scholarly journals Genetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thaliana

BMC Genetics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 59 ◽  
Author(s):  
Carolina Serrano ◽  
Javiera González-Cruz ◽  
Francisca Jauregui ◽  
Consuelo Medina ◽  
Pablo Mancilla ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Histological Studies ◽  
Systemic Movement

scholarly journals Susceptibility and Symptom Development in Arabidopsis thaliana to Tobacco mosaic virus Is Influenced by Virus Cell-to-Cell Movement

2000 ◽  
Vol 13 (10) ◽  
pp. 1139-1144 ◽  
Author(s):  
Christopher D. Dardick ◽  
Sheetal Golem ◽  
James N. Culver
Keyword(s):  
Arabidopsis Thaliana ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Cell Movement ◽  
Recessive Gene ◽  
Defense Responses ◽  
Systemic Movement ◽  
Pathogenesis Related ◽  
Symptom Phenotype ◽  
Dominant Genes

To identify host factors that regulate susceptibility to Tobacco mosaic virus(TMV), 14 Arabidopsis thaliana ecotypes were screened for their ability to support TMV systemic movement. The susceptibility phenotypes observed included one ecotype that permitted rapid TMV movement accompanied by symptoms, nine ecotypes that allowed a slower intermediate rate of systemic movement without symptoms, and four ecotypes that allowed little or no systemic TMV movement. Molecular comparisons between ecotypes representing the rapid (Shahdara), intermediate (Col-1), and slow (Tsu-1) movement phenotypes revealed a positive correlation between the ability of TMV to move cell to cell and its speed of systemic movement. Additionally, protoplasts prepared from all three ecotypes supported similar levels of TMV replication, indicating that viral replication did not account for differences in systemic movement. Furthermore, induction of the pathogenesis-related genes PR-1 and PR-5 occurred only in the highly susceptible ecotype Shahdara, demonstrating that reduced local and systemic movement in Col-1 and Tsu-1 was not due to the activation of known host defense responses. Genetic analysis of F2 progeny derived from crosses made between Shahdara and Tsu-1 or Col-1 and Tsu-1 showed the faster cell-to-cell movement phenotypes of Shahdara and Col-1 segregated as single dominant genes. In addition, the Shahdara symptom phenotype segregated independently as a single recessive gene. Taken together, these findings suggest that, within Arabidopsis ecotypes, at least two genes modulate susceptibility to TMV.

scholarly journals Specific Enrichment of miRNAs in Arabidopsis thaliana Infected with Tobacco mosaic virus

DNA Research ◽  
2007 ◽  
Vol 14 (5) ◽  
pp. 227-233 ◽  
Author(s):  
Yuko Tagami ◽  
Naoko Inaba ◽  
Natsumaro Kutsuna ◽  
Yukio Kurihara ◽  
Yuichiro Watanabe
Keyword(s):  
Arabidopsis Thaliana ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus

scholarly journals Evaluation of Seed Transmission of Turnip yellow mosaic virus and Tobacco mosaic virus in Arabidopsis thaliana

2000 ◽  
Vol 90 (11) ◽  
pp. 1233-1238 ◽  
Author(s):  
F. M. de Assis Filho ◽  
J. L. Sherwood
Keyword(s):  
Arabidopsis Thaliana ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Seed Coat ◽  
Virus Transmission ◽  
Systemic Infection ◽  
Seed Transmission ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Turnip Yellow Mosaic Virus

The mechanism of virus transmission through seed was studied in Arabidopsis thaliana infected with Turnip yellow mosaic virus (TYMV) and Tobacco mosaic virus (TMV). Serological and biological tests were conducted to identify the route by which the viruses reach the seed and subsequently are located in the seed. Both TYMV and TMV were detected in seed from infected plants, however only TYMV was seed-transmitted. This is the first report of transmission of TYMV in seed of A. thaliana. Estimating virus seed transmission by grow-out tests was more accurate than enzyme-linked immunosorbent assay due to the higher frequency of antigen in the seed coat than in the embryo. Virus in the seed coat did not lead to seedling infection. Thus, embryo invasion is necessary for seed transmission of TYMV in A. thaliana. Crosses between healthy and virus-infected plants indicated that TYMV from either the female or the male parent could invade the seed. Conversely, invasion from maternal tissue was the only route for TMV to invade the seed. Pollination of flowers on healthy A. thaliana with pollen from TYMV-infected plants did not result in systemic infection of healthy plants, despite TYMV being carried by pollen to the seed.

Analysis of local and systemic spread of the crucifer-infecting TMV-Cg virus in tobacco and several Arabidopsis thaliana ecotypes

2003 ◽  
Vol 30 (4) ◽  
pp. 401 ◽  
Author(s):  
Patricio Arce-Johnson ◽  
Consuelo Medina ◽  
Hal S. Padgett ◽  
Wilson Huanca ◽  
Carmen Espinoza
Keyword(s):  
Arabidopsis Thaliana ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Host Interactions ◽  
Nicotiana Tabacum L ◽  
Systemic Spread ◽  
Movement Protein Gene ◽  
Local Movement

The crucifer-infecting tobacco mosaic virus, TMV-Cg, infects Arabidopsis thaliana (L.) Heynh. efficiently without causing severe symptoms. The systemic spread of TMV-Cg in Arabidopsis was evaluated in 14�ecotypes. Five days after inoculation, TMV-Cg was detected in apical leaves of 8 out of 14 ecotypes. As expected, the spread of TMV-Cg in the ecotypes tested was considerably faster than that of tobacco mosaic virus (TMV-U1). To study the participation of viral proteins in the TMV-Cg-induced infection, a complete genomic cDNA of TMV-Cg was cloned. The role of TMV-Cg movement protein in systemic spread was tested with a hybrid virus, constructed from the TMV-U1 genome and the TMV-Cg movement protein gene. Contrary to expectations, the systemic spread of this hybrid in Arabidopsis was similar to that of TMV-U1. The failure of the hybrid virus to spread at rates similar to those of TMV-Cg was not due to restrictions in local movement. In tobacco (Nicotiana tabacum L.), the hybrid virus spread efficiently and induced systemic mosaic symptoms characteristic of TMV-U1. The TMV-Cg cDNA clone provides an attractive tool to study virus–host interactions.

scholarly journals Tobacco mosaic virus Induced Alterations in the Gene Expression Profile of Arabidopsis thaliana

2003 ◽  
Vol 16 (8) ◽  
pp. 681-688 ◽  
Author(s):  
Sheetal Golem ◽  
James N. Culver
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Infection Process ◽  
Cellular Processes ◽  
Genes Expression ◽  
Identify Gene Expression

In this study, mRNA profiles generated from cDNA microarrays were used to identify gene expression changes in Arabidopsis thaliana ecotype Shahdara infected with Tobacco mosaic virus (TMV). Shahdara is a susceptible TMV host, permitting rapid accumulations of virus in both inoculated and systemic tissues, accompanied by defined disease symptoms that include stunting, necrosis, and leaf curling. Gene expression profiles were monitored in whole tissues of inoculated leaves at four days postinoculation (dpi) and in systemically infected leaves at 14 dpi. Microarrays contained cDNAs representing between 8,000 and 10,000 Arabidopsis genes. Expression analysis identified 68 genes that displayed significant and consistent changes in expression levels, either up or down, in either TMV inoculated or systemically infected tissues, or both. Identified TMV-responsive genes encode a diverse array of functional proteins that include transcription factors, antioxidants, metabolic enzymes, and transporters. Thus, the TMV infection process has a significant impact on a wide array of cellular processes that likely reflect the biochemical and physiological changes involved in the development of this disease syndrome.

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