scholarly journals Genetic Effects of Soluble Starch Synthase IV-2 and It with ADPglucose Pyrophorylase Large Unit and Pullulanase on Rice Qualities

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Liang Xu ◽  
Hui You ◽  
Ouling Zhang ◽  
Xunchao Xiang
Keyword(s):  
Soluble Starch ◽  
Genetic Effects ◽  
Starch Synthase ◽  
Large Unit

scholarly journals Genetic Effects of Soluble Starch Synthase IV -2  and It with ADPglucose Pyrophorylase Large Unit  and Pullulanase  on Rice Qualities

2020 ◽  
Author(s):  
Liang Xu ◽  
Hui You ◽  
Ouling Zhang ◽  
Xunchao Xiang
Keyword(s):  
Amylose Content ◽  
Soluble Starch ◽  
Genetic Effects ◽  
Starch Synthase ◽  
Rice Starch ◽  
Peak Time ◽  
Major Genes ◽  
Rice Quality ◽  
Large Unit ◽  
Apparent Amylose Content

Abstract BackgroundRice amylose content and amylopectin structure corporately determine rice eating and cooking qualities (ECQs). Soluble starch synthase IV-2 (SSIV-2) is a member of the soluble starch synthesis gene family but with unknown effects on ECQs.Results In this study, three populations derived from a cross of two parents who possess the same major genes of starch bio-synthesis were employed to investigate the influence of SSIV-2 and its combined effects with ADPglucose pyrophorylase large unit (AGPlar) and Pullulanase (PUL) on ECQs. The results illustrated that the polymorphism of SSIV-2 alleles significantly affected gel consistency (GC), gelatinization temperature (GT), percent of retrogradation (R) and three crucial RVA profile parameters: peak viscosity (PKV), breakdown viscosity (BDV) and setback viscosity (SBV). And SSIV-2 allele derived from CG173R had better quality traits with lower GT, SBV and R. Moreover, its interaction with AGPlar was responsible for the variations of GC, apparent amylose content (AAC), GT, R and all RVA parameters except for pasting temperature (PaT) and peak time (PeT), in terms of GC, PKV and CSV, AGPlar derived from CG173R had an epistasis effect on SSIV-2; additionally, interaction of SSIV-2 and PUL mainly affected GC, AAC, PKV, CPV, CSV and SBV. I-C and C-1 (I, allele of AGPlar from Guangzhan 63S; C, allele of SSIV-2 from CG173R; 1, allele of PUL from Guangzhan 63S) combinations had better ECQs. The findings suggest that genetic effects of SSIV-2 and its interaction with AGPlar and PUL are vital for rice quality under the same major genes (Wx and SSII-3) background. ConclusionsSS IV-2 significantly affect GC, R, GT, PKV, BDV and SBV of rice starch. The I-C and C-1 have better ECQs. These findings suggest that the effects of SS IV-2 and its interaction with AGPlar and PUL are vital for rice quality breeding.

scholarly journals Genetic Effects of Soluble Starch Synthase IV-2 and It with ADPglucose Pyrophorylase Large Unit and Pullulanase on Rice Qualities

2020 ◽  
Author(s):  
Liang Xu ◽  
Hui You ◽  
Ouling Zhang ◽  
Xunchao Xiang
Keyword(s):  
Amylose Content ◽  
Starch Synthesis ◽  
Thermal Characteristics ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Peak Time ◽  
Major Genes ◽  
Rice Quality ◽  
Large Unit ◽  
Apparent Amylose Content

Abstract Background: Rice amylose content and amylopectin structure corporately determine rice eating and cooking qualities (ECQs). Soluble starch synthase ( SS ) IV-2 is a member of the soluble starch synthesis gene family but with unknown effects on ECQs. Results: In this study, three populations derived from a cross of two parents who possess the same major genes of starch bio-synthesis were employed to investigate the influence of SSIV-2 and its combined effects with ADPglucose pyrophorylase large unit ( AGPlar ) and Pullulanase ( PUL ) on ECQs. The results illustrated that the polymorphism of SSIV-2 alleles significantly affected gel consistency (GC), gelatinization temperature (GT), percent of retrogradation (PR) and three crucial rapid viscosity analysis (RVA) profile parameters: peak viscosity (PKV), breakdown viscosity (BDV) and setback viscosity (SBV). And SSIV-2 allele derived from CG173R had better quality traits with lower GT, SBV and PR. Moreover, its interaction with AGPlar was responsible for the variations of GC, apparent amylose content (AAC), GT, PR and all RVA parameters except for pasting temperature (PaT) and peak time (PeT), in terms of GC, PKV and CSV, AGPlar derived from CG173R had an epistatic effect on SSIV-2 ; additionally, interaction of SSIV-2 and PUL mainly affected GC, AAC, PKV, CPV, CSV and SBV. I-C and C-1 (I, allele of AGPlar from Guangzhan 63S; C, allele of SSIV-2 from CG173R; 1, allele of PUL from Guangzhan 63S) combinations had better ECQs. Conclusions: SSIV-2 alleles significantly affect rice quality, especially the parameters relevant to gelatinized and thermal characteristics of starch (GC, PR, GT, PKV, BDV and SBV) under the same major genes ( Waxy and SSII-3 ) background. It indicates that SSIV-2 functions elongation of starch chain. These findings suggest that the effects of SSIV-2 and its interaction with AGPlar and PUL are vital for rice quality breeding with the same major genes.

scholarly journals Toward an understanding of the biogenesis of the starch granule. Determination of granule-bound and soluble starch synthase functions in amylopectin synthesis.

1994 ◽  
Vol 269 (40) ◽  
pp. 25150-25157
Author(s):  
M.L. Maddelein ◽  
N. Libessart ◽  
F. Bellanger ◽  
B. Delrue ◽  
C. D'Hulst ◽  
...  
Keyword(s):  
Starch Granule ◽  
Soluble Starch ◽  
Starch Synthase

scholarly journals QTL-seq identifies cooked grain elongation QTLs near soluble starch synthase and starch branching enzymes in rice (Oryza sativa L.)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Siwaret Arikit ◽  
Samart Wanchana ◽  
Srisawat Khanthong ◽  
Chatree Saensuk ◽  
Tripop Thianthavon ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Starch Branching Enzymes ◽  
Branching Enzymes

scholarly journals Structure, Organization, and Chromosomal Location of the Gene Encoding a Form of Rice Soluble Starch Synthase

1995 ◽  
Vol 108 (2) ◽  
pp. 677-683 ◽  
Author(s):  
Ki. Tanaka ◽  
S. Ohnishi ◽  
N. Kishimoto ◽  
T. Kawasaki ◽  
T. Baba
Keyword(s):  
Chromosomal Location ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Gene Encoding

scholarly journals Expression of a rice soluble starch synthase gene in transgenic wheat improves the grain yield under heat stress conditions

2018 ◽  
Vol 54 (3) ◽  
pp. 216-227 ◽  
Author(s):  
Bin Tian ◽  
Shyamal K. Talukder ◽  
Jianming Fu ◽  
Allan K. Fritz ◽  
Harold N. Trick
Keyword(s):  
Heat Stress ◽  
Grain Yield ◽  
Transgenic Wheat ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Stress Conditions

Thermal Characteristics of Soluble Starch Synthase From Wheat Endosperm

1995 ◽  
Vol 22 (4) ◽  
pp. 703 ◽  
Author(s):  
CF Jenner ◽  
K Denyer ◽  
J Guerin
Keyword(s):  
Thermal Characteristics ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Wheat Grain ◽  
Wheat Endosperm ◽  
Temperature Range ◽  
Higher Temperature ◽  
Temperature Responses

The aim of the work reported in this paper was to characterise the thermal responses of soluble starch synthase (SSS) extracted from the endosperm of the developing wheat grain. Using partially purified preparations of SSS, the reaction obeyed Michaelis-Menten kinetics with both substrates amylopectin and ADPglucose. Both the Vmax and the Km varied with temperature. Values for Vmax were higher at 45�C compared with 25�C. However, the Km values for both substrates were also higher at 45�C than at 25�C indicating that the affinity of the enzyme for its substrates was reduced at the higher temperature. Over the temperature range 15-45�C, the Km for arnylopectin was minimal at 20�C, and rose exponentially between 25 and 45�C. Kinetic analyses indicated that the reaction was sequential and that the substrates could bind to the enzyme in either order. At 25�C the binding of one substrate to the enzyme increased the affinity of the complex for the second substrate but at 45�C these effects were abolished. These thermal characteristics of SSS could explain certain important features of the temperature responses of starch deposition in the wheat grain in vivo.

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