scholarly journals An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease

2010 ◽  
Vol 9 (1) ◽  
pp. 59 ◽  
Author(s):  
Sanjay K Singh ◽  
Vinayak R Tripathi ◽  
Rakesh K Jain ◽  
Surendra Vikram ◽  
Satyendra K Garg
Keyword(s):  
Heavy Metal ◽  
Bacillus Cereus ◽  
Heavy Metal Resistant

scholarly journals Microbacterium sp. MRS-1, a potential bacterium for cobalt reduction and synthesis of less/non-toxic cobalt oxide nanoparticles (Co3O4)

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Sathyavathi Sundararaju ◽  
Manjula Arumugam ◽  
Prakash Bhuyar
Keyword(s):  
Heavy Metal ◽  
Cobalt Oxide ◽  
Metal Oxide Nanoparticles ◽  
Industrial Effluent ◽  
Industrial Applications ◽  
Resistant Bacterium ◽  
Oxide Nanoparticles ◽  
Cobalt Metal ◽  
Cobalt Oxide Nanoparticles ◽  
Heavy Metal Resistant

Abstract Background Detoxification of heavy metal pollutants in wastewater has become a serious problem to surrounding environment. This research was conducted to utilize a potential heavy metal-resistant bacterium for the remediation of cobalt metal and simultaneous synthesis of cobalt oxide nanoparticles in the form of powder for various industrial applications. Metal oxide nanoparticles have great applications in electrochemical devices such as supercapacitors, biosensors, and batteries. Method A heavy metal-resistant bacterium Microbacterium sp. MRS-1 isolated from electroplating industrial effluent reduced cobalt ions from an initial concentration of 200 mg/L to 26 mg/L were analyzed by atomic absorption spectroscopy. Instrumental analysis of bacterially synthesized Co3O4 has been characterized. Cytotoxicity of synthesized nanoparticles was assessed by MTT assay. Results Microbacterium sp. MRS-1 isolated from electroplating industrial effluent was found to be suitable for cobalt oxide nanoparticles as it showed tolerance towards high concentration of metal. The nutrient broth containing metal solution and Microbacterium sp. MRS-1 showed color change from light pink to dark pink indicated the formation of extracellular nanoparticles. It also converted soluble cobalt salts into less soluble cobalt oxide nanoparticles outside the cell which allows easy recovery of nanoparticles without the destruction of cells and simultaneous detoxification of toxic metal ions. Electron microscopic imaging verified that nanoparticles were predominantly surrounding the bacterial cells and SEM imaging revealed that the produced particles were in the range of 10–100 nm in size. XRD spectrum exhibited 2θ values were corresponding to cubic face-centered cobalt oxide (Co3O4) nanoparticles. Conclusion The present study investigated new prospective for eco-friendly detoxification of toxic heavy metal Co from metal-polluted sites and the production of cobalt oxide nanoparticles in powder form for clinical and other industrial applications.

scholarly journals Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Grab Sampling ◽  
Antibiotic Resistance Pattern ◽  
Antibiotic Susceptibility Test ◽  
Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 

scholarly journals Taking nature into lab: biomineralization by heavy metal resistant streptomycetes in soil

2013 ◽  
Vol 10 (2) ◽  
pp. 2345-2375 ◽  
Author(s):  
E. Schütze ◽  
A. Weist ◽  
M. Klose ◽  
T. Wach ◽  
M. Schumann ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Control Soil ◽  
Natural Conditions ◽  
Laboratory Methods ◽  
Dominant Property ◽  
Resistant Strains ◽  
Heavy Metal Resistant ◽  
And Control

Abstract. Biomineralization by heavy metal resistant streptomycetes was tested to evaluate the potential influence on metal mobilities in soil. Thus, we designed an experiment adopting conditions from classical laboratory methods to natural conditions prevailing in metal-rich soils with media spiked with heavy metals, soil agar, and nutrient enriched or unamended soil incubated with the bacteria. As a result, all strains were able to form struvite minerals on tryptic soy broth (TSB) media supplemented with AlCl2, MnCl2 and CuSO4, as well as on soil agar. Some strains additionally formed struvite on nutrient enriched contaminated and control soil, as well as on metal contaminated soil without addition of media components. In contrast, switzerite was exclusively formed on minimal media spiked with MnCl2 by four heavy metal resistant strains, and on nutrient enriched control soil by one strain. Hydrated nickel hydrogen phosphate was only crystallized on complex media supplemented with NiSO4 by most strains. Thus, mineralization is a~dominant property of streptomycetes, with different processes likely to occur under laboratory conditions and sub-natural to natural conditions. This new understanding may be transferred to formation of minerals in rock and sediment evolution, to ore deposit formation, and also might have implications for our understanding of biological metal resistance mechanisms. We assume that biogeochemical cycles, nutrient storage and metal resistance might be affected by formation and re-solubilization of minerals like struvite in soil at microscale.

Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp. D4 and cloning of its cnb genes

2011 ◽  
Vol 65 (6) ◽  
pp. 871-876 ◽  
Author(s):  
Zhiguo Wu ◽  
Yalong Liu ◽  
Ji Zhang ◽  
Weiliang Shen ◽  
Wenxiang Lu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistant

Heavy-Metal Resistant Actinomycetes

2005 ◽  
pp. 757-767
Author(s):  
M. Siñeriz Louis ◽  
J. M. Benito ◽  
V. H. Albarracín ◽  
Thierry Lebeau ◽  
M. J. Amoroso ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistant
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