Soybean allergy: IgE epitopes of glycinin (Gly m 6), an important soybean allergen

Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82–24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

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Analysis of the IgE epitope profile of soybean allergen Gly m 4

Clinical and Translational Allergy ◽

10.1186/2045-7022-4-s2-p42 ◽

2014 ◽

Vol 4 (S2) ◽

Author(s):

Felix Husslik ◽

Diana Mittag ◽

Christian Seutter von Loetzen ◽

Maximilian Hartl ◽

Lothar Vogel ◽

...

Keyword(s):

Ige Epitope ◽

Soybean Allergen

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Gly m 5/Gly m 8 fusion component for diagnosing soybean allergy

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2017.12.750 ◽

2018 ◽

Vol 141 (2) ◽

pp. AB237

Author(s):

Nobuyuki Maruyama ◽

Sakura Sato ◽

Cerrone Cabanos ◽

Akira Tanaka ◽

Komei Ito ◽

...

Keyword(s):

Soybean Allergy

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Mitigation of Soybean Allergy by Development of Low Allergen Content Seeds

ACS Symposium Series - Food Contaminants ◽

10.1021/bk-2008-1001.ch028 ◽

2008 ◽

pp. 431-445 ◽

Cited By ~ 2

Author(s):

Eliot M. Herman

Keyword(s):

Soybean Allergy ◽

Allergen Content

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Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2

International Journal of Food Engineering ◽

10.1515/ijfe-2018-0116 ◽

2018 ◽

Vol 15 (1-2) ◽

Author(s):

Yuan Zhao ◽

Shiyao Zhang ◽

Xiaodong Zhang ◽

Li Pan ◽

Nan Bao ◽

...

Keyword(s):

Mass Spectrometry ◽

Enzymatic Hydrolysis ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Time Dependent ◽

Dependent Manner ◽

Intestinal Epithelial ◽

Intracellular Accumulation ◽

Novel Therapeutic ◽

Soybean Allergen

AbstractDissecting the inhibited variation of allergen absorption could contribute to the development of novel therapeutic or preventive treatments for food/feed allergies. This study investigated the effects of fructooligosaccharide (FOS) on the absorption, intracellular accumulation of intact or hydrolysed β-conglycinin in porcine intestinal epithelial cells (IPEC-J2). As demonstrated by ELISA and immunoblotting, β-conglycinin was absorbed in a dose- and time-dependent manner (p < 0.05). Actually, β-conglycinin was easily transported and absorbed after enzymatic hydrolysis. Three peptides (52 kDa, 30 kDa and 25 kDa) were produced during transcellular absorption of intact or hydrolysed β-conglycinin. FOS inhibited the absorption of β-conglycinin, especially the 52 and 30 kDa peptides. The immunoreactive peptides derived from the 52, 35 or 22 kDa peptides were the substrings of the known epitopes determined by mass spectrometry and bioinformatic analyses. These results indicate that FOS can efficiently inhibit the absorption of 52 and 30 kDa peptides derived from β-conglycinin.

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The Development of Highly Specific and Sensitive Primers for the Detection of Potentially Allergenic Soybean (Glycine max) Using Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD)

Foods ◽

10.3390/foods9040423 ◽

2020 ◽

Vol 9 (4) ◽

pp. 423 ◽

Cited By ~ 1

Author(s):

Stefanie M. Allgöwer ◽

Chris A. Hartmann ◽

Thomas Holzhauser

Keyword(s):

Glycine Max ◽

Detection Method ◽

Lateral Flow ◽

Isothermal Amplification ◽

Loop Mediated Isothermal Amplification ◽

Lateral Flow Dipstick ◽

Soybean Allergy ◽

Systematic Development ◽

Multicopy Genes ◽

Selection Of

The soybean (Glycine max) has been recognized as a frequent elicitor of food allergy worldwide. A lack of causative immunotherapy of soybean allergy makes soybean avoidance essential. Therefore, sensitive and specific methods for soybean detection are needed to allow for soybean verification in foods. Loop-mediated isothermal amplification (LAMP) represents a rapid and simple DNA-based detection method principally suitable for field-like applications or on-site analytical screening for allergens during the manufacturing of foods. This work describes the systematic development and selection of suitable LAMP primers based on soybean multicopy genes. The chemistry applied allows for a versatile detection of amplified DNA, using either gel electrophoresis, fluorescence recording, or a simple Lateral Flow Dipstick (LFD). LAMP based on the ORF160b gene was highly specific for the soybean and may allow for a detection level equivalent to approximately 10 mg soy per kg food. Various soybean cultivars were detectable at a comparable level of sensitivity. LAMP combined with LFD-like detection facilitates a simple, highly specific and sensitive detection of the soybean without the need for expensive analytical equipment. In contrast to the majority of antibody-based methods for soybean detection, all identified primer sequences and optimized protocols are disclosed and broadly available to the community.

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