion of Hh on ESCRT-derived exovesicles, which in turn act as a vehicle for long-range signaling.

Download Full-text

Wingless signaling and the control of cell shape in Drosophila wing imaginal discs

Developmental Biology ◽

10.1016/j.ydbio.2009.07.013 ◽

2009 ◽

Vol 334 (1) ◽

pp. 161-173 ◽

Cited By ~ 34

Author(s):

Thomas J. Widmann ◽

Christian Dahmann

Keyword(s):

Cell Shape ◽

Imaginal Discs ◽

Drosophila Wing ◽

Wingless Signaling ◽

Wing Imaginal Discs

Download Full-text

Hedgehog mediated degradation of Ihog adhesion proteins modulates cell segregation in Drosophila wing imaginal discs

Nature Communications ◽

10.1038/s41467-017-01364-z ◽

2017 ◽

Vol 8 (1) ◽

Cited By ~ 10

Author(s):

Elaine Y. C. Hsia ◽

Ya Zhang ◽

Hai Son Tran ◽

Agnes Lim ◽

Ya-Hui Chou ◽

...

Keyword(s):

Imaginal Discs ◽

Adhesion Proteins ◽

Drosophila Wing ◽

Wing Imaginal Discs ◽

Cell Segregation

Download Full-text

Towards Long Term Cultivation of Drosophila Wing Imaginal Discs In Vitro

PLoS ONE ◽

10.1371/journal.pone.0107333 ◽

2014 ◽

Vol 9 (9) ◽

pp. e107333 ◽

Cited By ~ 22

Author(s):

Björn Handke ◽

János Szabad ◽

Peter V. Lidsky ◽

Ernst Hafen ◽

Christian F. Lehner

Keyword(s):

Imaginal Discs ◽

Drosophila Wing ◽

Wing Imaginal Discs

Download Full-text

Modulation of Hedgehog target gene expression by the Fused serine–threonine kinase in wing imaginal discs

Mechanisms of Development ◽

10.1016/s0925-4773(98)00130-0 ◽

1998 ◽

Vol 78 (1-2) ◽

pp. 17-31 ◽

Cited By ~ 64

Author(s):

Georges Alves ◽

Bernadette Limbourg-Bouchon ◽

Hervé Tricoire ◽

Jeanine Brissard-Zahraoui ◽

Claudie Lamour-Isnard ◽

...

Keyword(s):

Gene Expression ◽

Target Gene ◽

Imaginal Discs ◽

Serine Threonine Kinase ◽

Threonine Kinase ◽

Target Gene Expression ◽

Wing Imaginal Discs

Download Full-text

Transcriptome dynamics during metamorphosis of imaginal discs into wings and thoracic dorsum in Apis mellifera castes

BMC Genomics ◽

10.1186/s12864-021-08040-z ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Michelle Prioli Miranda Soares ◽

Daniel Guariz Pinheiro ◽

Flávia Cristina de Paula Freitas ◽

Zilá Luz Paulino Simões ◽

Márcia Maria Gentile Bitondi

Keyword(s):

Gene Expression ◽

Expression Profiles ◽

Imaginal Discs ◽

Rna Seq ◽

Transition Analysis ◽

Holometabolous Insect ◽

Complex Anatomy ◽

Gene Expression Dynamics ◽

Wing Discs ◽

Developmental Phases

Abstract Background Much of the complex anatomy of a holometabolous insect is built from disc-shaped epithelial structures found inside the larva, i.e., the imaginal discs, which undergo a rapid differentiation during metamorphosis. Imaginal discs-derived structures, like wings, are built through the action of genes under precise regulation. Results We analyzed 30 honeybee transcriptomes in the search for the gene expression needed for wings and thoracic dorsum construction from the larval wing discs primordia. Analyses were carried out before, during, and after the metamorphic molt and using worker and queen castes. Our RNA-seq libraries revealed 13,202 genes, representing 86.2% of the honeybee annotated genes. Gene Ontology analysis revealed functional terms that were caste-specific or shared by workers and queens. Genes expressed in wing discs and descendant structures showed differential expression profiles dynamics in premetamorphic, metamorphic and postmetamorphic developmental phases, and also between castes. At the metamorphic molt, when ecdysteroids peak, the wing buds of workers showed maximal gene upregulation comparatively to queens, thus underscoring differences in gene expression between castes at the height of the larval-pupal transition. Analysis of small RNA libraries of wing buds allowed us to build miRNA-mRNA interaction networks to predict the regulation of genes expressed during wing discs development. Conclusion Together, these data reveal gene expression dynamics leading to wings and thoracic dorsum formation from the wing discs, besides highlighting caste-specific differences during wing discs metamorphosis.

Download Full-text

Promoter Proximal Pausing Limits Tumorous Growth Induced by the Yki Transcription Factor in Drosophila

Genetics ◽

10.1534/genetics.120.303419 ◽

2020 ◽

Vol 216 (1) ◽

pp. 67-77 ◽

Cited By ~ 1

Author(s):

Sanket Nagarkar ◽

Ruchi Wasnik ◽

Pravallika Govada ◽

Stephen Cohen ◽

L. S. Shashidhara

Keyword(s):

Gene Expression ◽

Rna Polymerase Ii ◽

Gene Expression Regulation ◽

Regulation Of Gene Expression ◽

Elongation Factor ◽

Imaginal Discs ◽

Link Type ◽

Genome Wide ◽

Wing Imaginal Discs ◽

Tumorous Growth

Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we show that RNAi-mediated depletion of bin3 or Hexim of the 7SK snRNP complex or depletion of individual components of the NELF complex enhances Yki-driven growth, leading to neoplastic transformation of Drosophila wing imaginal discs. We also show that increased CDK9 expression cooperates with Yki in driving neoplastic growth. Interestingly, overexpression of CDK9 on its own or in the background of depletion of one of the components of 7SK snRNP or the NELF complex necessarily, and specifically, needed Yki overexpression to cause tumorous growth. Genome-wide gene expression analyses suggested that deregulation of protein homeostasis is associated with tumorous growth of wing imaginal discs. As both Fat/Hippo/Yki pathway and PPP are highly conserved, our observations may provide insights into mechanisms of oncogenic function of YAP—the ortholog of Yki in humans.

Download Full-text