scholarly journals Identification of cold stress responsive microRNAs in two winter turnip rape (Brassica rapa L.) by high throughput sequencing

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Xiucun Zeng ◽  
Yaozhao Xu ◽  
Jinjin Jiang ◽  
Fenqin Zhang ◽  
Li Ma ◽  
...  
Keyword(s):  
Cold Stress ◽  
Brassica Rapa ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Turnip Rape

scholarly journals The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra

Genomics Data ◽  
2015 ◽  
Vol 3 ◽  
pp. 1-3 ◽  
Author(s):  
Muhammad Awais Ghani ◽  
Junxing Li ◽  
Linli Rao ◽  
Muhammad Ammar Raza ◽  
Liwen Cao ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Throughput ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Brassica Nigra ◽  
Wide Hybridisation

scholarly journals iTRAQ-Based Quantitative Proteome Revealed Metabolic Changes in Winter Turnip Rape (Brassica rapa L.) under Cold Stress

2018 ◽  
Vol 19 (11) ◽  
pp. 3346 ◽  
Author(s):  
Yaozhao Xu ◽  
Xiucun Zeng ◽  
Jian Wu ◽  
Fenqin Zhang ◽  
Caixia Li ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Cold Stress ◽  
Brassica Rapa ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Enrichment Analysis ◽  
Pentose Phosphate ◽  
Metabolic Process ◽  
Cellular Process ◽  
Turnip Rape

Winter turnip rape (Brassica rapa L.) is a large-scale winter-only oil crop cultivated in Northwest China. However, its cold-resistant molecular mechanism remains inadequate. Studying the cold adaptation mechanisms of winter turnip rape based on the proteomic technique of isobaric tags for relative and absolute quantification (iTRAQ) offers a solution to this problem. Under cold stress (−4 °C for eight hours), 51 and 94 differently accumulated proteins (DAPs) in Longyou 7 (cold-tolerant) and Tianyou 4 (cold-sensitive) were identified, respectively. These DAPs were classified into 38 gene ontology (GO) term categories, such as metabolic process, cellular process, catalytic activity, and binding. The 142 DAPs identified between the two cold-stressed cultivars were classified into 40 GO terms, including cellular process, metabolic process, cell, catalytic activity, and binding. Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DAPs participated in 10 pathways. The abundance of most protein functions in ribosomes, carbon metabolism, photosynthesis, and energy metabolism including the citrate cycle, pentose phosphate pathway, and glyoxylate and dicarboxylate metabolism decreased, and the proteins that participate in photosynthesis–antenna and isoflavonoid biosynthesis increased in cold-stressed Longyou 7 compared with those in cold-stressed Tianyou 4. The expression pattern of genes encoding the 10 significant DAPs was consistent with the iTRAQ data. This study provides new information on the proteomic differences between the leaves of Longyou 7 and Tianyou 4 plants and explains the possible molecular mechanisms of cold-stress adaptation in B. rapa.

High-throughput sequencing discovery of conserved and novel microRNAs in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

2012 ◽  
Vol 287 (7) ◽  
pp. 555-563 ◽  
Author(s):  
Fengde Wang ◽  
Libin Li ◽  
Lifeng Liu ◽  
Huayin Li ◽  
Yihui Zhang ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Throughput ◽  
Chinese Cabbage ◽  
High Throughput Sequencing

scholarly journals Identification of differentially expressed genes involved in amino acid and lipid accumulation of winter turnip rape (Brassica rapa L.) in response to cold stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0245494
Author(s):  
Yan Fang ◽  
Jeffrey A. Coulter ◽  
Junyan Wu ◽  
Lijun Liu ◽  
Xuecai Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Brassica Rapa ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Differentially Expressed ◽  
Serine Acetyltransferase ◽  
Turnip Rape

Winter turnip rape (Brassica rapa L.) is an important overwintering oil crop that is widely planted in northwestern China. It considered to be a good genetic resource for cold-tolerant research because its roots can survive harsh winter conditions. Here, we performed comparative transcriptomics analysis of the roots of two winter turnip rape varieties, Longyou7 (L7, strong cold tolerance) and Tianyou2 (T2, low cold tolerance), under normal condition (CK) and cold stress (CT) condition. A total of 8,366 differentially expressed genes (DEGs) were detected between the two L7 root groups (L7CK_VS_L7CT), and 8,106 DEGs were detected for T2CK_VS_T2CT. Among the DEGs, two ω-3 fatty acid desaturase (FAD3), two delta-9 acyl-lipid desaturase 2 (ADS2), one diacylglycerol kinase (DGK), and one 3-ketoacyl-CoA synthase 2 (KCS2) were differentially expressed in the two varieties and identified to be related to fatty acid synthesis. Four glutamine synthetase cytosolic isozymes (GLN), serine acetyltransferase 1 (SAT1), and serine acetyltransferase 3 (SAT3) were down-regulated under cold stress, while S-adenosylmethionine decarboxylase proenzyme 1 (AMD1) had an up-regulation tendency in response to cold stress in the two samples. Moreover, the delta-1-pyrroline-5-carboxylate synthase (P5CS), δ-ornithine aminotransferase (δ-OAT), alanine-glyoxylate transaminase (AGXT), branched-chain-amino-acid transaminase (ilvE), alpha-aminoadipic semialdehyde synthase (AASS), Tyrosine aminotransferase (TAT) and arginine decarboxylase related to amino acid metabolism were identified in two cultivars variously expressed under cold stress. The above DEGs related to amino acid metabolism were suspected to the reason for amino acids content change. The RNA-seq data were validated by real-time quantitative RT-PCR of 19 randomly selected genes. The findings of our study provide the gene expression profile between two varieties of winter turnip rape, which lay the foundation for a deeper understanding of the highly complex regulatory mechanisms in plants during cold treatment.

The effect of red and violet light emitting diode (LED) treatments on the postharvest quality and biodiversity of fresh-cut pakchoi (Brassica rapa L. Chinensis)

2021 ◽  
pp. 108201322110188
Author(s):  
Yuchen Zhang ◽  
Jing Xie
Keyword(s):  
Antioxidant Enzymes ◽  
Shelf Life ◽  
Brassica Rapa ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
Violet Light ◽  
Fresh Cut ◽  
Led Irradiation

The effects of red and violet Light Emitting Diode (LED) irradiation on the quality changes of fresh cut pakchoi ( Brassica rapa L.Chinensis), such as water migration, soluble solids, chlorophyll, ASA, shelf life, antioxidant enzyme activity and changes of biodiversity were evaluated in this study using physicochemical and high-throughput sequencing analys. The results showed that red and violet LED irradiation (15 μ mol/(m2 · s)) (1) are significantly inhibited the changes of sensory evaluation, (2) increased the content of chlorophyll, ASA and antioxidant enzymes, and (3) prolonged the shelf life of pakchoi at 4 °C. Furthermore, through the using of high-throughput sequencing, aerobic plate count and the count of Pseudomonas spp ., it was found that (4) red and violet LED changed the microbial community structure among samples, and inhibited the reproduction of specific spoilage organism (SSO) in fresh cut pakchoi. At the same time, compared with the traditional sterilization method, the results showed that visible light sterilization was also effective. In general, the results showed that LED treatment was an effective way to delay the senescence and maintain the quality of the pakchoi by enhancing the activity of antioxidant enzymes and regulating chlorophyll and ASA metabolism, inhibited the reproduction of SSO.

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