Effects of tea saponin on glucan conversion and bonding behaviour of cellulolytic enzymes during enzymatic hydrolysis of corncob residue with high lignin content

Abstract Background Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei. Reported literatures about Cip1 mainly focused on the regulation of Cip1 and its possible enzyme activities, but the effect of Cip1 on the enzymatic hydrolysis of lignocellulose and possible mechanism have not still been reported. Results In this study, Cip1 from T. reesei was cloned, expressed and purified, and its effects on enzymatic hydrolysis of several different pretreated lignocellulose were investigated. It was found that Cip1 could promote the enzymatic hydrolysis of pretreated lignocellulose, and the promoting effect was significantly better than that of bovine serum albumin (BSA). And especially for the lignocellulosic substrate with high lignin content such as liquid hot water pretreated corn stover and corncob residue, the promoting effect of Cip1 was even better than that of the commercial cellulase when adding equal amount protein. It was also showed that the metal ions Zn2+ and Cu2+ influenced the promoting effect on enzymatic hydrolysis. The Cip1 protein had no lyase activity, but it could destroy the crystal structure of cellulose and reduce the non-productive adsorption of cellulase on lignin, which partly interpreted the promoting effect of Cip1 on enzymatic hydrolysis of lignocellulose. Conclusion The Cip1 from T. reesei could significantly promote the enzymatic hydrolysis of pretreated lignocellulose, and the promotion of Cip1 was even higher than that of commercial cellulase in the enzymatic hydrolysis of the substrates with high lignin content. This study will help us to better optimize cellulase to improve its ability to degrade lignocellulose, thereby reducing the cost of enzymes required for enzymatic hydrolysis.

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Impact of centrifugation treatment on enzymatic hydrolysis of cellulose and xylan in poplar fibers with high lignin content

Bioresource Technology ◽

10.1016/j.biortech.2020.123866 ◽

2020 ◽

Vol 316 ◽

pp. 123866

Author(s):

Hongkang Fu ◽

Wenxuan Mo ◽

Xiaoning Shen ◽

Bo Li

Keyword(s):

Enzymatic Hydrolysis ◽

Lignin Content ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of ◽

High Lignin Content

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An approach to cellulase recovery from enzymatic hydrolysis of pretreated sugarcane bagasse with high lignin content

Biocatalysis and Biotransformation ◽

10.3109/10242422.2016.1168816 ◽

2015 ◽

Vol 33 (5-6) ◽

pp. 287-297 ◽

Cited By ~ 5

Author(s):

Leyanis Mesa ◽

Erenio González ◽

Cristóbal Cara ◽

Eulogio Castro ◽

Solange I. Mussatto

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Lignin Content ◽

Pretreated Sugarcane Bagasse ◽

Cellulase Recovery ◽

Hydrolysis Of ◽

High Lignin Content

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Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

Scientific Reports ◽

10.1038/s41598-020-80719-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chiranjeevi Thulluri ◽

Ravi Balasubramaniam ◽

Harshad Ravindra Velankar

Keyword(s):

Enzymatic Hydrolysis ◽

Rice Straw ◽

Deep Eutectic Solvent ◽

Solvent System ◽

Cyclic Ether ◽

Cellulolytic Enzymes ◽

Ammonium Bromide ◽

Selective Delignification ◽

Cellulose Iβ ◽

Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

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Strategies to Enhance the Enzymatic Hydrolysis of Pretreated Softwood with High Residual Lignin Content

Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals ◽

10.1007/978-1-59259-991-2_90 ◽

2009 ◽

pp. 1069-1079 ◽

Cited By ~ 3

Author(s):

Xuejun Pan ◽

Dan Xie ◽

Neil Gilkes ◽

David J. Gregg ◽

Jack N. Saddler

Keyword(s):

Enzymatic Hydrolysis ◽

Lignin Content ◽

Residual Lignin ◽

Hydrolysis Of

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Reduction of surface area of lignin improves enzymatic hydrolysis of cellulose from hydrothermally pretreated wheat straw

RSC Advances ◽

10.1039/c4ra06926a ◽

2014 ◽

Vol 4 (69) ◽

pp. 36591-36596 ◽

Cited By ~ 9

Author(s):

M. H. Sipponen ◽

V. Pihlajaniemi ◽

O. Pastinen ◽

S. Laakso

Keyword(s):

Enzymatic Hydrolysis ◽

Wheat Straw ◽

Surface Area ◽

Lignin Content ◽

Cellulose Conversion ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Pretreated Wheat Straw ◽

Hydrolysis Of

24 h enzymatic hydrolysis (15 FPU g−1) of solid residues from wheat straw autohydrolysis. Cellulose conversion as a function of lignin content (left) or lignin surface area (right) in solid residues.

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A Comparative Study of Microcrystalline Cellulose Isolated from the Pod Husk and Stalk of Fluted Pumpkin

Chemical Science International Journal ◽

10.9734/csji/2018/v25i430074 ◽

2019 ◽

pp. 1-11

Author(s):

C. C. Nwajiobi ◽

J. O. E. Otaigbe ◽

O. Oriji

Keyword(s):

Physicochemical Properties ◽

Microcrystalline Cellulose ◽

Lignin Content ◽

Cellulose Derivatives ◽

Cellulose Content ◽

Alternative Source ◽

Fluted Pumpkin ◽

Hydrolysis Of ◽

High Lignin Content ◽

High Cellulose

Microcrystalline celluloses (MCC) were prepared from α-celluloses obtained from fluted pumpkin stalk and pod. The substrates were subjected to treatment with 2% (w/v) NaOH, 3.5% (w/v) NaOCl and 17.5% (w/v) NaOH solutions respectively to obtain alpha celluloses. Acid hydrolysis of the alpha-celluloses using 2.5 N hydrochloric acid were carried out. The study evaluates and compares the physicochemical properties of microcrystalline cellulose obtained from the pod and stalk of fluted pumpkin. Composition of cellulose, hemicellulose and lignin were also determined. Results showed cellulose; hemicellulose and lignin content of the pod husk and stalk were 49%, 26%, 9% and 41%, 24%, 26%, respectively. The morphology of the hydrolyzed MCCs’ were investigated using scanning electron microscopy (SEM) and the results revealed the stalk (FS-MCC) to have an individual rod-like shaped fiber when compared with flat-shaped large aggregated forms of the pod (FP-MCC). The particles sizes were also uneven with FP-MCC (6.689 µm) having larger particle sizes than FS-MCC (5.538 µm). The high cellulose content of the pod husk shows that the applications may be extended in the production of other cellulose derivatives while the high lignin content of the stalk reveals other alternative source of producing lignin in the making of textile dyes, coating and other agricultural chemical. Pod MCC (FP-MCC) had better physicochemical properties than the stalk MCC (FS-MCC).

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