Transcriptome profiling analysis for two Tibetan wild barley genotypes in responses to low nitrogen

Keeping the significance of potassium (K) nutrition in focus, this study explores the genotypic responses of two wild Tibetan barley genotypes (drought tolerant XZ5 and drought sensitive XZ54) and one drought tolerant barley cv. Tadmor, under the exposure of polyethylene glycol-induced drought stress. The results revealed that drought and K deprivation attenuated overall plant growth in all the tested genotypes; however, XZ5 was least affected due to its ability to retain K in its tissues which could be attributed to the smallest reductions of photosynthetic parameters, relative chlorophyll contents and the lowest Na+/K+ ratios in all treatments. Our results also indicate that higher H+/K+-ATPase activity (enhancement of 1.6 and 1.3-fold for shoot; 1.4 and 2.5-fold for root), higher shoot K+ (2 and 2.3-fold) and Ca2+ content (1.5 and 1.7-fold), better maintenance of turgor pressure by osmolyte accumulation and enhanced antioxidative performance to scavenge ROS, ultimately suppress lipid peroxidation (in shoots: 4% and 35%; in roots 4% and 20% less) and bestow higher tolerance to XZ5 against drought stress in comparison with Tadmor and XZ54, respectively. Conclusively, this study adds further evidence to support the concept that Tibetan wild barley genotypes that utilize K efficiently could serve as a valuable genetic resource for the provision of genes for improved K metabolism in addition to those for combating drought stress, thereby enabling the development of elite barley lines better tolerant of abiotic stresses.

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Response of Tibetan Wild Barley Genotypes to Drought Stress and Identification of Quantitative Trait Loci by Genome-Wide Association Analysis

International Journal of Molecular Sciences ◽

10.3390/ijms20030791 ◽

2019 ◽

Vol 20 (3) ◽

pp. 791

Author(s):

Mian Zhang ◽

Man-Man Fu ◽

Cheng-Wei Qiu ◽

Fangbin Cao ◽

Zhong-Hua Chen ◽

...

Keyword(s):

Genetic Variation ◽

Drought Stress ◽

Drought Tolerance ◽

Quantitative Trait ◽

Wild Barley ◽

Genome Wide Association ◽

Dart Markers ◽

Tibetan Wild Barley ◽

Genome Wide ◽

Barley Genotypes

Tibetan wild barley has been identified to show large genetic variation and stress tolerance. A genome-wide association (GWA) analysis was performed to detect quantitative trait loci (QTLs) for drought tolerance using 777 Diversity Array Technology (DArT) markers and morphological and physiological traits of 166 Tibetan wild barley accessions in both hydroponic and pot experiments. Large genotypic variation for these traits was found; and population structure and kinship analysis identified three subpopulations among these barley genotypes. The average LD (linkage disequilibrium) decay distance was 5.16 cM, with the minimum on 6H (0.03 cM) and the maximum on 4H (23.48 cM). A total of 91 DArT markers were identified to be associated with drought tolerance-related traits, with 33, 26, 16, 1, 3, and 12 associations for morphological traits, H+K+-ATPase activity, antioxidant enzyme activities, malondialdehyde (MDA) content, soluble protein content, and potassium concentration, respectively. Furthermore, 7 and 24 putative candidate genes were identified based on the reference Meta-QTL map and by searching the Barleymap. The present study implicated that Tibetan annual wild barley from Qinghai–Tibet Plateau is rich in genetic variation for drought stress. The QTLs detected by genome-wide association analysis could be used in marker-assisting breeding for drought-tolerant barley genotypes and provide useful information for discovery and functional analysis of key genes in the future.

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Metabolic analysis of two contrasting wild barley genotypes grown hydroponically reveals adaptive strategies in response to low nitrogen stress

Journal of Plant Physiology ◽

10.1016/j.jplph.2016.07.020 ◽

2016 ◽

Vol 206 ◽

pp. 59-67 ◽

Cited By ~ 15

Author(s):

Xiaoyan Quan ◽

Qiufeng Qian ◽

Zhilan Ye ◽

Jianbin Zeng ◽

Zhigang Han ◽

...

Keyword(s):

Wild Barley ◽

Adaptive Strategies ◽

Nitrogen Stress ◽

Metabolic Analysis ◽

Low Nitrogen Stress ◽

Low Nitrogen ◽

Barley Genotypes

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Transcriptome profiling analysis of two contrasting barley genotypes in general combining ability for yield traits

Revista Brasileira de Botânica ◽

10.1007/s40415-020-00696-3 ◽

2021 ◽

Author(s):

Xiaodong Chen ◽

Bin Zhao ◽

Changhao Ji ◽

Bin Zhu ◽

Rui Wang

Keyword(s):

Combining Ability ◽

Transcriptome Profiling ◽

General Combining Ability ◽

Yield Traits ◽

Profiling Analysis ◽

Barley Genotypes

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Single Nucleotide Polymorphisms in Bmy1 Intron III Alleles Conferring the Genotypic Variation in β-amylase Activity under Drought Stress Between Tibetan Wild and Cultivated Barley

10.21203/rs.3.rs-790936/v1 ◽

2021 ◽

Author(s):

Xiaojian Wu ◽

Huan Wang ◽

Fanrong Zeng ◽

Junmei Wang

Keyword(s):

Amino Acid ◽

Drought Stress ◽

Wild Barley ◽

Amylase Activity ◽

Genotypic Variation ◽

Nucleotide Polymorphisms ◽

Unique Amino Acid Substitution ◽

Tibetan Wild Barley ◽

Cultivated Barley ◽

Barley Genotypes

Abstract β-amylase activity is related to the polymorphism of Bmy1 intron III; however, no attention has been given to such relationship under environmental stresses like drought. In this study, 73 cultivated barley genotypes and 52 Tibetan wild barley accessions were used to test the association between Bmy1 gene intron III polymorphisms and β-amylase activity under drought stress. Our results showed that three alleles, Bmy1.a, Bmy1.b and Bmy1.c, existed in the examined barley genotypes. Tibetan wild barley had higher proportion of Bmy1.b, whereas cultivated barley showed higher proportion of Bmy1.a. Impressively, barley genotypes with Bmy1.b showed significant increase in β-amylase activity under drought stress, compared with those with Bmy1.a or Bmy1.c, indicating that Bmy1.b allele might provide more chances for developing barley cultivars with higher β-amylase activity under water stress than both Bmy1.a and Bmy1.c alleles. Furthermore, the Tibetan wild barley XZ147, belonging to Bmy1.b allele type, showed significant higher β-amylase activity than the cultivar Triumph under drought stress. This might result from the unique amino acid substitution M527 or the amino acid composition of R115, D165, A233, S347 and M527 of XZ147.

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Genome-Wide Identification and Characterization of Drought Stress Responsive microRNAs in Tibetan Wild Barley

International Journal of Molecular Sciences ◽

10.3390/ijms21082795 ◽

2020 ◽

Vol 21 (8) ◽

pp. 2795

Author(s):

Cheng-Wei Qiu ◽

Li Liu ◽

Xue Feng ◽

Peng-Fei Hao ◽

Xiaoyan He ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Target Genes ◽

Wild Barley ◽

Expression Profiles ◽

Tibetan Wild Barley ◽

Drought Tolerant ◽

Genome Wide ◽

Barley Genotypes

Drought stress is a major obstacle to agricultural production. Tibetan wild barley with rich genetic diversity is useful for drought-tolerant improvement of cereals. MicroRNAs (miRNAs) play critical roles in controlling gene expression in response to various environment perturbations in plants. However, the genome-wide expression profiles of miRNAs and their targets in response to drought stress are largely unknown in wild barley. In this study, a polyethylene glycol (PEG) induced drought stress hydroponic experiment was performed, and the expression profiles of miRNAs from the roots of two contrasting Tibetan wild barley genotypes XZ5 (drought-tolerant) and XZ54 (drought-sensitive), and one cultivated barley Tadmor (drought-tolerant) generated by high-throughput sequencing were compared. There were 69 conserved miRNAs and 1574 novel miRNAs in the dataset of three genotypes under control and drought conditions. Among them, seven conserved miRNAs and 36 novel miRNAs showed significantly genotype-specific expression patterns in response to drought stress. And 12 miRNAs were further regarded as drought tolerant associated miRNAs in XZ5, which mostly participate in gene expression, metabolism, signaling and transportation, suggesting that they and their target genes play important roles in plant drought tolerance. This is the first comparation study on the miRNA transcriptome in the roots of two Tibetan wild barley genotypes differing in drought tolerance and one drought tolerant cultivar in response to PEG treatment. Further results revealed the candidate drought tolerant miRNAs and target genes in the miRNA regulation mechanism in wild barley under drought stress. Our findings provide valuable understandings for the functional characterization of miRNAs in drought tolerance.

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