scholarly journals Piglet nasal microbiota at weaning may influence the development of Glässer’s disease during the rearing period

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Florencia Correa-Fiz ◽  
Lorenzo Fraile ◽  
Virginia Aragon
Keyword(s):  
Glässer’S Disease ◽  
Glasser's Disease ◽  
Nasal Microbiota

scholarly journals Altered Nasal Microbiota Composition Associated with Development of Polyserositis by Mycoplasma hyorhinis

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 603
Author(s):  
Miguel Blanco-Fuertes ◽  
Florencia Correa-Fiz ◽  
Lorenzo Fraile ◽  
Marina Sibila ◽  
Virginia Aragon
Keyword(s):  
Pathological Finding ◽  
Systemic Infection ◽  
Amplicon Sequencing ◽  
Rrna Gene ◽  
Upper Respiratory Tract ◽  
Mycoplasma Hyorhinis ◽  
Predisposing Factor ◽  
Glässer’S Disease ◽  
Glasser's Disease ◽  
Nasal Microbiota

Fibrinous polyserositis in swine farming is a common pathological finding in nursery animals. The differential diagnosis of this finding should include Glaesserella parasuis (aetiological agent of Glässer’s disease) and Mycoplasma hyorhinis, among others. These microorganisms are early colonizers of the upper respiratory tract of piglets. The composition of the nasal microbiota at weaning was shown to constitute a predisposing factor for the development of Glässer’s disease. Here, we unravel the role of the nasal microbiota in the subsequent systemic infection by M. hyorhinis, and the similarities and differences with Glässer’s disease. Nasal samples from farms with recurrent problems with polyserositis associated with M. hyorhinis (MH) or Glässer’s disease (GD) were included in this study, together with healthy control farms (HC). Nasal swabs were taken from piglets in MH farms at weaning, before the onset of the clinical outbreaks, and were submitted to 16S rRNA gene amplicon sequencing (V3–V4 region). These sequences were analyzed together with sequences from similar samples previously obtained in GD and HC farms. Animals from farms with disease (MH and GD) had a nasal microbiota with lower diversity than those from the HC farms. However, the composition of the nasal microbiota of the piglets from these disease farms was different, suggesting that divergent microbiota imbalances may predispose the animals to the two systemic infections. We also found variants of the pathogens that were associated with the farms with the corresponding disease, highlighting the importance of studying the microbiome at strain-level resolution.

scholarly journals Outbreak of peracute Glässer’s disease in suckling piglets in a self-recruiting high health farrow to grower herd: A Case report

2020 ◽  
Author(s):  
Odd Magne Karlsen
Keyword(s):  
Room Temperature ◽  
Management Practices ◽  
Histopathological Examination ◽  
Bacteriological Examination ◽  
Case Presentation ◽  
Procaine Penicillin ◽  
Glässer’S Disease ◽  
Suckling Piglets ◽  
Poor Ventilation ◽  
Glasser's Disease

Abstract BackgroundGlässer’s disease is caused by a bacterial infection where the causative agent is Glaesserella parasuis and is of rising concern in Norwegian high health pig herds. Different factors can trigger the onset of Glässer’s disease, including management practices such as unstable room temperature, poor ventilation or impaired immunity. Outbreaks of peracute Glässer’s disease in suckling piglets are rarely described. Case presentationIn a high health, farrow to grower pig herd 270 suckling piglets out of 920 died in a peracute to acute manner during a 20-day period. Post-mortem examinations revealed findings consistent with congestion and oedema and histopathological examination found no signs of inflammation or intravascular microthrombi. Bacteriological examination detected G. parasuis in relevant tissues. Emesis in half of the piglets and central nervous signs in a few was found after first intramuscular injection of procaine penicillin. Anaemia, poor temperature regulation in the farrowing unit and poor hygiene were factors contributing to the disease outbreak.ConclusionsIn the present case, it was concluded that an outbreak with high mortality and predominantly peracute to acute clinical disease in 3-5 week old suckling piglets was caused by Glässer’s disease (G. parasuis).

scholarly journals Protective Efficacy of an Inactive Vaccine Based on the LY02 Isolate against AcuteHaemophilus parasuisInfection in Piglets

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiao-Hua Li ◽  
Guo-Zhen Zhao ◽  
Long-Xin Qiu ◽  
Ai-Ling Dai ◽  
Wang-Wei Wu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Protective Immunity ◽  
Protective Efficacy ◽  
Haemophilus Parasuis ◽  
Fujian Province ◽  
Cellular Immune Responses ◽  
Glässer’S Disease ◽  
Potential Vaccine ◽  
Cellular Immune ◽  
Glasser's Disease

Haemophilus parasuiscan cause Glässer’s disease characterized by fibrinous polyserositis, polyarthritis, and meningitis. The current prevention of Glässer’s disease is mainly based on the inactive vaccines; however, the protective efficacy usually fails in heterogeneous or homologous challenges. Here, the predominant lineage ofH. parasuis(LY02 strain) in Fujian province, China, characterized as serovar 5, was used to evaluate the protective immunity against acuteH. parasuisinfection in piglets after inactivation. Following challenging withH. parasuis,only mild lesions in the pigs immunized with the killed vaccine were observed, whereas the typical symptoms of Glässer’s disease presented in the nonimmunized piglets. A strong IgG immune response was induced by the inactive vaccine. CD4+and CD8+T lymphocyte levels were increased, indicating the potent cellular immune responses were elicited. The significantly high levels of IL-2, IL-4, TGF-β, and IFN-γin sera from pigs immunized with this killed vaccine suggested that the mixed Th1 and Th2 immune responses were induced, associated with the high protection againstH. parasuisinfection compared to the nonimmunized animals. This study indicated that the inactivated LY02 strain ofH. parasuiscould serve as a potential vaccine candidate to prevent the prevalence ofH. parasuisin Fujian province, China.

GLASSER'S DISEASE OF SWINE

1947 ◽  
Vol 23 (4) ◽  
pp. 91-94 ◽  
Author(s):  
A. K. Sutherland ◽  
G. C. Simmons
Keyword(s):  
Glässer’S Disease ◽  
Glasser's Disease

scholarly journals Update on Glässer’s disease: How to control the disease under restrictive use of antimicrobials

2020 ◽  
Vol 242 ◽  
pp. 108595 ◽  
Author(s):  
Mar Costa-Hurtado ◽  
Emili Barba-Vidal ◽  
Jaime Maldonado ◽  
Virginia Aragon
Keyword(s):  
Glässer’S Disease ◽  
Glasser's Disease

Effect of Different Vaccine Formulations on the Development of Glässer's Disease Induced in Pigs by Experimental Haemophilus parasuis Infection

2009 ◽  
Vol 140 (2-3) ◽  
pp. 169-176 ◽  
Author(s):  
A.J. Martín de la Fuente ◽  
C.B. Gutiérrez Martín ◽  
C. Pérez Martínez ◽  
M.J. García Iglesias ◽  
F. Tejerina ◽  
...  
Keyword(s):  
Haemophilus Parasuis ◽  
Glässer’S Disease ◽  
Glasser's Disease

Corrigendum to “Dynamics of Haemophilus parasuis genotypes in a farm recovered from an outbreak of Glässer's disease” [Vet. Microbiol. 123 (2007) 230–237]

2012 ◽  
Vol 157 (1-2) ◽  
pp. 251
Author(s):  
A. Olvera ◽  
M. Cerdà-Cuèllar ◽  
M. Nofrarías ◽  
E. Revilla ◽  
J. Segalès ◽  
...  
Keyword(s):  
Haemophilus Parasuis ◽  
Glässer’S Disease ◽  
Glasser's Disease

scholarly journals Detection of Haemophilus parasuis isolates from South China by loop-mediated isothermal amplification and isolate characterisation

2012 ◽  
Vol 79 (1) ◽  
Author(s):  
Jian-min Zhang ◽  
Hai-yan Shen ◽  
Ming Liao ◽  
Tao Ren ◽  
Li-li Guo ◽  
...  
Keyword(s):  
16S Rrna ◽  
South China ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Economic Losses ◽  
Lamp Method ◽  
Haemophilus Parasuis ◽  
Loop Mediated Isothermal Amplification ◽  
Glässer’S Disease ◽  
Glasser's Disease

Haemophilus parasuis is the etiological agent of Glässer’s disease, which is characterised by fibrinous polyserositis, meningitis and polyarthritis, causing severe economic losses to the swine industry. In this study, a loop-mediated isothermal amplification (LAMP) test was developed to improve the specificity, facility and speed of diagnosis of H. parasuis isolates. The LAMP assay rapidly amplified the target gene within 50 min incubation at 63 °C in a laboratory water bath. The LAMP amplicon could be visualised directly in the reaction tubes following the addition of SYBR Green I dye. The detection limit of this LAMP method was 10 CFU/mL, which was 10 times more sensitive than the earlier 16S rRNA polymerase chain reaction (PCR) test conducted by Oliveira, Galina and Pijoan (2001), and no cross-reactivity was observed from other non-H. parasuis strains. This LAMP test was evaluated further on 187 clinical specimens from pigs suspected of being infected with H. parasuis. Forty-three were found positive by bacterial isolation of H. parasuis, as well as by the 16S rRNA PCR and LAMP tests. The 43 H. parasuis isolates were classified into 9 serovars and had 37 genetic patterns when analysed by pulsed-field gel electrophoresis (PFGE). This displayed that various H. parasuis serovars and genotypes were widely distributed in South China. Therefore, the speed, specificity and sensitivity of the LAMP test, the lack of a need for expensive equipment, and the visual readout showed great potential for a correct clinical diagnosis of H. parasuis in favour of controlling Glässer’s disease.

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