Dissection of the multigenic wheat stem rust resistance present in the Montenegrin spring wheat accession PI 362698

Adult plant resistance (APR) to wheat stem rust has been one of the approaches for resistance breeding since the evolution of the Ug99 race group and other races. This study was conducted to dissect and understand the genetic basis of APR to stem rust in spring wheat line ‘Copio’. A total of 176 recombinant inbred lines (RIL) from the cross of susceptible parent ‘Apav’ with Copio were phenotyped for stem rust resistance in six environments. Composite interval mapping (CIM) using 762 Genotyping-by-Sequencing (GBS) markers, identified 16 genomic regions conferring stem rust resistance. Assays with gene-linked molecular markers revealed that Copio carried known APR genes Sr2 and Lr46/Yr29/Sr58 in addition to the 2NS/2AS translocation that harbors race-specific genes Sr38, Lr37 and Yr17. Three QTL were mapped on chromosomes 2B, two QTL on chromosomes 3A, 3B, and 6A each, and one QTL on each of chromosomes 2A, 1B, 2D, 4B, 5D, 6D and 7A. The QTL QSr.umn.5D is potentially a new resistance gene and contributed to quantitative resistance in Copio. The RILs with allelic combinations of Sr2, Sr38, and Sr58 had 27-39% less stem rust coefficient of infection in all field environments compared to RILs with none of these genes and this gene combination was most effective in the US environments. We conclude that Copio carries several genes that provide both race-specific and non-race-specific resistance to diverse races of stem rust fungus and can be used by breeding programs in pyramiding other effective genes to develop durable resistance in wheat.

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THE INHERITANCE OF PATHOGENICITY IN RACES 111 AND 29 OF WHEAT STEM RUST

Canadian Journal of Genetics and Cytology ◽

10.1139/g69-033 ◽

1969 ◽

Vol 11 (2) ◽

pp. 266-274 ◽

Cited By ~ 4

Author(s):

K. N. Kao ◽

D. R. Knott

Keyword(s):

Stem Rust ◽

Rust Resistance ◽

Single Gene ◽

Stem Rust Resistance ◽

Wheat Stem Rust ◽

Complementary Genes

The inheritance of pathogenicity in wheat stem rust was studied in selfed cultures of races 29 and 111, F1 and F2 cultures of a cross between the two races and cultures from a backcross to race 29. The various cultures were tested on Marquis and Prelude and on a series of lines of these varieties carrying single genes for stem rust resistance. Virulence on Sr 5, Sr 6, Sr 8, Sr 9a, Sr 14 and a gene in Marquis was recessive and in each case there was a single gene for virulence corresponding to each gene for resistance. Virulence on Sr 1 was possibly controlled by two dominant complementary genes. There appeared to be two alleles for virulence on Prelude, one dominant and one recessive.

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Stem Rust Resistance in A-Genome Diploid Relatives of Wheat

Plant Disease ◽

10.1094/pdis-04-10-0260 ◽

2011 ◽

Vol 95 (8) ◽

pp. 941-944 ◽

Cited By ~ 39

Author(s):

M. N. Rouse ◽

Y. Jin

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Infection Type ◽

Genetic Resistance ◽

Wheat Breeding ◽

Stem Rust Resistance ◽

Wheat Stem Rust ◽

A Genome ◽

Stem Rust Resistance Genes

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, has been effectively controlled through the use of genetic resistance. P. graminis f. sp. tritici race TTKSK (Ug99) possesses virulence to many resistance genes that have been used in wheat breeding worldwide. One strategy to aid breeders in developing resistant cultivars is to utilize resistance genes transferred from wild relatives to wheat. Stem rust resistance genes have previously been introgressed from Triticum monococcum to wheat. In order to identify additional resistance genes, we screened 1,061 accessions of T. monococcum and 205 accessions of T. urartu against race TTKSK and four additional P. graminis f. sp. tritici races: TTTTF, TRTTF, QFCSC, and MCCFC. A high frequency of the accessions (78.7% of T. monococcum and 93.0% of T. urartu) were resistant to P. graminis f. sp. tritici race TTKSK, with infection types ranging from 0 to 2+. Among these resistant accessions, 55 T. monococcum accessions (6.4% of the total) were also resistant to the other four races. Associations of resistance in T. monococcum germplasm to different races indicated the presence of genes conferring resistance to multiple races. Comparing the observed infection type patterns to the expected patterns of known genes indicated that previously uncharacterized genes for resistance to race TTKSK exist in both T. monococcum and T. urartu.

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Identification and characterization of Sr22b , a new allele of the wheat stem rust resistance gene Sr22 effective against the Ug99 race group

Plant Biotechnology Journal ◽

10.1111/pbi.13737 ◽

2021 ◽

Author(s):

Jing Luo ◽

Matthew N. Rouse ◽

Lei Hua ◽

Hongna Li ◽

Boshu Li ◽

...

Keyword(s):

Resistance Gene ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Rust Resistance Gene ◽

Stem Rust Resistance Gene ◽

Wheat Stem Rust ◽

Identification And Characterization

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Mapping and characterization of wheat stem rust resistance genes SrTm5 and Sr60 from Triticum monococcum

Theoretical and Applied Genetics ◽

10.1007/s00122-017-3024-z ◽

2017 ◽

Vol 131 (3) ◽

pp. 625-635 ◽

Cited By ~ 10

Author(s):

Shisheng Chen ◽

Yan Guo ◽

Jordan Briggs ◽

Felix Dubach ◽

Shiaoman Chao ◽

...

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Triticum Monococcum ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

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Identification of stem rust resistance genes in wheat cultivars in China using molecular markers

PeerJ ◽

10.7717/peerj.4882 ◽

2018 ◽

Vol 6 ◽

pp. e4882 ◽

Cited By ~ 7

Author(s):

Xiaofeng Xu ◽

Depeng Yuan ◽

Dandan Li ◽

Yue Gao ◽

Ziyuan Wang ◽

...

Keyword(s):

Molecular Markers ◽

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Genetic Material ◽

Stem Rust Resistance ◽

Wheat Cultivars ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

Wheat stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn. (Pgt), is a major disease that has been effectively controlled using resistance genes. The appearance and spread of Pgt races such as Ug99, TKTTF, and TTTTF, which are virulent to most stem rust-resistant genes currently deployed in wheat breeding programs, renewed the interest in breeding cultivars resistant to wheat stem rust. It is therefore important to investigate the levels of resistance or vulnerability of wheat cultivars to Pgt races. Resistance to Pgt races 21C3CTHQM, 34MKGQM, and 34C3RTGQM was evaluated in 136 Chinese wheat cultivars at the seedling stage. A total of 124 cultivars (91.2%) were resistant to the three races. Resistance genes Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38 were analyzed using molecular markers closely linked to them, and 63 of the 136 wheat cultivars carried at least one of these genes: 21, 25, and 28 wheat cultivars likely carried Sr2, Sr31, and Sr38, respectively. Cultivars “Kehan 3” and “Jimai 22” likely carried Sr25. None of the cultivars carried Sr24 or Sr26. These cultivars with known stem rust resistance genes provide valuable genetic material for breeding resistant wheat cultivars.

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