scholarly journals Receptor guanylyl cyclase B-mediated cGMP signalling controls axon bifurcation of cranial sensory ganglion neurons at the embryonic hindbrain

2013 ◽  
Vol 14 (S1) ◽  
Author(s):  
Gohar Ter-Avetisyan ◽  
Fritz G Rathjen ◽  
Hannes Schmidt
Keyword(s):  
Guanylyl Cyclase ◽  
Sensory Ganglion ◽  
Cgmp Signalling ◽  
Ganglion Neurons

scholarly journals Lack of effect of ODQ does not exclude cGMP signalling via NO-sensitive guanylyl cyclase

2013 ◽  
Vol 170 (2) ◽  
pp. 317-327 ◽  
Author(s):  
Barbara Lies ◽  
Dieter Groneberg ◽  
Stepan Gambaryan ◽  
Andreas Friebe
Keyword(s):  
Guanylyl Cyclase ◽  
Cgmp Signalling

scholarly journals FRET-based cyclic GMP biosensors measure low cGMP concentrations in cardiomyocytes and neurons

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Gaia Calamera ◽  
Dan Li ◽  
Andrea Hembre Ulsund ◽  
Jeong Joo Kim ◽  
Oliver C. Neely ◽  
...  
Keyword(s):  
Guanylyl Cyclase ◽  
Intracellular Signaling ◽  
Stellate Ganglion ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Soluble Guanylyl Cyclase ◽  
High Affinity ◽  
Dependent Protein ◽  
Ganglion Neurons ◽  
Intracellular Detection

Abstract Several FRET (fluorescence resonance energy transfer)-based biosensors for intracellular detection of cyclic nucleotides have been designed in the past decade. However, few such biosensors are available for cGMP, and even fewer that detect low nanomolar cGMP concentrations. Our aim was to develop a FRET-based cGMP biosensor with high affinity for cGMP as a tool for intracellular signaling studies. We used the carboxyl-terminal cyclic nucleotide binding domain of Plasmodium falciparum cGMP-dependent protein kinase (PKG) flanked by different FRET pairs to generate two cGMP biosensors (Yellow PfPKG and Red PfPKG). Here, we report that these cGMP biosensors display high affinity for cGMP (EC50 of 23 ± 3 nM) and detect cGMP produced through soluble guanylyl cyclase and guanylyl cyclase A in stellate ganglion neurons and guanylyl cyclase B in cardiomyocytes. These biosensors are therefore optimal tools for real-time measurements of low concentrations of cGMP in living cells.

Morphological transformation of sensory ganglion neurons and satellite cells

2000 ◽  
Vol 11 (0) ◽  
pp. 39 ◽  
Author(s):  
Seiji Matsuda ◽  
Naoto Kobayashi ◽  
Hiroyuki Wakisaka ◽  
Shouichiro Saito ◽  
Kyouko Saito ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Sensory Ganglion ◽  
Morphological Transformation ◽  
Ganglion Neurons
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