scholarly journals An Innovative Technique For Extracorporeal Carbon Dioxide Removal Featuring An Electrodialysis Unit: An In-Vitro Experiment

2015 ◽  
Vol 3 (S1) ◽  
Author(s):  
A Zanella ◽  
D Ferlicca ◽  
S Abd El Aziz El Sayed Deab ◽  
S Colombo ◽  
S Spina ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Removal ◽  
Extracorporeal Carbon Dioxide Removal ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Innovative Technique

Carbon dioxide removal using low bicarbonate dialysis in rodents

Perfusion ◽  
2019 ◽  
Vol 34 (7) ◽  
pp. 578-583 ◽  
Author(s):  
Lien H Vu ◽  
John A Kellum ◽  
William J Federspiel ◽  
Matthew E Cove
Keyword(s):  
Carbon Dioxide ◽  
In Vitro Study ◽  
Blood Gases ◽  
Carbon Dioxide Removal ◽  
Low Flow ◽  
Sprague Dawley ◽  
Bicarbonate Concentration ◽  
Extracorporeal Carbon Dioxide Removal ◽  
Arterial Ph

Background: Extracorporeal carbon dioxide removal may be used to manage hypercapnia, but compared to dialysis, it’s not widely available. A recent in vitro study showed that dialysis with low bicarbonate dialysates removes CO2. Objective: To show that bicarbonate dialysis removes CO2 in an animal model to validate in-vitro findings and quantify the effect on arterial pH. Methods: Male Sprague-Dawley hypercapnic rats were dialyzed with either a conventional dialysate (PrismasolTM) or a bicarbonate-free dialysate (Bicarb0). The effect of dialysis on standard blood gases and electrolytes was measured. Results: Partial pressure of CO2 and bicarbonate concentration in blood decreased significantly after exposure to Bicarb0 compared to PrismasolTM (filter outflow values 12.8 vs 81.1 mmHg; p < 0.01 for CO2 and 3.5 vs 22.0 mmol/L; p < 0.01 for bicarbonate). Total CO2 content of blood was reduced by 459 mL/L during dialysis with Bicarb0 (filter inflow 546 ± 91 vs filter outflow 87 ± 52 mL/L; p < 0.01), but was not significantly reduced with PrismasolTM. Conclusions: Bicarbonate dialysis removes CO2 at rates comparable to existing low-flow ECCO2R.

scholarly journals Alkaline Liquid Ventilation of the Membrane Lung for Extracorporeal Carbon Dioxide Removal (ECCO2R): In Vitro Study

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 464
Author(s):  
Luigi Vivona ◽  
Michele Battistin ◽  
Eleonora Carlesso ◽  
Thomas Langer ◽  
Carlo Valsecchi ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
In Vitro Study ◽  
Carbon Dioxide Removal ◽  
Co2 Removal ◽  
Liquid Ventilation ◽  
Extracorporeal Carbon Dioxide Removal ◽  
Membrane Lung ◽  
Polypropylene Membrane ◽  
Removal Capacity

Extracorporeal carbon dioxide removal (ECCO2R) is a promising strategy to manage acute respiratory failure. We hypothesized that ECCO2R could be enhanced by ventilating the membrane lung with a sodium hydroxide (NaOH) solution with high CO2 absorbing capacity. A computed mathematical model was implemented to assess NaOH–CO2 interactions. Subsequently, we compared NaOH infusion, named “alkaline liquid ventilation”, to conventional oxygen sweeping flows. We built an extracorporeal circuit with two polypropylene membrane lungs, one to remove CO2 and the other to maintain a constant PCO2 (60 ± 2 mmHg). The circuit was primed with swine blood. Blood flow was 500 mL × min−1. After testing the safety and feasibility of increasing concentrations of aqueous NaOH (up to 100 mmol × L−1), the CO2 removal capacity of sweeping oxygen was compared to that of 100 mmol × L−1 NaOH. We performed six experiments to randomly test four sweep flows (100, 250, 500, 1000 mL × min−1) for each fluid plus 10 L × min−1 oxygen. Alkaline liquid ventilation proved to be feasible and safe. No damages or hemolysis were detected. NaOH showed higher CO2 removal capacity compared to oxygen for flows up to 1 L × min−1. However, the highest CO2 extraction power exerted by NaOH was comparable to that of 10 L × min−1 oxygen. Further studies with dedicated devices are required to exploit potential clinical applications of alkaline liquid ventilation.

In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

2019 ◽  
Vol 16 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Nasrin Zarkar ◽  
Mohammad Ali Nasiri Khalili ◽  
Fathollah Ahmadpour ◽  
Sirus Khodadadi ◽  
Mehdi Zeinoddini
Keyword(s):  
In Silico ◽  
Catalytic Domain ◽  
Md Simulations ◽  
Special Importance ◽  
Native Form ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Pharmaceutical Protein ◽  
The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

Effect of bleaching on ethyl glucuronide in hair: An in vitro experiment

2010 ◽  
Vol 198 (1-3) ◽  
pp. 23-27 ◽  
Author(s):  
Luca Morini ◽  
Alessandra Zucchella ◽  
Aldo Polettini ◽  
Lucia Politi ◽  
Angelo Groppi
Keyword(s):  
Ethyl Glucuronide ◽  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals A review of biocompatibility of zirconia: In vitro experiment

2018 ◽  
Vol 56 (4) ◽  
pp. 391 ◽  
Author(s):  
Da-Won Suh ◽  
Young-Kyun Kim ◽  
Yang-Jin Yi
Keyword(s):  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals Stratified Community Responses to Methane and Sulfate Supplies in Mud Volcano Deposits: Insights from an In Vitro Experiment

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113004 ◽  
Author(s):  
Yu Zhang ◽  
Lois Maignien ◽  
Alina Stadnitskaia ◽  
Pascal Boeckx ◽  
Xiang Xiao ◽  
...  
Keyword(s):  
Mud Volcano ◽  
Community Responses ◽  
Vitro Experiment ◽  
In Vitro Experiment
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